SAR202 cluster bacterium AD-802-K11_MRT_200m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YSB8|A0A5N8YSB8_9CHLR SDR family oxidoreductase OS=SAR202 cluster bacterium AD-802-K11_MRT_200m OX=2587838 GN=FIM05_03695 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.07EE3 pKa = 4.35SIWIEE8 pKa = 3.85TEE10 pKa = 4.07EE11 pKa = 4.21NDD13 pKa = 3.33NCEE16 pKa = 3.88WMPLQVYY23 pKa = 7.65EE24 pKa = 4.08AHH26 pKa = 6.04QPPVPVKK33 pKa = 10.32LVYY36 pKa = 10.91LEE38 pKa = 5.49DD39 pKa = 4.63PDD41 pKa = 5.11RR42 pKa = 11.84DD43 pKa = 3.44PGMYY47 pKa = 9.87EE48 pKa = 3.66VTGWSSEE55 pKa = 4.06DD56 pKa = 3.42SGSICPALYY65 pKa = 10.67APVSDD70 pKa = 3.66SGQAVVHH77 pKa = 5.93LVYY80 pKa = 10.92GGDD83 pKa = 2.65WGIRR87 pKa = 11.84LRR89 pKa = 11.84PEE91 pKa = 3.85NSQGPWSLDD100 pKa = 2.91AMDD103 pKa = 3.88QHH105 pKa = 6.97GEE107 pKa = 4.1PYY109 pKa = 11.11LMLIDD114 pKa = 4.4GSDD117 pKa = 3.78VIKK120 pKa = 10.81DD121 pKa = 3.43

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YRY6|A0A5N8YRY6_9CHLR PMT_2 domain-containing protein OS=SAR202 cluster bacterium AD-802-K11_MRT_200m OX=2587838 GN=FIM05_00265 PE=4 SV=1
MM1 pKa = 7.34VNRR4 pKa = 11.84NSSRR8 pKa = 11.84IGRR11 pKa = 11.84RR12 pKa = 11.84QGVPPRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LIRR23 pKa = 11.84LEE25 pKa = 3.87VGIPSVPVRR34 pKa = 11.84NSQHH38 pKa = 6.81GITEE42 pKa = 4.47DD43 pKa = 3.28GLYY46 pKa = 10.15VISVAARR53 pKa = 11.84LLEE56 pKa = 4.04MHH58 pKa = 6.87PQTLRR63 pKa = 11.84KK64 pKa = 9.39YY65 pKa = 9.09EE66 pKa = 3.92RR67 pKa = 11.84AGLVRR72 pKa = 11.84PSRR75 pKa = 11.84TVGMLRR81 pKa = 11.84LYY83 pKa = 10.69SEE85 pKa = 4.3EE86 pKa = 4.82DD87 pKa = 3.01IVRR90 pKa = 11.84LRR92 pKa = 11.84LIKK95 pKa = 10.6HH96 pKa = 5.86LVGDD100 pKa = 4.7LGLNIAGVEE109 pKa = 3.98LALGMFNQMLKK120 pKa = 10.02IKK122 pKa = 10.5SGLNQAEE129 pKa = 4.17KK130 pKa = 10.95SDD132 pKa = 3.59LKK134 pKa = 11.26SYY136 pKa = 11.4LEE138 pKa = 4.43DD139 pKa = 4.01CLNEE143 pKa = 4.02MFSVLRR149 pKa = 11.84VNPAA153 pKa = 2.72

Molecular weight: 17.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

855

0

855

250641

33

1494

293.1

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.39 ± 0.078

0.912 ± 0.027

5.95 ± 0.066

6.771 ± 0.071

3.95 ± 0.062

8.324 ± 0.073

2.058 ± 0.039

7.243 ± 0.062

4.492 ± 0.044

9.027 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.034

4.052 ± 0.046

4.468 ± 0.048

2.919 ± 0.039

5.13 ± 0.064

7.564 ± 0.079

5.479 ± 0.055

7.433 ± 0.072

1.313 ± 0.04

2.83 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski