Coccomyxa subellipsoidea (strain C-169) (Green microalga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Trebouxiophyceae incertae sedis; Elliptochloris clade; Coccomyxa; Coccomyxa subellipsoidea

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0YSZ9|I0YSZ9_COCSC Exostosin domain-containing protein OS=Coccomyxa subellipsoidea (strain C-169) OX=574566 GN=COCSUDRAFT_66927 PE=3 SV=1
MM1 pKa = 7.68LEE3 pKa = 4.05PRR5 pKa = 11.84VLHH8 pKa = 7.17DD9 pKa = 4.05IVACTAVVQSPDD21 pKa = 3.02VLTRR25 pKa = 11.84WWLQNANTSRR35 pKa = 11.84APDD38 pKa = 3.52AGIAIPISHH47 pKa = 7.29FSSRR51 pKa = 11.84WHH53 pKa = 6.83LGDD56 pKa = 4.58GPAANDD62 pKa = 4.41GAWPAQPPSLSFAANSDD79 pKa = 3.38DD80 pKa = 3.68GGRR83 pKa = 11.84GGSGAAAPPADD94 pKa = 3.63QTLAATALASIRR106 pKa = 11.84KK107 pKa = 8.43GVTRR111 pKa = 11.84VPVTAVTPPRR121 pKa = 11.84PTSATLASTDD131 pKa = 3.2AQAAIVQQQAAPDD144 pKa = 3.98GEE146 pKa = 4.98AYY148 pKa = 10.23ASRR151 pKa = 11.84VARR154 pKa = 11.84SAARR158 pKa = 11.84VAEE161 pKa = 4.12LAGAAGDD168 pKa = 4.04STAGLMWSSALGSAFQDD185 pKa = 3.03GGAAVVNGASGAVGLADD202 pKa = 4.83DD203 pKa = 4.79AVTGAGVQAAQDD215 pKa = 3.68MSTLRR220 pKa = 11.84DD221 pKa = 3.39GLAAAVTPIANATVATAAAARR242 pKa = 11.84QALTQGIEE250 pKa = 4.01DD251 pKa = 3.91TTEE254 pKa = 3.42QFAPVLAPLRR264 pKa = 11.84PGSASEE270 pKa = 4.06ALAPAVAQIGDD281 pKa = 3.33WWRR284 pKa = 11.84QSQEE288 pKa = 3.69RR289 pKa = 11.84ARR291 pKa = 11.84SSLNDD296 pKa = 3.44FLTPALAGVANVVSPQKK313 pKa = 10.53RR314 pKa = 11.84EE315 pKa = 3.34EE316 pKa = 4.13CMQKK320 pKa = 8.79FTHH323 pKa = 6.93CGFCSPNFDD332 pKa = 3.17RR333 pKa = 11.84CLFCTAPFTLIDD345 pKa = 4.34ISCACPPGTFVSSDD359 pKa = 3.25GSSCIPFAVAQLEE372 pKa = 4.1KK373 pKa = 10.7LILAISQQNAALADD387 pKa = 3.93RR388 pKa = 11.84VNSLLHH394 pKa = 6.18LVGPSRR400 pKa = 11.84TCQANIPFCLRR411 pKa = 11.84CSSPATCAVCSDD423 pKa = 4.28GYY425 pKa = 11.47KK426 pKa = 10.23LDD428 pKa = 4.26AVGKK432 pKa = 7.93CICDD436 pKa = 3.5PTTAGPGCAFCIVPGSCGNPLGGTKK461 pKa = 10.58ANLKK465 pKa = 10.07ACADD469 pKa = 3.75FGKK472 pKa = 10.1RR473 pKa = 11.84RR474 pKa = 11.84QYY476 pKa = 9.96GRR478 pKa = 11.84AYY480 pKa = 10.81NFVKK484 pKa = 10.8DD485 pKa = 3.72PVTGLGRR492 pKa = 11.84CEE494 pKa = 4.99CNPQSTPHH502 pKa = 6.49CAACLFPGVCKK513 pKa = 10.59FCNAPFEE520 pKa = 4.52LVDD523 pKa = 4.62GKK525 pKa = 10.05CACPPLYY532 pKa = 10.25GPPVMDD538 pKa = 5.34EE539 pKa = 4.22DD540 pKa = 4.0TCLPIFLGQQVYY552 pKa = 7.79ITGPTGPTGFGPTGPTGIAIGATGFTGSTAYY583 pKa = 10.32GVTGFTGFTGFTGFTGFTGPTGFTGFTGDD612 pKa = 3.06TGFTGFTGDD621 pKa = 3.06TGFTGFTGDD630 pKa = 3.06TGFTGFTGDD639 pKa = 3.22TGFTGTTGDD648 pKa = 3.25TGFTGFTGDD657 pKa = 3.46TGFTGNTGDD666 pKa = 3.72TGFTGATGFTGDD678 pKa = 3.64TGFTGDD684 pKa = 3.77TGFTGNTGDD693 pKa = 3.72TGFTGATGFTGDD705 pKa = 3.43TGTTGFTGTTGNTGDD720 pKa = 3.7TGFTGDD726 pKa = 3.32TGFTGFTGATGDD738 pKa = 3.57TGFTGNTGDD747 pKa = 3.39TGFTGFTGNTGFTGFTGDD765 pKa = 3.31TGFTGDD771 pKa = 3.72TGFTGATGFTGDD783 pKa = 3.31TGFTGFTGSTGFTGFTGDD801 pKa = 3.06TGFTGFTGFTGATGNTGDD819 pKa = 3.62TGFTGFTGDD828 pKa = 3.06TGFTGFTGDD837 pKa = 3.06TGFTGFTGFTGDD849 pKa = 3.46TGFTGNTGDD858 pKa = 3.72TGFTGATGFSGSTGFTGDD876 pKa = 3.18TGFAGATGFTGFTGDD891 pKa = 3.42TGFTGATGVTGFTGATGYY909 pKa = 10.91SGDD912 pKa = 3.83TGFTGATGFSGFTGFTGGTGFTGFTGDD939 pKa = 3.03TGFTGFPGFTGATGFTGFTGATGFTGDD966 pKa = 3.31TGFTGFTGNTGFTGATGFTGFTGFTGDD993 pKa = 3.42TGFTGATGFTGFTGATGFTGSTGFTGDD1020 pKa = 2.93TGFTGVSGFTGDD1032 pKa = 3.32TGFTGATGFTGFTGATGFTGDD1053 pKa = 4.0TGISGATGFTGFTGFTGATGFTGTTGFTGATGLTGDD1089 pKa = 4.18SGFTGTTGFTGFTGDD1104 pKa = 3.06TGFTGFTGFTGFTGATGFTGDD1125 pKa = 4.09TGNTGATGFTGFTGATGFTGDD1146 pKa = 3.31TGFTGFTGATGFTGATGFTGDD1167 pKa = 3.31TGFTGFTGFTGFTGDD1182 pKa = 3.42TGFTGATGFTGFTGATGITGDD1203 pKa = 3.89TGITGATGFTGATGYY1218 pKa = 9.12TGFTGNTGSTGSTGATGFTGATGYY1242 pKa = 9.24TGTTGFTGFTGDD1254 pKa = 3.46TGFTGNTGDD1263 pKa = 3.43TGFTGVTGFTGATGFTGSTGDD1284 pKa = 3.36TGFTGATGFTGFTGATGFTGSTGFTGTTGFTGATGFTGFTGFTGTTGATGFTGFTGATGFTGFTGFTGATGFTGATGSTGFTGFTGDD1371 pKa = 3.5TGITGATGFTGDD1383 pKa = 3.55TGFTGTTGFTGFTGDD1398 pKa = 3.42TGFTGATGFTGTTGFTGATGFTGFTGDD1425 pKa = 3.21TGITGGTGFTGSTGFTGNTGSTGATGITGFTGATGFTGDD1464 pKa = 3.76TGFTGATGFTGFTGDD1479 pKa = 3.42TGFTGATGSTGFTGATGFTGFTGATGFTGFTGGTGYY1515 pKa = 9.2TGTTGFTGATGFTGFTGDD1533 pKa = 3.5TGITGATGFTGSTGFTGTTGFTGFTGDD1560 pKa = 3.23TGFTGSTGATGFTGATGFTGGTGFTGFTGSTGFTGAIGFTGATGFTGFTGDD1611 pKa = 3.23TGFTGSTGFTGFTGDD1626 pKa = 3.42TGFTGATGFTGATGNTGFTGATGFTGTTGFTGFTGDD1662 pKa = 3.42TGFTGATGSTGFTGSTGFSGATGFSGATGFTGFTGATGFTGDD1704 pKa = 3.39TGATGFSGATGFTGSTGGTGATGYY1728 pKa = 10.76SGFTGATGFTGDD1740 pKa = 3.82SGFTGTTGATGYY1752 pKa = 8.98TGSTGFTGATGFTGFTGATGYY1773 pKa = 9.01TGATGFTGFTGDD1785 pKa = 3.06TGFTGFTGNTGFTGATGFTGSTGATGFTGATGLTGFTGATGSTGTSGSTGFTGATGFTGVTGATGFTGSTGFTGSTGFTGSTGFTGATGSTGATGDD1881 pKa = 3.43TGFTGNTGFTGNTGFTGGTGATGYY1905 pKa = 8.99TGATGFTGFTGTTGDD1920 pKa = 3.42TGITGSTGYY1929 pKa = 8.97TGSTGATGFSGATGFTGFTGDD1950 pKa = 3.42TGFTGATGFTGSTGVTGFTGGTGYY1974 pKa = 9.11TGFTGSTGATGFTGVTGFTGDD1995 pKa = 3.06TGATGFSGATGATGVTGSTGFTGSTGFTGATGFTGDD2031 pKa = 3.7TGATGLTGASGFTGNTGFTGATGFTGFTGATGFTGFTGFTGATGFTGNTGATGASGFTGSTGGTGFTGATGFTGSTGFTGFTGDD2115 pKa = 3.64TGFSGATGFTGSTGGTGTTGATGFTGSTGGTGSSGATGATGGSGATGFTGGTGATGLTGNTGATGSTGGTGFTGTTGGTGATGDD2199 pKa = 3.63TGSTGGTGITGVTGGTGALGSTGATGFTGDD2229 pKa = 3.81TGITGATGFSGATGATGFSGPSGVTGITGATGFTGSSGDD2268 pKa = 3.53TGVTGATGSRR2278 pKa = 11.84GFIGATGFTGAIGTTGSTGFTGGTGATGGTGGTGFTGATGSTGTTGGTGGTGGTGASGSTGNTGASGFTGSTGGTGATGFTGQTGGTGSTGTTGATGSTGTTGSSGITGSTGSTGSSGLQGFTGATGATGNSGTTGATGLTGASGATGFTGSTGGSGATGSTGFSGATGTTGATGFSGGSGATGSTGFSGATGGTGSTGGTGSTGATGGTGFSGATGFTGTTGGTGFTGFSGATGGSGFTGATGAAATGATGVTGNTGPTGGTGPSGSTGAVGTSGATGSTGVTGTTGTSGPTGFSGGTGSTGPGITGATGVTGPTGPTGPTGFTGGTGGTGGTGFAATGATGPTGPTGAQGVTGGAGG2636 pKa = 2.87

Molecular weight: 244.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0Z022|I0Z022_COCSC DnaJ-domain-containing protein OS=Coccomyxa subellipsoidea (strain C-169) OX=574566 GN=COCSUDRAFT_53179 PE=4 SV=1
MM1 pKa = 7.59SLPLAPPATRR11 pKa = 11.84PKK13 pKa = 9.98HH14 pKa = 5.68SKK16 pKa = 10.24RR17 pKa = 11.84SPLSAQTRR25 pKa = 11.84QQRR28 pKa = 11.84PRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84ASAVMMSALTQALRR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84TAGRR57 pKa = 11.84AA58 pKa = 3.13

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9785

14

9799

4166057

31

15797

425.2

46.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.249 ± 0.063

1.525 ± 0.012

5.111 ± 0.019

6.122 ± 0.027

3.241 ± 0.02

7.899 ± 0.042

2.278 ± 0.011

3.633 ± 0.022

4.17 ± 0.023

9.739 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.01

2.77 ± 0.018

5.941 ± 0.031

4.575 ± 0.021

6.253 ± 0.026

7.187 ± 0.024

4.961 ± 0.041

6.525 ± 0.022

1.356 ± 0.011

2.226 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski