Lactobacillus phage Sha1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9LUQ5|E9LUQ5_9CAUD Phage head-tail adaptor OS=Lactobacillus phage Sha1 OX=947981 PE=4 SV=1
MM1 pKa = 7.57AADD4 pKa = 4.68LKK6 pKa = 9.52TLKK9 pKa = 10.6SSLRR13 pKa = 11.84IDD15 pKa = 4.51GNDD18 pKa = 3.68DD19 pKa = 4.08DD20 pKa = 5.43EE21 pKa = 6.05LLTGYY26 pKa = 10.76LSAATSYY33 pKa = 10.39IKK35 pKa = 10.34QAIGDD40 pKa = 4.04EE41 pKa = 4.15NGVTGFYY48 pKa = 10.79EE49 pKa = 4.08MEE51 pKa = 4.23GVNDD55 pKa = 3.87LFEE58 pKa = 4.25TAVYY62 pKa = 10.69ALAGSYY68 pKa = 6.89WTYY71 pKa = 9.35RR72 pKa = 11.84TSITAIAVNPVDD84 pKa = 4.86LVVDD88 pKa = 4.49SIIGQLRR95 pKa = 11.84GLYY98 pKa = 8.73SQKK101 pKa = 10.41QYY103 pKa = 11.26EE104 pKa = 4.45AGTNDD109 pKa = 3.09EE110 pKa = 4.75SNN112 pKa = 3.23

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9LUT5|E9LUT5_9CAUD Prophage Lp3 protein 4-like protein OS=Lactobacillus phage Sha1 OX=947981 PE=4 SV=1
MM1 pKa = 8.08VEE3 pKa = 4.12VAVLTWALTSVWYY16 pKa = 9.66KK17 pKa = 10.39RR18 pKa = 11.84RR19 pKa = 11.84EE20 pKa = 3.56IRR22 pKa = 11.84NWFGII27 pKa = 3.84

Molecular weight: 3.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12440

27

1722

214.5

23.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.223 ± 0.661

0.442 ± 0.114

6.712 ± 0.364

4.879 ± 0.43

3.408 ± 0.261

6.849 ± 0.407

1.648 ± 0.163

6.6 ± 0.244

7.54 ± 0.707

7.645 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.181

6.262 ± 0.356

2.966 ± 0.293

4.791 ± 0.255

3.352 ± 0.301

7.371 ± 0.489

7.412 ± 0.587

6.125 ± 0.24

1.085 ± 0.093

4.076 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski