bacterium D16-34

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1706 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9AYX7|A0A3A9AYX7_9BACT ABC transporter permease OS=bacterium D16-34 OX=2320120 GN=D7W09_03945 PE=3 SV=1
MM1 pKa = 7.73DD2 pKa = 4.08QQPQNLNYY10 pKa = 10.33AASQPVKK17 pKa = 10.53ASSTSGMAITSLVLGILAIVSSWMPIVNNFSMVLAVLGVVFAIIGLVGIRR67 pKa = 11.84KK68 pKa = 9.15GKK70 pKa = 10.57KK71 pKa = 9.08KK72 pKa = 10.71GQGLAIAGLVLGILSGIIVLATQGLYY98 pKa = 10.48GAAIDD103 pKa = 4.32AARR106 pKa = 11.84EE107 pKa = 4.02EE108 pKa = 4.62INPSIAATSNVATSDD123 pKa = 3.61NASSDD128 pKa = 3.73TNGDD132 pKa = 3.85SNSDD136 pKa = 3.49SSSDD140 pKa = 3.43SSADD144 pKa = 3.41NAQEE148 pKa = 3.91EE149 pKa = 4.55APDD152 pKa = 3.85YY153 pKa = 11.49SNLSLGEE160 pKa = 4.36SIEE163 pKa = 4.11LTDD166 pKa = 4.36GSVITVNSVEE176 pKa = 4.38SGLTNYY182 pKa = 10.41DD183 pKa = 3.73DD184 pKa = 4.59SPVTGVSVTYY194 pKa = 9.76TNNGSKK200 pKa = 10.05EE201 pKa = 4.13VSFNPYY207 pKa = 9.04DD208 pKa = 3.54WKK210 pKa = 11.38AQDD213 pKa = 3.54AQGAQYY219 pKa = 10.31SQGYY223 pKa = 9.17YY224 pKa = 10.23SEE226 pKa = 5.15ADD228 pKa = 3.29NALSSGTLAPGGTVTGNVYY247 pKa = 10.55FDD249 pKa = 3.73GDD251 pKa = 3.62ITKK254 pKa = 10.17VLYY257 pKa = 9.6YY258 pKa = 11.32SNMFNDD264 pKa = 3.56SATAGWVVEE273 pKa = 4.28

Molecular weight: 28.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9B1X4|A0A3A9B1X4_9BACT ABC transporter permease OS=bacterium D16-34 OX=2320120 GN=D7W09_02135 PE=4 SV=1
MM1 pKa = 7.64LVRR4 pKa = 11.84LSRR7 pKa = 11.84IIPFLLVLAVLAAIIYY23 pKa = 10.19LVMTYY28 pKa = 9.51RR29 pKa = 11.84HH30 pKa = 5.99SPTRR34 pKa = 11.84AKK36 pKa = 10.4EE37 pKa = 3.66ILIKK41 pKa = 10.66LFIGITSVTSVFFALMSLYY60 pKa = 10.87AWFEE64 pKa = 4.3HH65 pKa = 6.24NDD67 pKa = 3.37AVFDD71 pKa = 4.13LAFSFLLTMLICLGVTLWCRR91 pKa = 11.84AVFLKK96 pKa = 10.2HH97 pKa = 5.84HH98 pKa = 6.49PEE100 pKa = 3.95YY101 pKa = 10.36RR102 pKa = 11.84KK103 pKa = 10.02KK104 pKa = 9.37PQRR107 pKa = 11.84AKK109 pKa = 10.55RR110 pKa = 11.84LNSRR114 pKa = 11.84GGRR117 pKa = 11.84KK118 pKa = 8.87

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1706

0

1706

630596

22

9993

369.6

40.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.496 ± 0.085

1.556 ± 0.04

6.238 ± 0.091

6.478 ± 0.057

3.88 ± 0.047

7.541 ± 0.06

1.866 ± 0.023

5.85 ± 0.063

4.157 ± 0.044

9.169 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.037

3.582 ± 0.069

4.214 ± 0.047

3.565 ± 0.034

4.772 ± 0.055

6.174 ± 0.039

5.845 ± 0.083

7.828 ± 0.057

1.067 ± 0.024

3.118 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski