Mycobacterium phage Mantra
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MG21|A0A345MG21_9CAUD Uncharacterized protein OS=Mycobacterium phage Mantra OX=2283297 GN=79 PE=4 SV=1
MM1 pKa = 7.59 SGDD4 pKa = 3.12 INAEE8 pKa = 3.61 GFIRR12 pKa = 11.84 YY13 pKa = 9.51 GGDD16 pKa = 3.52 CTCGAIYY23 pKa = 9.12 TYY25 pKa = 10.64 GGHH28 pKa = 7.08 AEE30 pKa = 4.57 PGSFDD35 pKa = 4.3 PFCPDD40 pKa = 2.7 HH41 pKa = 7.27 GEE43 pKa = 4.02 AAVVATGEE51 pKa = 4.19 EE52 pKa = 4.15 AA53 pKa = 3.78
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.974
IPC2_protein 4.037
IPC_protein 3.859
Toseland 3.694
ProMoST 4.037
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.617
Solomon 3.808
Lehninger 3.77
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.795
Sillero 3.986
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A345MG16|A0A345MG16_9CAUD Uncharacterized protein OS=Mycobacterium phage Mantra OX=2283297 GN=74 PE=4 SV=1
MM1 pKa = 8.05 PDD3 pKa = 3.22 RR4 pKa = 11.84 YY5 pKa = 10.68 GDD7 pKa = 3.63 PTPEE11 pKa = 3.56 PRR13 pKa = 11.84 VFVRR17 pKa = 11.84 PKK19 pKa = 10.42 VNALTVRR26 pKa = 11.84 CSWCKK31 pKa = 9.95 AGVGSRR37 pKa = 11.84 CVVAGTGVVLRR48 pKa = 11.84 RR49 pKa = 11.84 SSFHH53 pKa = 6.57 DD54 pKa = 3.25 VRR56 pKa = 11.84 VRR58 pKa = 11.84 DD59 pKa = 3.96 AEE61 pKa = 4.18 LAATGALARR70 pKa = 11.84 GRR72 pKa = 11.84 MSS74 pKa = 3.0
Molecular weight: 8.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.502
IPC_protein 10.657
Toseland 10.818
ProMoST 10.862
Dawson 10.862
Bjellqvist 10.716
Wikipedia 11.184
Rodwell 10.804
Grimsley 10.906
Solomon 11.155
Lehninger 11.096
Nozaki 10.847
DTASelect 10.701
Thurlkill 10.818
EMBOSS 11.257
Sillero 10.833
Patrickios 10.643
IPC_peptide 11.155
IPC2_peptide 10.218
IPC2.peptide.svr19 8.879
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
102
0
102
18100
19
1210
177.5
19.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.525 ± 0.499
1.21 ± 0.187
6.486 ± 0.224
5.983 ± 0.353
2.972 ± 0.202
8.878 ± 0.602
2.204 ± 0.179
4.282 ± 0.211
3.414 ± 0.208
7.116 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.188 ± 0.145
3.37 ± 0.178
6.099 ± 0.224
3.282 ± 0.18
6.823 ± 0.416
6.094 ± 0.27
6.906 ± 0.262
7.271 ± 0.264
2.343 ± 0.14
2.552 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here