Alistipes sp. AF14-19

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; unclassified Alistipes

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373ZUV4|A0A373ZUV4_9BACT PspC domain-containing protein OS=Alistipes sp. AF14-19 OX=2292910 GN=DWW03_14150 PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 5.64AKK4 pKa = 10.07VLSEE8 pKa = 3.7AKK10 pKa = 10.47VYY12 pKa = 10.65VGTYY16 pKa = 9.99AKK18 pKa = 10.34YY19 pKa = 10.91NNGSLSGAWLDD30 pKa = 4.44LSDD33 pKa = 4.65YY34 pKa = 10.83SDD36 pKa = 3.73KK37 pKa = 11.66EE38 pKa = 4.09EE39 pKa = 4.45FYY41 pKa = 9.88EE42 pKa = 4.99ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 6.63KK49 pKa = 11.13DD50 pKa = 3.63EE51 pKa = 5.18EE52 pKa = 4.44DD53 pKa = 3.48AEE55 pKa = 4.74YY56 pKa = 10.09MFQDD60 pKa = 3.69WEE62 pKa = 4.32NVPEE66 pKa = 4.11GLIGEE71 pKa = 4.39SWISEE76 pKa = 3.98NFFALRR82 pKa = 11.84DD83 pKa = 3.86AVEE86 pKa = 4.69DD87 pKa = 4.15LSDD90 pKa = 3.72TEE92 pKa = 4.18QEE94 pKa = 5.68AFFVWCNYY102 pKa = 9.32KK103 pKa = 10.32SHH105 pKa = 7.73DD106 pKa = 4.59LGEE109 pKa = 4.33EE110 pKa = 3.98DD111 pKa = 5.6ADD113 pKa = 4.0DD114 pKa = 3.91LVRR117 pKa = 11.84DD118 pKa = 4.29FRR120 pKa = 11.84DD121 pKa = 3.62EE122 pKa = 4.06YY123 pKa = 11.0LGQYY127 pKa = 10.61DD128 pKa = 4.25DD129 pKa = 5.37EE130 pKa = 5.2EE131 pKa = 5.71DD132 pKa = 3.22FAYY135 pKa = 10.08EE136 pKa = 4.43IIEE139 pKa = 4.11EE140 pKa = 4.66CYY142 pKa = 10.66DD143 pKa = 3.52LPEE146 pKa = 4.02FAKK149 pKa = 10.18TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 5.04LFMCDD165 pKa = 2.54YY166 pKa = 9.94WFDD169 pKa = 3.78DD170 pKa = 3.9GFVFRR175 pKa = 11.84AAA177 pKa = 3.87

Molecular weight: 21.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A374A2R5|A0A374A2R5_9BACT ATP-grasp domain-containing protein OS=Alistipes sp. AF14-19 OX=2292910 GN=DWW03_09330 PE=4 SV=1
MM1 pKa = 7.3LHH3 pKa = 6.72LSKK6 pKa = 10.75HH7 pKa = 5.24RR8 pKa = 11.84LGVRR12 pKa = 11.84QCPFLQLCGSRR23 pKa = 11.84HH24 pKa = 4.3TFVPNSFWAMVCRR37 pKa = 11.84RR38 pKa = 11.84TIPLLRR44 pKa = 11.84KK45 pKa = 9.14ILMNEE50 pKa = 4.52GIKK53 pKa = 10.74NGSLYY58 pKa = 10.88QNLEE62 pKa = 4.19LNSYY66 pKa = 8.75FCIRR70 pKa = 11.84RR71 pKa = 11.84VLL73 pKa = 3.48

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3142

0

3142

1073081

26

2482

341.5

38.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 0.054

1.301 ± 0.017

5.736 ± 0.033

6.623 ± 0.047

4.279 ± 0.028

7.355 ± 0.041

1.755 ± 0.018

5.867 ± 0.036

5.161 ± 0.043

8.92 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.019

4.074 ± 0.033

4.066 ± 0.025

3.08 ± 0.025

6.427 ± 0.048

5.777 ± 0.036

5.753 ± 0.032

6.884 ± 0.032

1.284 ± 0.017

4.085 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski