Bermanella marisrubri
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3303 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1N0W9|Q1N0W9_9GAMM 50S ribosomal protein L24 OS=Bermanella marisrubri OX=207949 GN=rplX PE=3 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.44 KK3 pKa = 10.44 LLFAAALTAALPQAASALPLVDD25 pKa = 5.64 FYY27 pKa = 11.96 AGAHH31 pKa = 5.7 YY32 pKa = 9.76 WDD34 pKa = 3.83 QSISGEE40 pKa = 4.14 VGTGNNVADD49 pKa = 5.55 FEE51 pKa = 6.42 DD52 pKa = 5.19 DD53 pKa = 4.02 LNLDD57 pKa = 3.44 AGGQNVVYY65 pKa = 9.34 FAFEE69 pKa = 3.89 HH70 pKa = 6.81 PIPVIPNIKK79 pKa = 9.7 IKK81 pKa = 8.99 NTEE84 pKa = 3.66 MTADD88 pKa = 3.6 GNGTFASTFNLGGEE102 pKa = 4.61 TITGATGVATDD113 pKa = 5.06 LDD115 pKa = 4.56 LSHH118 pKa = 7.39 TDD120 pKa = 3.2 YY121 pKa = 10.97 TLYY124 pKa = 10.14 WGLPLPIVTLDD135 pKa = 3.33 FGITARR141 pKa = 11.84 QFDD144 pKa = 4.41 GYY146 pKa = 9.94 MRR148 pKa = 11.84 AVEE151 pKa = 4.03 NGGNSSEE158 pKa = 4.52 ADD160 pKa = 3.36 LDD162 pKa = 3.87 VTVPMGYY169 pKa = 10.39 LKK171 pKa = 10.81 AAVDD175 pKa = 3.64 IPLTGVSIGADD186 pKa = 3.53 LNAISYY192 pKa = 10.95 GDD194 pKa = 3.43 TGINDD199 pKa = 3.29 FDD201 pKa = 4.97 VNVTYY206 pKa = 10.26 VLPVIPVLDD215 pKa = 3.69 VGITAGYY222 pKa = 10.0 RR223 pKa = 11.84 SFDD226 pKa = 3.73 FEE228 pKa = 5.34 IDD230 pKa = 3.56 PEE232 pKa = 4.38 DD233 pKa = 4.17 FGGSSNDD240 pKa = 3.16 LTAEE244 pKa = 4.14 STISGPYY251 pKa = 9.82 LGLSLHH257 pKa = 6.54 LL258 pKa = 4.71
Molecular weight: 27.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.516
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.427
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.872
Patrickios 0.807
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|Q1MZ48|Q1MZ48_9GAMM Chromosome partitioning protein Soj OS=Bermanella marisrubri OX=207949 GN=RED65_08514 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 QVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.17 GRR39 pKa = 11.84 KK40 pKa = 8.14 QLSAA44 pKa = 3.9
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3303
0
3303
1063161
20
2900
321.9
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.322 ± 0.042
0.969 ± 0.014
6.0 ± 0.04
6.601 ± 0.046
4.106 ± 0.027
6.732 ± 0.044
2.348 ± 0.027
6.421 ± 0.031
5.443 ± 0.039
10.149 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.616 ± 0.022
4.332 ± 0.035
3.864 ± 0.026
4.905 ± 0.04
4.59 ± 0.027
6.59 ± 0.038
4.998 ± 0.031
6.641 ± 0.036
1.241 ± 0.02
3.131 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here