Shewanella mangrovi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094J9Y0|A0A094J9Y0_9GAMM Serine protease OS=Shewanella mangrovi OX=1515746 GN=HR45_18480 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.35INLALQLSAVLVSASAFSADD22 pKa = 3.52MVITGVVDD30 pKa = 4.6GSLSGGIPKK39 pKa = 10.18AVEE42 pKa = 4.09LYY44 pKa = 9.98VVNDD48 pKa = 3.1ISDD51 pKa = 4.19LSRR54 pKa = 11.84CGIDD58 pKa = 3.31VASNGATTAGNHH70 pKa = 4.8STFSGSATAGSFLYY84 pKa = 10.58AATEE88 pKa = 4.45TPGFTAFFGFEE99 pKa = 3.91PTLRR103 pKa = 11.84TPSFNGNDD111 pKa = 3.59VVLLQCDD118 pKa = 3.85GVVIDD123 pKa = 4.99SFGEE127 pKa = 3.98LGVDD131 pKa = 3.75GLGTPWEE138 pKa = 4.41YY139 pKa = 10.35TDD141 pKa = 3.75SWAHH145 pKa = 6.04RR146 pKa = 11.84NSGTTADD153 pKa = 3.8GNSFNLTNWSFSGPNTLDD171 pKa = 3.35NQTSNATATIPYY183 pKa = 8.76PLASFVYY190 pKa = 8.62GTDD193 pKa = 3.59DD194 pKa = 3.76GSTGGDD200 pKa = 3.1TGTGGDD206 pKa = 3.89TGTGGG211 pKa = 3.43

Molecular weight: 21.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094JAE8|A0A094JAE8_9GAMM Nuclease OS=Shewanella mangrovi OX=1515746 GN=HR45_13650 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3384

0

3384

1119320

36

2024

330.8

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.986 ± 0.043

1.057 ± 0.016

5.582 ± 0.035

5.76 ± 0.042

3.863 ± 0.027

6.968 ± 0.041

2.266 ± 0.021

5.717 ± 0.032

4.661 ± 0.034

10.8 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.023

3.933 ± 0.032

4.017 ± 0.021

5.181 ± 0.039

4.843 ± 0.036

6.323 ± 0.032

5.157 ± 0.031

6.966 ± 0.032

1.26 ± 0.019

3.002 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski