Sulfurimicrobium lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sulfuricellaceae; Sulfurimicrobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F8VC16|A0A6F8VC16_9PROT Cytochrome d ubiquinol oxidase subunit I OS=Sulfurimicrobium lacus OX=2715678 GN=SKTS_17560 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.21KK3 pKa = 9.53YY4 pKa = 10.5QCVVCGWIYY13 pKa = 11.21DD14 pKa = 4.14EE15 pKa = 4.65VAGAPDD21 pKa = 3.59EE22 pKa = 5.32GIAPGTRR29 pKa = 11.84WDD31 pKa = 4.46DD32 pKa = 4.04VPDD35 pKa = 3.21SWACPDD41 pKa = 4.01CGVSKK46 pKa = 11.14SDD48 pKa = 3.74FEE50 pKa = 4.39MVAII54 pKa = 4.96

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F8VEG1|A0A6F8VEG1_9PROT 6-phosphogluconolactonase OS=Sulfurimicrobium lacus OX=2715678 GN=nagB PE=3 SV=1
MM1 pKa = 8.5DD2 pKa = 5.82DD3 pKa = 3.64RR4 pKa = 11.84QAQNFWPPLAAGIGLGLALMAMFLFTGHH32 pKa = 6.91GLGANGFFTRR42 pKa = 11.84TVVWLSDD49 pKa = 2.91IANPVWAEE57 pKa = 3.56VNAYY61 pKa = 7.7FKK63 pKa = 10.6PYY65 pKa = 9.69LGKK68 pKa = 10.56GHH70 pKa = 7.49PLNEE74 pKa = 4.27WISWEE79 pKa = 4.1IIGVALGALVGSLTARR95 pKa = 11.84RR96 pKa = 11.84FQWKK100 pKa = 9.49IEE102 pKa = 3.97RR103 pKa = 11.84GPRR106 pKa = 11.84TGVPARR112 pKa = 11.84LALALGGGVLTGFGAPLARR131 pKa = 11.84GCTSGLGLSGGATLAVAAFVFLIAFFSAGLFLSALTRR168 pKa = 11.84RR169 pKa = 11.84IWQQ172 pKa = 3.37

Molecular weight: 18.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3673

0

3673

1167837

39

2870

318.0

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.104 ± 0.044

0.979 ± 0.018

5.327 ± 0.028

6.087 ± 0.044

3.746 ± 0.024

7.817 ± 0.037

2.389 ± 0.021

5.131 ± 0.028

4.211 ± 0.036

10.831 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.02

3.146 ± 0.028

4.73 ± 0.03

4.024 ± 0.023

6.261 ± 0.041

5.563 ± 0.029

4.942 ± 0.038

7.16 ± 0.033

1.322 ± 0.016

2.59 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski