Aquabacterium sp. SJQ9
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4667 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3R8T1B6|A0A3R8T1B6_9BURK Dioxygenase OS=Aquabacterium sp. SJQ9 OX=2493092 GN=EIP75_12915 PE=4 SV=1
MM1 pKa = 7.33 SAVPDD6 pKa = 4.79 PDD8 pKa = 4.01 PMPEE12 pKa = 4.22 PPEE15 pKa = 3.93 QPDD18 pKa = 3.12 INACCGSGCDD28 pKa = 3.4 PCIFDD33 pKa = 4.44 AHH35 pKa = 6.71 DD36 pKa = 3.89 LAMDD40 pKa = 5.16 DD41 pKa = 3.52 YY42 pKa = 10.9 RR43 pKa = 11.84 QALRR47 pKa = 11.84 AWQARR52 pKa = 11.84 QGDD55 pKa = 4.19 APSQGGG61 pKa = 3.24
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.516
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.757
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.681
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A3R8YQ86|A0A3R8YQ86_9BURK Amino acid ABC transporter permease OS=Aquabacterium sp. SJQ9 OX=2493092 GN=EIP75_04770 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.67 TKK25 pKa = 10.48 GGRR28 pKa = 11.84 KK29 pKa = 9.03 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.9 GRR39 pKa = 11.84 QRR41 pKa = 11.84 LAVV44 pKa = 3.51
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4667
0
4667
1626099
30
7900
348.4
37.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.409 ± 0.045
0.856 ± 0.012
5.468 ± 0.025
5.022 ± 0.041
3.233 ± 0.021
8.334 ± 0.053
2.416 ± 0.022
4.077 ± 0.026
3.15 ± 0.032
10.876 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.021
2.617 ± 0.033
5.364 ± 0.036
4.402 ± 0.025
6.812 ± 0.048
5.755 ± 0.039
5.486 ± 0.052
7.731 ± 0.032
1.566 ± 0.019
2.073 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here