Spiroplasma sabaudiense Ar-1343

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma sabaudiense

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6A9M4|W6A9M4_9MOLU Uncharacterized protein OS=Spiroplasma sabaudiense Ar-1343 OX=1276257 GN=SSABA_v1c01780 PE=4 SV=1
MM1 pKa = 7.3EE2 pKa = 5.59RR3 pKa = 11.84KK4 pKa = 9.17FLSSDD9 pKa = 3.38FKK11 pKa = 11.16IFNDD15 pKa = 3.79PKK17 pKa = 11.15NIMKK21 pKa = 9.98EE22 pKa = 4.01LFGIACSLCGDD33 pKa = 3.89EE34 pKa = 4.51EE35 pKa = 4.28IKK37 pKa = 11.0YY38 pKa = 9.54VASNAPQSIGEE49 pKa = 4.2IATEE53 pKa = 3.74ALEE56 pKa = 4.58DD57 pKa = 3.83NPNITDD63 pKa = 3.72SEE65 pKa = 4.48LDD67 pKa = 3.48EE68 pKa = 5.39MIDD71 pKa = 3.68EE72 pKa = 5.85PISIWQQFDD81 pKa = 3.54DD82 pKa = 4.57EE83 pKa = 4.42NAEE86 pKa = 3.96NLIPTFSCEE95 pKa = 3.58VCYY98 pKa = 10.32EE99 pKa = 3.8QLLNGEE105 pKa = 4.19ITLL108 pKa = 4.24

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6ABJ9|W6ABJ9_9MOLU Uncharacterized protein OS=Spiroplasma sabaudiense Ar-1343 OX=1276257 GN=SSABA_v1c07930 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21NGRR28 pKa = 11.84KK29 pKa = 8.75IVKK32 pKa = 9.35ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84ARR41 pKa = 11.84LTAA44 pKa = 4.19

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

924

0

924

322205

37

2305

348.7

39.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.49 ± 0.085

0.615 ± 0.018

5.34 ± 0.058

6.948 ± 0.085

5.834 ± 0.068

5.229 ± 0.072

1.254 ± 0.03

9.775 ± 0.082

9.309 ± 0.08

9.39 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.027

7.292 ± 0.083

2.785 ± 0.035

3.486 ± 0.039

3.002 ± 0.052

6.699 ± 0.061

5.084 ± 0.049

5.793 ± 0.053

1.052 ± 0.028

3.536 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski