Amino acid dipepetide frequency for Alistipes sp. AF14-19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.814AlaAla: 10.814 ± 0.151
1.221AlaCys: 1.221 ± 0.035
5.475AlaAsp: 5.475 ± 0.079
6.815AlaGlu: 6.815 ± 0.097
3.528AlaPhe: 3.528 ± 0.066
7.424AlaGly: 7.424 ± 0.106
1.356AlaHis: 1.356 ± 0.035
4.344AlaIle: 4.344 ± 0.062
3.927AlaLys: 3.927 ± 0.075
8.122AlaLeu: 8.122 ± 0.107
2.239AlaMet: 2.239 ± 0.049
2.912AlaAsn: 2.912 ± 0.055
3.318AlaPro: 3.318 ± 0.062
2.997AlaGln: 2.997 ± 0.064
5.142AlaArg: 5.142 ± 0.089
4.947AlaSer: 4.947 ± 0.07
4.573AlaThr: 4.573 ± 0.071
6.949AlaVal: 6.949 ± 0.096
1.129AlaTrp: 1.129 ± 0.033
3.07AlaTyr: 3.07 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
1.033CysAla: 1.033 ± 0.036
0.24CysCys: 0.24 ± 0.015
0.815CysAsp: 0.815 ± 0.028
0.773CysGlu: 0.773 ± 0.03
0.517CysPhe: 0.517 ± 0.023
1.225CysGly: 1.225 ± 0.038
0.264CysHis: 0.264 ± 0.015
0.756CysIle: 0.756 ± 0.026
0.566CysLys: 0.566 ± 0.025
0.993CysLeu: 0.993 ± 0.035
0.281CysMet: 0.281 ± 0.017
0.548CysAsn: 0.548 ± 0.024
0.623CysPro: 0.623 ± 0.026
0.275CysGln: 0.275 ± 0.016
1.065CysArg: 1.065 ± 0.038
0.794CysSer: 0.794 ± 0.027
0.732CysThr: 0.732 ± 0.028
0.795CysVal: 0.795 ± 0.031
0.157CysTrp: 0.157 ± 0.012
0.556CysTyr: 0.556 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.143AspAla: 5.143 ± 0.076
0.714AspCys: 0.714 ± 0.031
3.199AspAsp: 3.199 ± 0.066
3.862AspGlu: 3.862 ± 0.062
2.907AspPhe: 2.907 ± 0.051
4.837AspGly: 4.837 ± 0.078
0.861AspHis: 0.861 ± 0.031
3.683AspIle: 3.683 ± 0.055
3.194AspLys: 3.194 ± 0.06
4.667AspLeu: 4.667 ± 0.068
1.468AspMet: 1.468 ± 0.037
2.397AspAsn: 2.397 ± 0.053
2.621AspPro: 2.621 ± 0.048
1.143AspGln: 1.143 ± 0.038
3.659AspArg: 3.659 ± 0.062
2.949AspSer: 2.949 ± 0.058
2.883AspThr: 2.883 ± 0.055
3.575AspVal: 3.575 ± 0.067
0.898AspTrp: 0.898 ± 0.03
2.7AspTyr: 2.7 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.907GluAla: 5.907 ± 0.097
0.763GluCys: 0.763 ± 0.024
2.896GluAsp: 2.896 ± 0.055
4.767GluGlu: 4.767 ± 0.08
2.606GluPhe: 2.606 ± 0.049
4.439GluGly: 4.439 ± 0.068
1.229GluHis: 1.229 ± 0.036
4.426GluIle: 4.426 ± 0.071
4.144GluLys: 4.144 ± 0.066
6.024GluLeu: 6.024 ± 0.075
1.99GluMet: 1.99 ± 0.046
3.022GluAsn: 3.022 ± 0.05
2.121GluPro: 2.121 ± 0.041
2.581GluGln: 2.581 ± 0.057
4.678GluArg: 4.678 ± 0.076
3.194GluSer: 3.194 ± 0.052
3.554GluThr: 3.554 ± 0.055
4.307GluVal: 4.307 ± 0.074
0.889GluTrp: 0.889 ± 0.03
2.695GluTyr: 2.695 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.793PheAla: 3.793 ± 0.069
0.58PheCys: 0.58 ± 0.026
2.975PheAsp: 2.975 ± 0.056
2.54PheGlu: 2.54 ± 0.044
1.871PhePhe: 1.871 ± 0.053
3.414PheGly: 3.414 ± 0.069
0.755PheHis: 0.755 ± 0.026
2.362PheIle: 2.362 ± 0.058
1.772PheLys: 1.772 ± 0.044
3.41PheLeu: 3.41 ± 0.065
0.997PheMet: 0.997 ± 0.03
1.835PheAsn: 1.835 ± 0.045
1.724PhePro: 1.724 ± 0.045
0.986PheGln: 0.986 ± 0.033
2.872PheArg: 2.872 ± 0.052
3.043PheSer: 3.043 ± 0.054
2.735PheThr: 2.735 ± 0.052
2.831PheVal: 2.831 ± 0.05
0.534PheTrp: 0.534 ± 0.026
1.761PheTyr: 1.761 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
5.814GlyAla: 5.814 ± 0.092
1.123GlyCys: 1.123 ± 0.038
4.124GlyAsp: 4.124 ± 0.065
4.825GlyGlu: 4.825 ± 0.071
3.189GlyPhe: 3.189 ± 0.058
5.639GlyGly: 5.639 ± 0.104
1.338GlyHis: 1.338 ± 0.038
4.742GlyIle: 4.742 ± 0.072
4.341GlyLys: 4.341 ± 0.067
6.003GlyLeu: 6.003 ± 0.086
2.021GlyMet: 2.021 ± 0.055
3.1GlyAsn: 3.1 ± 0.065
1.512GlyPro: 1.512 ± 0.038
2.149GlyGln: 2.149 ± 0.049
4.892GlyArg: 4.892 ± 0.087
4.322GlySer: 4.322 ± 0.068
4.357GlyThr: 4.357 ± 0.069
5.509GlyVal: 5.509 ± 0.081
1.088GlyTrp: 1.088 ± 0.036
3.467GlyTyr: 3.467 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.309HisAla: 1.309 ± 0.031
0.275HisCys: 0.275 ± 0.015
0.954HisAsp: 0.954 ± 0.031
0.972HisGlu: 0.972 ± 0.031
0.855HisPhe: 0.855 ± 0.032
1.289HisGly: 1.289 ± 0.033
0.37HisHis: 0.37 ± 0.02
1.254HisIle: 1.254 ± 0.034
0.842HisLys: 0.842 ± 0.028
1.598HisLeu: 1.598 ± 0.038
0.355HisMet: 0.355 ± 0.018
0.798HisAsn: 0.798 ± 0.026
1.019HisPro: 1.019 ± 0.036
0.448HisGln: 0.448 ± 0.02
1.111HisArg: 1.111 ± 0.032
0.91HisSer: 0.91 ± 0.031
1.104HisThr: 1.104 ± 0.034
1.036HisVal: 1.036 ± 0.034
0.218HisTrp: 0.218 ± 0.015
0.828HisTyr: 0.828 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.686IleAla: 5.686 ± 0.075
0.811IleCys: 0.811 ± 0.029
3.961IleAsp: 3.961 ± 0.057
4.002IleGlu: 4.002 ± 0.07
2.304IlePhe: 2.304 ± 0.058
4.337IleGly: 4.337 ± 0.071
1.06IleHis: 1.06 ± 0.032
3.073IleIle: 3.073 ± 0.066
2.546IleLys: 2.546 ± 0.057
4.912IleLeu: 4.912 ± 0.088
1.122IleMet: 1.122 ± 0.035
2.295IleAsn: 2.295 ± 0.05
2.912IlePro: 2.912 ± 0.057
1.46IleGln: 1.46 ± 0.039
4.176IleArg: 4.176 ± 0.071
3.617IleSer: 3.617 ± 0.062
3.392IleThr: 3.392 ± 0.057
4.239IleVal: 4.239 ± 0.064
0.544IleTrp: 0.544 ± 0.026
2.223IleTyr: 2.223 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.386LysAla: 4.386 ± 0.08
0.492LysCys: 0.492 ± 0.024
2.676LysAsp: 2.676 ± 0.06
3.666LysGlu: 3.666 ± 0.066
1.868LysPhe: 1.868 ± 0.046
3.519LysGly: 3.519 ± 0.07
0.904LysHis: 0.904 ± 0.028
3.495LysIle: 3.495 ± 0.058
3.101LysLys: 3.101 ± 0.069
4.229LysLeu: 4.229 ± 0.066
1.69LysMet: 1.69 ± 0.043
2.266LysAsn: 2.266 ± 0.053
2.018LysPro: 2.018 ± 0.041
1.826LysGln: 1.826 ± 0.047
3.054LysArg: 3.054 ± 0.056
2.825LysSer: 2.825 ± 0.053
3.164LysThr: 3.164 ± 0.054
3.472LysVal: 3.472 ± 0.059
0.651LysTrp: 0.651 ± 0.025
2.303LysTyr: 2.303 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
8.174LeuAla: 8.174 ± 0.112
1.417LeuCys: 1.417 ± 0.038
4.841LeuAsp: 4.841 ± 0.068
5.097LeuGlu: 5.097 ± 0.072
4.207LeuPhe: 4.207 ± 0.076
5.942LeuGly: 5.942 ± 0.081
1.69LeuHis: 1.69 ± 0.041
4.473LeuIle: 4.473 ± 0.082
4.848LeuLys: 4.848 ± 0.067
8.679LeuLeu: 8.679 ± 0.119
2.237LeuMet: 2.237 ± 0.046
3.552LeuAsn: 3.552 ± 0.068
4.033LeuPro: 4.033 ± 0.063
2.744LeuGln: 2.744 ± 0.057
6.161LeuArg: 6.161 ± 0.079
5.975LeuSer: 5.975 ± 0.082
5.508LeuThr: 5.508 ± 0.068
5.275LeuVal: 5.275 ± 0.08
1.094LeuTrp: 1.094 ± 0.037
3.254LeuTyr: 3.254 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.275MetAla: 2.275 ± 0.052
0.264MetCys: 0.264 ± 0.016
1.29MetAsp: 1.29 ± 0.032
1.636MetGlu: 1.636 ± 0.041
0.884MetPhe: 0.884 ± 0.026
1.537MetGly: 1.537 ± 0.043
0.445MetHis: 0.445 ± 0.019
1.348MetIle: 1.348 ± 0.039
1.978MetLys: 1.978 ± 0.041
2.51MetLeu: 2.51 ± 0.056
0.684MetMet: 0.684 ± 0.027
1.189MetAsn: 1.189 ± 0.034
1.234MetPro: 1.234 ± 0.033
1.037MetGln: 1.037 ± 0.033
1.678MetArg: 1.678 ± 0.041
1.454MetSer: 1.454 ± 0.037
1.563MetThr: 1.563 ± 0.039
1.413MetVal: 1.413 ± 0.037
0.278MetTrp: 0.278 ± 0.016
0.736MetTyr: 0.736 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.394AsnAla: 3.394 ± 0.054
0.474AsnCys: 0.474 ± 0.025
2.195AsnAsp: 2.195 ± 0.053
2.211AsnGlu: 2.211 ± 0.055
1.787AsnPhe: 1.787 ± 0.037
3.294AsnGly: 3.294 ± 0.058
0.692AsnHis: 0.692 ± 0.025
2.817AsnIle: 2.817 ± 0.052
1.953AsnLys: 1.953 ± 0.047
3.524AsnLeu: 3.524 ± 0.063
1.023AsnMet: 1.023 ± 0.033
1.846AsnAsn: 1.846 ± 0.043
2.348AsnPro: 2.348 ± 0.044
1.038AsnGln: 1.038 ± 0.03
2.617AsnArg: 2.617 ± 0.047
2.263AsnSer: 2.263 ± 0.048
2.215AsnThr: 2.215 ± 0.047
2.642AsnVal: 2.642 ± 0.054
0.493AsnTrp: 0.493 ± 0.023
1.915AsnTyr: 1.915 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
4.46ProAla: 4.46 ± 0.074
0.44ProCys: 0.44 ± 0.021
2.943ProAsp: 2.943 ± 0.06
3.718ProGlu: 3.718 ± 0.061
1.707ProPhe: 1.707 ± 0.042
2.951ProGly: 2.951 ± 0.06
0.698ProHis: 0.698 ± 0.025
1.933ProIle: 1.933 ± 0.047
1.888ProLys: 1.888 ± 0.042
3.443ProLeu: 3.443 ± 0.061
0.927ProMet: 0.927 ± 0.033
1.348ProAsn: 1.348 ± 0.038
1.039ProPro: 1.039 ± 0.035
1.46ProGln: 1.46 ± 0.039
2.023ProArg: 2.023 ± 0.043
2.306ProSer: 2.306 ± 0.037
1.966ProThr: 1.966 ± 0.045
3.31ProVal: 3.31 ± 0.049
0.463ProTrp: 0.463 ± 0.022
1.634ProTyr: 1.634 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.547GlnAla: 2.547 ± 0.048
0.291GlnCys: 0.291 ± 0.016
1.308GlnAsp: 1.308 ± 0.04
1.87GlnGlu: 1.87 ± 0.049
1.184GlnPhe: 1.184 ± 0.035
1.928GlnGly: 1.928 ± 0.043
0.564GlnHis: 0.564 ± 0.023
2.078GlnIle: 2.078 ± 0.049
1.739GlnLys: 1.739 ± 0.042
2.874GlnLeu: 2.874 ± 0.062
0.879GlnMet: 0.879 ± 0.03
1.422GlnAsn: 1.422 ± 0.044
1.323GlnPro: 1.323 ± 0.041
1.421GlnGln: 1.421 ± 0.048
2.092GlnArg: 2.092 ± 0.05
1.802GlnSer: 1.802 ± 0.042
1.975GlnThr: 1.975 ± 0.049
1.81GlnVal: 1.81 ± 0.044
0.445GlnTrp: 0.445 ± 0.02
1.245GlnTyr: 1.245 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
4.587ArgAla: 4.587 ± 0.079
0.749ArgCys: 0.749 ± 0.027
3.195ArgAsp: 3.195 ± 0.061
4.687ArgGlu: 4.687 ± 0.074
2.924ArgPhe: 2.924 ± 0.052
3.651ArgGly: 3.651 ± 0.073
1.376ArgHis: 1.376 ± 0.041
4.609ArgIle: 4.609 ± 0.072
3.457ArgLys: 3.457 ± 0.061
6.132ArgLeu: 6.132 ± 0.087
1.929ArgMet: 1.929 ± 0.045
2.834ArgAsn: 2.834 ± 0.057
2.425ArgPro: 2.425 ± 0.045
2.367ArgGln: 2.367 ± 0.051
4.84ArgArg: 4.84 ± 0.099
3.394ArgSer: 3.394 ± 0.06
3.742ArgThr: 3.742 ± 0.061
3.668ArgVal: 3.668 ± 0.062
0.867ArgTrp: 0.867 ± 0.028
2.838ArgTyr: 2.838 ± 0.052
0.001ArgXaa: 0.001 ± 0.001
Ser
5.03SerAla: 5.03 ± 0.073
0.795SerCys: 0.795 ± 0.025
3.433SerAsp: 3.433 ± 0.066
3.565SerGlu: 3.565 ± 0.061
2.726SerPhe: 2.726 ± 0.055
4.799SerGly: 4.799 ± 0.071
0.993SerHis: 0.993 ± 0.032
3.216SerIle: 3.216 ± 0.068
2.715SerLys: 2.715 ± 0.054
5.615SerLeu: 5.615 ± 0.08
1.322SerMet: 1.322 ± 0.038
2.076SerAsn: 2.076 ± 0.045
2.349SerPro: 2.349 ± 0.051
1.737SerGln: 1.737 ± 0.049
3.222SerArg: 3.222 ± 0.064
3.336SerSer: 3.336 ± 0.074
2.963SerThr: 2.963 ± 0.051
4.561SerVal: 4.561 ± 0.072
0.767SerTrp: 0.767 ± 0.03
2.545SerTyr: 2.545 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.436ThrAla: 5.436 ± 0.081
0.551ThrCys: 0.551 ± 0.025
3.643ThrAsp: 3.643 ± 0.06
3.454ThrGlu: 3.454 ± 0.06
2.511ThrPhe: 2.511 ± 0.052
4.577ThrGly: 4.577 ± 0.066
0.965ThrHis: 0.965 ± 0.033
3.201ThrIle: 3.201 ± 0.065
2.321ThrLys: 2.321 ± 0.049
5.85ThrLeu: 5.85 ± 0.076
1.211ThrMet: 1.211 ± 0.031
1.895ThrAsn: 1.895 ± 0.045
3.195ThrPro: 3.195 ± 0.052
1.697ThrGln: 1.697 ± 0.042
2.901ThrArg: 2.901 ± 0.05
3.056ThrSer: 3.056 ± 0.068
3.469ThrThr: 3.469 ± 0.074
4.804ThrVal: 4.804 ± 0.08
0.653ThrTrp: 0.653 ± 0.027
2.14ThrTyr: 2.14 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
6.179ValAla: 6.179 ± 0.098
1.052ValCys: 1.052 ± 0.034
3.923ValAsp: 3.923 ± 0.067
4.625ValGlu: 4.625 ± 0.066
2.789ValPhe: 2.789 ± 0.053
4.646ValGly: 4.646 ± 0.077
1.003ValHis: 1.003 ± 0.031
3.836ValIle: 3.836 ± 0.056
3.632ValLys: 3.632 ± 0.062
5.779ValLeu: 5.779 ± 0.09
1.697ValMet: 1.697 ± 0.042
2.729ValAsn: 2.729 ± 0.066
2.869ValPro: 2.869 ± 0.05
1.918ValGln: 1.918 ± 0.037
4.556ValArg: 4.556 ± 0.071
4.484ValSer: 4.484 ± 0.067
4.276ValThr: 4.276 ± 0.08
5.394ValVal: 5.394 ± 0.086
0.941ValTrp: 0.941 ± 0.036
2.511ValTyr: 2.511 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.877TrpAla: 0.877 ± 0.034
0.217TrpCys: 0.217 ± 0.013
0.706TrpAsp: 0.706 ± 0.026
0.777TrpGlu: 0.777 ± 0.025
0.64TrpPhe: 0.64 ± 0.025
0.969TrpGly: 0.969 ± 0.033
0.272TrpHis: 0.272 ± 0.017
0.771TrpIle: 0.771 ± 0.028
0.658TrpLys: 0.658 ± 0.026
1.273TrpLeu: 1.273 ± 0.036
0.393TrpMet: 0.393 ± 0.021
0.669TrpAsn: 0.669 ± 0.027
0.272TrpPro: 0.272 ± 0.017
0.496TrpGln: 0.496 ± 0.021
0.835TrpArg: 0.835 ± 0.031
0.798TrpSer: 0.798 ± 0.034
0.732TrpThr: 0.732 ± 0.029
0.746TrpVal: 0.746 ± 0.03
0.223TrpTrp: 0.223 ± 0.016
0.519TrpTyr: 0.519 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.458TyrAla: 3.458 ± 0.064
0.541TyrCys: 0.541 ± 0.023
2.806TyrAsp: 2.806 ± 0.057
2.371TyrGlu: 2.371 ± 0.054
1.786TyrPhe: 1.786 ± 0.043
3.139TyrGly: 3.139 ± 0.062
0.712TyrHis: 0.712 ± 0.026
2.254TyrIle: 2.254 ± 0.044
1.982TyrLys: 1.982 ± 0.047
3.565TyrLeu: 3.565 ± 0.064
0.965TyrMet: 0.965 ± 0.03
2.008TyrAsn: 2.008 ± 0.063
1.701TyrPro: 1.701 ± 0.04
1.018TyrGln: 1.018 ± 0.032
2.698TyrArg: 2.698 ± 0.053
2.298TyrSer: 2.298 ± 0.049
2.625TyrThr: 2.625 ± 0.057
2.501TyrVal: 2.501 ± 0.051
0.511TyrTrp: 0.511 ± 0.025
1.916TyrTyr: 1.916 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.002
Statistics based on 3142 proteins (1073081 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski