Amino acid dipepetide frequency for Mycobacterium phage Mantra

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.592AlaAla: 13.592 ± 1.874
0.995AlaCys: 0.995 ± 0.301
7.183AlaAsp: 7.183 ± 0.616
7.569AlaGlu: 7.569 ± 0.687
2.431AlaPhe: 2.431 ± 0.385
10.222AlaGly: 10.222 ± 1.395
2.321AlaHis: 2.321 ± 0.396
4.033AlaIle: 4.033 ± 0.517
4.033AlaLys: 4.033 ± 0.494
8.011AlaLeu: 8.011 ± 0.716
2.321AlaMet: 2.321 ± 0.481
2.431AlaAsn: 2.431 ± 0.487
5.636AlaPro: 5.636 ± 0.76
2.984AlaGln: 2.984 ± 0.479
6.962AlaArg: 6.962 ± 0.644
5.304AlaSer: 5.304 ± 0.643
7.183AlaThr: 7.183 ± 0.773
7.017AlaVal: 7.017 ± 0.674
2.597AlaTrp: 2.597 ± 0.364
2.431AlaTyr: 2.431 ± 0.372
0.0AlaXaa: 0.0 ± 0.0
Cys
0.718CysAla: 0.718 ± 0.232
0.0CysCys: 0.0 ± 0.0
1.105CysAsp: 1.105 ± 0.317
1.216CysGlu: 1.216 ± 0.285
0.276CysPhe: 0.276 ± 0.111
1.326CysGly: 1.326 ± 0.376
0.221CysHis: 0.221 ± 0.106
0.387CysIle: 0.387 ± 0.179
0.553CysLys: 0.553 ± 0.177
0.884CysLeu: 0.884 ± 0.297
0.166CysMet: 0.166 ± 0.13
0.387CysAsn: 0.387 ± 0.147
1.16CysPro: 1.16 ± 0.341
0.221CysGln: 0.221 ± 0.101
0.829CysArg: 0.829 ± 0.248
0.663CysSer: 0.663 ± 0.26
0.718CysThr: 0.718 ± 0.255
0.829CysVal: 0.829 ± 0.214
0.332CysTrp: 0.332 ± 0.146
0.111CysTyr: 0.111 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
6.685AspAla: 6.685 ± 0.682
0.829AspCys: 0.829 ± 0.204
4.42AspAsp: 4.42 ± 0.46
3.37AspGlu: 3.37 ± 0.431
1.492AspPhe: 1.492 ± 0.243
6.409AspGly: 6.409 ± 0.673
1.16AspHis: 1.16 ± 0.264
2.321AspIle: 2.321 ± 0.337
2.155AspLys: 2.155 ± 0.367
5.801AspLeu: 5.801 ± 0.58
1.105AspMet: 1.105 ± 0.257
1.989AspAsn: 1.989 ± 0.358
4.696AspPro: 4.696 ± 0.622
2.265AspGln: 2.265 ± 0.344
4.696AspArg: 4.696 ± 0.664
3.702AspSer: 3.702 ± 0.555
4.199AspThr: 4.199 ± 0.547
4.31AspVal: 4.31 ± 0.592
1.381AspTrp: 1.381 ± 0.272
1.879AspTyr: 1.879 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
6.575GluAla: 6.575 ± 0.68
0.939GluCys: 0.939 ± 0.285
3.149GluAsp: 3.149 ± 0.441
2.984GluGlu: 2.984 ± 0.531
2.044GluPhe: 2.044 ± 0.343
3.702GluGly: 3.702 ± 0.456
1.934GluHis: 1.934 ± 0.358
2.1GluIle: 2.1 ± 0.305
1.713GluLys: 1.713 ± 0.283
5.857GluLeu: 5.857 ± 0.679
1.547GluMet: 1.547 ± 0.316
1.823GluAsn: 1.823 ± 0.24
3.039GluPro: 3.039 ± 0.459
2.652GluGln: 2.652 ± 0.36
5.138GluArg: 5.138 ± 0.666
2.928GluSer: 2.928 ± 0.462
4.586GluThr: 4.586 ± 0.681
3.647GluVal: 3.647 ± 0.487
1.492GluTrp: 1.492 ± 0.298
1.989GluTyr: 1.989 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
3.26PheAla: 3.26 ± 0.423
0.276PheCys: 0.276 ± 0.147
2.542PheAsp: 2.542 ± 0.395
1.658PheGlu: 1.658 ± 0.364
0.995PhePhe: 0.995 ± 0.264
2.486PheGly: 2.486 ± 0.561
0.497PheHis: 0.497 ± 0.188
1.437PheIle: 1.437 ± 0.369
1.105PheLys: 1.105 ± 0.269
1.492PheLeu: 1.492 ± 0.252
0.663PheMet: 0.663 ± 0.211
1.326PheAsn: 1.326 ± 0.392
1.658PhePro: 1.658 ± 0.314
1.326PheGln: 1.326 ± 0.32
1.602PheArg: 1.602 ± 0.26
1.602PheSer: 1.602 ± 0.302
2.376PheThr: 2.376 ± 0.329
1.989PheVal: 1.989 ± 0.297
0.608PheTrp: 0.608 ± 0.164
0.829PheTyr: 0.829 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
9.338GlyAla: 9.338 ± 1.263
1.326GlyCys: 1.326 ± 0.278
5.691GlyAsp: 5.691 ± 0.63
3.978GlyGlu: 3.978 ± 0.563
2.873GlyPhe: 2.873 ± 0.462
10.829GlyGly: 10.829 ± 2.338
2.1GlyHis: 2.1 ± 0.332
3.812GlyIle: 3.812 ± 0.62
2.265GlyLys: 2.265 ± 0.435
5.47GlyLeu: 5.47 ± 0.629
2.265GlyMet: 2.265 ± 0.449
3.426GlyAsn: 3.426 ± 0.38
4.144GlyPro: 4.144 ± 0.681
2.321GlyGln: 2.321 ± 0.609
5.525GlyArg: 5.525 ± 0.666
6.962GlySer: 6.962 ± 0.919
6.409GlyThr: 6.409 ± 0.774
5.746GlyVal: 5.746 ± 0.685
2.265GlyTrp: 2.265 ± 0.317
2.044GlyTyr: 2.044 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.768HisAla: 1.768 ± 0.331
0.332HisCys: 0.332 ± 0.177
0.995HisAsp: 0.995 ± 0.231
1.381HisGlu: 1.381 ± 0.292
0.497HisPhe: 0.497 ± 0.168
1.768HisGly: 1.768 ± 0.303
0.829HisHis: 0.829 ± 0.26
1.381HisIle: 1.381 ± 0.336
0.995HisLys: 0.995 ± 0.203
1.437HisLeu: 1.437 ± 0.236
0.553HisMet: 0.553 ± 0.164
0.884HisAsn: 0.884 ± 0.24
1.271HisPro: 1.271 ± 0.228
0.718HisGln: 0.718 ± 0.234
1.713HisArg: 1.713 ± 0.293
0.829HisSer: 0.829 ± 0.236
1.768HisThr: 1.768 ± 0.346
1.602HisVal: 1.602 ± 0.333
0.553HisTrp: 0.553 ± 0.181
0.774HisTyr: 0.774 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
4.807IleAla: 4.807 ± 0.536
0.553IleCys: 0.553 ± 0.243
3.591IleAsp: 3.591 ± 0.483
3.536IleGlu: 3.536 ± 0.421
0.718IlePhe: 0.718 ± 0.224
3.812IleGly: 3.812 ± 0.559
1.437IleHis: 1.437 ± 0.329
1.437IleIle: 1.437 ± 0.28
1.381IleLys: 1.381 ± 0.261
2.321IleLeu: 2.321 ± 0.356
0.276IleMet: 0.276 ± 0.113
1.879IleAsn: 1.879 ± 0.356
2.321IlePro: 2.321 ± 0.407
1.271IleGln: 1.271 ± 0.297
2.376IleArg: 2.376 ± 0.375
2.597IleSer: 2.597 ± 0.443
3.481IleThr: 3.481 ± 0.373
3.26IleVal: 3.26 ± 0.433
1.05IleTrp: 1.05 ± 0.252
0.718IleTyr: 0.718 ± 0.169
0.0IleXaa: 0.0 ± 0.0
Lys
3.868LysAla: 3.868 ± 0.434
0.442LysCys: 0.442 ± 0.162
1.823LysAsp: 1.823 ± 0.294
1.271LysGlu: 1.271 ± 0.297
1.271LysPhe: 1.271 ± 0.215
2.321LysGly: 2.321 ± 0.362
0.995LysHis: 0.995 ± 0.271
0.829LysIle: 0.829 ± 0.219
1.492LysLys: 1.492 ± 0.437
2.431LysLeu: 2.431 ± 0.471
0.442LysMet: 0.442 ± 0.157
0.884LysAsn: 0.884 ± 0.212
2.873LysPro: 2.873 ± 0.481
1.492LysGln: 1.492 ± 0.216
2.707LysArg: 2.707 ± 0.454
2.155LysSer: 2.155 ± 0.319
2.21LysThr: 2.21 ± 0.443
2.707LysVal: 2.707 ± 0.368
0.884LysTrp: 0.884 ± 0.257
0.995LysTyr: 0.995 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
8.288LeuAla: 8.288 ± 0.952
0.608LeuCys: 0.608 ± 0.194
4.641LeuAsp: 4.641 ± 0.507
4.033LeuGlu: 4.033 ± 0.41
2.431LeuPhe: 2.431 ± 0.315
4.862LeuGly: 4.862 ± 0.551
0.829LeuHis: 0.829 ± 0.219
2.928LeuIle: 2.928 ± 0.415
2.044LeuLys: 2.044 ± 0.34
4.531LeuLeu: 4.531 ± 0.534
1.547LeuMet: 1.547 ± 0.287
2.763LeuAsn: 2.763 ± 0.477
5.304LeuPro: 5.304 ± 0.622
2.652LeuGln: 2.652 ± 0.462
5.028LeuArg: 5.028 ± 0.599
4.917LeuSer: 4.917 ± 0.568
5.249LeuThr: 5.249 ± 0.516
5.083LeuVal: 5.083 ± 0.498
0.995LeuTrp: 0.995 ± 0.218
2.431LeuTyr: 2.431 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
2.321MetAla: 2.321 ± 0.348
0.276MetCys: 0.276 ± 0.19
1.16MetAsp: 1.16 ± 0.274
0.718MetGlu: 0.718 ± 0.152
0.497MetPhe: 0.497 ± 0.15
1.713MetGly: 1.713 ± 0.257
0.111MetHis: 0.111 ± 0.08
0.939MetIle: 0.939 ± 0.252
0.995MetLys: 0.995 ± 0.237
1.658MetLeu: 1.658 ± 0.276
0.442MetMet: 0.442 ± 0.217
0.774MetAsn: 0.774 ± 0.204
1.326MetPro: 1.326 ± 0.299
0.497MetGln: 0.497 ± 0.179
1.713MetArg: 1.713 ± 0.3
2.707MetSer: 2.707 ± 0.38
1.934MetThr: 1.934 ± 0.332
1.381MetVal: 1.381 ± 0.321
0.387MetTrp: 0.387 ± 0.158
0.332MetTyr: 0.332 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.591AsnAla: 3.591 ± 0.477
0.387AsnCys: 0.387 ± 0.137
1.713AsnAsp: 1.713 ± 0.277
1.989AsnGlu: 1.989 ± 0.371
0.939AsnPhe: 0.939 ± 0.315
3.868AsnGly: 3.868 ± 0.582
1.05AsnHis: 1.05 ± 0.26
1.658AsnIle: 1.658 ± 0.416
1.216AsnLys: 1.216 ± 0.311
2.376AsnLeu: 2.376 ± 0.37
0.663AsnMet: 0.663 ± 0.165
1.768AsnAsn: 1.768 ± 0.327
2.597AsnPro: 2.597 ± 0.356
1.05AsnGln: 1.05 ± 0.355
1.879AsnArg: 1.879 ± 0.406
1.713AsnSer: 1.713 ± 0.323
2.044AsnThr: 2.044 ± 0.288
1.768AsnVal: 1.768 ± 0.299
0.829AsnTrp: 0.829 ± 0.234
0.608AsnTyr: 0.608 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
5.415ProAla: 5.415 ± 0.701
0.442ProCys: 0.442 ± 0.181
4.42ProAsp: 4.42 ± 0.417
4.862ProGlu: 4.862 ± 0.539
1.547ProPhe: 1.547 ± 0.329
6.299ProGly: 6.299 ± 0.758
1.381ProHis: 1.381 ± 0.267
1.823ProIle: 1.823 ± 0.344
2.155ProLys: 2.155 ± 0.382
4.144ProLeu: 4.144 ± 0.512
1.381ProMet: 1.381 ± 0.29
1.989ProAsn: 1.989 ± 0.288
3.978ProPro: 3.978 ± 0.65
2.155ProGln: 2.155 ± 0.381
3.149ProArg: 3.149 ± 0.504
3.812ProSer: 3.812 ± 0.536
3.812ProThr: 3.812 ± 0.537
4.973ProVal: 4.973 ± 0.583
1.437ProTrp: 1.437 ± 0.238
1.823ProTyr: 1.823 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
3.978GlnAla: 3.978 ± 0.627
0.332GlnCys: 0.332 ± 0.192
1.271GlnAsp: 1.271 ± 0.248
1.658GlnGlu: 1.658 ± 0.4
1.105GlnPhe: 1.105 ± 0.256
2.652GlnGly: 2.652 ± 0.428
1.105GlnHis: 1.105 ± 0.289
1.713GlnIle: 1.713 ± 0.319
1.105GlnLys: 1.105 ± 0.24
2.321GlnLeu: 2.321 ± 0.38
0.663GlnMet: 0.663 ± 0.196
0.884GlnAsn: 0.884 ± 0.216
2.597GlnPro: 2.597 ± 0.457
1.05GlnGln: 1.05 ± 0.29
2.818GlnArg: 2.818 ± 0.366
1.989GlnSer: 1.989 ± 0.315
1.658GlnThr: 1.658 ± 0.382
2.265GlnVal: 2.265 ± 0.358
0.774GlnTrp: 0.774 ± 0.209
0.884GlnTyr: 0.884 ± 0.264
0.0GlnXaa: 0.0 ± 0.0
Arg
6.575ArgAla: 6.575 ± 0.665
1.105ArgCys: 1.105 ± 0.297
4.696ArgAsp: 4.696 ± 0.609
4.696ArgGlu: 4.696 ± 0.597
1.879ArgPhe: 1.879 ± 0.341
4.199ArgGly: 4.199 ± 0.41
1.105ArgHis: 1.105 ± 0.233
4.033ArgIle: 4.033 ± 0.649
2.542ArgLys: 2.542 ± 0.413
5.083ArgLeu: 5.083 ± 0.546
2.818ArgMet: 2.818 ± 0.413
2.265ArgAsn: 2.265 ± 0.456
3.812ArgPro: 3.812 ± 0.475
1.658ArgGln: 1.658 ± 0.271
5.138ArgArg: 5.138 ± 0.79
3.757ArgSer: 3.757 ± 0.427
3.536ArgThr: 3.536 ± 0.536
5.138ArgVal: 5.138 ± 0.543
2.044ArgTrp: 2.044 ± 0.357
2.155ArgTyr: 2.155 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
5.47SerAla: 5.47 ± 0.753
0.608SerCys: 0.608 ± 0.252
3.868SerAsp: 3.868 ± 0.489
3.205SerGlu: 3.205 ± 0.4
2.265SerPhe: 2.265 ± 0.468
6.906SerGly: 6.906 ± 0.832
1.05SerHis: 1.05 ± 0.199
3.094SerIle: 3.094 ± 0.44
2.763SerLys: 2.763 ± 0.425
3.868SerLeu: 3.868 ± 0.461
1.381SerMet: 1.381 ± 0.268
2.265SerAsn: 2.265 ± 0.515
3.26SerPro: 3.26 ± 0.351
1.823SerGln: 1.823 ± 0.291
3.591SerArg: 3.591 ± 0.414
4.641SerSer: 4.641 ± 0.7
3.978SerThr: 3.978 ± 0.469
3.978SerVal: 3.978 ± 0.547
1.381SerTrp: 1.381 ± 0.28
1.547SerTyr: 1.547 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
7.072ThrAla: 7.072 ± 0.664
0.718ThrCys: 0.718 ± 0.223
3.923ThrAsp: 3.923 ± 0.634
4.254ThrGlu: 4.254 ± 0.532
2.321ThrPhe: 2.321 ± 0.371
6.575ThrGly: 6.575 ± 0.685
1.547ThrHis: 1.547 ± 0.281
3.923ThrIle: 3.923 ± 0.465
1.823ThrLys: 1.823 ± 0.366
4.254ThrLeu: 4.254 ± 0.496
1.271ThrMet: 1.271 ± 0.259
2.21ThrAsn: 2.21 ± 0.341
4.862ThrPro: 4.862 ± 0.551
1.823ThrGln: 1.823 ± 0.266
4.475ThrArg: 4.475 ± 0.503
3.812ThrSer: 3.812 ± 0.524
4.862ThrThr: 4.862 ± 0.684
6.464ThrVal: 6.464 ± 0.607
1.16ThrTrp: 1.16 ± 0.334
1.713ThrTyr: 1.713 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
7.569ValAla: 7.569 ± 0.601
1.326ValCys: 1.326 ± 0.291
5.746ValAsp: 5.746 ± 0.63
4.365ValGlu: 4.365 ± 0.504
2.597ValPhe: 2.597 ± 0.404
5.636ValGly: 5.636 ± 0.676
1.271ValHis: 1.271 ± 0.32
2.707ValIle: 2.707 ± 0.439
2.486ValLys: 2.486 ± 0.385
5.359ValLeu: 5.359 ± 0.579
1.216ValMet: 1.216 ± 0.257
2.265ValAsn: 2.265 ± 0.41
4.199ValPro: 4.199 ± 0.495
2.707ValGln: 2.707 ± 0.379
4.254ValArg: 4.254 ± 0.566
4.42ValSer: 4.42 ± 0.539
5.415ValThr: 5.415 ± 0.557
6.354ValVal: 6.354 ± 0.726
1.602ValTrp: 1.602 ± 0.374
1.216ValTyr: 1.216 ± 0.246
0.0ValXaa: 0.0 ± 0.0
Trp
2.044TrpAla: 2.044 ± 0.317
0.332TrpCys: 0.332 ± 0.134
1.16TrpAsp: 1.16 ± 0.252
1.216TrpGlu: 1.216 ± 0.323
0.774TrpPhe: 0.774 ± 0.203
1.105TrpGly: 1.105 ± 0.26
0.718TrpHis: 0.718 ± 0.206
1.05TrpIle: 1.05 ± 0.27
0.829TrpLys: 0.829 ± 0.183
1.823TrpLeu: 1.823 ± 0.355
0.939TrpMet: 0.939 ± 0.259
0.663TrpAsn: 0.663 ± 0.223
0.995TrpPro: 0.995 ± 0.234
1.05TrpGln: 1.05 ± 0.228
2.542TrpArg: 2.542 ± 0.482
1.492TrpSer: 1.492 ± 0.343
1.547TrpThr: 1.547 ± 0.28
1.989TrpVal: 1.989 ± 0.445
0.829TrpTrp: 0.829 ± 0.214
0.332TrpTyr: 0.332 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.321TyrAla: 2.321 ± 0.391
0.276TyrCys: 0.276 ± 0.118
1.768TyrAsp: 1.768 ± 0.339
1.879TyrGlu: 1.879 ± 0.314
0.774TyrPhe: 0.774 ± 0.169
2.1TyrGly: 2.1 ± 0.34
0.387TyrHis: 0.387 ± 0.128
1.216TyrIle: 1.216 ± 0.228
0.497TyrLys: 0.497 ± 0.183
2.044TyrLeu: 2.044 ± 0.342
0.166TyrMet: 0.166 ± 0.087
0.829TyrAsn: 0.829 ± 0.209
1.271TyrPro: 1.271 ± 0.243
1.105TyrGln: 1.105 ± 0.221
2.1TyrArg: 2.1 ± 0.383
0.939TyrSer: 0.939 ± 0.219
2.1TyrThr: 2.1 ± 0.394
2.21TyrVal: 2.21 ± 0.341
0.829TyrTrp: 0.829 ± 0.216
0.718TyrTyr: 0.718 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (18100 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski