Amino acid dipepetide frequency for Aquabacterium sp. SJQ9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.679AlaAla: 15.679 ± 0.145	1.241AlaCys: 1.241 ± 0.031	6.611AlaAsp: 6.611 ± 0.071	6.315AlaGlu: 6.315 ± 0.076	3.796AlaPhe: 3.796 ± 0.052	10.071AlaGly: 10.071 ± 0.134	2.883AlaHis: 2.883 ± 0.051	4.634AlaIle: 4.634 ± 0.063	3.658AlaLys: 3.658 ± 0.067	14.945AlaLeu: 14.945 ± 0.14	3.282AlaMet: 3.282 ± 0.05	2.768AlaAsn: 2.768 ± 0.057	6.088AlaPro: 6.088 ± 0.081	6.279AlaGln: 6.279 ± 0.079	8.66AlaArg: 8.66 ± 0.096	7.41AlaSer: 7.41 ± 0.082	6.302AlaThr: 6.302 ± 0.092	8.94AlaVal: 8.94 ± 0.09	2.123AlaTrp: 2.123 ± 0.044	2.4AlaTyr: 2.4 ± 0.043	0.002AlaXaa: 0.002 ± 0.001
Cys	1.029CysAla: 1.029 ± 0.025	0.108CysCys: 0.108 ± 0.008	0.467CysAsp: 0.467 ± 0.02	0.444CysGlu: 0.444 ± 0.017	0.261CysPhe: 0.261 ± 0.013	0.857CysGly: 0.857 ± 0.027	0.255CysHis: 0.255 ± 0.016	0.367CysIle: 0.367 ± 0.017	0.225CysLys: 0.225 ± 0.011	0.857CysLeu: 0.857 ± 0.024	0.19CysMet: 0.19 ± 0.012	0.2CysAsn: 0.2 ± 0.012	0.483CysPro: 0.483 ± 0.02	0.314CysGln: 0.314 ± 0.014	0.574CysArg: 0.574 ± 0.02	0.481CysSer: 0.481 ± 0.018	0.464CysThr: 0.464 ± 0.019	0.682CysVal: 0.682 ± 0.021	0.136CysTrp: 0.136 ± 0.009	0.17CysTyr: 0.17 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.944AspAla: 6.944 ± 0.084	0.424AspCys: 0.424 ± 0.017	3.027AspAsp: 3.027 ± 0.05	3.42AspGlu: 3.42 ± 0.057	1.9AspPhe: 1.9 ± 0.038	4.7AspGly: 4.7 ± 0.076	1.326AspHis: 1.326 ± 0.028	2.488AspIle: 2.488 ± 0.043	1.924AspLys: 1.924 ± 0.04	5.609AspLeu: 5.609 ± 0.065	1.186AspMet: 1.186 ± 0.029	1.33AspAsn: 1.33 ± 0.033	3.001AspPro: 3.001 ± 0.047	2.063AspGln: 2.063 ± 0.042	3.392AspArg: 3.392 ± 0.041	2.387AspSer: 2.387 ± 0.051	3.148AspThr: 3.148 ± 0.062	4.183AspVal: 4.183 ± 0.057	0.949AspTrp: 0.949 ± 0.023	1.278AspTyr: 1.278 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	6.881GluAla: 6.881 ± 0.087	0.359GluCys: 0.359 ± 0.014	2.192GluAsp: 2.192 ± 0.048	2.271GluGlu: 2.271 ± 0.042	1.542GluPhe: 1.542 ± 0.03	3.987GluGly: 3.987 ± 0.051	1.485GluHis: 1.485 ± 0.038	2.299GluIle: 2.299 ± 0.039	1.517GluLys: 1.517 ± 0.038	5.576GluLeu: 5.576 ± 0.061	1.09GluMet: 1.09 ± 0.03	1.068GluAsn: 1.068 ± 0.027	2.546GluPro: 2.546 ± 0.047	2.829GluGln: 2.829 ± 0.053	4.262GluArg: 4.262 ± 0.066	2.465GluSer: 2.465 ± 0.047	2.297GluThr: 2.297 ± 0.037	4.008GluVal: 4.008 ± 0.049	0.654GluTrp: 0.654 ± 0.026	0.891GluTyr: 0.891 ± 0.023	0.001GluXaa: 0.001 ± 0.001
Phe	3.504PheAla: 3.504 ± 0.042	0.344PheCys: 0.344 ± 0.016	2.373PheAsp: 2.373 ± 0.04	1.971PheGlu: 1.971 ± 0.035	1.221PhePhe: 1.221 ± 0.03	2.926PheGly: 2.926 ± 0.051	0.691PheHis: 0.691 ± 0.022	1.434PheIle: 1.434 ± 0.028	1.309PheLys: 1.309 ± 0.031	2.637PheLeu: 2.637 ± 0.046	0.82PheMet: 0.82 ± 0.024	1.159PheAsn: 1.159 ± 0.026	1.315PhePro: 1.315 ± 0.03	1.133PheGln: 1.133 ± 0.03	1.619PheArg: 1.619 ± 0.037	2.131PheSer: 2.131 ± 0.042	2.005PheThr: 2.005 ± 0.044	2.444PheVal: 2.444 ± 0.043	0.486PheTrp: 0.486 ± 0.02	0.805PheTyr: 0.805 ± 0.023	0.001PheXaa: 0.001 ± 0.001
Gly	8.843GlyAla: 8.843 ± 0.118	0.835GlyCys: 0.835 ± 0.026	4.203GlyAsp: 4.203 ± 0.067	4.05GlyGlu: 4.05 ± 0.056	3.038GlyPhe: 3.038 ± 0.044	7.078GlyGly: 7.078 ± 0.129	2.235GlyHis: 2.235 ± 0.043	3.589GlyIle: 3.589 ± 0.05	2.922GlyLys: 2.922 ± 0.05	9.56GlyLeu: 9.56 ± 0.093	2.197GlyMet: 2.197 ± 0.035	2.281GlyAsn: 2.281 ± 0.083	3.236GlyPro: 3.236 ± 0.046	4.008GlyGln: 4.008 ± 0.052	5.469GlyArg: 5.469 ± 0.065	4.885GlySer: 4.885 ± 0.105	4.767GlyThr: 4.767 ± 0.107	6.567GlyVal: 6.567 ± 0.067	1.526GlyTrp: 1.526 ± 0.031	2.05GlyTyr: 2.05 ± 0.04	0.002GlyXaa: 0.002 ± 0.001
His	3.004HisAla: 3.004 ± 0.056	0.255HisCys: 0.255 ± 0.013	1.318HisAsp: 1.318 ± 0.031	1.294HisGlu: 1.294 ± 0.035	0.879HisPhe: 0.879 ± 0.026	2.297HisGly: 2.297 ± 0.042	0.81HisHis: 0.81 ± 0.028	0.91HisIle: 0.91 ± 0.026	0.613HisLys: 0.613 ± 0.019	2.537HisLeu: 2.537 ± 0.045	0.502HisMet: 0.502 ± 0.017	0.507HisAsn: 0.507 ± 0.02	1.582HisPro: 1.582 ± 0.034	0.937HisGln: 0.937 ± 0.024	1.662HisArg: 1.662 ± 0.031	1.068HisSer: 1.068 ± 0.025	1.203HisThr: 1.203 ± 0.025	1.713HisVal: 1.713 ± 0.031	0.482HisTrp: 0.482 ± 0.019	0.589HisTyr: 0.589 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.08IleAla: 5.08 ± 0.063	0.304IleCys: 0.304 ± 0.016	3.149IleAsp: 3.149 ± 0.048	3.036IleGlu: 3.036 ± 0.05	1.084IlePhe: 1.084 ± 0.032	3.811IleGly: 3.811 ± 0.064	0.836IleHis: 0.836 ± 0.021	1.433IleIle: 1.433 ± 0.028	1.588IleLys: 1.588 ± 0.039	3.072IleLeu: 3.072 ± 0.05	0.701IleMet: 0.701 ± 0.021	1.504IleAsn: 1.504 ± 0.035	1.852IlePro: 1.852 ± 0.034	1.367IleGln: 1.367 ± 0.025	2.411IleArg: 2.411 ± 0.043	2.374IleSer: 2.374 ± 0.037	2.758IleThr: 2.758 ± 0.061	3.169IleVal: 3.169 ± 0.045	0.41IleTrp: 0.41 ± 0.018	0.834IleTyr: 0.834 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	4.403LysAla: 4.403 ± 0.065	0.146LysCys: 0.146 ± 0.01	1.733LysAsp: 1.733 ± 0.04	1.422LysGlu: 1.422 ± 0.04	0.829LysPhe: 0.829 ± 0.023	2.548LysGly: 2.548 ± 0.048	0.668LysHis: 0.668 ± 0.022	1.258LysIle: 1.258 ± 0.033	1.242LysLys: 1.242 ± 0.047	3.331LysLeu: 3.331 ± 0.059	0.672LysMet: 0.672 ± 0.024	0.84LysAsn: 0.84 ± 0.027	2.142LysPro: 2.142 ± 0.049	1.271LysGln: 1.271 ± 0.03	2.08LysArg: 2.08 ± 0.038	1.644LysSer: 1.644 ± 0.032	1.801LysThr: 1.801 ± 0.034	2.594LysVal: 2.594 ± 0.045	0.311LysTrp: 0.311 ± 0.014	0.561LysTyr: 0.561 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.264LeuAla: 14.264 ± 0.111	0.969LeuCys: 0.969 ± 0.025	6.297LeuAsp: 6.297 ± 0.066	4.84LeuGlu: 4.84 ± 0.067	3.162LeuPhe: 3.162 ± 0.048	8.95LeuGly: 8.95 ± 0.081	2.419LeuHis: 2.419 ± 0.042	4.256LeuIle: 4.256 ± 0.059	3.854LeuLys: 3.854 ± 0.06	11.096LeuLeu: 11.096 ± 0.114	2.612LeuMet: 2.612 ± 0.047	2.925LeuAsn: 2.925 ± 0.044	6.322LeuPro: 6.322 ± 0.068	4.416LeuGln: 4.416 ± 0.056	7.756LeuArg: 7.756 ± 0.078	7.237LeuSer: 7.237 ± 0.089	5.823LeuThr: 5.823 ± 0.078	8.037LeuVal: 8.037 ± 0.081	1.506LeuTrp: 1.506 ± 0.037	2.02LeuTyr: 2.02 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	3.125MetAla: 3.125 ± 0.052	0.156MetCys: 0.156 ± 0.009	1.239MetAsp: 1.239 ± 0.03	0.937MetGlu: 0.937 ± 0.026	0.649MetPhe: 0.649 ± 0.021	1.881MetGly: 1.881 ± 0.038	0.522MetHis: 0.522 ± 0.019	0.836MetIle: 0.836 ± 0.023	0.872MetLys: 0.872 ± 0.023	2.498MetLeu: 2.498 ± 0.049	0.534MetMet: 0.534 ± 0.018	0.74MetAsn: 0.74 ± 0.023	1.526MetPro: 1.526 ± 0.034	0.998MetGln: 0.998 ± 0.027	1.553MetArg: 1.553 ± 0.035	1.758MetSer: 1.758 ± 0.034	1.4MetThr: 1.4 ± 0.03	1.767MetVal: 1.767 ± 0.035	0.214MetTrp: 0.214 ± 0.011	0.322MetTyr: 0.322 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.269AsnAla: 3.269 ± 0.065	0.212AsnCys: 0.212 ± 0.012	1.464AsnAsp: 1.464 ± 0.052	1.219AsnGlu: 1.219 ± 0.025	0.861AsnPhe: 0.861 ± 0.026	2.385AsnGly: 2.385 ± 0.058	0.552AsnHis: 0.552 ± 0.019	1.119AsnIle: 1.119 ± 0.032	0.811AsnLys: 0.811 ± 0.025	2.655AsnLeu: 2.655 ± 0.047	0.516AsnMet: 0.516 ± 0.016	0.854AsnAsn: 0.854 ± 0.035	1.707AsnPro: 1.707 ± 0.035	1.068AsnGln: 1.068 ± 0.026	1.608AsnArg: 1.608 ± 0.032	1.227AsnSer: 1.227 ± 0.037	1.64AsnThr: 1.64 ± 0.049	1.986AsnVal: 1.986 ± 0.045	0.391AsnTrp: 0.391 ± 0.017	0.623AsnTyr: 0.623 ± 0.021	0.001AsnXaa: 0.001 ± 0.001
Pro	6.979ProAla: 6.979 ± 0.087	0.403ProCys: 0.403 ± 0.017	3.347ProAsp: 3.347 ± 0.047	3.216ProGlu: 3.216 ± 0.047	1.612ProPhe: 1.612 ± 0.031	4.687ProGly: 4.687 ± 0.062	1.189ProHis: 1.189 ± 0.028	1.875ProIle: 1.875 ± 0.036	1.526ProLys: 1.526 ± 0.033	5.423ProLeu: 5.423 ± 0.076	1.35ProMet: 1.35 ± 0.026	1.277ProAsn: 1.277 ± 0.03	2.946ProPro: 2.946 ± 0.059	2.208ProGln: 2.208 ± 0.04	3.037ProArg: 3.037 ± 0.053	3.297ProSer: 3.297 ± 0.056	2.818ProThr: 2.818 ± 0.042	4.471ProVal: 4.471 ± 0.065	0.924ProTrp: 0.924 ± 0.028	1.054ProTyr: 1.054 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	7.186GlnAla: 7.186 ± 0.086	0.307GlnCys: 0.307 ± 0.014	1.958GlnAsp: 1.958 ± 0.036	1.692GlnGlu: 1.692 ± 0.033	1.197GlnPhe: 1.197 ± 0.029	3.946GlnGly: 3.946 ± 0.059	1.084GlnHis: 1.084 ± 0.027	1.804GlnIle: 1.804 ± 0.044	1.02GlnLys: 1.02 ± 0.03	4.343GlnLeu: 4.343 ± 0.058	0.95GlnMet: 0.95 ± 0.026	0.804GlnAsn: 0.804 ± 0.023	2.564GlnPro: 2.564 ± 0.041	2.241GlnGln: 2.241 ± 0.045	3.652GlnArg: 3.652 ± 0.057	2.171GlnSer: 2.171 ± 0.042	2.12GlnThr: 2.12 ± 0.035	3.47GlnVal: 3.47 ± 0.063	0.735GlnTrp: 0.735 ± 0.021	0.774GlnTyr: 0.774 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	7.306ArgAla: 7.306 ± 0.088	0.587ArgCys: 0.587 ± 0.02	3.556ArgAsp: 3.556 ± 0.05	3.866ArgGlu: 3.866 ± 0.062	2.658ArgPhe: 2.658 ± 0.045	4.441ArgGly: 4.441 ± 0.062	2.086ArgHis: 2.086 ± 0.039	3.078ArgIle: 3.078 ± 0.04	1.957ArgLys: 1.957 ± 0.045	8.372ArgLeu: 8.372 ± 0.099	1.7ArgMet: 1.7 ± 0.032	1.686ArgAsn: 1.686 ± 0.031	3.382ArgPro: 3.382 ± 0.058	3.526ArgGln: 3.526 ± 0.053	5.095ArgArg: 5.095 ± 0.075	3.412ArgSer: 3.412 ± 0.051	3.298ArgThr: 3.298 ± 0.044	5.071ArgVal: 5.071 ± 0.059	1.342ArgTrp: 1.342 ± 0.035	1.701ArgTyr: 1.701 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	6.789SerAla: 6.789 ± 0.079	0.427SerCys: 0.427 ± 0.02	2.839SerAsp: 2.839 ± 0.051	2.502SerGlu: 2.502 ± 0.04	2.018SerPhe: 2.018 ± 0.043	5.268SerGly: 5.268 ± 0.087	1.342SerHis: 1.342 ± 0.033	2.504SerIle: 2.504 ± 0.05	1.563SerLys: 1.563 ± 0.037	6.463SerLeu: 6.463 ± 0.075	1.39SerMet: 1.39 ± 0.029	1.533SerAsn: 1.533 ± 0.036	3.217SerPro: 3.217 ± 0.046	2.306SerGln: 2.306 ± 0.042	3.769SerArg: 3.769 ± 0.052	3.698SerSer: 3.698 ± 0.072	3.601SerThr: 3.601 ± 0.08	4.273SerVal: 4.273 ± 0.07	0.833SerTrp: 0.833 ± 0.021	1.21SerTyr: 1.21 ± 0.029	0.001SerXaa: 0.001 ± 0.001
Thr	6.45ThrAla: 6.45 ± 0.106	0.443ThrCys: 0.443 ± 0.019	2.805ThrAsp: 2.805 ± 0.05	2.433ThrGlu: 2.433 ± 0.034	1.7ThrPhe: 1.7 ± 0.036	4.933ThrGly: 4.933 ± 0.108	1.19ThrHis: 1.19 ± 0.029	2.177ThrIle: 2.177 ± 0.05	1.322ThrLys: 1.322 ± 0.027	6.64ThrLeu: 6.64 ± 0.076	1.055ThrMet: 1.055 ± 0.027	1.333ThrAsn: 1.333 ± 0.04	3.735ThrPro: 3.735 ± 0.059	2.25ThrGln: 2.25 ± 0.041	3.364ThrArg: 3.364 ± 0.052	3.119ThrSer: 3.119 ± 0.065	3.204ThrThr: 3.204 ± 0.081	4.74ThrVal: 4.74 ± 0.084	0.8ThrTrp: 0.8 ± 0.023	1.167ThrTyr: 1.167 ± 0.027	0.002ThrXaa: 0.002 ± 0.001
Val	9.371ValAla: 9.371 ± 0.096	0.679ValCys: 0.679 ± 0.022	4.263ValAsp: 4.263 ± 0.059	3.704ValGlu: 3.704 ± 0.053	2.584ValPhe: 2.584 ± 0.041	5.651ValGly: 5.651 ± 0.068	1.756ValHis: 1.756 ± 0.035	3.359ValIle: 3.359 ± 0.048	2.462ValLys: 2.462 ± 0.049	8.701ValLeu: 8.701 ± 0.075	1.927ValMet: 1.927 ± 0.038	2.352ValAsn: 2.352 ± 0.054	4.176ValPro: 4.176 ± 0.066	3.082ValGln: 3.082 ± 0.05	5.188ValArg: 5.188 ± 0.067	4.744ValSer: 4.744 ± 0.067	4.179ValThr: 4.179 ± 0.072	6.535ValVal: 6.535 ± 0.072	1.083ValTrp: 1.083 ± 0.027	1.516ValTyr: 1.516 ± 0.036	0.001ValXaa: 0.001 ± 0.001
Trp	1.622TrpAla: 1.622 ± 0.038	0.173TrpCys: 0.173 ± 0.012	0.638TrpAsp: 0.638 ± 0.019	0.486TrpGlu: 0.486 ± 0.016	0.528TrpPhe: 0.528 ± 0.019	1.098TrpGly: 1.098 ± 0.028	0.413TrpHis: 0.413 ± 0.017	0.596TrpIle: 0.596 ± 0.019	0.461TrpLys: 0.461 ± 0.017	2.2TrpLeu: 2.2 ± 0.047	0.462TrpMet: 0.462 ± 0.015	0.384TrpAsn: 0.384 ± 0.015	0.834TrpPro: 0.834 ± 0.025	0.827TrpGln: 0.827 ± 0.026	1.402TrpArg: 1.402 ± 0.037	0.923TrpSer: 0.923 ± 0.025	0.822TrpThr: 0.822 ± 0.025	1.128TrpVal: 1.128 ± 0.028	0.376TrpTrp: 0.376 ± 0.017	0.284TrpTyr: 0.284 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.359TyrAla: 2.359 ± 0.042	0.194TyrCys: 0.194 ± 0.012	1.198TyrAsp: 1.198 ± 0.03	1.1TyrGlu: 1.1 ± 0.025	0.806TyrPhe: 0.806 ± 0.025	1.825TyrGly: 1.825 ± 0.04	0.419TyrHis: 0.419 ± 0.015	0.756TyrIle: 0.756 ± 0.025	0.651TyrLys: 0.651 ± 0.021	2.245TyrLeu: 2.245 ± 0.041	0.39TyrMet: 0.39 ± 0.016	0.622TyrAsn: 0.622 ± 0.022	0.984TyrPro: 0.984 ± 0.026	0.899TyrGln: 0.899 ± 0.023	1.563TyrArg: 1.563 ± 0.031	1.115TyrSer: 1.115 ± 0.026	1.212TyrThr: 1.212 ± 0.033	1.533TyrVal: 1.533 ± 0.03	0.376TyrTrp: 0.376 ± 0.015	0.488TyrTyr: 0.488 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4667 proteins (1626099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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