Streptococcus phage Javan367

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A998|A0A4D6A998_9CAUD Uncharacterized protein OS=Streptococcus phage Javan367 OX=2548128 GN=Javan367_0045 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.58IEE5 pKa = 4.44LLDD8 pKa = 4.06SYY10 pKa = 11.45EE11 pKa = 4.43DD12 pKa = 3.44LLNYY16 pKa = 9.99VEE18 pKa = 5.18EE19 pKa = 4.47IRR21 pKa = 11.84EE22 pKa = 4.16SMDD25 pKa = 5.7LIHH28 pKa = 6.61NWLAKK33 pKa = 10.36EE34 pKa = 4.23PDD36 pKa = 3.31WDD38 pKa = 4.3SQCEE42 pKa = 4.25LYY44 pKa = 11.05DD45 pKa = 5.13FIAQHH50 pKa = 6.19SSQFAVLNLIMYY62 pKa = 10.2RR63 pKa = 11.84LDD65 pKa = 3.49SLRR68 pKa = 11.84DD69 pKa = 3.3EE70 pKa = 4.63HH71 pKa = 7.41RR72 pKa = 11.84AIIDD76 pKa = 3.51NYY78 pKa = 10.17IKK80 pKa = 10.84GVV82 pKa = 3.33

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A844|A0A4D6A844_9CAUD Uncharacterized protein OS=Streptococcus phage Javan367 OX=2548128 GN=Javan367_0016 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.65PKK4 pKa = 10.2KK5 pKa = 9.78YY6 pKa = 9.62PYY8 pKa = 9.87SGRR11 pKa = 11.84QGLTKK16 pKa = 10.17TGLPRR21 pKa = 11.84FIQLGNIAIDD31 pKa = 3.42SKK33 pKa = 10.96FINNIEE39 pKa = 3.97MFEE42 pKa = 4.12WVGPNEE48 pKa = 4.37TVIHH52 pKa = 5.63LRR54 pKa = 11.84VPKK57 pKa = 10.37FFAYY61 pKa = 9.68EE62 pKa = 4.0EE63 pKa = 4.16KK64 pKa = 10.79QITVQLKK71 pKa = 9.49LGQVLKK77 pKa = 10.69ILNRR81 pKa = 11.84YY82 pKa = 7.78

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11343

38

1399

210.1

23.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.106 ± 0.715

0.652 ± 0.141

6.48 ± 0.378

8.49 ± 0.372

3.967 ± 0.295

5.81 ± 0.337

1.322 ± 0.159

6.63 ± 0.262

9.054 ± 0.349

8.349 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.183

5.254 ± 0.247

2.636 ± 0.181

4.443 ± 0.204

4.337 ± 0.235

6.171 ± 0.32

5.669 ± 0.283

5.96 ± 0.228

1.137 ± 0.118

3.844 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski