Amino acid dipepetide frequency for Bacillus phage SP-15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.416AlaAla: 2.416 ± 0.259
0.356AlaCys: 0.356 ± 0.068
3.232AlaAsp: 3.232 ± 0.218
3.78AlaGlu: 3.78 ± 0.3
1.616AlaPhe: 1.616 ± 0.168
3.78AlaGly: 3.78 ± 0.343
0.8AlaHis: 0.8 ± 0.121
4.373AlaIle: 4.373 ± 0.244
4.773AlaLys: 4.773 ± 0.313
4.18AlaLeu: 4.18 ± 0.298
1.571AlaMet: 1.571 ± 0.206
2.772AlaAsn: 2.772 ± 0.25
1.868AlaPro: 1.868 ± 0.202
2.06AlaGln: 2.06 ± 0.202
2.683AlaArg: 2.683 ± 0.251
3.647AlaSer: 3.647 ± 0.308
3.602AlaThr: 3.602 ± 0.303
3.958AlaVal: 3.958 ± 0.282
0.4AlaTrp: 0.4 ± 0.087
2.283AlaTyr: 2.283 ± 0.171
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.054
0.074CysCys: 0.074 ± 0.054
0.934CysAsp: 0.934 ± 0.339
0.489CysGlu: 0.489 ± 0.115
0.267CysPhe: 0.267 ± 0.062
0.86CysGly: 0.86 ± 0.13
0.163CysHis: 0.163 ± 0.057
0.489CysIle: 0.489 ± 0.071
0.697CysLys: 0.697 ± 0.1
0.578CysLeu: 0.578 ± 0.1
0.296CysMet: 0.296 ± 0.067
0.445CysAsn: 0.445 ± 0.079
0.548CysPro: 0.548 ± 0.089
0.237CysGln: 0.237 ± 0.053
0.43CysArg: 0.43 ± 0.078
0.756CysSer: 0.756 ± 0.12
0.534CysThr: 0.534 ± 0.089
0.415CysVal: 0.415 ± 0.074
0.074CysTrp: 0.074 ± 0.028
0.296CysTyr: 0.296 ± 0.071
0.0CysXaa: 0.0 ± 0.0
Asp
3.098AspAla: 3.098 ± 0.196
0.786AspCys: 0.786 ± 0.153
3.81AspAsp: 3.81 ± 0.257
5.292AspGlu: 5.292 ± 0.359
2.713AspPhe: 2.713 ± 0.2
4.269AspGly: 4.269 ± 0.308
1.349AspHis: 1.349 ± 0.137
5.322AspIle: 5.322 ± 0.492
5.648AspLys: 5.648 ± 0.348
6.004AspLeu: 6.004 ± 0.32
1.927AspMet: 1.927 ± 0.183
3.306AspAsn: 3.306 ± 0.22
2.905AspPro: 2.905 ± 0.275
2.283AspGln: 2.283 ± 0.183
2.728AspArg: 2.728 ± 0.195
4.936AspSer: 4.936 ± 0.512
3.884AspThr: 3.884 ± 0.249
3.899AspVal: 3.899 ± 0.286
0.815AspTrp: 0.815 ± 0.147
3.143AspTyr: 3.143 ± 0.196
0.0AspXaa: 0.0 ± 0.0
Glu
4.803GluAla: 4.803 ± 0.309
0.623GluCys: 0.623 ± 0.103
5.159GluAsp: 5.159 ± 0.295
6.137GluGlu: 6.137 ± 0.377
3.854GluPhe: 3.854 ± 0.229
4.403GluGly: 4.403 ± 0.277
1.23GluHis: 1.23 ± 0.148
5.233GluIle: 5.233 ± 0.292
4.343GluLys: 4.343 ± 0.296
8.716GluLeu: 8.716 ± 0.379
2.046GluMet: 2.046 ± 0.202
3.261GluAsn: 3.261 ± 0.216
1.912GluPro: 1.912 ± 0.177
2.268GluGln: 2.268 ± 0.191
2.876GluArg: 2.876 ± 0.24
5.989GluSer: 5.989 ± 0.391
3.024GluThr: 3.024 ± 0.209
6.315GluVal: 6.315 ± 0.296
0.845GluTrp: 0.845 ± 0.104
3.498GluTyr: 3.498 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
1.527PheAla: 1.527 ± 0.18
0.311PheCys: 0.311 ± 0.069
2.905PheAsp: 2.905 ± 0.255
2.905PheGlu: 2.905 ± 0.262
1.008PhePhe: 1.008 ± 0.13
2.238PheGly: 2.238 ± 0.167
0.8PheHis: 0.8 ± 0.13
2.461PheIle: 2.461 ± 0.211
3.068PheLys: 3.068 ± 0.244
2.95PheLeu: 2.95 ± 0.225
1.008PheMet: 1.008 ± 0.125
2.253PheAsn: 2.253 ± 0.214
1.082PhePro: 1.082 ± 0.122
1.008PheGln: 1.008 ± 0.141
2.046PheArg: 2.046 ± 0.196
2.742PheSer: 2.742 ± 0.216
2.298PheThr: 2.298 ± 0.2
2.357PheVal: 2.357 ± 0.204
0.4PheTrp: 0.4 ± 0.084
1.542PheTyr: 1.542 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
2.698GlyAla: 2.698 ± 0.309
0.534GlyCys: 0.534 ± 0.088
4.076GlyAsp: 4.076 ± 0.304
4.121GlyGlu: 4.121 ± 0.273
2.55GlyPhe: 2.55 ± 0.181
3.854GlyGly: 3.854 ± 0.386
1.304GlyHis: 1.304 ± 0.149
4.566GlyIle: 4.566 ± 0.286
4.566GlyLys: 4.566 ± 0.287
4.744GlyLeu: 4.744 ± 0.33
1.616GlyMet: 1.616 ± 0.183
3.217GlyAsn: 3.217 ± 0.254
1.008GlyPro: 1.008 ± 0.139
2.401GlyGln: 2.401 ± 0.247
3.587GlyArg: 3.587 ± 0.226
4.684GlySer: 4.684 ± 0.412
4.343GlyThr: 4.343 ± 0.347
4.536GlyVal: 4.536 ± 0.26
0.756GlyTrp: 0.756 ± 0.113
2.728GlyTyr: 2.728 ± 0.263
0.0GlyXaa: 0.0 ± 0.0
His
0.904HisAla: 0.904 ± 0.144
0.341HisCys: 0.341 ± 0.073
1.23HisAsp: 1.23 ± 0.147
1.186HisGlu: 1.186 ± 0.129
0.608HisPhe: 0.608 ± 0.096
1.349HisGly: 1.349 ± 0.169
0.519HisHis: 0.519 ± 0.104
1.156HisIle: 1.156 ± 0.128
1.497HisLys: 1.497 ± 0.17
1.912HisLeu: 1.912 ± 0.179
0.534HisMet: 0.534 ± 0.093
0.889HisAsn: 0.889 ± 0.119
1.156HisPro: 1.156 ± 0.125
0.637HisGln: 0.637 ± 0.105
1.127HisArg: 1.127 ± 0.118
1.216HisSer: 1.216 ± 0.127
1.008HisThr: 1.008 ± 0.119
1.156HisVal: 1.156 ± 0.125
0.222HisTrp: 0.222 ± 0.058
0.934HisTyr: 0.934 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
3.988IleAla: 3.988 ± 0.228
0.46IleCys: 0.46 ± 0.08
5.144IleAsp: 5.144 ± 0.364
5.885IleGlu: 5.885 ± 0.34
2.149IlePhe: 2.149 ± 0.193
3.958IleGly: 3.958 ± 0.282
1.571IleHis: 1.571 ± 0.168
4.254IleIle: 4.254 ± 0.333
5.233IleLys: 5.233 ± 0.297
5.262IleLeu: 5.262 ± 0.296
1.705IleMet: 1.705 ± 0.15
3.81IleAsn: 3.81 ± 0.27
3.365IlePro: 3.365 ± 0.277
2.135IleGln: 2.135 ± 0.17
3.335IleArg: 3.335 ± 0.24
4.803IleSer: 4.803 ± 0.393
3.928IleThr: 3.928 ± 0.262
4.729IleVal: 4.729 ± 0.271
0.548IleTrp: 0.548 ± 0.097
2.357IleTyr: 2.357 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
4.506LysAla: 4.506 ± 0.376
0.726LysCys: 0.726 ± 0.105
4.847LysAsp: 4.847 ± 0.255
5.5LysGlu: 5.5 ± 0.337
3.054LysPhe: 3.054 ± 0.249
4.091LysGly: 4.091 ± 0.262
1.571LysHis: 1.571 ± 0.181
4.921LysIle: 4.921 ± 0.24
4.61LysLys: 4.61 ± 0.406
7.486LysLeu: 7.486 ± 0.485
2.327LysMet: 2.327 ± 0.174
2.831LysAsn: 2.831 ± 0.223
2.164LysPro: 2.164 ± 0.187
1.838LysGln: 1.838 ± 0.18
2.683LysArg: 2.683 ± 0.261
5.944LysSer: 5.944 ± 0.391
3.246LysThr: 3.246 ± 0.279
6.122LysVal: 6.122 ± 0.347
0.726LysTrp: 0.726 ± 0.13
3.35LysTyr: 3.35 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
5.144LeuAla: 5.144 ± 0.328
0.756LeuCys: 0.756 ± 0.149
6.715LeuAsp: 6.715 ± 0.284
7.204LeuGlu: 7.204 ± 0.344
2.431LeuPhe: 2.431 ± 0.189
5.663LeuGly: 5.663 ± 0.407
1.586LeuHis: 1.586 ± 0.164
5.381LeuIle: 5.381 ± 0.315
5.796LeuLys: 5.796 ± 0.415
6.463LeuLeu: 6.463 ± 0.365
2.416LeuMet: 2.416 ± 0.208
4.403LeuAsn: 4.403 ± 0.264
3.558LeuPro: 3.558 ± 0.222
2.505LeuGln: 2.505 ± 0.206
3.943LeuArg: 3.943 ± 0.224
6.493LeuSer: 6.493 ± 0.306
4.625LeuThr: 4.625 ± 0.289
6.241LeuVal: 6.241 ± 0.336
0.712LeuTrp: 0.712 ± 0.091
3.484LeuTyr: 3.484 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
1.986MetAla: 1.986 ± 0.223
0.208MetCys: 0.208 ± 0.061
1.616MetAsp: 1.616 ± 0.162
1.749MetGlu: 1.749 ± 0.177
1.067MetPhe: 1.067 ± 0.135
1.23MetGly: 1.23 ± 0.134
0.356MetHis: 0.356 ± 0.09
1.957MetIle: 1.957 ± 0.171
2.476MetLys: 2.476 ± 0.202
1.883MetLeu: 1.883 ± 0.164
0.667MetMet: 0.667 ± 0.102
1.601MetAsn: 1.601 ± 0.164
0.934MetPro: 0.934 ± 0.118
0.637MetGln: 0.637 ± 0.1
1.082MetArg: 1.082 ± 0.149
1.838MetSer: 1.838 ± 0.189
1.794MetThr: 1.794 ± 0.151
1.482MetVal: 1.482 ± 0.133
0.222MetTrp: 0.222 ± 0.058
1.038MetTyr: 1.038 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.52AsnAla: 2.52 ± 0.23
0.489AsnCys: 0.489 ± 0.092
2.728AsnAsp: 2.728 ± 0.183
3.869AsnGlu: 3.869 ± 0.284
1.942AsnPhe: 1.942 ± 0.172
3.276AsnGly: 3.276 ± 0.244
0.919AsnHis: 0.919 ± 0.124
2.965AsnIle: 2.965 ± 0.256
3.676AsnLys: 3.676 ± 0.233
4.536AsnLeu: 4.536 ± 0.277
1.23AsnMet: 1.23 ± 0.159
2.787AsnAsn: 2.787 ± 0.239
3.083AsnPro: 3.083 ± 0.261
2.401AsnGln: 2.401 ± 0.314
2.624AsnArg: 2.624 ± 0.163
3.513AsnSer: 3.513 ± 0.233
2.639AsnThr: 2.639 ± 0.233
2.92AsnVal: 2.92 ± 0.224
0.697AsnTrp: 0.697 ± 0.099
2.06AsnTyr: 2.06 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
1.897ProAla: 1.897 ± 0.172
0.252ProCys: 0.252 ± 0.064
2.935ProAsp: 2.935 ± 0.213
3.736ProGlu: 3.736 ± 0.273
1.334ProPhe: 1.334 ± 0.144
2.268ProGly: 2.268 ± 0.372
0.712ProHis: 0.712 ± 0.107
2.105ProIle: 2.105 ± 0.18
2.787ProLys: 2.787 ± 0.22
2.253ProLeu: 2.253 ± 0.215
0.563ProMet: 0.563 ± 0.082
2.016ProAsn: 2.016 ± 0.231
1.275ProPro: 1.275 ± 0.193
1.082ProGln: 1.082 ± 0.115
1.705ProArg: 1.705 ± 0.164
2.787ProSer: 2.787 ± 0.335
2.609ProThr: 2.609 ± 0.193
2.668ProVal: 2.668 ± 0.244
0.356ProTrp: 0.356 ± 0.073
1.675ProTyr: 1.675 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
2.105GlnAla: 2.105 ± 0.194
0.237GlnCys: 0.237 ± 0.055
1.853GlnAsp: 1.853 ± 0.158
2.09GlnGlu: 2.09 ± 0.174
1.853GlnPhe: 1.853 ± 0.229
2.12GlnGly: 2.12 ± 0.288
0.519GlnHis: 0.519 ± 0.095
2.327GlnIle: 2.327 ± 0.214
1.453GlnLys: 1.453 ± 0.17
3.617GlnLeu: 3.617 ± 0.274
0.904GlnMet: 0.904 ± 0.126
1.112GlnAsn: 1.112 ± 0.161
0.993GlnPro: 0.993 ± 0.133
0.993GlnGln: 0.993 ± 0.236
1.29GlnArg: 1.29 ± 0.114
2.164GlnSer: 2.164 ± 0.175
1.408GlnThr: 1.408 ± 0.147
2.787GlnVal: 2.787 ± 0.213
0.623GlnTrp: 0.623 ± 0.108
1.616GlnTyr: 1.616 ± 0.157
0.0GlnXaa: 0.0 ± 0.0
Arg
2.253ArgAla: 2.253 ± 0.179
0.489ArgCys: 0.489 ± 0.085
3.32ArgAsp: 3.32 ± 0.243
3.543ArgGlu: 3.543 ± 0.25
1.883ArgPhe: 1.883 ± 0.197
3.143ArgGly: 3.143 ± 0.207
0.845ArgHis: 0.845 ± 0.108
3.335ArgIle: 3.335 ± 0.234
3.795ArgLys: 3.795 ± 0.401
4.417ArgLeu: 4.417 ± 0.347
1.23ArgMet: 1.23 ± 0.165
2.742ArgAsn: 2.742 ± 0.245
1.586ArgPro: 1.586 ± 0.177
1.497ArgGln: 1.497 ± 0.15
2.728ArgArg: 2.728 ± 0.229
3.009ArgSer: 3.009 ± 0.187
2.283ArgThr: 2.283 ± 0.226
3.306ArgVal: 3.306 ± 0.219
0.608ArgTrp: 0.608 ± 0.106
2.209ArgTyr: 2.209 ± 0.214
0.0ArgXaa: 0.0 ± 0.0
Ser
4.091SerAla: 4.091 ± 0.364
0.578SerCys: 0.578 ± 0.103
5.233SerAsp: 5.233 ± 0.513
5.336SerGlu: 5.336 ± 0.269
2.401SerPhe: 2.401 ± 0.211
4.669SerGly: 4.669 ± 0.348
1.379SerHis: 1.379 ± 0.147
5.603SerIle: 5.603 ± 0.617
5.485SerLys: 5.485 ± 0.339
5.707SerLeu: 5.707 ± 0.363
1.808SerMet: 1.808 ± 0.158
3.973SerAsn: 3.973 ± 0.246
2.342SerPro: 2.342 ± 0.185
2.476SerGln: 2.476 ± 0.18
3.854SerArg: 3.854 ± 0.241
6.404SerSer: 6.404 ± 0.481
4.18SerThr: 4.18 ± 0.26
4.492SerVal: 4.492 ± 0.309
0.652SerTrp: 0.652 ± 0.097
3.172SerTyr: 3.172 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
3.172ThrAla: 3.172 ± 0.241
0.415ThrCys: 0.415 ± 0.077
3.335ThrAsp: 3.335 ± 0.261
4.373ThrGlu: 4.373 ± 0.205
1.957ThrPhe: 1.957 ± 0.181
3.098ThrGly: 3.098 ± 0.253
1.319ThrHis: 1.319 ± 0.123
3.75ThrIle: 3.75 ± 0.237
4.299ThrLys: 4.299 ± 0.261
4.595ThrLeu: 4.595 ± 0.285
1.097ThrMet: 1.097 ± 0.147
2.965ThrAsn: 2.965 ± 0.214
2.639ThrPro: 2.639 ± 0.216
1.986ThrGln: 1.986 ± 0.177
2.579ThrArg: 2.579 ± 0.22
4.002ThrSer: 4.002 ± 0.27
2.846ThrThr: 2.846 ± 0.259
4.106ThrVal: 4.106 ± 0.302
0.712ThrTrp: 0.712 ± 0.117
2.224ThrTyr: 2.224 ± 0.18
0.0ThrXaa: 0.0 ± 0.0
Val
4.417ValAla: 4.417 ± 0.286
0.697ValCys: 0.697 ± 0.112
4.996ValAsp: 4.996 ± 0.291
5.574ValGlu: 5.574 ± 0.314
2.357ValPhe: 2.357 ± 0.187
4.21ValGly: 4.21 ± 0.288
1.453ValHis: 1.453 ± 0.14
4.773ValIle: 4.773 ± 0.305
4.714ValLys: 4.714 ± 0.303
5.307ValLeu: 5.307 ± 0.281
1.66ValMet: 1.66 ± 0.146
3.691ValAsn: 3.691 ± 0.238
2.772ValPro: 2.772 ± 0.217
2.194ValGln: 2.194 ± 0.218
3.899ValArg: 3.899 ± 0.252
5.381ValSer: 5.381 ± 0.325
4.062ValThr: 4.062 ± 0.244
5.455ValVal: 5.455 ± 0.355
0.637ValTrp: 0.637 ± 0.119
2.787ValTyr: 2.787 ± 0.186
0.0ValXaa: 0.0 ± 0.0
Trp
0.43TrpAla: 0.43 ± 0.088
0.119TrpCys: 0.119 ± 0.042
1.008TrpAsp: 1.008 ± 0.127
0.786TrpGlu: 0.786 ± 0.112
0.534TrpPhe: 0.534 ± 0.098
0.519TrpGly: 0.519 ± 0.069
0.178TrpHis: 0.178 ± 0.06
0.815TrpIle: 0.815 ± 0.101
0.593TrpLys: 0.593 ± 0.105
0.786TrpLeu: 0.786 ± 0.136
0.311TrpMet: 0.311 ± 0.071
0.563TrpAsn: 0.563 ± 0.106
0.015TrpPro: 0.015 ± 0.019
0.193TrpGln: 0.193 ± 0.049
0.385TrpArg: 0.385 ± 0.081
0.949TrpSer: 0.949 ± 0.131
0.889TrpThr: 0.889 ± 0.117
0.845TrpVal: 0.845 ± 0.122
0.119TrpTrp: 0.119 ± 0.04
0.43TrpTyr: 0.43 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.957TyrAla: 1.957 ± 0.202
0.371TyrCys: 0.371 ± 0.079
3.054TyrAsp: 3.054 ± 0.26
2.98TyrGlu: 2.98 ± 0.228
1.334TyrPhe: 1.334 ± 0.149
2.891TyrGly: 2.891 ± 0.234
1.008TyrHis: 1.008 ± 0.131
2.831TyrIle: 2.831 ± 0.255
3.039TyrLys: 3.039 ± 0.201
4.121TyrLeu: 4.121 ± 0.271
0.889TyrMet: 0.889 ± 0.108
2.238TyrAsn: 2.238 ± 0.179
1.808TyrPro: 1.808 ± 0.259
1.23TyrGln: 1.23 ± 0.157
2.639TyrArg: 2.639 ± 0.212
2.579TyrSer: 2.579 ± 0.205
2.268TyrThr: 2.268 ± 0.209
3.246TyrVal: 3.246 ± 0.222
0.341TyrTrp: 0.341 ± 0.065
1.69TyrTyr: 1.69 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 317 proteins (67461 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski