Tomato leaf curl Liwa virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0UQL1|W0UQL1_9GEMI C4 protein OS=Tomato leaf curl Liwa virus OX=1303385 GN=C4 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.55FPEE11 pKa = 4.6SVHH14 pKa = 6.55GFRR17 pKa = 11.84CMLAVKK23 pKa = 9.91YY24 pKa = 10.12LQLVEE29 pKa = 4.7NSYY32 pKa = 11.65SPDD35 pKa = 3.11TLGYY39 pKa = 10.84DD40 pKa = 4.83LIRR43 pKa = 11.84DD44 pKa = 4.71LISVIRR50 pKa = 11.84ARR52 pKa = 11.84NYY54 pKa = 9.49VEE56 pKa = 3.52ATSRR60 pKa = 11.84YY61 pKa = 6.98NHH63 pKa = 5.62FHH65 pKa = 6.55ARR67 pKa = 11.84LEE69 pKa = 4.32GTPSSQLRR77 pKa = 11.84QPICQPCCCPHH88 pKa = 6.64CPRR91 pKa = 11.84HH92 pKa = 5.95KK93 pKa = 10.43GKK95 pKa = 10.89GMGQQAHH102 pKa = 6.01EE103 pKa = 4.79SEE105 pKa = 4.48AQDD108 pKa = 3.34VQNVQKK114 pKa = 10.64PP115 pKa = 3.36

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0USM5|W0USM5_9GEMI Transcriptional activator protein OS=Tomato leaf curl Liwa virus OX=1303385 GN=C2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 2.99SPYY27 pKa = 10.47ASRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.71AKK42 pKa = 10.29AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 9.32VQSYY77 pKa = 7.04EE78 pKa = 3.67QRR80 pKa = 11.84DD81 pKa = 4.2DD82 pKa = 3.51IKK84 pKa = 9.71HH85 pKa = 5.35TGIVRR90 pKa = 11.84CVSDD94 pKa = 3.31VTRR97 pKa = 11.84GSGITHH103 pKa = 6.35RR104 pKa = 11.84VGKK107 pKa = 9.5RR108 pKa = 11.84FCVKK112 pKa = 10.37SIYY115 pKa = 10.32FLGKK119 pKa = 9.15VWMDD123 pKa = 3.41EE124 pKa = 4.15NIKK127 pKa = 10.04KK128 pKa = 9.97QNHH131 pKa = 4.95TNQVMFFLVRR141 pKa = 11.84DD142 pKa = 3.7RR143 pKa = 11.84RR144 pKa = 11.84PYY146 pKa = 10.66GSSPMDD152 pKa = 3.83FGQVFNMFDD161 pKa = 3.99NEE163 pKa = 4.11PSTATVKK170 pKa = 10.85NDD172 pKa = 2.82LRR174 pKa = 11.84DD175 pKa = 3.36RR176 pKa = 11.84FQVMRR181 pKa = 11.84KK182 pKa = 7.65FHH184 pKa = 6.03ATVIGGPSGMKK195 pKa = 9.5EE196 pKa = 3.54QALVKK201 pKa = 10.31RR202 pKa = 11.84FFRR205 pKa = 11.84INSHH209 pKa = 3.89VTYY212 pKa = 10.54NHH214 pKa = 5.79QEE216 pKa = 3.49AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.67FYY252 pKa = 11.26DD253 pKa = 3.51SVSNN257 pKa = 3.9

Molecular weight: 29.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1089

85

362

181.5

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.152 ± 0.707

2.112 ± 0.438

4.867 ± 0.468

4.224 ± 0.545

4.5 ± 0.57

5.051 ± 0.575

4.04 ± 0.557

5.234 ± 0.55

5.693 ± 0.757

6.52 ± 0.857

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.7

5.877 ± 0.813

6.244 ± 0.713

5.142 ± 0.671

6.703 ± 1.306

8.815 ± 1.466

4.867 ± 0.963

6.336 ± 0.824

1.194 ± 0.215

3.673 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski