Amino acid dipepetide frequency for bacterium D16-34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.752AlaAla: 11.752 ± 0.206
2.035AlaCys: 2.035 ± 0.075
6.281AlaAsp: 6.281 ± 0.121
6.021AlaGlu: 6.021 ± 0.115
4.18AlaPhe: 4.18 ± 0.091
7.712AlaGly: 7.712 ± 0.131
2.141AlaHis: 2.141 ± 0.066
5.998AlaIle: 5.998 ± 0.135
4.442AlaLys: 4.442 ± 0.101
10.986AlaLeu: 10.986 ± 0.219
2.625AlaMet: 2.625 ± 0.077
3.476AlaAsn: 3.476 ± 0.084
3.823AlaPro: 3.823 ± 0.116
4.918AlaGln: 4.918 ± 0.116
4.989AlaArg: 4.989 ± 0.116
6.432AlaSer: 6.432 ± 0.1
5.807AlaThr: 5.807 ± 0.112
7.058AlaVal: 7.058 ± 0.118
1.173AlaTrp: 1.173 ± 0.048
3.113AlaTyr: 3.113 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
1.898CysAla: 1.898 ± 0.066
0.251CysCys: 0.251 ± 0.019
0.955CysAsp: 0.955 ± 0.044
0.983CysGlu: 0.983 ± 0.047
0.62CysPhe: 0.62 ± 0.033
1.538CysGly: 1.538 ± 0.057
0.317CysHis: 0.317 ± 0.034
0.901CysIle: 0.901 ± 0.039
0.557CysLys: 0.557 ± 0.033
1.273CysLeu: 1.273 ± 0.05
0.408CysMet: 0.408 ± 0.029
0.446CysAsn: 0.446 ± 0.026
0.747CysPro: 0.747 ± 0.046
0.39CysGln: 0.39 ± 0.025
0.553CysArg: 0.553 ± 0.031
0.861CysSer: 0.861 ± 0.041
0.826CysThr: 0.826 ± 0.034
1.456CysVal: 1.456 ± 0.058
0.14CysTrp: 0.14 ± 0.016
0.444CysTyr: 0.444 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
7.012AspAla: 7.012 ± 0.124
0.739AspCys: 0.739 ± 0.041
3.939AspAsp: 3.939 ± 0.108
5.5AspGlu: 5.5 ± 0.108
2.298AspPhe: 2.298 ± 0.069
5.063AspGly: 5.063 ± 0.171
0.975AspHis: 0.975 ± 0.039
3.708AspIle: 3.708 ± 0.078
2.58AspLys: 2.58 ± 0.066
5.076AspLeu: 5.076 ± 0.08
1.668AspMet: 1.668 ± 0.056
2.036AspAsn: 2.036 ± 0.08
3.286AspPro: 3.286 ± 0.138
2.03AspGln: 2.03 ± 0.052
2.494AspArg: 2.494 ± 0.073
3.059AspSer: 3.059 ± 0.078
3.357AspThr: 3.357 ± 0.096
4.841AspVal: 4.841 ± 0.089
0.691AspTrp: 0.691 ± 0.041
2.027AspTyr: 2.027 ± 0.079
0.0AspXaa: 0.0 ± 0.0
Glu
6.927GluAla: 6.927 ± 0.122
0.796GluCys: 0.796 ± 0.035
3.825GluAsp: 3.825 ± 0.097
4.546GluGlu: 4.546 ± 0.107
2.085GluPhe: 2.085 ± 0.063
4.643GluGly: 4.643 ± 0.118
1.441GluHis: 1.441 ± 0.057
3.823GluIle: 3.823 ± 0.11
2.843GluLys: 2.843 ± 0.083
6.161GluLeu: 6.161 ± 0.109
1.708GluMet: 1.708 ± 0.059
2.509GluAsn: 2.509 ± 0.071
2.507GluPro: 2.507 ± 0.073
3.08GluGln: 3.08 ± 0.082
3.728GluArg: 3.728 ± 0.086
3.413GluSer: 3.413 ± 0.071
3.414GluThr: 3.414 ± 0.071
4.938GluVal: 4.938 ± 0.096
0.572GluTrp: 0.572 ± 0.03
1.816GluTyr: 1.816 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
4.42PheAla: 4.42 ± 0.089
0.668PheCys: 0.668 ± 0.036
3.135PheAsp: 3.135 ± 0.073
2.586PheGlu: 2.586 ± 0.066
1.519PhePhe: 1.519 ± 0.068
3.538PheGly: 3.538 ± 0.084
0.465PheHis: 0.465 ± 0.028
2.158PheIle: 2.158 ± 0.072
1.369PheLys: 1.369 ± 0.05
3.197PheLeu: 3.197 ± 0.106
1.061PheMet: 1.061 ± 0.041
1.335PheAsn: 1.335 ± 0.05
1.299PhePro: 1.299 ± 0.05
0.842PheGln: 0.842 ± 0.033
1.367PheArg: 1.367 ± 0.044
2.617PheSer: 2.617 ± 0.074
2.136PheThr: 2.136 ± 0.06
3.443PheVal: 3.443 ± 0.091
0.495PheTrp: 0.495 ± 0.031
1.147PheTyr: 1.147 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
7.339GlyAla: 7.339 ± 0.128
1.396GlyCys: 1.396 ± 0.054
4.053GlyAsp: 4.053 ± 0.103
4.761GlyGlu: 4.761 ± 0.104
3.343GlyPhe: 3.343 ± 0.077
5.633GlyGly: 5.633 ± 0.145
1.327GlyHis: 1.327 ± 0.049
5.071GlyIle: 5.071 ± 0.107
3.654GlyLys: 3.654 ± 0.09
6.456GlyLeu: 6.456 ± 0.117
2.211GlyMet: 2.211 ± 0.066
2.713GlyAsn: 2.713 ± 0.103
1.849GlyPro: 1.849 ± 0.059
2.363GlyGln: 2.363 ± 0.075
3.262GlyArg: 3.262 ± 0.069
5.003GlySer: 5.003 ± 0.107
4.507GlyThr: 4.507 ± 0.105
6.616GlyVal: 6.616 ± 0.109
1.059GlyTrp: 1.059 ± 0.065
2.796GlyTyr: 2.796 ± 0.089
0.0GlyXaa: 0.0 ± 0.0
His
1.957HisAla: 1.957 ± 0.054
0.301HisCys: 0.301 ± 0.023
1.243HisAsp: 1.243 ± 0.042
1.24HisGlu: 1.24 ± 0.044
0.661HisPhe: 0.661 ± 0.033
1.419HisGly: 1.419 ± 0.048
0.428HisHis: 0.428 ± 0.026
1.207HisIle: 1.207 ± 0.047
0.734HisLys: 0.734 ± 0.037
1.608HisLeu: 1.608 ± 0.048
0.512HisMet: 0.512 ± 0.03
0.683HisAsn: 0.683 ± 0.035
1.015HisPro: 1.015 ± 0.043
0.528HisGln: 0.528 ± 0.028
0.836HisArg: 0.836 ± 0.038
0.948HisSer: 0.948 ± 0.038
1.115HisThr: 1.115 ± 0.044
1.451HisVal: 1.451 ± 0.05
0.17HisTrp: 0.17 ± 0.019
0.601HisTyr: 0.601 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.635IleAla: 6.635 ± 0.132
1.058IleCys: 1.058 ± 0.048
4.091IleAsp: 4.091 ± 0.084
4.142IleGlu: 4.142 ± 0.098
2.047IlePhe: 2.047 ± 0.081
4.539IleGly: 4.539 ± 0.108
0.869IleHis: 0.869 ± 0.038
3.381IleIle: 3.381 ± 0.092
2.382IleLys: 2.382 ± 0.074
4.451IleLeu: 4.451 ± 0.112
1.567IleMet: 1.567 ± 0.056
2.231IleAsn: 2.231 ± 0.067
2.688IlePro: 2.688 ± 0.073
1.505IleGln: 1.505 ± 0.048
2.54IleArg: 2.54 ± 0.062
3.798IleSer: 3.798 ± 0.088
3.684IleThr: 3.684 ± 0.085
4.857IleVal: 4.857 ± 0.104
0.465IleTrp: 0.465 ± 0.026
1.568IleTyr: 1.568 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.107LysAla: 4.107 ± 0.09
0.438LysCys: 0.438 ± 0.028
2.61LysAsp: 2.61 ± 0.062
2.685LysGlu: 2.685 ± 0.078
1.075LysPhe: 1.075 ± 0.038
2.659LysGly: 2.659 ± 0.068
0.925LysHis: 0.925 ± 0.038
2.084LysIle: 2.084 ± 0.059
2.376LysLys: 2.376 ± 0.075
3.86LysLeu: 3.86 ± 0.092
1.088LysMet: 1.088 ± 0.039
1.613LysAsn: 1.613 ± 0.055
2.087LysPro: 2.087 ± 0.065
1.8LysGln: 1.8 ± 0.067
2.848LysArg: 2.848 ± 0.086
2.402LysSer: 2.402 ± 0.066
2.786LysThr: 2.786 ± 0.075
2.683LysVal: 2.683 ± 0.064
0.354LysTrp: 0.354 ± 0.025
1.089LysTyr: 1.089 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
10.07LeuAla: 10.07 ± 0.181
1.481LeuCys: 1.481 ± 0.057
5.839LeuAsp: 5.839 ± 0.106
5.66LeuGlu: 5.66 ± 0.13
3.728LeuPhe: 3.728 ± 0.118
6.908LeuGly: 6.908 ± 0.15
1.616LeuHis: 1.616 ± 0.054
5.213LeuIle: 5.213 ± 0.127
3.44LeuLys: 3.44 ± 0.072
8.318LeuLeu: 8.318 ± 0.18
2.485LeuMet: 2.485 ± 0.078
2.942LeuAsn: 2.942 ± 0.078
4.106LeuPro: 4.106 ± 0.089
2.648LeuGln: 2.648 ± 0.072
4.466LeuArg: 4.466 ± 0.094
6.695LeuSer: 6.695 ± 0.127
5.176LeuThr: 5.176 ± 0.102
7.475LeuVal: 7.475 ± 0.138
0.867LeuTrp: 0.867 ± 0.039
2.555LeuTyr: 2.555 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
2.655MetAla: 2.655 ± 0.076
0.366MetCys: 0.366 ± 0.028
1.438MetAsp: 1.438 ± 0.052
1.311MetGlu: 1.311 ± 0.048
0.86MetPhe: 0.86 ± 0.041
1.968MetGly: 1.968 ± 0.065
0.506MetHis: 0.506 ± 0.028
1.381MetIle: 1.381 ± 0.048
1.162MetLys: 1.162 ± 0.048
2.612MetLeu: 2.612 ± 0.079
0.672MetMet: 0.672 ± 0.035
1.01MetAsn: 1.01 ± 0.041
1.365MetPro: 1.365 ± 0.042
1.013MetGln: 1.013 ± 0.048
1.511MetArg: 1.511 ± 0.056
1.851MetSer: 1.851 ± 0.057
1.586MetThr: 1.586 ± 0.049
1.928MetVal: 1.928 ± 0.059
0.247MetTrp: 0.247 ± 0.017
0.603MetTyr: 0.603 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.873AsnAla: 3.873 ± 0.093
0.425AsnCys: 0.425 ± 0.027
2.05AsnAsp: 2.05 ± 0.084
2.29AsnGlu: 2.29 ± 0.062
1.216AsnPhe: 1.216 ± 0.05
2.626AsnGly: 2.626 ± 0.1
0.672AsnHis: 0.672 ± 0.034
2.079AsnIle: 2.079 ± 0.062
1.529AsnLys: 1.529 ± 0.06
2.919AsnLeu: 2.919 ± 0.066
0.983AsnMet: 0.983 ± 0.043
1.564AsnAsn: 1.564 ± 0.081
2.377AsnPro: 2.377 ± 0.101
1.229AsnGln: 1.229 ± 0.053
1.611AsnArg: 1.611 ± 0.052
1.97AsnSer: 1.97 ± 0.066
2.284AsnThr: 2.284 ± 0.076
2.494AsnVal: 2.494 ± 0.063
0.398AsnTrp: 0.398 ± 0.028
1.229AsnTyr: 1.229 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
4.177ProAla: 4.177 ± 0.107
0.553ProCys: 0.553 ± 0.034
3.232ProAsp: 3.232 ± 0.132
3.362ProGlu: 3.362 ± 0.094
1.779ProPhe: 1.779 ± 0.054
2.95ProGly: 2.95 ± 0.084
0.948ProHis: 0.948 ± 0.042
2.25ProIle: 2.25 ± 0.062
1.598ProLys: 1.598 ± 0.053
3.931ProLeu: 3.931 ± 0.087
0.823ProMet: 0.823 ± 0.039
1.364ProAsn: 1.364 ± 0.061
1.097ProPro: 1.097 ± 0.044
1.809ProGln: 1.809 ± 0.066
1.69ProArg: 1.69 ± 0.047
2.529ProSer: 2.529 ± 0.066
2.791ProThr: 2.791 ± 0.087
3.337ProVal: 3.337 ± 0.079
0.46ProTrp: 0.46 ± 0.024
1.454ProTyr: 1.454 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
4.334GlnAla: 4.334 ± 0.102
0.384GlnCys: 0.384 ± 0.026
1.97GlnAsp: 1.97 ± 0.059
2.179GlnGlu: 2.179 ± 0.069
1.17GlnPhe: 1.17 ± 0.039
2.369GlnGly: 2.369 ± 0.064
0.647GlnHis: 0.647 ± 0.034
2.044GlnIle: 2.044 ± 0.068
1.622GlnLys: 1.622 ± 0.059
3.213GlnLeu: 3.213 ± 0.088
0.92GlnMet: 0.92 ± 0.041
1.514GlnAsn: 1.514 ± 0.063
1.575GlnPro: 1.575 ± 0.071
1.545GlnGln: 1.545 ± 0.067
1.811GlnArg: 1.811 ± 0.06
2.089GlnSer: 2.089 ± 0.059
2.556GlnThr: 2.556 ± 0.079
2.422GlnVal: 2.422 ± 0.065
0.355GlnTrp: 0.355 ± 0.03
0.931GlnTyr: 0.931 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
4.627ArgAla: 4.627 ± 0.108
0.672ArgCys: 0.672 ± 0.037
2.734ArgAsp: 2.734 ± 0.072
3.449ArgGlu: 3.449 ± 0.087
2.122ArgPhe: 2.122 ± 0.057
3.024ArgGly: 3.024 ± 0.075
0.89ArgHis: 0.89 ± 0.038
3.178ArgIle: 3.178 ± 0.081
2.234ArgLys: 2.234 ± 0.076
4.206ArgLeu: 4.206 ± 0.107
1.495ArgMet: 1.495 ± 0.055
1.749ArgAsn: 1.749 ± 0.055
1.732ArgPro: 1.732 ± 0.05
1.757ArgGln: 1.757 ± 0.061
2.704ArgArg: 2.704 ± 0.089
2.632ArgSer: 2.632 ± 0.066
2.59ArgThr: 2.59 ± 0.075
3.779ArgVal: 3.779 ± 0.096
0.425ArgTrp: 0.425 ± 0.029
1.725ArgTyr: 1.725 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
6.166SerAla: 6.166 ± 0.103
0.945SerCys: 0.945 ± 0.046
3.538SerAsp: 3.538 ± 0.098
3.597SerGlu: 3.597 ± 0.079
2.715SerPhe: 2.715 ± 0.067
5.04SerGly: 5.04 ± 0.121
1.197SerHis: 1.197 ± 0.043
3.354SerIle: 3.354 ± 0.071
2.423SerLys: 2.423 ± 0.072
5.94SerLeu: 5.94 ± 0.107
1.659SerMet: 1.659 ± 0.057
2.134SerAsn: 2.134 ± 0.06
2.379SerPro: 2.379 ± 0.062
2.353SerGln: 2.353 ± 0.062
2.886SerArg: 2.886 ± 0.066
4.272SerSer: 4.272 ± 0.114
3.429SerThr: 3.429 ± 0.093
4.895SerVal: 4.895 ± 0.088
0.79SerTrp: 0.79 ± 0.039
2.031SerTyr: 2.031 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
4.906ThrAla: 4.906 ± 0.091
0.893ThrCys: 0.893 ± 0.041
3.646ThrAsp: 3.646 ± 0.105
3.059ThrGlu: 3.059 ± 0.083
2.269ThrPhe: 2.269 ± 0.062
4.721ThrGly: 4.721 ± 0.131
1.235ThrHis: 1.235 ± 0.04
3.658ThrIle: 3.658 ± 0.094
2.266ThrLys: 2.266 ± 0.066
5.817ThrLeu: 5.817 ± 0.108
1.221ThrMet: 1.221 ± 0.041
2.225ThrAsn: 2.225 ± 0.093
3.386ThrPro: 3.386 ± 0.086
2.123ThrGln: 2.123 ± 0.069
2.496ThrArg: 2.496 ± 0.063
3.793ThrSer: 3.793 ± 0.109
3.838ThrThr: 3.838 ± 0.135
4.164ThrVal: 4.164 ± 0.092
0.685ThrTrp: 0.685 ± 0.035
2.05ThrTyr: 2.05 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
8.037ValAla: 8.037 ± 0.151
1.529ValCys: 1.529 ± 0.058
4.886ValAsp: 4.886 ± 0.096
4.856ValGlu: 4.856 ± 0.104
3.24ValPhe: 3.24 ± 0.085
5.814ValGly: 5.814 ± 0.119
1.297ValHis: 1.297 ± 0.047
4.778ValIle: 4.778 ± 0.096
2.812ValLys: 2.812 ± 0.066
7.585ValLeu: 7.585 ± 0.146
1.912ValMet: 1.912 ± 0.059
2.639ValAsn: 2.639 ± 0.064
3.154ValPro: 3.154 ± 0.074
2.204ValGln: 2.204 ± 0.056
3.796ValArg: 3.796 ± 0.084
5.162ValSer: 5.162 ± 0.101
4.307ValThr: 4.307 ± 0.118
7.301ValVal: 7.301 ± 0.148
0.818ValTrp: 0.818 ± 0.044
2.152ValTyr: 2.152 ± 0.07
0.0ValXaa: 0.0 ± 0.0
Trp
0.948TrpAla: 0.948 ± 0.041
0.17TrpCys: 0.17 ± 0.018
0.633TrpAsp: 0.633 ± 0.034
0.565TrpGlu: 0.565 ± 0.03
0.457TrpPhe: 0.457 ± 0.027
0.825TrpGly: 0.825 ± 0.037
0.212TrpHis: 0.212 ± 0.016
0.653TrpIle: 0.653 ± 0.033
0.415TrpLys: 0.415 ± 0.024
1.069TrpLeu: 1.069 ± 0.046
0.343TrpMet: 0.343 ± 0.022
0.427TrpAsn: 0.427 ± 0.036
0.362TrpPro: 0.362 ± 0.026
0.452TrpGln: 0.452 ± 0.03
0.557TrpArg: 0.557 ± 0.034
0.561TrpSer: 0.561 ± 0.033
0.517TrpThr: 0.517 ± 0.041
0.831TrpVal: 0.831 ± 0.042
0.144TrpTrp: 0.144 ± 0.015
0.53TrpTyr: 0.53 ± 0.054
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.122TyrAla: 3.122 ± 0.072
0.465TyrCys: 0.465 ± 0.029
2.282TyrAsp: 2.282 ± 0.091
1.985TyrGlu: 1.985 ± 0.063
1.41TyrPhe: 1.41 ± 0.06
2.423TyrGly: 2.423 ± 0.07
0.549TyrHis: 0.549 ± 0.028
1.579TyrIle: 1.579 ± 0.058
1.131TyrLys: 1.131 ± 0.05
3.01TyrLeu: 3.01 ± 0.078
0.685TyrMet: 0.685 ± 0.032
1.208TyrAsn: 1.208 ± 0.056
1.304TyrPro: 1.304 ± 0.048
1.059TyrGln: 1.059 ± 0.041
1.578TyrArg: 1.578 ± 0.055
1.656TyrSer: 1.656 ± 0.06
1.748TyrThr: 1.748 ± 0.077
2.307TyrVal: 2.307 ± 0.069
0.36TyrTrp: 0.36 ± 0.028
1.318TyrTyr: 1.318 ± 0.078
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1706 proteins (630596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski