Amino acid dipepetide frequency for Klebsiella phage Magnus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.585AlaAla: 5.585 ± 0.465
0.595AlaCys: 0.595 ± 0.112
4.497AlaAsp: 4.497 ± 0.294
4.538AlaGlu: 4.538 ± 0.394
2.731AlaPhe: 2.731 ± 0.231
4.825AlaGly: 4.825 ± 0.373
1.15AlaHis: 1.15 ± 0.135
4.23AlaIle: 4.23 ± 0.345
4.025AlaLys: 4.025 ± 0.296
5.339AlaLeu: 5.339 ± 0.318
1.869AlaMet: 1.869 ± 0.194
3.059AlaAsn: 3.059 ± 0.296
2.526AlaPro: 2.526 ± 0.263
2.628AlaGln: 2.628 ± 0.297
3.388AlaArg: 3.388 ± 0.27
4.127AlaSer: 4.127 ± 0.352
4.066AlaThr: 4.066 ± 0.326
4.825AlaVal: 4.825 ± 0.334
0.924AlaTrp: 0.924 ± 0.129
2.628AlaTyr: 2.628 ± 0.25
0.0AlaXaa: 0.0 ± 0.0
Cys
0.637CysAla: 0.637 ± 0.122
0.185CysCys: 0.185 ± 0.061
0.78CysAsp: 0.78 ± 0.137
1.006CysGlu: 1.006 ± 0.148
0.308CysPhe: 0.308 ± 0.075
0.842CysGly: 0.842 ± 0.134
0.308CysHis: 0.308 ± 0.09
0.821CysIle: 0.821 ± 0.122
0.637CysLys: 0.637 ± 0.121
0.739CysLeu: 0.739 ± 0.126
0.349CysMet: 0.349 ± 0.077
0.739CysAsn: 0.739 ± 0.119
0.493CysPro: 0.493 ± 0.1
0.308CysGln: 0.308 ± 0.08
0.575CysArg: 0.575 ± 0.103
0.924CysSer: 0.924 ± 0.143
0.575CysThr: 0.575 ± 0.108
1.006CysVal: 1.006 ± 0.147
0.267CysTrp: 0.267 ± 0.07
0.37CysTyr: 0.37 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
5.01AspAla: 5.01 ± 0.365
0.678AspCys: 0.678 ± 0.105
4.353AspAsp: 4.353 ± 0.409
3.901AspGlu: 3.901 ± 0.301
3.265AspPhe: 3.265 ± 0.227
4.764AspGly: 4.764 ± 0.321
1.15AspHis: 1.15 ± 0.173
4.23AspIle: 4.23 ± 0.286
3.737AspLys: 3.737 ± 0.268
5.215AspLeu: 5.215 ± 0.407
2.053AspMet: 2.053 ± 0.179
3.162AspAsn: 3.162 ± 0.271
3.183AspPro: 3.183 ± 0.258
2.033AspGln: 2.033 ± 0.211
2.156AspArg: 2.156 ± 0.237
3.922AspSer: 3.922 ± 0.309
3.367AspThr: 3.367 ± 0.253
4.312AspVal: 4.312 ± 0.259
1.068AspTrp: 1.068 ± 0.164
3.511AspTyr: 3.511 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
4.127GluAla: 4.127 ± 0.408
0.76GluCys: 0.76 ± 0.13
4.148GluAsp: 4.148 ± 0.336
4.353GluGlu: 4.353 ± 0.384
2.875GluPhe: 2.875 ± 0.242
4.045GluGly: 4.045 ± 0.353
1.458GluHis: 1.458 ± 0.141
4.661GluIle: 4.661 ± 0.281
4.004GluLys: 4.004 ± 0.388
5.955GluLeu: 5.955 ± 0.323
2.156GluMet: 2.156 ± 0.257
2.71GluAsn: 2.71 ± 0.214
1.93GluPro: 1.93 ± 0.212
2.669GluGln: 2.669 ± 0.21
3.409GluArg: 3.409 ± 0.308
3.347GluSer: 3.347 ± 0.297
3.573GluThr: 3.573 ± 0.276
4.312GluVal: 4.312 ± 0.318
0.986GluTrp: 0.986 ± 0.147
3.039GluTyr: 3.039 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
2.526PheAla: 2.526 ± 0.259
0.616PheCys: 0.616 ± 0.13
3.039PheAsp: 3.039 ± 0.287
2.895PheGlu: 2.895 ± 0.293
1.745PhePhe: 1.745 ± 0.205
2.998PheGly: 2.998 ± 0.259
0.698PheHis: 0.698 ± 0.112
2.587PheIle: 2.587 ± 0.214
2.608PheLys: 2.608 ± 0.214
3.121PheLeu: 3.121 ± 0.275
1.335PheMet: 1.335 ± 0.19
2.567PheAsn: 2.567 ± 0.216
1.478PhePro: 1.478 ± 0.204
1.602PheGln: 1.602 ± 0.164
2.177PheArg: 2.177 ± 0.239
3.265PheSer: 3.265 ± 0.252
2.751PheThr: 2.751 ± 0.218
2.793PheVal: 2.793 ± 0.211
0.739PheTrp: 0.739 ± 0.123
1.581PheTyr: 1.581 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
3.573GlyAla: 3.573 ± 0.315
1.006GlyCys: 1.006 ± 0.145
3.655GlyAsp: 3.655 ± 0.288
4.394GlyGlu: 4.394 ± 0.315
2.834GlyPhe: 2.834 ± 0.283
4.825GlyGly: 4.825 ± 0.434
1.253GlyHis: 1.253 ± 0.174
4.579GlyIle: 4.579 ± 0.323
5.482GlyLys: 5.482 ± 0.301
5.051GlyLeu: 5.051 ± 0.317
2.053GlyMet: 2.053 ± 0.203
3.285GlyAsn: 3.285 ± 0.23
1.273GlyPro: 1.273 ± 0.189
2.649GlyGln: 2.649 ± 0.218
3.244GlyArg: 3.244 ± 0.268
4.558GlySer: 4.558 ± 0.418
3.963GlyThr: 3.963 ± 0.307
4.907GlyVal: 4.907 ± 0.336
1.294GlyTrp: 1.294 ± 0.166
2.443GlyTyr: 2.443 ± 0.248
0.0GlyXaa: 0.0 ± 0.0
His
0.965HisAla: 0.965 ± 0.147
0.287HisCys: 0.287 ± 0.073
1.232HisAsp: 1.232 ± 0.143
0.76HisGlu: 0.76 ± 0.117
0.986HisPhe: 0.986 ± 0.138
1.088HisGly: 1.088 ± 0.167
0.431HisHis: 0.431 ± 0.094
1.396HisIle: 1.396 ± 0.191
1.17HisLys: 1.17 ± 0.174
1.786HisLeu: 1.786 ± 0.174
0.595HisMet: 0.595 ± 0.104
0.883HisAsn: 0.883 ± 0.137
0.924HisPro: 0.924 ± 0.156
0.719HisGln: 0.719 ± 0.104
0.801HisArg: 0.801 ± 0.136
1.027HisSer: 1.027 ± 0.134
1.088HisThr: 1.088 ± 0.132
1.129HisVal: 1.129 ± 0.167
0.246HisTrp: 0.246 ± 0.076
0.76HisTyr: 0.76 ± 0.12
0.0HisXaa: 0.0 ± 0.0
Ile
4.291IleAla: 4.291 ± 0.323
0.862IleCys: 0.862 ± 0.148
4.805IleAsp: 4.805 ± 0.307
4.866IleGlu: 4.866 ± 0.303
1.663IlePhe: 1.663 ± 0.201
3.881IleGly: 3.881 ± 0.297
1.129IleHis: 1.129 ± 0.14
3.901IleIle: 3.901 ± 0.286
4.086IleLys: 4.086 ± 0.303
4.641IleLeu: 4.641 ± 0.327
1.376IleMet: 1.376 ± 0.161
3.491IleAsn: 3.491 ± 0.279
3.183IlePro: 3.183 ± 0.234
2.669IleGln: 2.669 ± 0.238
2.916IleArg: 2.916 ± 0.241
4.209IleSer: 4.209 ± 0.39
4.086IleThr: 4.086 ± 0.324
3.922IleVal: 3.922 ± 0.247
0.842IleTrp: 0.842 ± 0.14
2.259IleTyr: 2.259 ± 0.205
0.0IleXaa: 0.0 ± 0.0
Lys
4.312LysAla: 4.312 ± 0.39
0.534LysCys: 0.534 ± 0.118
4.004LysAsp: 4.004 ± 0.257
4.312LysGlu: 4.312 ± 0.381
3.203LysPhe: 3.203 ± 0.223
4.107LysGly: 4.107 ± 0.296
1.088LysHis: 1.088 ± 0.203
3.922LysIle: 3.922 ± 0.292
4.086LysLys: 4.086 ± 0.4
4.723LysLeu: 4.723 ± 0.328
2.567LysMet: 2.567 ± 0.284
2.834LysAsn: 2.834 ± 0.269
2.361LysPro: 2.361 ± 0.224
2.464LysGln: 2.464 ± 0.256
2.916LysArg: 2.916 ± 0.259
4.374LysSer: 4.374 ± 0.313
4.148LysThr: 4.148 ± 0.249
4.476LysVal: 4.476 ± 0.275
1.047LysTrp: 1.047 ± 0.154
2.402LysTyr: 2.402 ± 0.199
0.0LysXaa: 0.0 ± 0.0
Leu
5.708LeuAla: 5.708 ± 0.395
0.678LeuCys: 0.678 ± 0.125
5.113LeuAsp: 5.113 ± 0.343
5.503LeuGlu: 5.503 ± 0.304
3.634LeuPhe: 3.634 ± 0.259
5.031LeuGly: 5.031 ± 0.335
1.396LeuHis: 1.396 ± 0.158
4.107LeuIle: 4.107 ± 0.295
6.365LeuLys: 6.365 ± 0.424
6.078LeuLeu: 6.078 ± 0.364
1.992LeuMet: 1.992 ± 0.202
4.435LeuAsn: 4.435 ± 0.339
3.491LeuPro: 3.491 ± 0.277
2.71LeuGln: 2.71 ± 0.237
3.758LeuArg: 3.758 ± 0.256
6.037LeuSer: 6.037 ± 0.379
4.517LeuThr: 4.517 ± 0.347
5.975LeuVal: 5.975 ± 0.346
0.842LeuTrp: 0.842 ± 0.128
3.018LeuTyr: 3.018 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
2.382MetAla: 2.382 ± 0.222
0.287MetCys: 0.287 ± 0.084
1.684MetAsp: 1.684 ± 0.209
1.499MetGlu: 1.499 ± 0.179
1.396MetPhe: 1.396 ± 0.173
1.622MetGly: 1.622 ± 0.191
0.411MetHis: 0.411 ± 0.097
1.766MetIle: 1.766 ± 0.169
2.361MetLys: 2.361 ± 0.279
2.259MetLeu: 2.259 ± 0.201
0.821MetMet: 0.821 ± 0.118
1.745MetAsn: 1.745 ± 0.221
0.986MetPro: 0.986 ± 0.13
0.903MetGln: 0.903 ± 0.152
1.663MetArg: 1.663 ± 0.189
2.279MetSer: 2.279 ± 0.235
1.519MetThr: 1.519 ± 0.187
1.807MetVal: 1.807 ± 0.189
0.287MetTrp: 0.287 ± 0.08
0.903MetTyr: 0.903 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.922AsnAla: 3.922 ± 0.317
0.575AsnCys: 0.575 ± 0.118
2.916AsnAsp: 2.916 ± 0.223
2.464AsnGlu: 2.464 ± 0.243
2.238AsnPhe: 2.238 ± 0.205
4.025AsnGly: 4.025 ± 0.338
0.986AsnHis: 0.986 ± 0.142
3.511AsnIle: 3.511 ± 0.339
3.059AsnLys: 3.059 ± 0.253
3.799AsnLeu: 3.799 ± 0.286
1.602AsnMet: 1.602 ± 0.21
3.634AsnAsn: 3.634 ± 0.281
2.464AsnPro: 2.464 ± 0.229
1.807AsnGln: 1.807 ± 0.204
2.546AsnArg: 2.546 ± 0.296
3.306AsnSer: 3.306 ± 0.287
3.08AsnThr: 3.08 ± 0.239
3.737AsnVal: 3.737 ± 0.309
0.657AsnTrp: 0.657 ± 0.123
1.704AsnTyr: 1.704 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
2.69ProAla: 2.69 ± 0.242
0.554ProCys: 0.554 ± 0.11
2.916ProAsp: 2.916 ± 0.242
3.224ProGlu: 3.224 ± 0.281
1.786ProPhe: 1.786 ± 0.199
2.402ProGly: 2.402 ± 0.213
0.657ProHis: 0.657 ± 0.111
1.951ProIle: 1.951 ± 0.213
2.485ProLys: 2.485 ± 0.267
3.224ProLeu: 3.224 ± 0.261
0.76ProMet: 0.76 ± 0.132
1.622ProAsn: 1.622 ± 0.189
1.129ProPro: 1.129 ± 0.194
1.396ProGln: 1.396 ± 0.173
1.786ProArg: 1.786 ± 0.193
2.361ProSer: 2.361 ± 0.232
2.628ProThr: 2.628 ± 0.224
3.039ProVal: 3.039 ± 0.255
0.493ProTrp: 0.493 ± 0.11
1.622ProTyr: 1.622 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
2.526GlnAla: 2.526 ± 0.263
0.452GlnCys: 0.452 ± 0.108
2.3GlnAsp: 2.3 ± 0.238
2.464GlnGlu: 2.464 ± 0.297
2.135GlnPhe: 2.135 ± 0.198
2.259GlnGly: 2.259 ± 0.205
0.719GlnHis: 0.719 ± 0.137
2.772GlnIle: 2.772 ± 0.254
2.279GlnLys: 2.279 ± 0.243
3.162GlnLeu: 3.162 ± 0.277
1.17GlnMet: 1.17 ± 0.168
1.684GlnAsn: 1.684 ± 0.15
1.232GlnPro: 1.232 ± 0.181
1.54GlnGln: 1.54 ± 0.212
1.766GlnArg: 1.766 ± 0.2
2.341GlnSer: 2.341 ± 0.236
2.464GlnThr: 2.464 ± 0.215
2.382GlnVal: 2.382 ± 0.23
0.554GlnTrp: 0.554 ± 0.103
1.581GlnTyr: 1.581 ± 0.173
0.0GlnXaa: 0.0 ± 0.0
Arg
2.875ArgAla: 2.875 ± 0.249
0.821ArgCys: 0.821 ± 0.146
2.69ArgAsp: 2.69 ± 0.263
3.285ArgGlu: 3.285 ± 0.286
1.992ArgPhe: 1.992 ± 0.224
3.08ArgGly: 3.08 ± 0.299
0.945ArgHis: 0.945 ± 0.139
3.203ArgIle: 3.203 ± 0.294
2.772ArgLys: 2.772 ± 0.275
4.784ArgLeu: 4.784 ± 0.358
1.335ArgMet: 1.335 ± 0.194
2.505ArgAsn: 2.505 ± 0.255
1.581ArgPro: 1.581 ± 0.187
2.012ArgGln: 2.012 ± 0.215
2.772ArgArg: 2.772 ± 0.27
3.018ArgSer: 3.018 ± 0.242
2.32ArgThr: 2.32 ± 0.213
3.121ArgVal: 3.121 ± 0.259
0.739ArgTrp: 0.739 ± 0.135
2.218ArgTyr: 2.218 ± 0.225
0.0ArgXaa: 0.0 ± 0.0
Ser
3.552SerAla: 3.552 ± 0.32
0.719SerCys: 0.719 ± 0.132
3.942SerAsp: 3.942 ± 0.232
3.86SerGlu: 3.86 ± 0.321
2.731SerPhe: 2.731 ± 0.227
4.969SerGly: 4.969 ± 0.369
1.232SerHis: 1.232 ± 0.139
4.497SerIle: 4.497 ± 0.345
3.655SerLys: 3.655 ± 0.274
5.893SerLeu: 5.893 ± 0.368
1.786SerMet: 1.786 ± 0.243
3.737SerAsn: 3.737 ± 0.295
2.526SerPro: 2.526 ± 0.251
3.039SerGln: 3.039 ± 0.273
3.018SerArg: 3.018 ± 0.246
4.682SerSer: 4.682 ± 0.407
4.004SerThr: 4.004 ± 0.398
4.333SerVal: 4.333 ± 0.342
0.719SerTrp: 0.719 ± 0.111
2.813SerTyr: 2.813 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
4.168ThrAla: 4.168 ± 0.327
0.637ThrCys: 0.637 ± 0.116
3.552ThrAsp: 3.552 ± 0.252
3.162ThrGlu: 3.162 ± 0.267
2.402ThrPhe: 2.402 ± 0.171
4.086ThrGly: 4.086 ± 0.32
0.801ThrHis: 0.801 ± 0.131
3.963ThrIle: 3.963 ± 0.276
3.429ThrLys: 3.429 ± 0.258
4.866ThrLeu: 4.866 ± 0.293
1.499ThrMet: 1.499 ± 0.201
2.977ThrAsn: 2.977 ± 0.234
3.265ThrPro: 3.265 ± 0.247
2.094ThrGln: 2.094 ± 0.218
2.751ThrArg: 2.751 ± 0.23
4.127ThrSer: 4.127 ± 0.342
3.819ThrThr: 3.819 ± 0.41
4.907ThrVal: 4.907 ± 0.469
0.903ThrTrp: 0.903 ± 0.119
1.971ThrTyr: 1.971 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
4.866ValAla: 4.866 ± 0.299
0.698ValCys: 0.698 ± 0.137
5.565ValAsp: 5.565 ± 0.31
4.661ValGlu: 4.661 ± 0.289
2.875ValPhe: 2.875 ± 0.244
4.086ValGly: 4.086 ± 0.286
1.232ValHis: 1.232 ± 0.174
4.086ValIle: 4.086 ± 0.321
4.538ValLys: 4.538 ± 0.27
5.298ValLeu: 5.298 ± 0.284
1.663ValMet: 1.663 ± 0.18
3.696ValAsn: 3.696 ± 0.274
2.854ValPro: 2.854 ± 0.25
2.649ValGln: 2.649 ± 0.275
3.409ValArg: 3.409 ± 0.293
4.415ValSer: 4.415 ± 0.329
4.333ValThr: 4.333 ± 0.433
5.79ValVal: 5.79 ± 0.416
1.211ValTrp: 1.211 ± 0.159
3.101ValTyr: 3.101 ± 0.278
0.0ValXaa: 0.0 ± 0.0
Trp
1.253TrpAla: 1.253 ± 0.181
0.287TrpCys: 0.287 ± 0.098
1.027TrpAsp: 1.027 ± 0.158
1.273TrpGlu: 1.273 ± 0.176
0.698TrpPhe: 0.698 ± 0.133
0.739TrpGly: 0.739 ± 0.13
0.164TrpHis: 0.164 ± 0.049
0.616TrpIle: 0.616 ± 0.113
0.821TrpLys: 0.821 ± 0.139
1.355TrpLeu: 1.355 ± 0.169
0.472TrpMet: 0.472 ± 0.097
0.842TrpAsn: 0.842 ± 0.108
0.329TrpPro: 0.329 ± 0.08
0.37TrpGln: 0.37 ± 0.089
1.027TrpArg: 1.027 ± 0.139
0.678TrpSer: 0.678 ± 0.109
0.678TrpThr: 0.678 ± 0.13
1.294TrpVal: 1.294 ± 0.177
0.164TrpTrp: 0.164 ± 0.056
0.534TrpTyr: 0.534 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.587TyrAla: 2.587 ± 0.213
0.637TyrCys: 0.637 ± 0.125
2.854TyrAsp: 2.854 ± 0.242
2.094TyrGlu: 2.094 ± 0.226
1.622TyrPhe: 1.622 ± 0.167
2.649TyrGly: 2.649 ± 0.247
1.068TyrHis: 1.068 ± 0.156
2.279TyrIle: 2.279 ± 0.218
2.3TyrLys: 2.3 ± 0.198
3.101TyrLeu: 3.101 ± 0.262
1.109TyrMet: 1.109 ± 0.165
2.423TyrAsn: 2.423 ± 0.254
1.519TyrPro: 1.519 ± 0.165
1.519TyrGln: 1.519 ± 0.208
1.992TyrArg: 1.992 ± 0.223
2.71TyrSer: 2.71 ± 0.215
2.402TyrThr: 2.402 ± 0.311
2.957TyrVal: 2.957 ± 0.281
0.637TyrTrp: 0.637 ± 0.109
1.54TyrTyr: 1.54 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 212 proteins (48702 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski