Amino acid dipepetide frequency for Lactobacillus phage Sha1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.824AlaAla: 4.824 ± 0.991
0.08AlaCys: 0.08 ± 0.069
4.582AlaAsp: 4.582 ± 0.546
4.341AlaGlu: 4.341 ± 0.756
2.331AlaPhe: 2.331 ± 0.365
6.029AlaGly: 6.029 ± 0.756
0.482AlaHis: 0.482 ± 0.166
6.512AlaIle: 6.512 ± 0.579
7.718AlaLys: 7.718 ± 1.328
6.512AlaLeu: 6.512 ± 0.672
2.733AlaMet: 2.733 ± 0.582
6.029AlaAsn: 6.029 ± 0.939
1.286AlaPro: 1.286 ± 0.316
3.698AlaGln: 3.698 ± 0.768
2.653AlaArg: 2.653 ± 0.486
6.673AlaSer: 6.673 ± 1.338
5.386AlaThr: 5.386 ± 0.806
5.065AlaVal: 5.065 ± 0.671
1.206AlaTrp: 1.206 ± 0.29
4.1AlaTyr: 4.1 ± 0.537
0.0AlaXaa: 0.0 ± 0.0
Cys
0.322CysAla: 0.322 ± 0.168
0.08CysCys: 0.08 ± 0.089
0.241CysAsp: 0.241 ± 0.191
0.241CysGlu: 0.241 ± 0.195
0.402CysPhe: 0.402 ± 0.192
0.643CysGly: 0.643 ± 0.265
0.161CysHis: 0.161 ± 0.107
0.0CysIle: 0.0 ± 0.0
0.161CysLys: 0.161 ± 0.114
0.402CysLeu: 0.402 ± 0.194
0.161CysMet: 0.161 ± 0.115
0.161CysAsn: 0.161 ± 0.105
0.402CysPro: 0.402 ± 0.204
0.322CysGln: 0.322 ± 0.167
0.161CysArg: 0.161 ± 0.178
0.161CysSer: 0.161 ± 0.11
0.161CysThr: 0.161 ± 0.1
0.161CysVal: 0.161 ± 0.097
0.0CysTrp: 0.0 ± 0.0
0.08CysTyr: 0.08 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
4.904AspAla: 4.904 ± 0.584
0.322AspCys: 0.322 ± 0.18
5.547AspAsp: 5.547 ± 0.955
4.18AspGlu: 4.18 ± 0.69
2.171AspPhe: 2.171 ± 0.512
5.386AspGly: 5.386 ± 0.744
0.965AspHis: 0.965 ± 0.37
3.296AspIle: 3.296 ± 0.506
5.386AspLys: 5.386 ± 0.635
4.502AspLeu: 4.502 ± 0.639
1.849AspMet: 1.849 ± 0.378
4.02AspAsn: 4.02 ± 0.492
2.733AspPro: 2.733 ± 0.737
3.216AspGln: 3.216 ± 0.541
2.331AspArg: 2.331 ± 0.465
4.743AspSer: 4.743 ± 0.57
4.18AspThr: 4.18 ± 0.639
3.939AspVal: 3.939 ± 0.571
0.965AspTrp: 0.965 ± 0.304
2.412AspTyr: 2.412 ± 0.599
0.0AspXaa: 0.0 ± 0.0
Glu
5.145GluAla: 5.145 ± 0.784
0.241GluCys: 0.241 ± 0.125
2.331GluAsp: 2.331 ± 0.47
2.573GluGlu: 2.573 ± 0.54
1.608GluPhe: 1.608 ± 0.317
2.09GluGly: 2.09 ± 0.454
1.045GluHis: 1.045 ± 0.297
3.216GluIle: 3.216 ± 0.483
4.1GluLys: 4.1 ± 0.682
5.386GluLeu: 5.386 ± 0.817
1.849GluMet: 1.849 ± 0.412
2.412GluAsn: 2.412 ± 0.47
1.206GluPro: 1.206 ± 0.408
3.055GluGln: 3.055 ± 0.554
1.849GluArg: 1.849 ± 0.387
2.894GluSer: 2.894 ± 0.482
3.216GluThr: 3.216 ± 0.446
2.251GluVal: 2.251 ± 0.457
0.322GluTrp: 0.322 ± 0.15
2.01GluTyr: 2.01 ± 0.377
0.0GluXaa: 0.0 ± 0.0
Phe
2.01PheAla: 2.01 ± 0.374
0.161PheCys: 0.161 ± 0.1
2.975PheAsp: 2.975 ± 0.76
1.849PheGlu: 1.849 ± 0.308
1.125PhePhe: 1.125 ± 0.263
2.975PheGly: 2.975 ± 0.463
0.724PheHis: 0.724 ± 0.269
2.331PheIle: 2.331 ± 0.527
2.251PheLys: 2.251 ± 0.457
2.331PheLeu: 2.331 ± 0.497
0.965PheMet: 0.965 ± 0.26
3.135PheAsn: 3.135 ± 0.58
0.884PhePro: 0.884 ± 0.24
0.965PheGln: 0.965 ± 0.241
0.563PheArg: 0.563 ± 0.171
2.733PheSer: 2.733 ± 0.398
2.653PheThr: 2.653 ± 0.551
1.608PheVal: 1.608 ± 0.376
0.241PheTrp: 0.241 ± 0.144
1.608PheTyr: 1.608 ± 0.384
0.0PheXaa: 0.0 ± 0.0
Gly
4.02GlyAla: 4.02 ± 0.881
0.08GlyCys: 0.08 ± 0.082
4.502GlyAsp: 4.502 ± 0.646
2.331GlyGlu: 2.331 ± 0.387
2.894GlyPhe: 2.894 ± 0.423
4.502GlyGly: 4.502 ± 0.695
1.527GlyHis: 1.527 ± 0.3
4.422GlyIle: 4.422 ± 0.427
5.547GlyLys: 5.547 ± 0.724
5.949GlyLeu: 5.949 ± 0.797
1.929GlyMet: 1.929 ± 0.428
5.306GlyAsn: 5.306 ± 0.734
0.965GlyPro: 0.965 ± 0.279
3.537GlyGln: 3.537 ± 0.563
2.09GlyArg: 2.09 ± 0.538
5.386GlySer: 5.386 ± 0.862
5.788GlyThr: 5.788 ± 0.708
3.939GlyVal: 3.939 ± 0.657
0.724GlyTrp: 0.724 ± 0.236
3.055GlyTyr: 3.055 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
1.286HisAla: 1.286 ± 0.359
0.08HisCys: 0.08 ± 0.079
0.965HisAsp: 0.965 ± 0.315
0.643HisGlu: 0.643 ± 0.19
0.804HisPhe: 0.804 ± 0.295
1.447HisGly: 1.447 ± 0.468
0.322HisHis: 0.322 ± 0.204
1.447HisIle: 1.447 ± 0.317
1.286HisLys: 1.286 ± 0.305
0.965HisLeu: 0.965 ± 0.295
0.241HisMet: 0.241 ± 0.132
0.643HisAsn: 0.643 ± 0.211
0.402HisPro: 0.402 ± 0.145
0.965HisGln: 0.965 ± 0.283
0.643HisArg: 0.643 ± 0.21
1.206HisSer: 1.206 ± 0.353
1.367HisThr: 1.367 ± 0.325
1.045HisVal: 1.045 ± 0.522
0.241HisTrp: 0.241 ± 0.12
0.482HisTyr: 0.482 ± 0.297
0.0HisXaa: 0.0 ± 0.0
Ile
5.386IleAla: 5.386 ± 0.612
0.482IleCys: 0.482 ± 0.207
5.226IleAsp: 5.226 ± 0.742
3.376IleGlu: 3.376 ± 0.554
1.688IlePhe: 1.688 ± 0.455
4.582IleGly: 4.582 ± 0.688
1.045IleHis: 1.045 ± 0.269
3.216IleIle: 3.216 ± 0.435
5.627IleLys: 5.627 ± 0.662
3.216IleLeu: 3.216 ± 0.6
2.01IleMet: 2.01 ± 0.415
4.663IleAsn: 4.663 ± 0.547
2.733IlePro: 2.733 ± 0.54
3.216IleGln: 3.216 ± 0.45
2.171IleArg: 2.171 ± 0.367
4.663IleSer: 4.663 ± 0.803
6.271IleThr: 6.271 ± 0.831
3.216IleVal: 3.216 ± 0.508
0.965IleTrp: 0.965 ± 0.218
2.251IleTyr: 2.251 ± 0.476
0.0IleXaa: 0.0 ± 0.0
Lys
8.2LysAla: 8.2 ± 1.527
0.08LysCys: 0.08 ± 0.079
4.341LysAsp: 4.341 ± 0.629
3.296LysGlu: 3.296 ± 0.61
2.814LysPhe: 2.814 ± 0.532
4.1LysGly: 4.1 ± 0.653
2.171LysHis: 2.171 ± 0.468
4.582LysIle: 4.582 ± 0.898
4.743LysLys: 4.743 ± 0.76
6.19LysLeu: 6.19 ± 0.698
2.01LysMet: 2.01 ± 0.39
2.975LysAsn: 2.975 ± 0.499
3.376LysPro: 3.376 ± 0.548
4.422LysGln: 4.422 ± 0.782
3.537LysArg: 3.537 ± 0.575
4.824LysSer: 4.824 ± 1.187
4.984LysThr: 4.984 ± 0.718
4.582LysVal: 4.582 ± 0.711
0.643LysTrp: 0.643 ± 0.277
3.537LysTyr: 3.537 ± 0.551
0.0LysXaa: 0.0 ± 0.0
Leu
6.833LeuAla: 6.833 ± 0.925
0.402LeuCys: 0.402 ± 0.221
5.386LeuAsp: 5.386 ± 0.69
3.618LeuGlu: 3.618 ± 0.559
2.171LeuPhe: 2.171 ± 0.429
4.984LeuGly: 4.984 ± 0.744
1.206LeuHis: 1.206 ± 0.31
5.306LeuIle: 5.306 ± 0.861
6.914LeuLys: 6.914 ± 0.775
4.984LeuLeu: 4.984 ± 0.77
2.01LeuMet: 2.01 ± 0.414
6.029LeuAsn: 6.029 ± 0.558
2.171LeuPro: 2.171 ± 0.426
3.055LeuGln: 3.055 ± 0.622
3.055LeuArg: 3.055 ± 0.464
5.547LeuSer: 5.547 ± 0.633
5.145LeuThr: 5.145 ± 0.718
4.261LeuVal: 4.261 ± 0.512
0.563LeuTrp: 0.563 ± 0.211
2.814LeuTyr: 2.814 ± 0.572
0.0LeuXaa: 0.0 ± 0.0
Met
3.618MetAla: 3.618 ± 0.414
0.241MetCys: 0.241 ± 0.126
1.688MetAsp: 1.688 ± 0.387
1.849MetGlu: 1.849 ± 0.361
0.884MetPhe: 0.884 ± 0.249
0.643MetGly: 0.643 ± 0.247
0.161MetHis: 0.161 ± 0.132
1.769MetIle: 1.769 ± 0.38
2.331MetLys: 2.331 ± 0.381
1.929MetLeu: 1.929 ± 0.353
0.161MetMet: 0.161 ± 0.092
1.688MetAsn: 1.688 ± 0.398
1.206MetPro: 1.206 ± 0.325
0.884MetGln: 0.884 ± 0.238
0.563MetArg: 0.563 ± 0.239
1.688MetSer: 1.688 ± 0.308
2.09MetThr: 2.09 ± 0.356
1.206MetVal: 1.206 ± 0.342
0.241MetTrp: 0.241 ± 0.137
1.286MetTyr: 1.286 ± 0.392
0.0MetXaa: 0.0 ± 0.0
Asn
5.145AsnAla: 5.145 ± 0.752
0.241AsnCys: 0.241 ± 0.143
4.18AsnAsp: 4.18 ± 0.558
2.733AsnGlu: 2.733 ± 0.515
1.929AsnPhe: 1.929 ± 0.32
7.155AsnGly: 7.155 ± 1.17
1.206AsnHis: 1.206 ± 0.308
3.698AsnIle: 3.698 ± 0.569
3.778AsnLys: 3.778 ± 0.613
4.341AsnLeu: 4.341 ± 0.545
1.929AsnMet: 1.929 ± 0.278
2.975AsnAsn: 2.975 ± 0.6
2.09AsnPro: 2.09 ± 0.393
2.573AsnGln: 2.573 ± 0.641
1.929AsnArg: 1.929 ± 0.457
4.743AsnSer: 4.743 ± 0.715
4.743AsnThr: 4.743 ± 0.802
4.341AsnVal: 4.341 ± 0.658
0.402AsnTrp: 0.402 ± 0.209
2.492AsnTyr: 2.492 ± 0.458
0.0AsnXaa: 0.0 ± 0.0
Pro
2.171ProAla: 2.171 ± 0.585
0.08ProCys: 0.08 ± 0.079
2.573ProAsp: 2.573 ± 0.553
2.251ProGlu: 2.251 ± 0.428
1.125ProPhe: 1.125 ± 0.361
1.206ProGly: 1.206 ± 0.294
0.161ProHis: 0.161 ± 0.121
2.01ProIle: 2.01 ± 0.544
2.492ProLys: 2.492 ± 0.586
2.01ProLeu: 2.01 ± 0.435
0.965ProMet: 0.965 ± 0.224
2.01ProAsn: 2.01 ± 0.356
0.643ProPro: 0.643 ± 0.225
1.125ProGln: 1.125 ± 0.272
1.045ProArg: 1.045 ± 0.24
2.09ProSer: 2.09 ± 0.419
2.09ProThr: 2.09 ± 0.334
2.09ProVal: 2.09 ± 0.427
0.241ProTrp: 0.241 ± 0.177
1.286ProTyr: 1.286 ± 0.44
0.0ProXaa: 0.0 ± 0.0
Gln
5.547GlnAla: 5.547 ± 0.797
0.322GlnCys: 0.322 ± 0.168
1.769GlnAsp: 1.769 ± 0.424
1.688GlnGlu: 1.688 ± 0.417
1.929GlnPhe: 1.929 ± 0.476
2.573GlnGly: 2.573 ± 0.565
0.563GlnHis: 0.563 ± 0.204
3.457GlnIle: 3.457 ± 0.6
2.492GlnLys: 2.492 ± 0.579
4.422GlnLeu: 4.422 ± 0.554
0.563GlnMet: 0.563 ± 0.185
2.01GlnAsn: 2.01 ± 0.407
1.367GlnPro: 1.367 ± 0.433
3.216GlnGln: 3.216 ± 0.558
1.286GlnArg: 1.286 ± 0.369
3.376GlnSer: 3.376 ± 0.557
3.778GlnThr: 3.778 ± 0.609
3.939GlnVal: 3.939 ± 0.824
0.322GlnTrp: 0.322 ± 0.161
3.296GlnTyr: 3.296 ± 0.511
0.0GlnXaa: 0.0 ± 0.0
Arg
2.171ArgAla: 2.171 ± 0.473
0.08ArgCys: 0.08 ± 0.086
2.492ArgAsp: 2.492 ± 0.619
2.975ArgGlu: 2.975 ± 0.604
1.045ArgPhe: 1.045 ± 0.289
1.527ArgGly: 1.527 ± 0.337
0.482ArgHis: 0.482 ± 0.241
1.929ArgIle: 1.929 ± 0.37
1.769ArgLys: 1.769 ± 0.581
2.251ArgLeu: 2.251 ± 0.391
0.884ArgMet: 0.884 ± 0.278
2.09ArgAsn: 2.09 ± 0.332
0.724ArgPro: 0.724 ± 0.211
1.608ArgGln: 1.608 ± 0.541
1.447ArgArg: 1.447 ± 0.354
1.849ArgSer: 1.849 ± 0.324
2.894ArgThr: 2.894 ± 0.389
2.975ArgVal: 2.975 ± 0.469
0.804ArgTrp: 0.804 ± 0.268
1.527ArgTyr: 1.527 ± 0.472
0.0ArgXaa: 0.0 ± 0.0
Ser
6.11SerAla: 6.11 ± 1.062
0.402SerCys: 0.402 ± 0.196
5.145SerAsp: 5.145 ± 0.521
2.733SerGlu: 2.733 ± 0.661
3.296SerPhe: 3.296 ± 0.64
6.351SerGly: 6.351 ± 0.635
1.608SerHis: 1.608 ± 0.38
5.065SerIle: 5.065 ± 0.751
4.663SerLys: 4.663 ± 0.916
5.386SerLeu: 5.386 ± 0.709
2.01SerMet: 2.01 ± 0.389
4.502SerAsn: 4.502 ± 0.794
1.527SerPro: 1.527 ± 0.308
5.306SerGln: 5.306 ± 0.847
2.573SerArg: 2.573 ± 0.328
5.708SerSer: 5.708 ± 1.018
3.698SerThr: 3.698 ± 0.666
5.467SerVal: 5.467 ± 0.621
0.804SerTrp: 0.804 ± 0.284
1.367SerTyr: 1.367 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
6.592ThrAla: 6.592 ± 0.863
0.241ThrCys: 0.241 ± 0.137
4.663ThrAsp: 4.663 ± 0.74
2.814ThrGlu: 2.814 ± 0.399
2.573ThrPhe: 2.573 ± 0.44
5.949ThrGly: 5.949 ± 0.797
0.804ThrHis: 0.804 ± 0.249
6.271ThrIle: 6.271 ± 0.678
5.306ThrLys: 5.306 ± 0.731
5.788ThrLeu: 5.788 ± 0.749
1.367ThrMet: 1.367 ± 0.296
4.341ThrAsn: 4.341 ± 0.732
2.331ThrPro: 2.331 ± 0.472
2.412ThrGln: 2.412 ± 0.528
2.573ThrArg: 2.573 ± 0.428
5.869ThrSer: 5.869 ± 0.833
6.512ThrThr: 6.512 ± 1.268
4.02ThrVal: 4.02 ± 0.701
1.045ThrTrp: 1.045 ± 0.281
2.653ThrTyr: 2.653 ± 0.751
0.0ThrXaa: 0.0 ± 0.0
Val
4.502ValAla: 4.502 ± 0.597
0.402ValCys: 0.402 ± 0.172
5.306ValAsp: 5.306 ± 0.765
2.653ValGlu: 2.653 ± 0.53
1.769ValPhe: 1.769 ± 0.425
3.778ValGly: 3.778 ± 0.707
0.884ValHis: 0.884 ± 0.311
4.502ValIle: 4.502 ± 0.493
5.145ValLys: 5.145 ± 0.71
4.582ValLeu: 4.582 ± 0.578
1.286ValMet: 1.286 ± 0.373
4.18ValAsn: 4.18 ± 0.449
2.01ValPro: 2.01 ± 0.284
1.447ValGln: 1.447 ± 0.29
1.367ValArg: 1.367 ± 0.42
5.708ValSer: 5.708 ± 0.64
4.904ValThr: 4.904 ± 0.734
4.261ValVal: 4.261 ± 0.468
0.643ValTrp: 0.643 ± 0.2
1.929ValTyr: 1.929 ± 0.42
0.0ValXaa: 0.0 ± 0.0
Trp
0.724TrpAla: 0.724 ± 0.206
0.08TrpCys: 0.08 ± 0.085
0.724TrpAsp: 0.724 ± 0.244
0.643TrpGlu: 0.643 ± 0.222
0.322TrpPhe: 0.322 ± 0.194
0.322TrpGly: 0.322 ± 0.154
0.241TrpHis: 0.241 ± 0.147
0.724TrpIle: 0.724 ± 0.322
0.563TrpLys: 0.563 ± 0.199
1.608TrpLeu: 1.608 ± 0.306
0.402TrpMet: 0.402 ± 0.173
0.563TrpAsn: 0.563 ± 0.235
0.161TrpPro: 0.161 ± 0.108
0.643TrpGln: 0.643 ± 0.194
0.402TrpArg: 0.402 ± 0.191
0.965TrpSer: 0.965 ± 0.275
0.724TrpThr: 0.724 ± 0.26
0.402TrpVal: 0.402 ± 0.158
0.161TrpTrp: 0.161 ± 0.092
0.482TrpTyr: 0.482 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.733TyrAla: 2.733 ± 0.332
0.322TyrCys: 0.322 ± 0.206
2.492TyrAsp: 2.492 ± 0.345
2.01TyrGlu: 2.01 ± 0.332
1.206TyrPhe: 1.206 ± 0.285
2.251TyrGly: 2.251 ± 0.586
0.724TyrHis: 0.724 ± 0.198
2.251TyrIle: 2.251 ± 0.582
3.135TyrLys: 3.135 ± 0.514
3.698TyrLeu: 3.698 ± 0.75
0.724TyrMet: 0.724 ± 0.194
2.894TyrAsn: 2.894 ± 0.707
1.447TyrPro: 1.447 ± 0.427
2.251TyrGln: 2.251 ± 0.4
1.286TyrArg: 1.286 ± 0.331
2.894TyrSer: 2.894 ± 0.358
3.537TyrThr: 3.537 ± 0.786
2.492TyrVal: 2.492 ± 0.449
0.322TyrTrp: 0.322 ± 0.161
2.09TyrTyr: 2.09 ± 0.468
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12440 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski