Klebsiella phage Magnus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Taipeivirus; Klebsiella virus Magnus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N795|A0A5B9N795_9CAUD Uncharacterized protein OS=Klebsiella phage Magnus OX=2589660 GN=CPT_Magnus_041 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.09HH3 pKa = 6.12NKK5 pKa = 9.06ILLVCALAFSTTACSTLLDD24 pKa = 4.01VPDD27 pKa = 4.4LSGPDD32 pKa = 3.58FTQDD36 pKa = 3.04QAQTKK41 pKa = 8.7MDD43 pKa = 4.05DD44 pKa = 3.46MVKK47 pKa = 10.47AHH49 pKa = 6.74ATLQGTTPGPIQTVCNYY66 pKa = 10.46DD67 pKa = 3.73DD68 pKa = 4.68SDD70 pKa = 3.91PTTEE74 pKa = 5.24LYY76 pKa = 10.56HH77 pKa = 6.97CSTFVKK83 pKa = 10.55DD84 pKa = 4.05SMVVLYY90 pKa = 10.14GDD92 pKa = 4.43CQADD96 pKa = 3.94GCKK99 pKa = 9.49ATGYY103 pKa = 11.13DD104 pKa = 4.02KK105 pKa = 11.61VEE107 pKa = 3.94EE108 pKa = 4.53DD109 pKa = 3.61KK110 pKa = 11.58

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N1C6|A0A5B9N1C6_9CAUD Uncharacterized protein OS=Klebsiella phage Magnus OX=2589660 GN=CPT_Magnus_048 PE=4 SV=1
MM1 pKa = 7.4FLLSSRR7 pKa = 11.84ILGAPTGRR15 pKa = 11.84GRR17 pKa = 11.84MRR19 pKa = 11.84KK20 pKa = 8.8FRR22 pKa = 11.84QIKK25 pKa = 10.06SFVNKK30 pKa = 10.12GG31 pKa = 3.07

Molecular weight: 3.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

48702

30

1612

229.7

25.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.755 ± 0.176

1.187 ± 0.058

6.507 ± 0.112

6.451 ± 0.2

4.312 ± 0.106

6.632 ± 0.152

1.827 ± 0.096

6.308 ± 0.14

6.39 ± 0.156

8.039 ± 0.138

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.858 ± 0.096

5.203 ± 0.125

3.873 ± 0.108

3.778 ± 0.095

4.807 ± 0.141

6.571 ± 0.167

5.938 ± 0.189

7.102 ± 0.163

1.462 ± 0.07

4.0 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski