Amino acid dipepetide frequency for Escherichia phage GER2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.456AlaAla: 8.456 ± 0.995
0.882AlaCys: 0.882 ± 0.28
4.824AlaAsp: 4.824 ± 0.572
6.433AlaGlu: 6.433 ± 0.653
3.372AlaPhe: 3.372 ± 0.429
8.715AlaGly: 8.715 ± 1.182
1.349AlaHis: 1.349 ± 0.26
5.239AlaIle: 5.239 ± 0.684
4.773AlaLys: 4.773 ± 0.465
6.796AlaLeu: 6.796 ± 0.466
2.853AlaMet: 2.853 ± 0.352
3.009AlaAsn: 3.009 ± 0.431
2.905AlaPro: 2.905 ± 0.442
4.565AlaGln: 4.565 ± 0.648
5.343AlaArg: 5.343 ± 0.651
6.018AlaSer: 6.018 ± 0.58
5.81AlaThr: 5.81 ± 0.656
7.003AlaVal: 7.003 ± 0.676
1.504AlaTrp: 1.504 ± 0.268
2.283AlaTyr: 2.283 ± 0.321
0.0AlaXaa: 0.0 ± 0.0
Cys
0.83CysAla: 0.83 ± 0.213
0.156CysCys: 0.156 ± 0.081
0.623CysAsp: 0.623 ± 0.22
0.571CysGlu: 0.571 ± 0.165
0.571CysPhe: 0.571 ± 0.173
1.089CysGly: 1.089 ± 0.289
0.311CysHis: 0.311 ± 0.128
0.363CysIle: 0.363 ± 0.114
0.519CysLys: 0.519 ± 0.141
1.038CysLeu: 1.038 ± 0.24
0.208CysMet: 0.208 ± 0.121
0.519CysAsn: 0.519 ± 0.149
0.674CysPro: 0.674 ± 0.215
0.208CysGln: 0.208 ± 0.107
1.193CysArg: 1.193 ± 0.235
0.882CysSer: 0.882 ± 0.231
0.259CysThr: 0.259 ± 0.124
0.986CysVal: 0.986 ± 0.23
0.104CysTrp: 0.104 ± 0.073
0.311CysTyr: 0.311 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
5.966AspAla: 5.966 ± 0.513
0.623AspCys: 0.623 ± 0.177
3.787AspAsp: 3.787 ± 0.522
3.735AspGlu: 3.735 ± 0.562
1.816AspPhe: 1.816 ± 0.274
5.447AspGly: 5.447 ± 0.889
0.571AspHis: 0.571 ± 0.179
3.061AspIle: 3.061 ± 0.4
3.891AspLys: 3.891 ± 0.439
3.216AspLeu: 3.216 ± 0.445
1.245AspMet: 1.245 ± 0.245
3.216AspAsn: 3.216 ± 0.493
1.556AspPro: 1.556 ± 0.253
1.764AspGln: 1.764 ± 0.322
3.164AspArg: 3.164 ± 0.462
2.749AspSer: 2.749 ± 0.432
2.853AspThr: 2.853 ± 0.351
3.839AspVal: 3.839 ± 0.392
1.089AspTrp: 1.089 ± 0.233
1.349AspTyr: 1.349 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
5.343GluAla: 5.343 ± 0.648
0.934GluCys: 0.934 ± 0.258
2.075GluAsp: 2.075 ± 0.251
3.943GluGlu: 3.943 ± 0.563
2.646GluPhe: 2.646 ± 0.366
3.943GluGly: 3.943 ± 0.511
1.349GluHis: 1.349 ± 0.24
3.787GluIle: 3.787 ± 0.487
4.565GluLys: 4.565 ± 0.622
5.136GluLeu: 5.136 ± 0.545
2.127GluMet: 2.127 ± 0.344
2.698GluAsn: 2.698 ± 0.357
2.127GluPro: 2.127 ± 0.505
3.631GluGln: 3.631 ± 0.624
4.15GluArg: 4.15 ± 0.529
3.268GluSer: 3.268 ± 0.506
3.32GluThr: 3.32 ± 0.455
4.046GluVal: 4.046 ± 0.442
0.415GluTrp: 0.415 ± 0.143
1.816GluTyr: 1.816 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.801PheAla: 2.801 ± 0.407
0.726PheCys: 0.726 ± 0.172
2.127PheAsp: 2.127 ± 0.293
1.919PheGlu: 1.919 ± 0.287
1.349PhePhe: 1.349 ± 0.244
2.127PheGly: 2.127 ± 0.277
0.467PheHis: 0.467 ± 0.141
2.127PheIle: 2.127 ± 0.407
1.608PheLys: 1.608 ± 0.309
2.075PheLeu: 2.075 ± 0.285
1.141PheMet: 1.141 ± 0.234
2.334PheAsn: 2.334 ± 0.382
1.401PhePro: 1.401 ± 0.29
0.83PheGln: 0.83 ± 0.187
2.749PheArg: 2.749 ± 0.364
3.061PheSer: 3.061 ± 0.334
2.438PheThr: 2.438 ± 0.34
2.646PheVal: 2.646 ± 0.379
0.571PheTrp: 0.571 ± 0.187
1.141PheTyr: 1.141 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
7.47GlyAla: 7.47 ± 1.232
0.571GlyCys: 0.571 ± 0.207
4.513GlyAsp: 4.513 ± 0.423
5.603GlyGlu: 5.603 ± 1.132
2.749GlyPhe: 2.749 ± 0.364
6.225GlyGly: 6.225 ± 1.094
1.297GlyHis: 1.297 ± 0.304
4.876GlyIle: 4.876 ± 0.568
4.98GlyLys: 4.98 ± 0.546
5.603GlyLeu: 5.603 ± 0.589
2.438GlyMet: 2.438 ± 0.314
3.994GlyAsn: 3.994 ± 0.837
3.268GlyPro: 3.268 ± 1.888
3.372GlyGln: 3.372 ± 0.429
3.735GlyArg: 3.735 ± 0.462
5.551GlySer: 5.551 ± 1.188
5.81GlyThr: 5.81 ± 1.336
5.914GlyVal: 5.914 ± 0.604
0.674GlyTrp: 0.674 ± 0.188
2.853GlyTyr: 2.853 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.816HisAla: 1.816 ± 0.212
0.208HisCys: 0.208 ± 0.118
0.882HisAsp: 0.882 ± 0.198
0.83HisGlu: 0.83 ± 0.181
0.934HisPhe: 0.934 ± 0.232
1.297HisGly: 1.297 ± 0.26
0.519HisHis: 0.519 ± 0.18
0.882HisIle: 0.882 ± 0.183
0.934HisLys: 0.934 ± 0.213
1.868HisLeu: 1.868 ± 0.427
0.467HisMet: 0.467 ± 0.159
1.349HisAsn: 1.349 ± 0.233
1.141HisPro: 1.141 ± 0.283
0.882HisGln: 0.882 ± 0.204
0.986HisArg: 0.986 ± 0.212
1.245HisSer: 1.245 ± 0.23
0.882HisThr: 0.882 ± 0.175
1.089HisVal: 1.089 ± 0.2
0.311HisTrp: 0.311 ± 0.193
0.674HisTyr: 0.674 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
5.395IleAla: 5.395 ± 0.588
0.674IleCys: 0.674 ± 0.185
3.424IleAsp: 3.424 ± 0.394
3.113IleGlu: 3.113 ± 0.426
1.297IlePhe: 1.297 ± 0.274
3.061IleGly: 3.061 ± 0.469
1.089IleHis: 1.089 ± 0.224
2.853IleIle: 2.853 ± 0.323
3.268IleLys: 3.268 ± 0.339
3.735IleLeu: 3.735 ± 0.42
0.934IleMet: 0.934 ± 0.227
3.787IleAsn: 3.787 ± 0.757
2.646IlePro: 2.646 ± 0.335
2.231IleGln: 2.231 ± 0.289
3.891IleArg: 3.891 ± 0.407
4.098IleSer: 4.098 ± 0.462
3.891IleThr: 3.891 ± 0.54
2.542IleVal: 2.542 ± 0.278
0.363IleTrp: 0.363 ± 0.14
1.245IleTyr: 1.245 ± 0.247
0.0IleXaa: 0.0 ± 0.0
Lys
5.654LysAla: 5.654 ± 0.584
0.726LysCys: 0.726 ± 0.185
2.542LysAsp: 2.542 ± 0.287
3.268LysGlu: 3.268 ± 0.413
1.453LysPhe: 1.453 ± 0.321
4.306LysGly: 4.306 ± 0.729
1.712LysHis: 1.712 ± 0.325
3.113LysIle: 3.113 ± 0.39
3.579LysLys: 3.579 ± 0.521
4.046LysLeu: 4.046 ± 0.499
1.193LysMet: 1.193 ± 0.263
3.476LysAsn: 3.476 ± 0.427
2.698LysPro: 2.698 ± 0.424
3.164LysGln: 3.164 ± 0.369
2.231LysArg: 2.231 ± 0.325
3.631LysSer: 3.631 ± 0.491
3.891LysThr: 3.891 ± 0.488
3.216LysVal: 3.216 ± 0.357
0.674LysTrp: 0.674 ± 0.163
1.556LysTyr: 1.556 ± 0.28
0.0LysXaa: 0.0 ± 0.0
Leu
7.678LeuAla: 7.678 ± 0.619
0.83LeuCys: 0.83 ± 0.183
4.202LeuAsp: 4.202 ± 0.516
3.943LeuGlu: 3.943 ± 0.437
2.646LeuPhe: 2.646 ± 0.41
5.084LeuGly: 5.084 ± 0.535
1.712LeuHis: 1.712 ± 0.314
4.254LeuIle: 4.254 ± 0.517
4.046LeuLys: 4.046 ± 0.523
6.692LeuLeu: 6.692 ± 0.679
1.971LeuMet: 1.971 ± 0.363
4.358LeuAsn: 4.358 ± 0.443
3.631LeuPro: 3.631 ± 0.584
4.565LeuGln: 4.565 ± 0.524
3.994LeuArg: 3.994 ± 0.522
7.055LeuSer: 7.055 ± 0.653
5.706LeuThr: 5.706 ± 0.543
4.669LeuVal: 4.669 ± 0.437
0.571LeuTrp: 0.571 ± 0.154
2.023LeuTyr: 2.023 ± 0.343
0.0LeuXaa: 0.0 ± 0.0
Met
2.853MetAla: 2.853 ± 0.388
0.156MetCys: 0.156 ± 0.095
1.297MetAsp: 1.297 ± 0.206
1.401MetGlu: 1.401 ± 0.299
0.778MetPhe: 0.778 ± 0.198
1.297MetGly: 1.297 ± 0.266
0.623MetHis: 0.623 ± 0.202
0.778MetIle: 0.778 ± 0.188
1.919MetLys: 1.919 ± 0.308
1.971MetLeu: 1.971 ± 0.28
0.726MetMet: 0.726 ± 0.255
1.712MetAsn: 1.712 ± 0.365
1.193MetPro: 1.193 ± 0.18
0.986MetGln: 0.986 ± 0.169
1.608MetArg: 1.608 ± 0.307
1.712MetSer: 1.712 ± 0.209
2.49MetThr: 2.49 ± 0.3
1.297MetVal: 1.297 ± 0.262
0.311MetTrp: 0.311 ± 0.117
0.623MetTyr: 0.623 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
5.084AsnAla: 5.084 ± 0.496
0.519AsnCys: 0.519 ± 0.131
2.698AsnAsp: 2.698 ± 0.364
2.438AsnGlu: 2.438 ± 0.307
1.66AsnPhe: 1.66 ± 0.327
4.669AsnGly: 4.669 ± 0.795
1.453AsnHis: 1.453 ± 0.238
3.528AsnIle: 3.528 ± 0.521
3.009AsnLys: 3.009 ± 0.427
3.631AsnLeu: 3.631 ± 0.447
1.193AsnMet: 1.193 ± 0.262
3.476AsnAsn: 3.476 ± 0.59
1.764AsnPro: 1.764 ± 0.309
1.764AsnGln: 1.764 ± 0.276
2.075AsnArg: 2.075 ± 0.372
3.216AsnSer: 3.216 ± 0.536
3.009AsnThr: 3.009 ± 0.639
3.579AsnVal: 3.579 ± 0.67
0.726AsnTrp: 0.726 ± 0.233
1.453AsnTyr: 1.453 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
3.994ProAla: 3.994 ± 0.739
0.519ProCys: 0.519 ± 0.171
3.216ProAsp: 3.216 ± 0.434
3.735ProGlu: 3.735 ± 0.552
1.401ProPhe: 1.401 ± 0.242
3.216ProGly: 3.216 ± 0.676
0.674ProHis: 0.674 ± 0.208
0.674ProIle: 0.674 ± 0.205
2.075ProLys: 2.075 ± 0.445
2.749ProLeu: 2.749 ± 0.422
0.311ProMet: 0.311 ± 0.116
0.882ProAsn: 0.882 ± 0.223
1.089ProPro: 1.089 ± 0.23
1.868ProGln: 1.868 ± 0.481
2.386ProArg: 2.386 ± 0.498
3.009ProSer: 3.009 ± 0.41
1.453ProThr: 1.453 ± 0.305
3.528ProVal: 3.528 ± 0.598
0.623ProTrp: 0.623 ± 0.161
1.608ProTyr: 1.608 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
3.735GlnAla: 3.735 ± 0.522
0.623GlnCys: 0.623 ± 0.186
2.179GlnAsp: 2.179 ± 0.3
2.334GlnGlu: 2.334 ± 0.51
1.297GlnPhe: 1.297 ± 0.214
3.891GlnGly: 3.891 ± 0.646
0.882GlnHis: 0.882 ± 0.226
2.386GlnIle: 2.386 ± 0.339
3.32GlnLys: 3.32 ± 0.408
4.306GlnLeu: 4.306 ± 0.5
0.778GlnMet: 0.778 ± 0.246
2.127GlnAsn: 2.127 ± 0.342
1.712GlnPro: 1.712 ± 0.497
2.698GlnGln: 2.698 ± 0.75
2.957GlnArg: 2.957 ± 0.543
2.594GlnSer: 2.594 ± 0.277
1.919GlnThr: 1.919 ± 0.285
2.127GlnVal: 2.127 ± 0.451
0.726GlnTrp: 0.726 ± 0.176
1.141GlnTyr: 1.141 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
3.994ArgAla: 3.994 ± 0.477
0.674ArgCys: 0.674 ± 0.206
2.698ArgAsp: 2.698 ± 0.46
4.098ArgGlu: 4.098 ± 0.564
3.009ArgPhe: 3.009 ± 0.443
4.565ArgGly: 4.565 ± 0.825
1.504ArgHis: 1.504 ± 0.247
3.32ArgIle: 3.32 ± 0.338
3.476ArgLys: 3.476 ± 0.502
5.084ArgLeu: 5.084 ± 0.525
1.349ArgMet: 1.349 ± 0.312
3.009ArgAsn: 3.009 ± 0.381
1.868ArgPro: 1.868 ± 0.275
2.646ArgGln: 2.646 ± 0.361
4.15ArgArg: 4.15 ± 0.652
3.476ArgSer: 3.476 ± 0.443
2.801ArgThr: 2.801 ± 0.383
3.164ArgVal: 3.164 ± 0.542
1.245ArgTrp: 1.245 ± 0.244
1.816ArgTyr: 1.816 ± 0.302
0.0ArgXaa: 0.0 ± 0.0
Ser
6.329SerAla: 6.329 ± 0.683
0.83SerCys: 0.83 ± 0.198
4.046SerAsp: 4.046 ± 0.407
4.098SerGlu: 4.098 ± 0.408
2.283SerPhe: 2.283 ± 0.319
7.626SerGly: 7.626 ± 1.095
1.141SerHis: 1.141 ± 0.255
2.49SerIle: 2.49 ± 0.27
2.853SerLys: 2.853 ± 0.417
6.329SerLeu: 6.329 ± 0.607
1.971SerMet: 1.971 ± 0.318
2.386SerAsn: 2.386 ± 0.389
3.009SerPro: 3.009 ± 0.403
2.594SerGln: 2.594 ± 0.338
3.994SerArg: 3.994 ± 0.439
3.424SerSer: 3.424 ± 0.418
3.994SerThr: 3.994 ± 0.459
4.721SerVal: 4.721 ± 0.513
0.934SerTrp: 0.934 ± 0.203
1.608SerTyr: 1.608 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
6.329ThrAla: 6.329 ± 0.585
0.363ThrCys: 0.363 ± 0.145
3.32ThrAsp: 3.32 ± 0.37
3.994ThrGlu: 3.994 ± 0.435
2.49ThrPhe: 2.49 ± 0.329
7.159ThrGly: 7.159 ± 1.119
1.089ThrHis: 1.089 ± 0.21
3.735ThrIle: 3.735 ± 0.739
2.386ThrLys: 2.386 ± 0.342
5.966ThrLeu: 5.966 ± 0.634
1.349ThrMet: 1.349 ± 0.228
2.127ThrAsn: 2.127 ± 0.498
2.957ThrPro: 2.957 ± 0.375
2.075ThrGln: 2.075 ± 0.366
2.646ThrArg: 2.646 ± 0.295
3.943ThrSer: 3.943 ± 0.416
4.306ThrThr: 4.306 ± 0.471
4.306ThrVal: 4.306 ± 0.541
0.986ThrTrp: 0.986 ± 0.23
1.608ThrTyr: 1.608 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
5.551ValAla: 5.551 ± 0.49
1.038ValCys: 1.038 ± 0.312
3.943ValAsp: 3.943 ± 0.483
3.683ValGlu: 3.683 ± 0.302
2.179ValPhe: 2.179 ± 0.324
4.358ValGly: 4.358 ± 0.605
0.83ValHis: 0.83 ± 0.209
3.528ValIle: 3.528 ± 0.408
3.268ValLys: 3.268 ± 0.448
5.966ValLeu: 5.966 ± 0.608
2.127ValMet: 2.127 ± 0.277
4.15ValAsn: 4.15 ± 0.592
1.971ValPro: 1.971 ± 0.321
2.231ValGln: 2.231 ± 0.426
3.372ValArg: 3.372 ± 0.351
4.928ValSer: 4.928 ± 0.617
5.447ValThr: 5.447 ± 0.52
4.565ValVal: 4.565 ± 0.546
1.038ValTrp: 1.038 ± 0.232
1.608ValTyr: 1.608 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
0.778TrpAla: 0.778 ± 0.17
0.259TrpCys: 0.259 ± 0.107
0.726TrpAsp: 0.726 ± 0.215
0.623TrpGlu: 0.623 ± 0.178
0.519TrpPhe: 0.519 ± 0.139
0.986TrpGly: 0.986 ± 0.23
0.311TrpHis: 0.311 ± 0.123
0.778TrpIle: 0.778 ± 0.195
0.674TrpLys: 0.674 ± 0.166
1.453TrpLeu: 1.453 ± 0.311
0.571TrpMet: 0.571 ± 0.166
0.623TrpAsn: 0.623 ± 0.178
0.467TrpPro: 0.467 ± 0.161
0.726TrpGln: 0.726 ± 0.186
1.193TrpArg: 1.193 ± 0.216
0.986TrpSer: 0.986 ± 0.216
0.674TrpThr: 0.674 ± 0.187
0.674TrpVal: 0.674 ± 0.171
0.415TrpTrp: 0.415 ± 0.154
0.259TrpTyr: 0.259 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.075TyrAla: 2.075 ± 0.338
0.104TyrCys: 0.104 ± 0.069
1.816TyrAsp: 1.816 ± 0.331
1.608TyrGlu: 1.608 ± 0.329
1.193TyrPhe: 1.193 ± 0.228
2.438TyrGly: 2.438 ± 0.395
0.311TyrHis: 0.311 ± 0.12
1.764TyrIle: 1.764 ± 0.292
0.986TyrLys: 0.986 ± 0.178
1.971TyrLeu: 1.971 ± 0.324
0.726TyrMet: 0.726 ± 0.198
1.712TyrAsn: 1.712 ± 0.283
0.934TyrPro: 0.934 ± 0.265
0.934TyrGln: 0.934 ± 0.165
2.127TyrArg: 2.127 ± 0.352
1.816TyrSer: 1.816 ± 0.304
2.179TyrThr: 2.179 ± 0.405
1.868TyrVal: 1.868 ± 0.244
0.415TyrTrp: 0.415 ± 0.148
0.986TyrTyr: 0.986 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (19278 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski