Jaapia argillacea MUCL 33604
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16341 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067Q6T3|A0A067Q6T3_9AGAM Uncharacterized protein OS=Jaapia argillacea MUCL 33604 OX=933084 GN=JAAARDRAFT_655672 PE=4 SV=1
MM1 pKa = 6.97 LHH3 pKa = 7.31 KK4 pKa = 9.59 YY5 pKa = 7.82 TNHH8 pKa = 6.37 GDD10 pKa = 3.85 LLSSASHH17 pKa = 6.69 EE18 pKa = 4.14 GMEE21 pKa = 4.32 MDD23 pKa = 3.74 WVPVQSEE30 pKa = 4.64 EE31 pKa = 4.27 PVWLYY36 pKa = 11.16 GSQDD40 pKa = 2.69 IYY42 pKa = 11.53 QNDD45 pKa = 3.82 VQVHH49 pKa = 4.66 MGEE52 pKa = 4.12 HH53 pKa = 6.27 LEE55 pKa = 3.98 NRR57 pKa = 11.84 GVFTQDD63 pKa = 2.53 VFGAPAIQGTSFVDD77 pKa = 2.97 KK78 pKa = 10.25 DD79 pKa = 3.71 EE80 pKa = 5.07 RR81 pKa = 11.84 MSPPPSSPSYY91 pKa = 10.44 HH92 pKa = 6.27 QDD94 pKa = 3.52 APPMSYY100 pKa = 10.65 PSIRR104 pKa = 11.84 PPLTHH109 pKa = 6.39 HH110 pKa = 7.01 TNPTSPSPDD119 pKa = 3.26 CSHH122 pKa = 7.59 RR123 pKa = 11.84 DD124 pKa = 3.72 TNHH127 pKa = 5.71 MALNSFEE134 pKa = 5.22 DD135 pKa = 4.64 LPDD138 pKa = 4.52 GDD140 pKa = 4.2 IAPPSSQDD148 pKa = 2.99 PRR150 pKa = 11.84 IAPRR154 pKa = 11.84 ANYY157 pKa = 9.21 VALDD161 pKa = 3.88 SFADD165 pKa = 4.53 LPDD168 pKa = 5.42 DD169 pKa = 6.48 DD170 pKa = 5.04 IAPPNNDD177 pKa = 2.37 ITPPGSQEE185 pKa = 3.57 MQIIVPCGRR194 pKa = 11.84 HH195 pKa = 4.16 VQSPRR200 pKa = 11.84 QDD202 pKa = 3.35 MQEE205 pKa = 4.53 PLPPMYY211 pKa = 9.86 IDD213 pKa = 5.19 GDD215 pKa = 4.05 DD216 pKa = 5.26 DD217 pKa = 4.49 INPPSDD223 pKa = 4.7 DD224 pKa = 3.94 LPTYY228 pKa = 10.38 IDD230 pKa = 3.98 NDD232 pKa = 3.67 NNIPPPSQEE241 pKa = 4.29 ALVASPTHH249 pKa = 6.4 YY250 pKa = 10.1 IDD252 pKa = 4.79 SDD254 pKa = 4.09 DD255 pKa = 5.73 DD256 pKa = 3.81 IPPPSQEE263 pKa = 4.59 APTFSPPSYY272 pKa = 10.31 INKK275 pKa = 9.35 DD276 pKa = 3.67 DD277 pKa = 5.3 NIPPPSQEE285 pKa = 4.33 ALTFLPPTYY294 pKa = 10.55 INDD297 pKa = 4.62 DD298 pKa = 4.13 DD299 pKa = 6.18 DD300 pKa = 4.49 ISPPSQEE307 pKa = 5.16 AIILSPSIHH316 pKa = 6.7 INNNDD321 pKa = 3.04 ILTSHH326 pKa = 7.19
Molecular weight: 35.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.897
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.062
Patrickios 1.38
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.931
Protein with the highest isoelectric point:
>tr|A0A067QPK5|A0A067QPK5_9AGAM Uncharacterized protein OS=Jaapia argillacea MUCL 33604 OX=933084 GN=JAAARDRAFT_684114 PE=4 SV=1
MM1 pKa = 7.35 CMWLRR6 pKa = 11.84 GSMSMSVSLNFWGIPARR23 pKa = 11.84 RR24 pKa = 11.84 TSRR27 pKa = 11.84 RR28 pKa = 11.84 SLPTPPSAAAATTTMPVPNRR48 pKa = 11.84 LLWRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 LQFYY59 pKa = 10.27 SPSPTSTATSLPTTRR74 pKa = 11.84 PMLLFVTPFVLRR86 pKa = 11.84 TVSILPPTTTTTTTTTILTIPLPASRR112 pKa = 11.84 INPSSMSIPIAASINAPRR130 pKa = 11.84 ITQQ133 pKa = 3.24
Molecular weight: 14.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.373
IPC2_protein 10.95
IPC_protein 12.413
Toseland 12.559
ProMoST 13.071
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.062
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.071
Sillero 12.559
Patrickios 11.798
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16339
2
16341
6436100
22
5100
393.9
43.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.739 ± 0.017
1.387 ± 0.009
5.496 ± 0.014
5.869 ± 0.02
3.784 ± 0.013
6.679 ± 0.018
2.656 ± 0.01
4.92 ± 0.014
4.415 ± 0.02
9.463 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.074 ± 0.007
3.302 ± 0.01
6.844 ± 0.026
3.642 ± 0.012
6.144 ± 0.017
9.001 ± 0.027
6.036 ± 0.013
6.427 ± 0.013
1.516 ± 0.007
2.607 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here