Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / KCTC 12902 / NCIMB 13706 / S10)
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4340 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7IME2|J7IME2_DESMD Cation diffusion facilitator family transporter OS=Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / KCTC 12902 / NCIMB 13706 / S10) OX=768704 GN=Desmer_0943 PE=4 SV=1
MM1 pKa = 7.59 FKK3 pKa = 10.03 TKK5 pKa = 10.44 QKK7 pKa = 10.63 ILGLVMSMALGLTFFVAPAPAAAATDD33 pKa = 3.96 MLTLANSDD41 pKa = 3.53 HH42 pKa = 5.85 FTILGEE48 pKa = 4.12 VDD50 pKa = 5.06 DD51 pKa = 4.94 EE52 pKa = 4.72 VSNIQVVGLDD62 pKa = 3.19 GTTWMTEE69 pKa = 3.94 EE70 pKa = 4.25 IADD73 pKa = 4.05 PEE75 pKa = 4.4 NIEE78 pKa = 4.04 WTTSDD83 pKa = 3.17 SSVVKK88 pKa = 10.38 FLDD91 pKa = 3.62 GTTEE95 pKa = 3.77 VATIDD100 pKa = 3.55 DD101 pKa = 4.1 TDD103 pKa = 3.79 TVKK106 pKa = 10.64 IKK108 pKa = 10.94 LLDD111 pKa = 3.49 EE112 pKa = 4.03 GRR114 pKa = 11.84 AYY116 pKa = 9.03 VTAHH120 pKa = 6.3 YY121 pKa = 11.03 DD122 pKa = 3.21 SMEE125 pKa = 3.5 ISAYY129 pKa = 10.46 VVVEE133 pKa = 4.1 TDD135 pKa = 3.62 GSATPSISGISVVVDD150 pKa = 3.76 APGTANDD157 pKa = 4.0 FTATNQTVYY166 pKa = 10.76 LADD169 pKa = 4.6 LSWLTDD175 pKa = 3.88 DD176 pKa = 5.86 SKK178 pKa = 10.14 TLQKK182 pKa = 10.86 NSSALHH188 pKa = 5.65 ALAMAGNTNYY198 pKa = 10.39 SDD200 pKa = 4.65 PDD202 pKa = 3.33 WAEE205 pKa = 3.7 NNLTVFSGGSYY216 pKa = 10.52 VYY218 pKa = 10.99 GIGSDD223 pKa = 3.87 FASGTDD229 pKa = 2.72 GWQYY233 pKa = 11.32 HH234 pKa = 4.94 VVHH237 pKa = 6.71 SNSTTDD243 pKa = 3.31 VPAYY247 pKa = 9.66 AASVYY252 pKa = 10.28 EE253 pKa = 4.33 LTSGDD258 pKa = 3.83 TVVWEE263 pKa = 4.13 YY264 pKa = 11.44 KK265 pKa = 10.29 GWW267 pKa = 3.56
Molecular weight: 28.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.973
Patrickios 1.901
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|J7ITW7|J7ITW7_DESMD ABC-type dipeptide/oligopeptide/nickel transport system ATPase component OS=Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / KCTC 12902 / NCIMB 13706 / S10) OX=768704 GN=Desmer_0519 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.19 QPKK8 pKa = 8.78 NRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 5.44 KK13 pKa = 10.09 RR14 pKa = 11.84 VHH16 pKa = 5.93 GFLSRR21 pKa = 11.84 MSTPTGRR28 pKa = 11.84 NVIKK32 pKa = 10.5 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.52 GRR39 pKa = 11.84 KK40 pKa = 8.8 KK41 pKa = 10.81 LSVV44 pKa = 3.15
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4340
0
4340
1328786
30
2859
306.2
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.562 ± 0.043
1.088 ± 0.016
4.895 ± 0.027
6.909 ± 0.045
4.096 ± 0.026
7.394 ± 0.056
1.754 ± 0.017
7.601 ± 0.032
6.219 ± 0.039
10.503 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.019
4.228 ± 0.03
3.858 ± 0.025
3.76 ± 0.028
4.534 ± 0.03
6.258 ± 0.03
5.3 ± 0.043
7.112 ± 0.034
1.05 ± 0.014
3.256 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here