Strawberry pallidosis-associated virus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6JGW2|Q6JGW2_9CLOS Movement protein OS=Strawberry pallidosis-associated virus OX=227507 PE=4 SV=1
MM1 pKa = 7.59 LFFVLIVSDD10 pKa = 3.4 IGFNSYY16 pKa = 10.64 YY17 pKa = 10.83 LSDD20 pKa = 3.4 NKK22 pKa = 10.44 EE23 pKa = 4.12 FEE25 pKa = 4.64 GEE27 pKa = 4.34 LIPIVTEE34 pKa = 3.87 DD35 pKa = 3.42 TTILIDD41 pKa = 3.99 LLRR44 pKa = 11.84 ICSFIKK50 pKa = 10.36 EE51 pKa = 4.02 RR52 pKa = 11.84 WW53 pKa = 3.29
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.324
IPC2_protein 4.279
IPC_protein 4.075
Toseland 3.91
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.923
Grimsley 3.834
Solomon 4.024
Lehninger 3.986
Nozaki 4.177
DTASelect 4.317
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.19
Patrickios 3.592
IPC_peptide 4.024
IPC2_peptide 4.177
IPC2.peptide.svr19 4.103
Protein with the highest isoelectric point:
>tr|Q6JGW3|Q6JGW3_9CLOS ORF 2 OS=Strawberry pallidosis-associated virus OX=227507 PE=4 SV=1
MM1 pKa = 7.85 DD2 pKa = 5.24 KK3 pKa = 10.9 EE4 pKa = 4.37 SALHH8 pKa = 5.59 VLLVLWHH15 pKa = 6.16 IVWHH19 pKa = 5.7 AMCYY23 pKa = 9.54 IYY25 pKa = 10.88 VVSFTARR32 pKa = 11.84 DD33 pKa = 3.18 HH34 pKa = 6.54 RR35 pKa = 11.84 RR36 pKa = 11.84 FVRR39 pKa = 11.84 PPHH42 pKa = 5.66 TPQFKK47 pKa = 10.23 RR48 pKa = 11.84 KK49 pKa = 8.8 RR50 pKa = 11.84 KK51 pKa = 9.44 LPRR54 pKa = 11.84 ILLNVLAFF62 pKa = 4.2
Molecular weight: 7.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.984
IPC_protein 10.994
Toseland 11.111
ProMoST 11.125
Dawson 11.155
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.272
Grimsley 11.199
Solomon 11.418
Lehninger 11.359
Nozaki 11.096
DTASelect 10.965
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.111
Patrickios 11.052
IPC_peptide 11.418
IPC2_peptide 10.218
IPC2.peptide.svr19 8.514
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4980
53
2451
498.0
57.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.775 ± 0.453
1.968 ± 0.28
6.426 ± 0.332
5.823 ± 0.4
6.145 ± 0.484
4.639 ± 0.396
1.647 ± 0.28
7.309 ± 0.527
7.972 ± 0.304
9.598 ± 0.568
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.47 ± 0.37
6.606 ± 0.469
3.092 ± 0.166
2.229 ± 0.177
4.819 ± 0.294
8.454 ± 0.27
5.201 ± 0.372
6.747 ± 0.348
0.582 ± 0.081
4.498 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here