Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Ignicoccus; Ignicoccus hospitalis

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ABP8|A8ABP8_IGNH4 Uncharacterized protein OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) OX=453591 GN=Igni_1173 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 3.92EE3 pKa = 4.79RR4 pKa = 11.84LVASLSGALCGAFAALGALSLSSGWEE30 pKa = 3.63AFVYY34 pKa = 10.28NAAAVPLLLGLLTAFLTYY52 pKa = 9.66MLSEE56 pKa = 4.35VLSS59 pKa = 3.88

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8A977|A8A977_IGNH4 Glyceraldehyde-3-phosphate ferredoxin oxidoreductase OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) OX=453591 GN=Igni_0296 PE=3 SV=1
MM1 pKa = 7.08VRR3 pKa = 11.84VAHH6 pKa = 5.87YY7 pKa = 10.31KK8 pKa = 10.25HH9 pKa = 5.61VAKK12 pKa = 10.62KK13 pKa = 9.97IRR15 pKa = 11.84LGKK18 pKa = 8.56KK19 pKa = 7.26TKK21 pKa = 8.67QNRR24 pKa = 11.84ALPAWVILKK33 pKa = 7.55TRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84VTRR40 pKa = 11.84NPMRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 8.61WRR48 pKa = 11.84RR49 pKa = 11.84QKK51 pKa = 10.4IKK53 pKa = 10.89NVV55 pKa = 3.17

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1434

0

1434

399079

33

1392

278.3

30.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.589 ± 0.084

1.032 ± 0.028

4.428 ± 0.056

8.699 ± 0.088

3.55 ± 0.04

7.367 ± 0.063

1.455 ± 0.027

4.921 ± 0.059

7.382 ± 0.068

10.992 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.033

2.608 ± 0.041

5.03 ± 0.047

1.535 ± 0.027

5.824 ± 0.057

5.438 ± 0.056

4.216 ± 0.085

9.783 ± 0.067

1.357 ± 0.028

3.605 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski