Nibricoccus aquaticus
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A290QGX0|A0A290QGX0_9BACT Aminodeoxychorismate synthase OS=Nibricoccus aquaticus OX=2576891 GN=pabB PE=3 SV=1
MM1 pKa = 8.02 ADD3 pKa = 3.39 PNDD6 pKa = 3.7 RR7 pKa = 11.84 LTNNVAGRR15 pKa = 11.84 YY16 pKa = 8.68 YY17 pKa = 10.93 VDD19 pKa = 4.44 SSCVDD24 pKa = 3.42 CDD26 pKa = 3.15 QCRR29 pKa = 11.84 SHH31 pKa = 7.34 APAFFQRR38 pKa = 11.84 DD39 pKa = 3.64 EE40 pKa = 4.11 EE41 pKa = 4.69 TGFSFVFRR49 pKa = 11.84 QPASDD54 pKa = 3.84 EE55 pKa = 3.96 EE56 pKa = 4.61 VAVVEE61 pKa = 4.54 EE62 pKa = 4.73 ALDD65 pKa = 3.98 GCPTEE70 pKa = 5.58 SIGSDD75 pKa = 3.63 GSPP78 pKa = 3.05
Molecular weight: 8.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A290Q8I4|A0A290Q8I4_9BACT Efflux transporter periplasmic adaptor subunit OS=Nibricoccus aquaticus OX=2576891 GN=CMV30_12705 PE=3 SV=1
MM1 pKa = 7.59 GNLKK5 pKa = 10.05 KK6 pKa = 10.26 KK7 pKa = 10.28 RR8 pKa = 11.84 RR9 pKa = 11.84 LKK11 pKa = 9.46 MSKK14 pKa = 9.18 HH15 pKa = 5.32 KK16 pKa = 10.14 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.11 RR20 pKa = 11.84 LKK22 pKa = 10.0 ANRR25 pKa = 11.84 HH26 pKa = 5.49 KK27 pKa = 10.88 KK28 pKa = 8.96 RR29 pKa = 11.84 TWQKK33 pKa = 10.47
Molecular weight: 4.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3760
0
3760
1409849
33
3591
375.0
40.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.702 ± 0.051
0.838 ± 0.013
5.059 ± 0.026
5.617 ± 0.042
4.117 ± 0.021
7.769 ± 0.037
2.143 ± 0.021
4.912 ± 0.026
4.125 ± 0.037
10.316 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.851 ± 0.018
3.079 ± 0.028
5.3 ± 0.032
3.191 ± 0.021
6.693 ± 0.041
6.153 ± 0.038
6.226 ± 0.062
6.975 ± 0.032
1.523 ± 0.016
2.411 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here