Enterococcus phage phiFL3A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Phifelvirus; Enterococcus virus FL3

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2IZS4|D2IZS4_9CAUD Uncharacterized protein gp32 OS=Enterococcus phage phiFL3A OX=673837 GN=gp32 PE=4 SV=1
MM1 pKa = 7.72GEE3 pKa = 3.9IAEE6 pKa = 4.25MMLEE10 pKa = 4.23GVLCASCGVFLDD22 pKa = 4.35VYY24 pKa = 10.63GNGYY28 pKa = 9.37PEE30 pKa = 4.12YY31 pKa = 10.98CEE33 pKa = 5.4DD34 pKa = 3.74CQEE37 pKa = 4.13QIIEE41 pKa = 4.03EE42 pKa = 4.18DD43 pKa = 3.31HH44 pKa = 6.38RR45 pKa = 5.97

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2IZT2|D2IZT2_9CAUD Terminase small subunit OS=Enterococcus phage phiFL3A OX=673837 GN=gp40 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.69FLRR5 pKa = 11.84WIFVDD10 pKa = 3.79NDD12 pKa = 3.81GNFQWMSITALISAGGVWATFNRR35 pKa = 11.84SKK37 pKa = 10.58KK38 pKa = 9.01QYY40 pKa = 10.33RR41 pKa = 11.84LTFILKK47 pKa = 9.43QRR49 pKa = 11.84LKK51 pKa = 10.88SS52 pKa = 3.5

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

11777

32

1511

184.0

20.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.581 ± 0.404

0.594 ± 0.12

6.063 ± 0.297

8.05 ± 0.519

3.787 ± 0.166

6.326 ± 0.489

1.274 ± 0.136

6.997 ± 0.232

9.442 ± 0.357

8.695 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.134

5.859 ± 0.247

3.006 ± 0.183

3.872 ± 0.249

3.965 ± 0.275

6.53 ± 0.382

5.231 ± 0.258

6.249 ± 0.203

1.265 ± 0.139

3.855 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski