Coriobacteriaceae bacterium
Average proteome isoelectric point is 5.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1631 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V3RRH5|A0A4V3RRH5_9ACTN ArsR family transcriptional regulator OS=Coriobacteriaceae bacterium OX=2011094 GN=E5334_03000 PE=4 SV=1
MM1 pKa = 7.75 CLRR4 pKa = 11.84 GGLMISPEE12 pKa = 4.43 LDD14 pKa = 3.16 EE15 pKa = 5.64 LSCNLIGEE23 pKa = 4.51 ALDD26 pKa = 3.48 VLAAGDD32 pKa = 3.95 LLGVVASVLDD42 pKa = 4.02 GQGNRR47 pKa = 11.84 VTCAFEE53 pKa = 5.91 DD54 pKa = 4.16 DD55 pKa = 4.73 GEE57 pKa = 4.5 DD58 pKa = 3.44 VCLEE62 pKa = 4.25 AAHH65 pKa = 6.76 KK66 pKa = 8.9 WVKK69 pKa = 10.74 DD70 pKa = 3.36 LATGARR76 pKa = 11.84 TEE78 pKa = 4.2 PQLGAPMGYY87 pKa = 10.27 AICYY91 pKa = 9.58 SGAVDD96 pKa = 3.73 TGDD99 pKa = 3.68 GFADD103 pKa = 3.34 ALMCEE108 pKa = 5.09 FGDD111 pKa = 3.65 KK112 pKa = 10.55 DD113 pKa = 3.6 PRR115 pKa = 11.84 PDD117 pKa = 3.2 YY118 pKa = 11.2 SAFLLVQGTGEE129 pKa = 4.23 SFSWSDD135 pKa = 3.36 PAPAGEE141 pKa = 4.71 LPSLLL146 pKa = 4.16
Molecular weight: 15.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.859
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A4V3RQY0|A0A4V3RQY0_9ACTN Antigen 84 OS=Coriobacteriaceae bacterium OX=2011094 GN=E5334_08425 PE=3 SV=1
MM1 pKa = 7.75 ALPGLCLRR9 pKa = 11.84 ASASAAMARR18 pKa = 11.84 HH19 pKa = 5.75 SSAGGGGGGFSARR32 pKa = 11.84 AKK34 pKa = 10.54 GPTGTMPHH42 pKa = 6.35 EE43 pKa = 4.58 FRR45 pKa = 11.84 SKK47 pKa = 10.51 GAGEE51 pKa = 3.96 HH52 pKa = 6.62 RR53 pKa = 11.84 GDD55 pKa = 3.42 NHH57 pKa = 6.99 RR58 pKa = 11.84 SLAAHH63 pKa = 6.95 DD64 pKa = 4.11 GSLRR68 pKa = 11.84 GGVLPAWAGCGIWGSGISGSVRR90 pKa = 11.84 PGSAGTGSHH99 pKa = 7.08 RR100 pKa = 11.84 SPSAGASCCVIADD113 pKa = 3.72 PMRR116 pKa = 11.84 LLRR119 pKa = 11.84 VVGTAGVVPGRR130 pKa = 11.84 KK131 pKa = 8.81 AASGDD136 pKa = 3.8 SDD138 pKa = 4.7 RR139 pKa = 11.84 LPADD143 pKa = 3.95 LGAFRR148 pKa = 11.84 SQCAGRR154 pKa = 11.84 GGLGLEE160 pKa = 4.04 SGGLFRR166 pKa = 11.84 SLAYY170 pKa = 9.82 RR171 pKa = 11.84 RR172 pKa = 11.84 RR173 pKa = 11.84 TCLFQRR179 pKa = 11.84 RR180 pKa = 11.84 SPCPRR185 pKa = 11.84 AGSVLGARR193 pKa = 11.84 IAGFRR198 pKa = 11.84 TLVLGCCNSFSGRR211 pKa = 11.84 KK212 pKa = 8.0 PCRR215 pKa = 11.84 TLHH218 pKa = 6.27 KK219 pKa = 10.38 GLGGAMYY226 pKa = 9.48 EE227 pKa = 4.19 VHH229 pKa = 7.34 DD230 pKa = 4.79 LVLGFARR237 pKa = 11.84 KK238 pKa = 9.24 RR239 pKa = 11.84 LLEE242 pKa = 4.32 HH243 pKa = 7.21 ASLTLEE249 pKa = 4.4 PGRR252 pKa = 11.84 VTGLIAPNGFGKK264 pKa = 7.58 TTLLRR269 pKa = 11.84 WLAGNRR275 pKa = 11.84 SLGQASRR282 pKa = 11.84 RR283 pKa = 11.84 MLDD286 pKa = 3.1 GHH288 pKa = 6.69 KK289 pKa = 10.07 EE290 pKa = 4.09 PKK292 pKa = 9.04 PQAIRR297 pKa = 11.84 SKK299 pKa = 9.85 VFYY302 pKa = 10.12 MPGDD306 pKa = 3.76 GSLLNPQLSGRR317 pKa = 11.84 DD318 pKa = 3.73 HH319 pKa = 6.82 LQTAAQLWKK328 pKa = 10.48 SSADD332 pKa = 2.96 IAQVVTQCSMEE343 pKa = 4.37 PYY345 pKa = 10.01 IEE347 pKa = 3.92 WAVRR351 pKa = 11.84 RR352 pKa = 11.84 LSQGMAQQLALACAYY367 pKa = 9.03 VAQPKK372 pKa = 10.19 YY373 pKa = 10.96 LLLDD377 pKa = 3.75 EE378 pKa = 5.26 PMNALDD384 pKa = 3.86 PTHH387 pKa = 7.01 AEE389 pKa = 4.36 SNGHH393 pKa = 5.57 YY394 pKa = 10.24 LRR396 pKa = 11.84 ALARR400 pKa = 11.84 RR401 pKa = 11.84 GSAVLFSSHH410 pKa = 7.06 ILDD413 pKa = 3.93 NVTAVCDD420 pKa = 3.69 SVLTMEE426 pKa = 4.7 GRR428 pKa = 11.84 RR429 pKa = 11.84 LTEE432 pKa = 3.84 HH433 pKa = 6.56 EE434 pKa = 4.18 NTRR437 pKa = 11.84 SRR439 pKa = 11.84 GFAAEE444 pKa = 4.73 LYY446 pKa = 9.55 RR447 pKa = 11.84 RR448 pKa = 11.84 TYY450 pKa = 8.91 QTVNDD455 pKa = 4.39 APGSAASRR463 pKa = 11.84 QPPRR467 pKa = 3.82
Molecular weight: 49.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 9.502
IPC_protein 10.35
Toseland 10.438
ProMoST 10.292
Dawson 10.584
Bjellqvist 10.35
Wikipedia 10.804
Rodwell 10.672
Grimsley 10.643
Solomon 10.687
Lehninger 10.643
Nozaki 10.496
DTASelect 10.321
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.526
Patrickios 10.306
IPC_peptide 10.687
IPC2_peptide 9.721
IPC2.peptide.svr19 8.521
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1631
0
1631
565989
29
2350
347.0
37.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.497 ± 0.081
1.521 ± 0.029
6.197 ± 0.059
6.266 ± 0.061
3.389 ± 0.037
8.019 ± 0.057
1.963 ± 0.027
4.883 ± 0.046
3.773 ± 0.043
9.542 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.459 ± 0.027
2.915 ± 0.047
4.889 ± 0.051
3.789 ± 0.038
5.412 ± 0.062
6.186 ± 0.046
5.504 ± 0.057
7.85 ± 0.053
1.159 ± 0.025
2.79 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here