Torque teno midi virus 11
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLZ2|A7VLZ2_9VIRU Capsid protein OS=Torque teno midi virus 11 OX=2065052 PE=3 SV=1
MM1 pKa = 7.43 SNYY4 pKa = 9.79 SANHH8 pKa = 5.58 FFKK11 pKa = 9.81 PTPYY15 pKa = 11.19 NNDD18 pKa = 3.14 TKK20 pKa = 10.99 NQMWMSMISDD30 pKa = 3.76 GHH32 pKa = 7.87 DD33 pKa = 3.38 CMCNCPSPFGHH44 pKa = 7.32 LLASIFPPGHH54 pKa = 5.66 QDD56 pKa = 2.75 RR57 pKa = 11.84 FLTVEE62 pKa = 4.18 EE63 pKa = 4.78 IINRR67 pKa = 11.84 DD68 pKa = 2.84 IKK70 pKa = 10.74 EE71 pKa = 4.23 CLFGGPDD78 pKa = 3.32 ARR80 pKa = 11.84 DD81 pKa = 3.54 GGEE84 pKa = 3.83 AAAGPSTGVDD94 pKa = 3.27 TTIKK98 pKa = 10.65 RR99 pKa = 11.84 EE100 pKa = 4.03 DD101 pKa = 3.66 PEE103 pKa = 4.24 EE104 pKa = 4.81 DD105 pKa = 3.17 FTKK108 pKa = 10.91 EE109 pKa = 3.95 NIEE112 pKa = 4.02 EE113 pKa = 4.6 LIAAAEE119 pKa = 4.24 KK120 pKa = 10.61 ASEE123 pKa = 4.08 RR124 pKa = 3.99
Molecular weight: 13.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.577
IPC2_protein 4.596
IPC_protein 4.507
Toseland 4.355
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.329
Rodwell 4.355
Grimsley 4.279
Solomon 4.457
Lehninger 4.406
Nozaki 4.571
DTASelect 4.724
Thurlkill 4.368
EMBOSS 4.355
Sillero 4.622
Patrickios 3.745
IPC_peptide 4.457
IPC2_peptide 4.622
IPC2.peptide.svr19 4.538
Protein with the highest isoelectric point:
>tr|A7VLZ2|A7VLZ2_9VIRU Capsid protein OS=Torque teno midi virus 11 OX=2065052 PE=3 SV=1
MM1 pKa = 7.31 PFWWARR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.46 RR10 pKa = 11.84 WWRR13 pKa = 11.84 GRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 PFYY20 pKa = 10.23 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 YY24 pKa = 7.77 HH25 pKa = 5.21 YY26 pKa = 10.11 KK27 pKa = 9.06 KK28 pKa = 9.73 RR29 pKa = 11.84 RR30 pKa = 11.84 PRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 LYY36 pKa = 10.04 KK37 pKa = 9.27 RR38 pKa = 11.84 KK39 pKa = 9.35 HH40 pKa = 5.07 RR41 pKa = 11.84 RR42 pKa = 11.84 TYY44 pKa = 9.27 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 9.42 SFRR52 pKa = 11.84 KK53 pKa = 8.97 VRR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 10.05 RR58 pKa = 11.84 RR59 pKa = 11.84 TLPLKK64 pKa = 9.27 QWQPEE69 pKa = 4.35 TIRR72 pKa = 11.84 KK73 pKa = 9.43 CKK75 pKa = 10.14 VKK77 pKa = 10.79 GIGIHH82 pKa = 4.78 TLGVEE87 pKa = 4.66 GTQYY91 pKa = 11.03 RR92 pKa = 11.84 CYY94 pKa = 9.66 TANKK98 pKa = 9.43 YY99 pKa = 10.36 EE100 pKa = 4.0 YY101 pKa = 9.48 TIAKK105 pKa = 8.75 QPSGGGFGVEE115 pKa = 4.2 RR116 pKa = 11.84 YY117 pKa = 7.44 TLEE120 pKa = 3.97 YY121 pKa = 10.32 LYY123 pKa = 10.79 KK124 pKa = 10.04 EE125 pKa = 4.3 HH126 pKa = 7.66 KK127 pKa = 10.28 AGNNVWTTSNVNLDD141 pKa = 3.41 LVRR144 pKa = 11.84 YY145 pKa = 6.23 TGCRR149 pKa = 11.84 FIFWRR154 pKa = 11.84 HH155 pKa = 3.98 PHH157 pKa = 6.03 IDD159 pKa = 3.41 FVGVYY164 pKa = 9.66 SRR166 pKa = 11.84 NYY168 pKa = 9.26 PMQLQKK174 pKa = 10.04 YY175 pKa = 6.44 TYY177 pKa = 9.49 PDD179 pKa = 3.22 THH181 pKa = 6.77 PQQIMLAKK189 pKa = 9.53 HH190 pKa = 5.82 KK191 pKa = 10.46 FFIPSLLTKK200 pKa = 9.7 PHH202 pKa = 5.8 GKK204 pKa = 9.77 RR205 pKa = 11.84 YY206 pKa = 10.29 VKK208 pKa = 10.36 IKK210 pKa = 10.27 IKK212 pKa = 10.6 PPTQMSNKK220 pKa = 8.79 WFFQEE225 pKa = 4.48 GFSDD229 pKa = 3.44 TGLVQIHH236 pKa = 5.95 TAACDD241 pKa = 3.23 LRR243 pKa = 11.84 YY244 pKa = 10.19 AHH246 pKa = 7.32 LGCCNTNQLASFLTLNTDD264 pKa = 4.78 FYY266 pKa = 11.62 QYY268 pKa = 11.27 AAWGNPKK275 pKa = 10.07 NPYY278 pKa = 9.92 IPTEE282 pKa = 3.55 QDD284 pKa = 2.06 KK285 pKa = 10.0 WYY287 pKa = 9.4 RR288 pKa = 11.84 PYY290 pKa = 10.81 RR291 pKa = 11.84 IVEE294 pKa = 4.09 PVTEE298 pKa = 4.16 VEE300 pKa = 4.31 DD301 pKa = 3.55 VKK303 pKa = 11.4 GNKK306 pKa = 8.17 KK307 pKa = 9.15 TITTVFTPYY316 pKa = 10.51 TEE318 pKa = 4.46 TITYY322 pKa = 9.28 NKK324 pKa = 10.24 GWFQPALLQAKK335 pKa = 9.64 QITKK339 pKa = 9.82 PSQANPPLKK348 pKa = 9.76 GARR351 pKa = 11.84 YY352 pKa = 9.94 NPTLDD357 pKa = 3.27 TGEE360 pKa = 4.2 NTAVWLTNVVNQSYY374 pKa = 9.61 YY375 pKa = 10.04 PPKK378 pKa = 9.68 TDD380 pKa = 4.76 LDD382 pKa = 4.28 LFVQGLPLWQLLLGFPDD399 pKa = 3.51 WVRR402 pKa = 11.84 QKK404 pKa = 11.16 KK405 pKa = 9.75 KK406 pKa = 10.87 DD407 pKa = 3.46 PTFLQSYY414 pKa = 6.91 YY415 pKa = 11.29 LCFHH419 pKa = 7.43 CDD421 pKa = 3.49 AIEE424 pKa = 4.28 PKK426 pKa = 10.19 PGLHH430 pKa = 5.58 QVFIPVDD437 pKa = 3.4 YY438 pKa = 11.31 SFVQGKK444 pKa = 9.68 GPYY447 pKa = 9.92 DD448 pKa = 3.56 SLLSKK453 pKa = 9.67 WDD455 pKa = 3.43 NDD457 pKa = 2.88 HH458 pKa = 7.25 WYY460 pKa = 7.43 PTLKK464 pKa = 10.55 HH465 pKa = 5.72 QLQTINSVVEE475 pKa = 3.95 TGPFVPKK482 pKa = 10.55 LDD484 pKa = 3.73 TLKK487 pKa = 10.99 NSTWEE492 pKa = 4.17 LYY494 pKa = 8.96 STYY497 pKa = 10.52 TFYY500 pKa = 11.19 FKK502 pKa = 10.85 FGGASLPEE510 pKa = 5.08 AEE512 pKa = 4.86 TKK514 pKa = 10.87 DD515 pKa = 3.49 PATQGTHH522 pKa = 5.54 EE523 pKa = 4.55 VPDD526 pKa = 4.0 KK527 pKa = 10.38 QSKK530 pKa = 10.09 AIQIINPKK538 pKa = 10.05 KK539 pKa = 8.47 NTAAAALHH547 pKa = 6.22 CWDD550 pKa = 4.23 FRR552 pKa = 11.84 RR553 pKa = 11.84 GHH555 pKa = 4.94 ITSRR559 pKa = 11.84 AFKK562 pKa = 10.37 RR563 pKa = 11.84 MCEE566 pKa = 3.88 NAEE569 pKa = 3.98 TDD571 pKa = 3.16 SDD573 pKa = 3.98 FQADD577 pKa = 3.72 VSPKK581 pKa = 8.75 KK582 pKa = 9.3 TKK584 pKa = 10.01 TSLQEE589 pKa = 3.68 NTLPCISKK597 pKa = 10.05 EE598 pKa = 3.97 AEE600 pKa = 3.96 EE601 pKa = 4.39 VQDD604 pKa = 4.47 CLQSLYY610 pKa = 11.03 EE611 pKa = 4.15 EE612 pKa = 4.37 NTFQEE617 pKa = 5.35 IPDD620 pKa = 3.66 QQNIQQLLHH629 pKa = 5.65 QQQQQQQHH637 pKa = 5.24 TKK639 pKa = 10.42 LQLLQLINNLKK650 pKa = 10.22 KK651 pKa = 10.18 KK652 pKa = 10.37 QKK654 pKa = 10.39 LIQLQTGILDD664 pKa = 3.51
Molecular weight: 78.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.936
IPC2_protein 9.268
IPC_protein 9.194
Toseland 9.823
ProMoST 9.575
Dawson 10.072
Bjellqvist 9.78
Wikipedia 10.248
Rodwell 10.438
Grimsley 10.16
Solomon 10.087
Lehninger 10.043
Nozaki 9.867
DTASelect 9.75
Thurlkill 9.911
EMBOSS 10.233
Sillero 9.999
Patrickios 5.639
IPC_peptide 10.087
IPC2_peptide 8.463
IPC2.peptide.svr19 8.288
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1321
124
664
330.3
38.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.98 ± 1.204
2.044 ± 0.214
4.466 ± 0.511
6.056 ± 1.244
4.391 ± 0.406
4.769 ± 0.368
3.482 ± 0.355
4.542 ± 0.276
8.554 ± 0.945
6.889 ± 1.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.741 ± 0.679
4.921 ± 0.443
7.419 ± 1.184
5.829 ± 1.283
5.829 ± 0.696
6.435 ± 2.421
7.873 ± 0.423
3.104 ± 0.928
1.665 ± 0.698
4.012 ± 1.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here