Amino acid dipepetide frequency for Enterococcus phage phiFL3A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.567AlaAla: 3.567 ± 0.965
0.34AlaCys: 0.34 ± 0.171
2.463AlaAsp: 2.463 ± 0.464
5.435AlaGlu: 5.435 ± 0.711
2.972AlaPhe: 2.972 ± 0.759
3.651AlaGly: 3.651 ± 0.469
0.679AlaHis: 0.679 ± 0.287
6.284AlaIle: 6.284 ± 0.646
5.774AlaLys: 5.774 ± 1.085
7.303AlaLeu: 7.303 ± 0.807
1.783AlaMet: 1.783 ± 0.353
3.397AlaAsn: 3.397 ± 0.498
1.189AlaPro: 1.189 ± 0.341
1.444AlaGln: 1.444 ± 0.361
2.208AlaArg: 2.208 ± 0.476
3.227AlaSer: 3.227 ± 0.832
4.586AlaThr: 4.586 ± 0.596
5.01AlaVal: 5.01 ± 0.715
1.529AlaTrp: 1.529 ± 0.69
2.972AlaTyr: 2.972 ± 0.603
0.0AlaXaa: 0.0 ± 0.0
Cys
0.34CysAla: 0.34 ± 0.242
0.0CysCys: 0.0 ± 0.0
0.17CysAsp: 0.17 ± 0.135
0.51CysGlu: 0.51 ± 0.202
0.34CysPhe: 0.34 ± 0.201
0.594CysGly: 0.594 ± 0.27
0.0CysHis: 0.0 ± 0.0
0.51CysIle: 0.51 ± 0.226
0.764CysLys: 0.764 ± 0.278
0.594CysLeu: 0.594 ± 0.261
0.0CysMet: 0.0 ± 0.0
0.255CysAsn: 0.255 ± 0.138
0.425CysPro: 0.425 ± 0.195
0.425CysGln: 0.425 ± 0.219
0.17CysArg: 0.17 ± 0.133
0.255CysSer: 0.255 ± 0.177
0.17CysThr: 0.17 ± 0.108
0.17CysVal: 0.17 ± 0.117
0.085CysTrp: 0.085 ± 0.091
0.17CysTyr: 0.17 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
4.331AspAla: 4.331 ± 0.756
0.34AspCys: 0.34 ± 0.172
4.161AspAsp: 4.161 ± 0.68
5.265AspGlu: 5.265 ± 0.811
2.463AspPhe: 2.463 ± 0.441
4.671AspGly: 4.671 ± 0.563
0.764AspHis: 0.764 ± 0.278
3.482AspIle: 3.482 ± 0.536
4.84AspLys: 4.84 ± 0.574
6.029AspLeu: 6.029 ± 1.175
1.444AspMet: 1.444 ± 0.396
2.802AspAsn: 2.802 ± 0.461
1.783AspPro: 1.783 ± 0.46
1.953AspGln: 1.953 ± 0.409
2.717AspArg: 2.717 ± 0.575
4.076AspSer: 4.076 ± 0.648
2.717AspThr: 2.717 ± 0.511
3.397AspVal: 3.397 ± 0.452
0.849AspTrp: 0.849 ± 0.298
2.548AspTyr: 2.548 ± 0.446
0.0AspXaa: 0.0 ± 0.0
Glu
4.84GluAla: 4.84 ± 0.91
0.764GluCys: 0.764 ± 0.281
3.397GluAsp: 3.397 ± 0.413
6.454GluGlu: 6.454 ± 1.054
3.227GluPhe: 3.227 ± 0.427
4.076GluGly: 4.076 ± 0.773
0.764GluHis: 0.764 ± 0.25
5.69GluIle: 5.69 ± 0.763
8.662GluLys: 8.662 ± 0.983
8.916GluLeu: 8.916 ± 1.253
2.802GluMet: 2.802 ± 0.47
4.161GluAsn: 4.161 ± 0.546
2.548GluPro: 2.548 ± 0.457
3.227GluGln: 3.227 ± 0.535
3.312GluArg: 3.312 ± 0.527
3.651GluSer: 3.651 ± 0.503
4.161GluThr: 4.161 ± 0.787
5.944GluVal: 5.944 ± 0.69
1.019GluTrp: 1.019 ± 0.268
2.887GluTyr: 2.887 ± 0.501
0.0GluXaa: 0.0 ± 0.0
Phe
2.802PheAla: 2.802 ± 0.411
0.34PheCys: 0.34 ± 0.149
3.227PheAsp: 3.227 ± 0.647
3.057PheGlu: 3.057 ± 0.568
1.444PhePhe: 1.444 ± 0.348
2.972PheGly: 2.972 ± 0.456
0.425PheHis: 0.425 ± 0.189
2.293PheIle: 2.293 ± 0.468
2.887PheLys: 2.887 ± 0.559
2.548PheLeu: 2.548 ± 0.444
1.274PheMet: 1.274 ± 0.315
2.548PheAsn: 2.548 ± 0.385
1.019PhePro: 1.019 ± 0.293
1.189PheGln: 1.189 ± 0.281
1.189PheArg: 1.189 ± 0.326
2.463PheSer: 2.463 ± 0.514
2.293PheThr: 2.293 ± 0.414
1.868PheVal: 1.868 ± 0.31
0.425PheTrp: 0.425 ± 0.168
1.613PheTyr: 1.613 ± 0.381
0.0PheXaa: 0.0 ± 0.0
Gly
4.671GlyAla: 4.671 ± 0.826
0.255GlyCys: 0.255 ± 0.134
3.736GlyAsp: 3.736 ± 0.578
4.586GlyGlu: 4.586 ± 0.706
2.717GlyPhe: 2.717 ± 0.553
3.397GlyGly: 3.397 ± 0.614
0.849GlyHis: 0.849 ± 0.307
6.199GlyIle: 6.199 ± 0.621
4.246GlyLys: 4.246 ± 0.632
4.925GlyLeu: 4.925 ± 0.656
1.274GlyMet: 1.274 ± 0.276
3.991GlyAsn: 3.991 ± 0.458
0.679GlyPro: 0.679 ± 0.201
2.802GlyGln: 2.802 ± 0.552
1.953GlyArg: 1.953 ± 0.385
4.755GlySer: 4.755 ± 0.909
3.651GlyThr: 3.651 ± 0.929
4.671GlyVal: 4.671 ± 0.714
0.51GlyTrp: 0.51 ± 0.173
3.397GlyTyr: 3.397 ± 0.616
0.0GlyXaa: 0.0 ± 0.0
His
0.679HisAla: 0.679 ± 0.289
0.255HisCys: 0.255 ± 0.192
0.679HisAsp: 0.679 ± 0.203
0.849HisGlu: 0.849 ± 0.236
0.425HisPhe: 0.425 ± 0.187
1.189HisGly: 1.189 ± 0.298
0.17HisHis: 0.17 ± 0.127
0.51HisIle: 0.51 ± 0.193
0.849HisLys: 0.849 ± 0.239
1.019HisLeu: 1.019 ± 0.512
0.17HisMet: 0.17 ± 0.143
0.425HisAsn: 0.425 ± 0.177
0.425HisPro: 0.425 ± 0.161
0.594HisGln: 0.594 ± 0.297
0.425HisArg: 0.425 ± 0.19
0.679HisSer: 0.679 ± 0.218
0.849HisThr: 0.849 ± 0.303
1.104HisVal: 1.104 ± 0.294
0.34HisTrp: 0.34 ± 0.174
1.104HisTyr: 1.104 ± 0.358
0.0HisXaa: 0.0 ± 0.0
Ile
4.671IleAla: 4.671 ± 0.834
0.679IleCys: 0.679 ± 0.228
5.18IleAsp: 5.18 ± 0.699
6.369IleGlu: 6.369 ± 0.799
2.548IlePhe: 2.548 ± 0.488
5.01IleGly: 5.01 ± 0.918
1.019IleHis: 1.019 ± 0.249
2.717IleIle: 2.717 ± 0.49
7.812IleLys: 7.812 ± 0.766
6.114IleLeu: 6.114 ± 0.767
1.189IleMet: 1.189 ± 0.388
3.736IleAsn: 3.736 ± 0.693
2.548IlePro: 2.548 ± 0.485
2.293IleGln: 2.293 ± 0.44
2.548IleArg: 2.548 ± 0.471
4.501IleSer: 4.501 ± 0.61
4.246IleThr: 4.246 ± 0.728
3.906IleVal: 3.906 ± 0.486
0.764IleTrp: 0.764 ± 0.285
2.123IleTyr: 2.123 ± 0.493
0.0IleXaa: 0.0 ± 0.0
Lys
6.878LysAla: 6.878 ± 0.71
0.34LysCys: 0.34 ± 0.17
4.501LysAsp: 4.501 ± 0.683
9.426LysGlu: 9.426 ± 1.11
2.632LysPhe: 2.632 ± 0.424
5.265LysGly: 5.265 ± 0.882
1.613LysHis: 1.613 ± 0.416
6.454LysIle: 6.454 ± 0.764
10.615LysLys: 10.615 ± 1.446
6.284LysLeu: 6.284 ± 0.813
2.717LysMet: 2.717 ± 0.544
5.859LysAsn: 5.859 ± 0.662
3.227LysPro: 3.227 ± 0.459
4.331LysGln: 4.331 ± 0.584
3.567LysArg: 3.567 ± 0.66
5.01LysSer: 5.01 ± 0.716
5.265LysThr: 5.265 ± 0.607
5.774LysVal: 5.774 ± 0.632
1.104LysTrp: 1.104 ± 0.405
3.482LysTyr: 3.482 ± 0.761
0.0LysXaa: 0.0 ± 0.0
Leu
5.605LeuAla: 5.605 ± 0.773
0.51LeuCys: 0.51 ± 0.224
6.878LeuAsp: 6.878 ± 0.83
7.048LeuGlu: 7.048 ± 0.916
2.632LeuPhe: 2.632 ± 0.567
4.84LeuGly: 4.84 ± 0.733
0.764LeuHis: 0.764 ± 0.225
4.84LeuIle: 4.84 ± 0.727
8.152LeuLys: 8.152 ± 0.853
7.897LeuLeu: 7.897 ± 0.892
1.953LeuMet: 1.953 ± 0.446
5.35LeuAsn: 5.35 ± 0.712
2.548LeuPro: 2.548 ± 0.388
3.142LeuGln: 3.142 ± 0.626
3.397LeuArg: 3.397 ± 0.449
7.558LeuSer: 7.558 ± 0.864
5.265LeuThr: 5.265 ± 0.704
5.265LeuVal: 5.265 ± 0.771
0.849LeuTrp: 0.849 ± 0.242
2.463LeuTyr: 2.463 ± 0.481
0.0LeuXaa: 0.0 ± 0.0
Met
1.189MetAla: 1.189 ± 0.335
0.0MetCys: 0.0 ± 0.0
1.444MetAsp: 1.444 ± 0.401
1.529MetGlu: 1.529 ± 0.397
0.679MetPhe: 0.679 ± 0.245
1.613MetGly: 1.613 ± 0.308
0.085MetHis: 0.085 ± 0.076
1.868MetIle: 1.868 ± 0.396
2.378MetLys: 2.378 ± 0.434
1.698MetLeu: 1.698 ± 0.293
0.085MetMet: 0.085 ± 0.088
2.378MetAsn: 2.378 ± 0.393
0.934MetPro: 0.934 ± 0.319
0.764MetGln: 0.764 ± 0.249
1.444MetArg: 1.444 ± 0.449
2.038MetSer: 2.038 ± 0.404
1.104MetThr: 1.104 ± 0.309
1.444MetVal: 1.444 ± 0.325
0.17MetTrp: 0.17 ± 0.118
0.764MetTyr: 0.764 ± 0.309
0.0MetXaa: 0.0 ± 0.0
Asn
3.057AsnAla: 3.057 ± 0.636
0.425AsnCys: 0.425 ± 0.188
3.482AsnAsp: 3.482 ± 0.621
5.01AsnGlu: 5.01 ± 0.675
1.783AsnPhe: 1.783 ± 0.404
4.076AsnGly: 4.076 ± 0.688
0.849AsnHis: 0.849 ± 0.264
4.416AsnIle: 4.416 ± 0.708
5.52AsnLys: 5.52 ± 0.732
5.265AsnLeu: 5.265 ± 0.65
1.274AsnMet: 1.274 ± 0.259
3.142AsnAsn: 3.142 ± 0.542
2.548AsnPro: 2.548 ± 0.415
2.887AsnGln: 2.887 ± 0.411
2.378AsnArg: 2.378 ± 0.34
3.312AsnSer: 3.312 ± 0.418
2.717AsnThr: 2.717 ± 0.52
3.482AsnVal: 3.482 ± 0.504
0.764AsnTrp: 0.764 ± 0.226
2.208AsnTyr: 2.208 ± 0.335
0.0AsnXaa: 0.0 ± 0.0
Pro
2.123ProAla: 2.123 ± 0.468
0.17ProCys: 0.17 ± 0.128
2.038ProAsp: 2.038 ± 0.43
3.227ProGlu: 3.227 ± 0.618
2.293ProPhe: 2.293 ± 0.353
1.274ProGly: 1.274 ± 0.286
0.51ProHis: 0.51 ± 0.218
1.444ProIle: 1.444 ± 0.316
3.142ProLys: 3.142 ± 0.556
2.378ProLeu: 2.378 ± 0.43
0.51ProMet: 0.51 ± 0.188
2.123ProAsn: 2.123 ± 0.448
0.764ProPro: 0.764 ± 0.309
1.019ProGln: 1.019 ± 0.368
1.189ProArg: 1.189 ± 0.284
2.378ProSer: 2.378 ± 0.423
1.019ProThr: 1.019 ± 0.314
1.529ProVal: 1.529 ± 0.408
0.255ProTrp: 0.255 ± 0.123
0.679ProTyr: 0.679 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.991GlnAla: 3.991 ± 0.481
0.085GlnCys: 0.085 ± 0.098
1.953GlnAsp: 1.953 ± 0.314
2.717GlnGlu: 2.717 ± 0.526
1.613GlnPhe: 1.613 ± 0.301
2.123GlnGly: 2.123 ± 0.457
0.594GlnHis: 0.594 ± 0.221
2.887GlnIle: 2.887 ± 0.374
2.802GlnLys: 2.802 ± 0.574
3.651GlnLeu: 3.651 ± 0.527
0.849GlnMet: 0.849 ± 0.289
2.123GlnAsn: 2.123 ± 0.513
1.104GlnPro: 1.104 ± 0.305
1.359GlnGln: 1.359 ± 0.432
1.698GlnArg: 1.698 ± 0.381
2.208GlnSer: 2.208 ± 0.39
2.208GlnThr: 2.208 ± 0.448
2.548GlnVal: 2.548 ± 0.497
0.51GlnTrp: 0.51 ± 0.178
1.698GlnTyr: 1.698 ± 0.527
0.0GlnXaa: 0.0 ± 0.0
Arg
2.463ArgAla: 2.463 ± 0.407
0.17ArgCys: 0.17 ± 0.119
2.463ArgAsp: 2.463 ± 0.628
2.463ArgGlu: 2.463 ± 0.509
1.104ArgPhe: 1.104 ± 0.257
2.972ArgGly: 2.972 ± 0.59
0.425ArgHis: 0.425 ± 0.284
3.227ArgIle: 3.227 ± 0.447
4.246ArgLys: 4.246 ± 0.738
4.076ArgLeu: 4.076 ± 0.64
1.019ArgMet: 1.019 ± 0.242
2.123ArgAsn: 2.123 ± 0.537
0.679ArgPro: 0.679 ± 0.207
2.463ArgGln: 2.463 ± 0.44
1.868ArgArg: 1.868 ± 0.389
1.953ArgSer: 1.953 ± 0.53
1.359ArgThr: 1.359 ± 0.353
2.463ArgVal: 2.463 ± 0.439
0.679ArgTrp: 0.679 ± 0.242
1.444ArgTyr: 1.444 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
3.482SerAla: 3.482 ± 0.535
0.425SerCys: 0.425 ± 0.194
4.501SerAsp: 4.501 ± 0.869
4.84SerGlu: 4.84 ± 0.634
2.123SerPhe: 2.123 ± 0.491
4.84SerGly: 4.84 ± 0.759
0.934SerHis: 0.934 ± 0.334
4.925SerIle: 4.925 ± 0.602
6.114SerLys: 6.114 ± 0.714
5.69SerLeu: 5.69 ± 0.735
1.613SerMet: 1.613 ± 0.385
4.246SerAsn: 4.246 ± 0.684
1.444SerPro: 1.444 ± 0.353
2.208SerGln: 2.208 ± 0.486
2.378SerArg: 2.378 ± 0.482
5.69SerSer: 5.69 ± 1.088
2.802SerThr: 2.802 ± 0.603
3.567SerVal: 3.567 ± 0.538
0.934SerTrp: 0.934 ± 0.219
2.548SerTyr: 2.548 ± 0.37
0.0SerXaa: 0.0 ± 0.0
Thr
4.076ThrAla: 4.076 ± 0.616
0.17ThrCys: 0.17 ± 0.122
3.567ThrAsp: 3.567 ± 0.725
3.906ThrGlu: 3.906 ± 0.526
2.717ThrPhe: 2.717 ± 0.432
4.84ThrGly: 4.84 ± 0.899
0.51ThrHis: 0.51 ± 0.213
3.906ThrIle: 3.906 ± 0.736
5.01ThrLys: 5.01 ± 0.62
3.397ThrLeu: 3.397 ± 0.477
0.849ThrMet: 0.849 ± 0.297
2.463ThrAsn: 2.463 ± 0.481
2.038ThrPro: 2.038 ± 0.531
1.359ThrGln: 1.359 ± 0.321
1.444ThrArg: 1.444 ± 0.467
3.397ThrSer: 3.397 ± 0.494
2.548ThrThr: 2.548 ± 0.494
2.717ThrVal: 2.717 ± 0.377
0.764ThrTrp: 0.764 ± 0.19
2.632ThrTyr: 2.632 ± 0.467
0.0ThrXaa: 0.0 ± 0.0
Val
3.821ValAla: 3.821 ± 0.587
0.255ValCys: 0.255 ± 0.162
4.331ValAsp: 4.331 ± 0.675
4.076ValGlu: 4.076 ± 0.65
2.208ValPhe: 2.208 ± 0.471
3.142ValGly: 3.142 ± 0.526
0.849ValHis: 0.849 ± 0.26
5.435ValIle: 5.435 ± 0.703
6.284ValLys: 6.284 ± 0.63
5.095ValLeu: 5.095 ± 0.761
1.104ValMet: 1.104 ± 0.36
4.416ValAsn: 4.416 ± 0.981
1.868ValPro: 1.868 ± 0.364
2.717ValGln: 2.717 ± 0.505
2.972ValArg: 2.972 ± 0.66
3.736ValSer: 3.736 ± 0.634
3.057ValThr: 3.057 ± 0.629
4.246ValVal: 4.246 ± 0.671
0.51ValTrp: 0.51 ± 0.206
2.378ValTyr: 2.378 ± 0.433
0.0ValXaa: 0.0 ± 0.0
Trp
1.019TrpAla: 1.019 ± 0.277
0.085TrpCys: 0.085 ± 0.084
0.764TrpAsp: 0.764 ± 0.247
1.019TrpGlu: 1.019 ± 0.377
0.594TrpPhe: 0.594 ± 0.204
0.34TrpGly: 0.34 ± 0.179
0.34TrpHis: 0.34 ± 0.182
0.849TrpIle: 0.849 ± 0.248
1.274TrpLys: 1.274 ± 0.335
1.444TrpLeu: 1.444 ± 0.324
0.51TrpMet: 0.51 ± 0.165
1.189TrpAsn: 1.189 ± 0.413
0.17TrpPro: 0.17 ± 0.108
0.51TrpGln: 0.51 ± 0.203
0.594TrpArg: 0.594 ± 0.187
0.679TrpSer: 0.679 ± 0.25
0.425TrpThr: 0.425 ± 0.184
0.679TrpVal: 0.679 ± 0.215
0.0TrpTrp: 0.0 ± 0.0
0.17TrpTyr: 0.17 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.208TyrAla: 2.208 ± 0.597
0.34TyrCys: 0.34 ± 0.194
1.698TyrAsp: 1.698 ± 0.371
2.717TyrGlu: 2.717 ± 0.495
1.359TyrPhe: 1.359 ± 0.375
2.378TyrGly: 2.378 ± 0.49
0.594TyrHis: 0.594 ± 0.225
2.038TyrIle: 2.038 ± 0.446
3.057TyrLys: 3.057 ± 0.48
2.632TyrLeu: 2.632 ± 0.554
1.104TyrMet: 1.104 ± 0.36
1.868TyrAsn: 1.868 ± 0.413
2.123TyrPro: 2.123 ± 0.499
2.038TyrGln: 2.038 ± 0.448
2.208TyrArg: 2.208 ± 0.458
3.736TyrSer: 3.736 ± 0.639
1.868TyrThr: 1.868 ± 0.497
2.717TyrVal: 2.717 ± 0.621
0.594TyrTrp: 0.594 ± 0.194
1.274TyrTyr: 1.274 ± 0.421
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (11777 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski