Colwellia phage 9A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Franklinbayvirus; Colwellia virus 9A

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3UM93|I3UM93_9CAUD Uncharacterized protein OS=Colwellia phage 9A OX=765765 GN=COPG_00012 PE=4 SV=1
MM1 pKa = 7.38TIEE4 pKa = 4.7NITAIFKK11 pKa = 10.19EE12 pKa = 4.69LEE14 pKa = 4.26TSIHH18 pKa = 6.32TINPVLSYY26 pKa = 11.59DD27 pKa = 4.18DD28 pKa = 3.86IAPKK32 pKa = 9.33FTALCEE38 pKa = 4.4AIEE41 pKa = 4.48AFTGDD46 pKa = 3.69TEE48 pKa = 4.2EE49 pKa = 3.57WCYY52 pKa = 10.55IGEE55 pKa = 4.47HH56 pKa = 6.85GYY58 pKa = 10.83CCIDD62 pKa = 4.14DD63 pKa = 4.42LLIGAFWHH71 pKa = 5.17FTEE74 pKa = 3.93WHH76 pKa = 6.53KK77 pKa = 11.17GQSSLSYY84 pKa = 10.48AALSALGGIYY94 pKa = 9.32TPNMEE99 pKa = 5.15CAPTDD104 pKa = 3.5QDD106 pKa = 4.59DD107 pKa = 4.35SGFDD111 pKa = 3.49TYY113 pKa = 12.01DD114 pKa = 3.74LLNDD118 pKa = 4.49LATAFNKK125 pKa = 10.16KK126 pKa = 9.57

Molecular weight: 14.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3UM97|I3UM97_9CAUD Nicotinamide phosphoribosyl transferase OS=Colwellia phage 9A OX=765765 GN=COPG_00016 PE=3 SV=1
MM1 pKa = 7.57GMNKK5 pKa = 9.11VKK7 pKa = 10.5KK8 pKa = 9.96QYY10 pKa = 10.7TYY12 pKa = 11.31FLRR15 pKa = 11.84NSHH18 pKa = 6.23NYY20 pKa = 8.36IKK22 pKa = 9.9KK23 pKa = 7.31TLEE26 pKa = 3.89YY27 pKa = 10.79NFEE30 pKa = 4.35TNQEE34 pKa = 4.26EE35 pKa = 4.41VWEE38 pKa = 4.05IVYY41 pKa = 10.22NQTCTVRR48 pKa = 11.84VQAYY52 pKa = 9.33EE53 pKa = 4.35NITMGQAKK61 pKa = 10.22RR62 pKa = 11.84ILRR65 pKa = 11.84NHH67 pKa = 6.03RR68 pKa = 11.84MGWHH72 pKa = 6.55PRR74 pKa = 11.84RR75 pKa = 11.84CGMGGRR81 pKa = 11.84KK82 pKa = 9.39LKK84 pKa = 10.99DD85 pKa = 2.98KK86 pKa = 10.23MYY88 pKa = 10.63ISGGCNINYY97 pKa = 9.41DD98 pKa = 3.27NRR100 pKa = 11.84TRR102 pKa = 11.84RR103 pKa = 11.84LTIRR107 pKa = 11.84VPFNKK112 pKa = 9.71IKK114 pKa = 10.47ILSKK118 pKa = 10.35EE119 pKa = 3.84IFVKK123 pKa = 10.55KK124 pKa = 10.25EE125 pKa = 3.98KK126 pKa = 10.55VSLWW130 pKa = 3.34

Molecular weight: 15.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

31709

49

1710

212.8

23.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.78 ± 0.312

1.369 ± 0.108

6.462 ± 0.153

6.418 ± 0.207

4.075 ± 0.126

6.579 ± 0.178

2.176 ± 0.111

6.686 ± 0.147

7.534 ± 0.267

8.401 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.128

5.412 ± 0.169

3.229 ± 0.124

3.422 ± 0.13

4.191 ± 0.179

6.642 ± 0.195

6.348 ± 0.245

6.657 ± 0.223

1.227 ± 0.078

3.687 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski