Bacillus phage SP-15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Thornevirus; Bacillus virus SP15

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 317 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127AWH1|A0A127AWH1_9CAUD Uncharacterized protein OS=Bacillus phage SP-15 OX=1792032 GN=SP15_134 PE=4 SV=1
MM1 pKa = 8.04PEE3 pKa = 3.7LQIAEE8 pKa = 4.3SHH10 pKa = 6.1GLQDD14 pKa = 3.25VLVFQLCDD22 pKa = 3.77CDD24 pKa = 4.84SVAAYY29 pKa = 8.93TLDD32 pKa = 4.84EE33 pKa = 5.35AIDD36 pKa = 3.59WYY38 pKa = 11.4KK39 pKa = 11.29NLTGLTDD46 pKa = 4.04DD47 pKa = 4.12EE48 pKa = 5.33LYY50 pKa = 10.27PYY52 pKa = 10.79DD53 pKa = 4.69EE54 pKa = 5.26IEE56 pKa = 4.46TVPFDD61 pKa = 3.6YY62 pKa = 10.3QVRR65 pKa = 11.84ISEE68 pKa = 4.28SDD70 pKa = 3.35STLTTVRR77 pKa = 11.84QIVDD81 pKa = 3.69QLWDD85 pKa = 3.56GTPFIVTSKK94 pKa = 10.98GVV96 pKa = 2.99

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127AWQ3|A0A127AWQ3_9CAUD Uncharacterized protein OS=Bacillus phage SP-15 OX=1792032 GN=SP15_217 PE=4 SV=1
MM1 pKa = 7.03LQARR5 pKa = 11.84STEE8 pKa = 4.03KK9 pKa = 9.93LHH11 pKa = 6.96SYY13 pKa = 7.92KK14 pKa = 10.54TSTRR18 pKa = 11.84KK19 pKa = 8.43GTHH22 pKa = 5.6LRR24 pKa = 11.84LVQSQTNTTPVRR36 pKa = 11.84QRR38 pKa = 11.84KK39 pKa = 8.95SIKK42 pKa = 9.64SRR44 pKa = 11.84IMNRR48 pKa = 11.84LDD50 pKa = 2.85TWADD54 pKa = 3.59SISQKK59 pKa = 8.74TFNRR63 pKa = 11.84IMISIFGIVIVSLILLASHH82 pKa = 7.28LDD84 pKa = 3.44YY85 pKa = 10.74VTYY88 pKa = 11.19LEE90 pKa = 4.72INGKK94 pKa = 9.24

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

317

0

317

67461

27

4401

212.8

24.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.415 ± 0.203

0.882 ± 0.073

6.813 ± 0.249

7.653 ± 0.157

3.691 ± 0.109

6.189 ± 0.147

1.958 ± 0.072

6.862 ± 0.188

7.255 ± 0.284

8.552 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.132

5.087 ± 0.104

3.701 ± 0.126

3.206 ± 0.134

4.822 ± 0.117

7.384 ± 0.259

5.547 ± 0.084

7.103 ± 0.165

1.047 ± 0.051

4.251 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski