Prolixibacteraceae bacterium WC007

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Maribellus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6JYM3|A0A6I6JYM3_9BACT Xylose isomerase OS=Prolixibacteraceae bacterium WC007 OX=2681766 GN=xylA PE=3 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84VKK4 pKa = 10.18TEE6 pKa = 3.84TFKK9 pKa = 11.51NMKK12 pKa = 9.47KK13 pKa = 9.05WFFYY17 pKa = 9.27AWAVVTVLFASCEE30 pKa = 3.98GLTPGTGTEE39 pKa = 4.18EE40 pKa = 4.55EE41 pKa = 4.26EE42 pKa = 4.22QQDD45 pKa = 3.94VVNEE49 pKa = 4.07EE50 pKa = 4.35TIEE53 pKa = 3.98EE54 pKa = 4.28AVTNNAGDD62 pKa = 4.08HH63 pKa = 7.52DD64 pKa = 4.44DD65 pKa = 3.88TADD68 pKa = 3.69YY69 pKa = 9.22TWDD72 pKa = 3.89SNSVTQISLNGSSATEE88 pKa = 3.94SSDD91 pKa = 3.94DD92 pKa = 3.61VTIEE96 pKa = 3.89SGNIVIGAAGNYY108 pKa = 9.23SFSGTLSNGQIIVNTEE124 pKa = 3.53DD125 pKa = 3.46EE126 pKa = 4.72EE127 pKa = 4.42IVRR130 pKa = 11.84LILDD134 pKa = 4.2GVDD137 pKa = 3.8ISCSNSAPIYY147 pKa = 9.67IKK149 pKa = 10.59SAEE152 pKa = 4.17KK153 pKa = 10.83VLIALNEE160 pKa = 3.95NTTNKK165 pKa = 8.89LTDD168 pKa = 3.86GSSYY172 pKa = 11.36NYY174 pKa = 10.24DD175 pKa = 3.53DD176 pKa = 5.71EE177 pKa = 5.52EE178 pKa = 5.86DD179 pKa = 4.07EE180 pKa = 4.74EE181 pKa = 4.51PNAAIFSKK189 pKa = 10.95SDD191 pKa = 3.12LTIFGEE197 pKa = 4.54GTLVVDD203 pKa = 4.92ANFNDD208 pKa = 5.64GITSKK213 pKa = 10.89DD214 pKa = 3.32GLIIASGNISVTAVDD229 pKa = 3.99DD230 pKa = 4.91GIRR233 pKa = 11.84GKK235 pKa = 10.58DD236 pKa = 3.33YY237 pKa = 10.96IIIKK241 pKa = 10.05NGNFTVEE248 pKa = 3.92SDD250 pKa = 3.47GDD252 pKa = 4.07GLKK255 pKa = 10.39SDD257 pKa = 4.64NDD259 pKa = 3.79SDD261 pKa = 4.1DD262 pKa = 3.57SKK264 pKa = 11.75GYY266 pKa = 10.26IEE268 pKa = 5.5IYY270 pKa = 10.58DD271 pKa = 3.66GTFDD275 pKa = 4.48ISAKK279 pKa = 9.97GDD281 pKa = 3.51AVSAEE286 pKa = 4.0TDD288 pKa = 3.38LMIEE292 pKa = 4.03YY293 pKa = 10.86AEE295 pKa = 4.55IEE297 pKa = 4.37LTTSGSTSSYY307 pKa = 11.01YY308 pKa = 10.68EE309 pKa = 4.04STSSKK314 pKa = 10.46GLKK317 pKa = 10.12AGVNIIIDD325 pKa = 3.83DD326 pKa = 4.9GIFSLNCADD335 pKa = 5.66DD336 pKa = 5.73AIHH339 pKa = 6.78SNQTITINSGTYY351 pKa = 9.46EE352 pKa = 4.11ISSGDD357 pKa = 3.76DD358 pKa = 4.38GIHH361 pKa = 6.61SDD363 pKa = 3.94YY364 pKa = 11.66DD365 pKa = 3.68LVINDD370 pKa = 3.76GDD372 pKa = 3.89INITKK377 pKa = 9.62SYY379 pKa = 10.57EE380 pKa = 4.36GIEE383 pKa = 4.15SGQGDD388 pKa = 3.84MEE390 pKa = 4.76INKK393 pKa = 8.39GTIHH397 pKa = 6.69IKK399 pKa = 10.73SSDD402 pKa = 3.44DD403 pKa = 4.38GINLSAGGDD412 pKa = 3.47AMGGGGPWGGGSTSSGNYY430 pKa = 9.29YY431 pKa = 9.99IYY433 pKa = 10.63INGAYY438 pKa = 9.28IYY440 pKa = 10.13INASGDD446 pKa = 3.76GLDD449 pKa = 3.56SNGNIKK455 pKa = 8.02MTDD458 pKa = 3.02GTVLVDD464 pKa = 4.14GPTNSGNGALDD475 pKa = 3.81CNGSFTITGGLLIAAGSSGMAEE497 pKa = 4.31APASSSDD504 pKa = 3.57QYY506 pKa = 11.88SVLVKK511 pKa = 10.39FNSTKK516 pKa = 10.05SGGTIFHH523 pKa = 6.28VEE525 pKa = 4.03SEE527 pKa = 4.14KK528 pKa = 11.32GEE530 pKa = 4.31EE531 pKa = 3.75ILTYY535 pKa = 10.27KK536 pKa = 10.25PSKK539 pKa = 10.13NYY541 pKa = 10.22QSVAFSSPDD550 pKa = 3.25LTKK553 pKa = 10.93GEE555 pKa = 4.53SYY557 pKa = 10.77SIYY560 pKa = 10.21TGGSSTGDD568 pKa = 3.25EE569 pKa = 4.29TDD571 pKa = 3.47GLFSGGTYY579 pKa = 9.87TPGSVYY585 pKa = 10.64VSFDD589 pKa = 3.4ITSMTYY595 pKa = 9.91TIQQ598 pKa = 3.44

Molecular weight: 62.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6JWB1|A0A6I6JWB1_9BACT Mandelate racemase/muconate lactonizing enzyme family protein OS=Prolixibacteraceae bacterium WC007 OX=2681766 GN=GM418_18555 PE=4 SV=1
MM1 pKa = 7.93AKK3 pKa = 9.78RR4 pKa = 11.84ISIIIINLGIVVWGIIGFNHH24 pKa = 5.99LNYY27 pKa = 9.36WEE29 pKa = 4.17RR30 pKa = 11.84SARR33 pKa = 11.84IFQVNSEE40 pKa = 4.19QNFRR44 pKa = 11.84RR45 pKa = 11.84GFDD48 pKa = 2.86RR49 pKa = 11.84GRR51 pKa = 11.84PGFEE55 pKa = 5.24DD56 pKa = 3.66RR57 pKa = 11.84SSWDD61 pKa = 2.95RR62 pKa = 11.84STLTEE67 pKa = 5.24RR68 pKa = 11.84PDD70 pKa = 3.76LLNLSDD76 pKa = 6.04SIAQQSADD84 pKa = 3.11VVQRR88 pKa = 11.84NRR90 pKa = 11.84EE91 pKa = 4.24SIRR94 pKa = 11.84EE95 pKa = 4.02RR96 pKa = 11.84NSEE99 pKa = 3.7DD100 pKa = 2.85RR101 pKa = 11.84SYY103 pKa = 11.55GRR105 pKa = 11.84RR106 pKa = 11.84GFHH109 pKa = 6.4SRR111 pKa = 11.84QKK113 pKa = 8.86IQLRR117 pKa = 11.84NVVWFLAVFCLFTVGTIYY135 pKa = 10.44TDD137 pKa = 3.02KK138 pKa = 10.52TIRR141 pKa = 11.84AFRR144 pKa = 11.84KK145 pKa = 9.53KK146 pKa = 10.16EE147 pKa = 3.76KK148 pKa = 10.67

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6116

0

6116

2299017

32

11651

375.9

42.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.113 ± 0.046

0.855 ± 0.028

5.495 ± 0.052

7.0 ± 0.039

5.344 ± 0.028

6.726 ± 0.033

1.702 ± 0.015

7.582 ± 0.03

7.387 ± 0.054

8.949 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.018

5.985 ± 0.028

3.742 ± 0.02

3.35 ± 0.017

3.772 ± 0.028

6.488 ± 0.025

5.472 ± 0.073

6.28 ± 0.022

1.384 ± 0.014

4.144 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski