Phaius virus X
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0I2Z6|B0I2Z6_9VIRU Coat protein OS=Phaius virus X OX=457382 PE=3 SV=1
MM1 pKa = 7.21 EE2 pKa = 5.71 AATGMARR9 pKa = 11.84 SPYY12 pKa = 7.99 TTAQQRR18 pKa = 11.84 EE19 pKa = 4.66 GATPTLTSSGLSWLFQGSSLPFIGLGLAALALALALGVSNPRR61 pKa = 11.84 QCLITIDD68 pKa = 3.56 GAGAILQGCGEE79 pKa = 4.3 IQDD82 pKa = 4.18 LPAVINALHH91 pKa = 5.65 YY92 pKa = 10.89 AKK94 pKa = 10.52 GLSYY98 pKa = 10.9 PNIRR102 pKa = 11.84 NCEE105 pKa = 4.01 YY106 pKa = 10.51 GYY108 pKa = 10.57 HH109 pKa = 5.05 EE110 pKa = 4.84 HH111 pKa = 7.1 CEE113 pKa = 3.95 QVRR116 pKa = 11.84 SCEE119 pKa = 4.06 HH120 pKa = 6.71 ASLDD124 pKa = 4.06 GPP126 pKa = 4.19
Molecular weight: 13.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.315
IPC2_protein 5.308
IPC_protein 5.207
Toseland 5.385
ProMoST 5.448
Dawson 5.334
Bjellqvist 5.397
Wikipedia 5.258
Rodwell 5.296
Grimsley 5.41
Solomon 5.334
Lehninger 5.308
Nozaki 5.525
DTASelect 5.664
Thurlkill 5.55
EMBOSS 5.486
Sillero 5.614
Patrickios 0.566
IPC_peptide 5.347
IPC2_peptide 5.626
IPC2.peptide.svr19 5.563
Protein with the highest isoelectric point:
>tr|B0I2Z5|B0I2Z5_9VIRU Movement protein TGBp3 OS=Phaius virus X OX=457382 PE=3 SV=1
MM1 pKa = 7.88 PLQPPTDD8 pKa = 3.51 YY9 pKa = 11.03 TRR11 pKa = 11.84 IYY13 pKa = 9.21 SFLVIGVSCVTILYY27 pKa = 10.27 LLTRR31 pKa = 11.84 STLPHH36 pKa = 6.48 VGDD39 pKa = 4.85 NIHH42 pKa = 6.59 HH43 pKa = 6.84 LPHH46 pKa = 6.61 GGCYY50 pKa = 9.29 RR51 pKa = 11.84 DD52 pKa = 3.79 GTKK55 pKa = 10.28 SIYY58 pKa = 10.12 YY59 pKa = 10.04 GSAKK63 pKa = 10.0 RR64 pKa = 11.84 GSHH67 pKa = 7.25 PYY69 pKa = 9.39 PDD71 pKa = 4.25 KK72 pKa = 11.06 LWAFLAISGLVFAIYY87 pKa = 6.92 WTRR90 pKa = 11.84 PRR92 pKa = 11.84 RR93 pKa = 11.84 ACPCTCPRR101 pKa = 11.84 CVEE104 pKa = 4.27 PSAVPNNNN112 pKa = 2.9
Molecular weight: 12.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.041
IPC2_protein 8.375
IPC_protein 8.404
Toseland 8.083
ProMoST 8.682
Dawson 8.858
Bjellqvist 9.165
Wikipedia 8.843
Rodwell 8.858
Grimsley 7.907
Solomon 9.033
Lehninger 9.019
Nozaki 9.268
DTASelect 8.902
Thurlkill 8.916
EMBOSS 9.019
Sillero 9.224
Patrickios 0.935
IPC_peptide 9.019
IPC2_peptide 8.682
IPC2.peptide.svr19 8.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1957
112
1298
391.4
43.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.249 ± 1.064
1.84 ± 0.779
4.65 ± 0.603
5.825 ± 0.8
4.139 ± 0.55
5.365 ± 1.279
3.577 ± 0.396
5.059 ± 0.341
5.161 ± 1.323
9.709 ± 1.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.402 ± 0.626
3.832 ± 0.417
6.745 ± 0.624
4.241 ± 0.617
4.803 ± 0.331
5.672 ± 0.548
7.001 ± 0.75
5.672 ± 0.738
1.175 ± 0.268
3.883 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here