Amino acid dipepetide frequency for Prolixibacteraceae bacterium WC007

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.548AlaAla: 4.548 ± 0.162	0.657AlaCys: 0.657 ± 0.047	3.492AlaAsp: 3.492 ± 0.084	4.401AlaGlu: 4.401 ± 0.059	3.183AlaPhe: 3.183 ± 0.048	4.926AlaGly: 4.926 ± 0.058	1.007AlaHis: 1.007 ± 0.023	4.461AlaIle: 4.461 ± 0.053	3.923AlaLys: 3.923 ± 0.055	5.485AlaLeu: 5.485 ± 0.064	1.357AlaMet: 1.357 ± 0.027	3.02AlaAsn: 3.02 ± 0.068	1.949AlaPro: 1.949 ± 0.039	2.284AlaGln: 2.284 ± 0.073	2.245AlaArg: 2.245 ± 0.036	3.757AlaSer: 3.757 ± 0.052	3.237AlaThr: 3.237 ± 0.054	4.137AlaVal: 4.137 ± 0.052	0.853AlaTrp: 0.853 ± 0.04	2.205AlaTyr: 2.205 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.529CysAla: 0.529 ± 0.043	0.108CysCys: 0.108 ± 0.007	0.616CysAsp: 0.616 ± 0.086	0.5CysGlu: 0.5 ± 0.021	0.465CysPhe: 0.465 ± 0.014	0.7CysGly: 0.7 ± 0.042	0.203CysHis: 0.203 ± 0.012	0.592CysIle: 0.592 ± 0.017	0.502CysLys: 0.502 ± 0.014	0.654CysLeu: 0.654 ± 0.018	0.167CysMet: 0.167 ± 0.009	0.486CysAsn: 0.486 ± 0.02	0.408CysPro: 0.408 ± 0.022	0.248CysGln: 0.248 ± 0.011	0.33CysArg: 0.33 ± 0.012	0.661CysSer: 0.661 ± 0.05	0.475CysThr: 0.475 ± 0.023	0.468CysVal: 0.468 ± 0.017	0.103CysTrp: 0.103 ± 0.007	0.339CysTyr: 0.339 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.451AspAla: 3.451 ± 0.084	0.477AspCys: 0.477 ± 0.028	2.965AspAsp: 2.965 ± 0.06	4.103AspGlu: 4.103 ± 0.047	3.764AspPhe: 3.764 ± 0.097	3.945AspGly: 3.945 ± 0.064	0.769AspHis: 0.769 ± 0.019	4.27AspIle: 4.27 ± 0.058	3.906AspLys: 3.906 ± 0.049	4.871AspLeu: 4.871 ± 0.045	1.152AspMet: 1.152 ± 0.02	3.157AspAsn: 3.157 ± 0.099	1.93AspPro: 1.93 ± 0.033	1.404AspGln: 1.404 ± 0.032	1.858AspArg: 1.858 ± 0.034	3.227AspSer: 3.227 ± 0.047	2.537AspThr: 2.537 ± 0.119	3.446AspVal: 3.446 ± 0.045	1.002AspTrp: 1.002 ± 0.04	2.719AspTyr: 2.719 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	3.986GluAla: 3.986 ± 0.052	0.428GluCys: 0.428 ± 0.025	3.334GluAsp: 3.334 ± 0.043	5.23GluGlu: 5.23 ± 0.071	3.152GluPhe: 3.152 ± 0.039	4.027GluGly: 4.027 ± 0.043	1.093GluHis: 1.093 ± 0.028	6.196GluIle: 6.196 ± 0.06	6.748GluLys: 6.748 ± 0.082	6.317GluLeu: 6.317 ± 0.066	1.94GluMet: 1.94 ± 0.035	5.331GluAsn: 5.331 ± 0.055	1.98GluPro: 1.98 ± 0.041	2.372GluGln: 2.372 ± 0.04	2.646GluArg: 2.646 ± 0.048	3.69GluSer: 3.69 ± 0.047	3.836GluThr: 3.836 ± 0.045	4.097GluVal: 4.097 ± 0.052	0.893GluTrp: 0.893 ± 0.024	2.7GluTyr: 2.7 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.087PheAla: 3.087 ± 0.047	0.586PheCys: 0.586 ± 0.04	3.39PheAsp: 3.39 ± 0.072	3.515PheGlu: 3.515 ± 0.049	2.976PhePhe: 2.976 ± 0.051	3.457PheGly: 3.457 ± 0.043	0.93PheHis: 0.93 ± 0.023	3.972PheIle: 3.972 ± 0.053	3.152PheLys: 3.152 ± 0.038	4.876PheLeu: 4.876 ± 0.07	1.16PheMet: 1.16 ± 0.026	3.056PheAsn: 3.056 ± 0.04	1.942PhePro: 1.942 ± 0.031	1.61PheGln: 1.61 ± 0.029	2.067PheArg: 2.067 ± 0.036	4.334PheSer: 4.334 ± 0.053	2.922PheThr: 2.922 ± 0.037	3.324PheVal: 3.324 ± 0.046	0.725PheTrp: 0.725 ± 0.021	2.356PheTyr: 2.356 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	3.963GlyAla: 3.963 ± 0.051	0.751GlyCys: 0.751 ± 0.045	3.475GlyAsp: 3.475 ± 0.05	4.537GlyGlu: 4.537 ± 0.051	3.73GlyPhe: 3.73 ± 0.051	4.896GlyGly: 4.896 ± 0.121	1.145GlyHis: 1.145 ± 0.023	5.684GlyIle: 5.684 ± 0.063	5.429GlyLys: 5.429 ± 0.057	5.689GlyLeu: 5.689 ± 0.071	1.746GlyMet: 1.746 ± 0.036	3.925GlyAsn: 3.925 ± 0.057	1.523GlyPro: 1.523 ± 0.029	1.93GlyGln: 1.93 ± 0.034	2.474GlyArg: 2.474 ± 0.036	4.02GlySer: 4.02 ± 0.052	3.953GlyThr: 3.953 ± 0.056	4.255GlyVal: 4.255 ± 0.064	1.061GlyTrp: 1.061 ± 0.02	3.069GlyTyr: 3.069 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.914HisAla: 0.914 ± 0.022	0.189HisCys: 0.189 ± 0.009	0.795HisAsp: 0.795 ± 0.019	0.95HisGlu: 0.95 ± 0.025	1.142HisPhe: 1.142 ± 0.027	1.077HisGly: 1.077 ± 0.025	0.445HisHis: 0.445 ± 0.016	1.251HisIle: 1.251 ± 0.025	1.07HisLys: 1.07 ± 0.02	1.694HisLeu: 1.694 ± 0.03	0.346HisMet: 0.346 ± 0.012	0.91HisAsn: 0.91 ± 0.021	0.99HisPro: 0.99 ± 0.025	0.62HisGln: 0.62 ± 0.016	0.689HisArg: 0.689 ± 0.019	1.138HisSer: 1.138 ± 0.024	0.813HisThr: 0.813 ± 0.018	0.908HisVal: 0.908 ± 0.019	0.301HisTrp: 0.301 ± 0.012	0.782HisTyr: 0.782 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	4.721IleAla: 4.721 ± 0.061	0.716IleCys: 0.716 ± 0.05	4.517IleAsp: 4.517 ± 0.056	5.194IleGlu: 5.194 ± 0.058	3.899IlePhe: 3.899 ± 0.051	4.785IleGly: 4.785 ± 0.06	1.399IleHis: 1.399 ± 0.025	5.473IleIle: 5.473 ± 0.068	5.187IleLys: 5.187 ± 0.063	6.933IleLeu: 6.933 ± 0.088	1.281IleMet: 1.281 ± 0.025	4.263IleAsn: 4.263 ± 0.054	3.377IlePro: 3.377 ± 0.04	2.618IleGln: 2.618 ± 0.035	3.062IleArg: 3.062 ± 0.041	5.774IleSer: 5.774 ± 0.062	4.344IleThr: 4.344 ± 0.113	4.528IleVal: 4.528 ± 0.057	0.875IleTrp: 0.875 ± 0.021	2.879IleTyr: 2.879 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.148LysAla: 4.148 ± 0.057	0.407LysCys: 0.407 ± 0.015	3.742LysAsp: 3.742 ± 0.044	5.835LysGlu: 5.835 ± 0.066	2.783LysPhe: 2.783 ± 0.041	4.203LysGly: 4.203 ± 0.05	1.177LysHis: 1.177 ± 0.023	6.226LysIle: 6.226 ± 0.074	6.313LysLys: 6.313 ± 0.081	6.168LysLeu: 6.168 ± 0.071	2.044LysMet: 2.044 ± 0.033	5.251LysAsn: 5.251 ± 0.059	2.468LysPro: 2.468 ± 0.039	2.613LysGln: 2.613 ± 0.042	2.953LysArg: 2.953 ± 0.046	4.359LysSer: 4.359 ± 0.052	4.556LysThr: 4.556 ± 0.052	4.388LysVal: 4.388 ± 0.048	0.978LysTrp: 0.978 ± 0.021	3.255LysTyr: 3.255 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	5.71LeuAla: 5.71 ± 0.06	0.649LeuCys: 0.649 ± 0.02	4.56LeuAsp: 4.56 ± 0.043	5.797LeuGlu: 5.797 ± 0.06	5.112LeuPhe: 5.112 ± 0.062	5.471LeuGly: 5.471 ± 0.062	1.471LeuHis: 1.471 ± 0.029	6.461LeuIle: 6.461 ± 0.068	7.3LeuLys: 7.3 ± 0.081	8.404LeuLeu: 8.404 ± 0.091	1.965LeuMet: 1.965 ± 0.034	5.552LeuAsn: 5.552 ± 0.055	3.713LeuPro: 3.713 ± 0.049	3.021LeuGln: 3.021 ± 0.04	3.198LeuArg: 3.198 ± 0.047	6.458LeuSer: 6.458 ± 0.06	5.029LeuThr: 5.029 ± 0.11	5.397LeuVal: 5.397 ± 0.059	1.0LeuTrp: 1.0 ± 0.023	3.225LeuTyr: 3.225 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	1.608MetAla: 1.608 ± 0.032	0.141MetCys: 0.141 ± 0.008	1.203MetAsp: 1.203 ± 0.026	1.557MetGlu: 1.557 ± 0.03	0.909MetPhe: 0.909 ± 0.025	1.553MetGly: 1.553 ± 0.028	0.362MetHis: 0.362 ± 0.013	1.485MetIle: 1.485 ± 0.033	2.325MetLys: 2.325 ± 0.034	1.916MetLeu: 1.916 ± 0.033	0.563MetMet: 0.563 ± 0.017	1.391MetAsn: 1.391 ± 0.029	0.937MetPro: 0.937 ± 0.022	0.786MetGln: 0.786 ± 0.023	0.9MetArg: 0.9 ± 0.022	1.199MetSer: 1.199 ± 0.024	1.048MetThr: 1.048 ± 0.02	1.467MetVal: 1.467 ± 0.029	0.225MetTrp: 0.225 ± 0.01	0.736MetTyr: 0.736 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.431AsnAla: 3.431 ± 0.071	0.554AsnCys: 0.554 ± 0.034	3.109AsnAsp: 3.109 ± 0.058	3.869AsnGlu: 3.869 ± 0.052	3.009AsnPhe: 3.009 ± 0.037	4.112AsnGly: 4.112 ± 0.053	1.056AsnHis: 1.056 ± 0.026	4.77AsnIle: 4.77 ± 0.045	4.056AsnLys: 4.056 ± 0.054	5.343AsnLeu: 5.343 ± 0.065	1.297AsnMet: 1.297 ± 0.026	3.519AsnAsn: 3.519 ± 0.057	2.956AsnPro: 2.956 ± 0.047	2.204AsnGln: 2.204 ± 0.034	2.48AsnArg: 2.48 ± 0.041	3.966AsnSer: 3.966 ± 0.057	3.194AsnThr: 3.194 ± 0.045	3.248AsnVal: 3.248 ± 0.044	0.919AsnTrp: 0.919 ± 0.023	2.762AsnTyr: 2.762 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.413ProAla: 2.413 ± 0.057	0.192ProCys: 0.192 ± 0.01	2.677ProAsp: 2.677 ± 0.037	3.796ProGlu: 3.796 ± 0.049	2.073ProPhe: 2.073 ± 0.036	2.928ProGly: 2.928 ± 0.04	0.688ProHis: 0.688 ± 0.018	2.025ProIle: 2.025 ± 0.035	2.061ProLys: 2.061 ± 0.03	3.125ProLeu: 3.125 ± 0.047	0.689ProMet: 0.689 ± 0.02	1.914ProAsn: 1.914 ± 0.027	1.153ProPro: 1.153 ± 0.065	1.226ProGln: 1.226 ± 0.022	1.128ProArg: 1.128 ± 0.025	2.12ProSer: 2.12 ± 0.03	1.836ProThr: 1.836 ± 0.051	3.435ProVal: 3.435 ± 0.065	0.478ProTrp: 0.478 ± 0.015	1.463ProTyr: 1.463 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	1.916GlnAla: 1.916 ± 0.043	0.184GlnCys: 0.184 ± 0.009	1.403GlnAsp: 1.403 ± 0.027	2.156GlnGlu: 2.156 ± 0.035	1.606GlnPhe: 1.606 ± 0.027	1.791GlnGly: 1.791 ± 0.035	0.546GlnHis: 0.546 ± 0.016	2.707GlnIle: 2.707 ± 0.036	3.184GlnLys: 3.184 ± 0.05	3.246GlnLeu: 3.246 ± 0.047	0.866GlnMet: 0.866 ± 0.021	2.428GlnAsn: 2.428 ± 0.044	1.324GlnPro: 1.324 ± 0.041	1.397GlnGln: 1.397 ± 0.031	1.301GlnArg: 1.301 ± 0.024	1.945GlnSer: 1.945 ± 0.03	1.967GlnThr: 1.967 ± 0.051	1.917GlnVal: 1.917 ± 0.029	0.421GlnTrp: 0.421 ± 0.016	1.195GlnTyr: 1.195 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	2.07ArgAla: 2.07 ± 0.037	0.271ArgCys: 0.271 ± 0.012	1.879ArgAsp: 1.879 ± 0.032	2.763ArgGlu: 2.763 ± 0.047	2.187ArgPhe: 2.187 ± 0.033	2.194ArgGly: 2.194 ± 0.038	0.615ArgHis: 0.615 ± 0.018	3.214ArgIle: 3.214 ± 0.043	3.152ArgLys: 3.152 ± 0.044	3.403ArgLeu: 3.403 ± 0.047	0.999ArgMet: 0.999 ± 0.024	2.421ArgAsn: 2.421 ± 0.036	1.24ArgPro: 1.24 ± 0.025	1.281ArgGln: 1.281 ± 0.031	1.525ArgArg: 1.525 ± 0.034	2.059ArgSer: 2.059 ± 0.037	1.925ArgThr: 1.925 ± 0.031	2.159ArgVal: 2.159 ± 0.032	0.582ArgTrp: 0.582 ± 0.018	1.781ArgTyr: 1.781 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	3.922SerAla: 3.922 ± 0.047	0.717SerCys: 0.717 ± 0.025	3.726SerAsp: 3.726 ± 0.073	4.583SerGlu: 4.583 ± 0.053	3.789SerPhe: 3.789 ± 0.042	5.254SerGly: 5.254 ± 0.064	1.08SerHis: 1.08 ± 0.021	4.545SerIle: 4.545 ± 0.055	4.104SerLys: 4.104 ± 0.05	5.732SerLeu: 5.732 ± 0.059	1.338SerMet: 1.338 ± 0.025	3.395SerAsn: 3.395 ± 0.053	2.477SerPro: 2.477 ± 0.044	2.033SerGln: 2.033 ± 0.028	2.358SerArg: 2.358 ± 0.039	4.231SerSer: 4.231 ± 0.06	3.19SerThr: 3.19 ± 0.043	4.56SerVal: 4.56 ± 0.05	1.005SerTrp: 1.005 ± 0.029	2.838SerTyr: 2.838 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.521ThrAla: 3.521 ± 0.109	0.41ThrCys: 0.41 ± 0.02	3.333ThrAsp: 3.333 ± 0.081	3.573ThrGlu: 3.573 ± 0.048	2.757ThrPhe: 2.757 ± 0.038	4.548ThrGly: 4.548 ± 0.053	0.911ThrHis: 0.911 ± 0.021	4.314ThrIle: 4.314 ± 0.082	3.205ThrLys: 3.205 ± 0.045	4.762ThrLeu: 4.762 ± 0.086	0.993ThrMet: 0.993 ± 0.021	2.833ThrAsn: 2.833 ± 0.045	2.341ThrPro: 2.341 ± 0.032	1.88ThrGln: 1.88 ± 0.085	1.886ThrArg: 1.886 ± 0.026	3.434ThrSer: 3.434 ± 0.052	3.239ThrThr: 3.239 ± 0.101	3.905ThrVal: 3.905 ± 0.163	0.781ThrTrp: 0.781 ± 0.052	2.095ThrTyr: 2.095 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.099ValAla: 4.099 ± 0.055	0.604ValCys: 0.604 ± 0.018	3.562ValAsp: 3.562 ± 0.052	4.206ValGlu: 4.206 ± 0.047	3.686ValPhe: 3.686 ± 0.048	3.676ValGly: 3.676 ± 0.051	0.98ValHis: 0.98 ± 0.022	4.533ValIle: 4.533 ± 0.059	4.412ValLys: 4.412 ± 0.049	5.832ValLeu: 5.832 ± 0.058	1.233ValMet: 1.233 ± 0.026	3.546ValAsn: 3.546 ± 0.052	2.505ValPro: 2.505 ± 0.058	1.881ValGln: 1.881 ± 0.028	2.236ValArg: 2.236 ± 0.035	4.588ValSer: 4.588 ± 0.056	3.604ValThr: 3.604 ± 0.096	4.348ValVal: 4.348 ± 0.055	0.779ValTrp: 0.779 ± 0.021	2.492ValTyr: 2.492 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.776TrpAla: 0.776 ± 0.02	0.137TrpCys: 0.137 ± 0.008	0.888TrpAsp: 0.888 ± 0.037	1.002TrpGlu: 1.002 ± 0.026	0.685TrpPhe: 0.685 ± 0.019	0.987TrpGly: 0.987 ± 0.026	0.322TrpHis: 0.322 ± 0.014	0.94TrpIle: 0.94 ± 0.02	1.015TrpLys: 1.015 ± 0.025	1.236TrpLeu: 1.236 ± 0.039	0.375TrpMet: 0.375 ± 0.016	0.85TrpAsn: 0.85 ± 0.023	0.418TrpPro: 0.418 ± 0.015	0.588TrpGln: 0.588 ± 0.018	0.563TrpArg: 0.563 ± 0.016	0.763TrpSer: 0.763 ± 0.02	0.764TrpThr: 0.764 ± 0.049	0.755TrpVal: 0.755 ± 0.016	0.24TrpTrp: 0.24 ± 0.011	0.533TrpTyr: 0.533 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.314TyrAla: 2.314 ± 0.036	0.374TyrCys: 0.374 ± 0.014	2.284TyrAsp: 2.284 ± 0.036	2.428TyrGlu: 2.428 ± 0.035	2.531TyrPhe: 2.531 ± 0.042	2.726TyrGly: 2.726 ± 0.046	0.826TyrHis: 0.826 ± 0.015	2.707TyrIle: 2.707 ± 0.047	2.821TyrLys: 2.821 ± 0.039	3.808TyrLeu: 3.808 ± 0.052	0.801TyrMet: 0.801 ± 0.019	2.605TyrAsn: 2.605 ± 0.037	1.786TyrPro: 1.786 ± 0.029	1.506TyrGln: 1.506 ± 0.029	1.82TyrArg: 1.82 ± 0.03	3.157TyrSer: 3.157 ± 0.048	2.254TyrThr: 2.254 ± 0.055	2.059TyrVal: 2.059 ± 0.036	0.618TyrTrp: 0.618 ± 0.018	2.011TyrTyr: 2.011 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6116 proteins (2299017 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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