Escherichia phage GER2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Oslovirus; unclassified Oslovirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L1IVB6|A0A2L1IVB6_9CAUD Transposase OS=Escherichia phage GER2 OX=2070178 PE=4 SV=1
MM1 pKa = 7.76LNQKK5 pKa = 10.01ININVKK11 pKa = 10.4SVVTPAGTIMGEE23 pKa = 3.9VFMDD27 pKa = 4.14DD28 pKa = 4.1KK29 pKa = 11.01IIAYY33 pKa = 8.38FVVLSDD39 pKa = 3.63EE40 pKa = 4.99AISVIDD46 pKa = 3.53TEE48 pKa = 4.75GNVMFIAEE56 pKa = 4.49HH57 pKa = 6.78PEE59 pKa = 4.56DD60 pKa = 3.76IALQAAAYY68 pKa = 8.68FFAKK72 pKa = 9.82EE73 pKa = 3.91QEE75 pKa = 4.37EE76 pKa = 4.59EE77 pKa = 4.71CNCPVCQLSRR87 pKa = 11.84QINLMHH93 pKa = 7.14

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L1IV38|A0A2L1IV38_9CAUD Long tail fiber proximal subunit OS=Escherichia phage GER2 OX=2070178 PE=4 SV=1
MM1 pKa = 7.48SAATKK6 pKa = 10.24SALIRR11 pKa = 11.84TLSTVPLRR19 pKa = 11.84TEE21 pKa = 3.5EE22 pKa = 4.39RR23 pKa = 11.84YY24 pKa = 10.61SFLADD29 pKa = 3.09VVTILEE35 pKa = 4.29SQGMHH40 pKa = 5.37VASNVTVRR48 pKa = 11.84IDD50 pKa = 3.33GRR52 pKa = 11.84NFRR55 pKa = 11.84VDD57 pKa = 3.12ILATAKK63 pKa = 9.99TGGSVAIEE71 pKa = 4.15IDD73 pKa = 3.27RR74 pKa = 11.84SSPRR78 pKa = 11.84PRR80 pKa = 11.84SVMKK84 pKa = 10.54LRR86 pKa = 11.84EE87 pKa = 3.8LARR90 pKa = 11.84RR91 pKa = 11.84GTEE94 pKa = 3.93GFVLLRR100 pKa = 11.84MPKK103 pKa = 10.23KK104 pKa = 9.24LTSYY108 pKa = 10.83SDD110 pKa = 3.22AGIDD114 pKa = 4.03IIPANGKK121 pKa = 9.26GASCC125 pKa = 4.71

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

19278

37

1751

224.2

24.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.213 ± 0.338

1.141 ± 0.171

5.493 ± 0.168

6.137 ± 0.353

3.564 ± 0.182

8.149 ± 0.762

1.919 ± 0.168

5.353 ± 0.232

5.613 ± 0.255

7.962 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.173

5.094 ± 0.409

3.901 ± 0.361

4.373 ± 0.269

5.794 ± 0.42

6.666 ± 0.223

6.313 ± 0.379

6.577 ± 0.227

1.369 ± 0.111

2.796 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski