SAR202 cluster bacterium AD-802-L14_MRT_200m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YDR9|A0A5N8YDR9_9CHLR FAD-binding protein OS=SAR202 cluster bacterium AD-802-L14_MRT_200m OX=2587837 GN=FIM03_01025 PE=4 SV=1
MM1 pKa = 7.7LYY3 pKa = 10.2FRR5 pKa = 11.84SCMRR9 pKa = 11.84CKK11 pKa = 10.45EE12 pKa = 4.14GTVEE16 pKa = 4.65LGSDD20 pKa = 3.29NFGSFLSCITCGYY33 pKa = 7.97TINSRR38 pKa = 11.84SIRR41 pKa = 11.84SNGSIAEE48 pKa = 4.09PATVDD53 pKa = 3.66EE54 pKa = 4.83EE55 pKa = 4.86VVIAPMLEE63 pKa = 4.05PVLVAEE69 pKa = 4.77DD70 pKa = 3.8VVDD73 pKa = 4.11VEE75 pKa = 4.94TEE77 pKa = 4.08SEE79 pKa = 3.98PDD81 pKa = 2.83IDD83 pKa = 5.93LIAEE87 pKa = 4.08EE88 pKa = 4.37VGAFDD93 pKa = 4.47FEE95 pKa = 4.6EE96 pKa = 4.43VTAII100 pKa = 4.37

Molecular weight: 10.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YEE9|A0A5N8YEE9_9CHLR Uncharacterized protein (Fragment) OS=SAR202 cluster bacterium AD-802-L14_MRT_200m OX=2587837 GN=FIM03_02075 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.48QPKK9 pKa = 8.52SRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84QRR15 pKa = 11.84VHH17 pKa = 6.17GFRR20 pKa = 11.84SRR22 pKa = 11.84MSSKK26 pKa = 10.43GGRR29 pKa = 11.84AIIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.62GRR40 pKa = 11.84IRR42 pKa = 11.84LAVV45 pKa = 3.28

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

819

0

819

240872

34

1253

294.1

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.743 ± 0.075

0.781 ± 0.024

6.149 ± 0.066

6.49 ± 0.092

3.779 ± 0.057

8.384 ± 0.09

2.182 ± 0.044

6.716 ± 0.068

4.064 ± 0.054

8.815 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.038

3.88 ± 0.045

4.61 ± 0.042

3.175 ± 0.048

5.575 ± 0.059

6.776 ± 0.064

5.688 ± 0.046

7.473 ± 0.059

1.475 ± 0.037

2.561 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski