Amino acid dipepetide frequency for Colwellia phage 9A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.803AlaAla: 5.803 ± 0.679
0.788AlaCys: 0.788 ± 0.186
3.532AlaAsp: 3.532 ± 0.393
4.951AlaGlu: 4.951 ± 0.469
2.46AlaPhe: 2.46 ± 0.33
3.595AlaGly: 3.595 ± 0.451
0.852AlaHis: 0.852 ± 0.177
5.141AlaIle: 5.141 ± 0.427
5.298AlaLys: 5.298 ± 0.466
6.781AlaLeu: 6.781 ± 0.594
1.829AlaMet: 1.829 ± 0.252
3.658AlaAsn: 3.658 ± 0.413
1.798AlaPro: 1.798 ± 0.258
2.208AlaGln: 2.208 ± 0.266
2.807AlaArg: 2.807 ± 0.304
4.541AlaSer: 4.541 ± 0.464
4.005AlaThr: 4.005 ± 0.434
4.447AlaVal: 4.447 ± 0.381
0.915AlaTrp: 0.915 ± 0.146
2.397AlaTyr: 2.397 ± 0.243
0.0AlaXaa: 0.0 ± 0.0
Cys
0.505CysAla: 0.505 ± 0.118
0.252CysCys: 0.252 ± 0.096
0.883CysAsp: 0.883 ± 0.177
0.852CysGlu: 0.852 ± 0.165
0.442CysPhe: 0.442 ± 0.12
1.135CysGly: 1.135 ± 0.198
0.442CysHis: 0.442 ± 0.143
0.599CysIle: 0.599 ± 0.173
1.198CysLys: 1.198 ± 0.208
1.072CysLeu: 1.072 ± 0.2
0.378CysMet: 0.378 ± 0.138
0.852CysAsn: 0.852 ± 0.148
0.473CysPro: 0.473 ± 0.127
0.536CysGln: 0.536 ± 0.147
0.599CysArg: 0.599 ± 0.171
0.788CysSer: 0.788 ± 0.163
1.104CysThr: 1.104 ± 0.195
0.852CysVal: 0.852 ± 0.17
0.189CysTrp: 0.189 ± 0.105
0.536CysTyr: 0.536 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
4.573AspAla: 4.573 ± 0.483
0.915AspCys: 0.915 ± 0.196
3.375AspAsp: 3.375 ± 0.383
3.785AspGlu: 3.785 ± 0.393
2.87AspPhe: 2.87 ± 0.366
5.298AspGly: 5.298 ± 0.466
1.419AspHis: 1.419 ± 0.217
4.573AspIle: 4.573 ± 0.37
4.825AspLys: 4.825 ± 0.409
5.551AspLeu: 5.551 ± 0.372
1.545AspMet: 1.545 ± 0.22
3.343AspAsn: 3.343 ± 0.286
2.712AspPro: 2.712 ± 0.347
2.428AspGln: 2.428 ± 0.263
2.176AspArg: 2.176 ± 0.293
4.226AspSer: 4.226 ± 0.302
4.226AspThr: 4.226 ± 0.41
3.564AspVal: 3.564 ± 0.398
0.978AspTrp: 0.978 ± 0.241
2.239AspTyr: 2.239 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
4.573GluAla: 4.573 ± 0.495
0.915GluCys: 0.915 ± 0.185
4.857GluAsp: 4.857 ± 0.55
3.753GluGlu: 3.753 ± 0.446
3.438GluPhe: 3.438 ± 0.347
4.1GluGly: 4.1 ± 0.309
1.262GluHis: 1.262 ± 0.249
4.289GluIle: 4.289 ± 0.374
4.605GluLys: 4.605 ± 0.432
6.213GluLeu: 6.213 ± 0.511
1.955GluMet: 1.955 ± 0.304
3.343GluAsn: 3.343 ± 0.328
1.451GluPro: 1.451 ± 0.202
2.176GluGln: 2.176 ± 0.333
2.491GluArg: 2.491 ± 0.282
3.658GluSer: 3.658 ± 0.37
3.721GluThr: 3.721 ± 0.437
4.352GluVal: 4.352 ± 0.333
0.725GluTrp: 0.725 ± 0.173
2.302GluTyr: 2.302 ± 0.242
0.0GluXaa: 0.0 ± 0.0
Phe
2.113PheAla: 2.113 ± 0.26
0.788PheCys: 0.788 ± 0.157
3.311PheAsp: 3.311 ± 0.321
2.901PheGlu: 2.901 ± 0.271
1.577PhePhe: 1.577 ± 0.225
2.334PheGly: 2.334 ± 0.267
0.946PheHis: 0.946 ± 0.16
2.965PheIle: 2.965 ± 0.322
3.122PheLys: 3.122 ± 0.256
2.996PheLeu: 2.996 ± 0.311
1.104PheMet: 1.104 ± 0.188
2.649PheAsn: 2.649 ± 0.306
1.104PhePro: 1.104 ± 0.182
1.135PheGln: 1.135 ± 0.173
1.703PheArg: 1.703 ± 0.194
2.996PheSer: 2.996 ± 0.273
2.586PheThr: 2.586 ± 0.28
2.365PheVal: 2.365 ± 0.249
0.473PheTrp: 0.473 ± 0.132
1.577PheTyr: 1.577 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
4.226GlyAla: 4.226 ± 0.35
1.072GlyCys: 1.072 ± 0.174
4.195GlyAsp: 4.195 ± 0.333
3.469GlyGlu: 3.469 ± 0.325
2.901GlyPhe: 2.901 ± 0.328
5.961GlyGly: 5.961 ± 0.582
1.23GlyHis: 1.23 ± 0.192
5.078GlyIle: 5.078 ± 0.445
5.235GlyLys: 5.235 ± 0.376
5.015GlyLeu: 5.015 ± 0.466
1.608GlyMet: 1.608 ± 0.214
3.532GlyAsn: 3.532 ± 0.269
0.063GlyPro: 0.063 ± 0.044
1.892GlyGln: 1.892 ± 0.278
2.586GlyArg: 2.586 ± 0.319
4.258GlySer: 4.258 ± 0.364
4.888GlyThr: 4.888 ± 0.541
4.226GlyVal: 4.226 ± 0.443
0.915GlyTrp: 0.915 ± 0.178
3.438GlyTyr: 3.438 ± 0.311
0.0GlyXaa: 0.0 ± 0.0
His
1.041HisAla: 1.041 ± 0.197
0.631HisCys: 0.631 ± 0.159
1.482HisAsp: 1.482 ± 0.223
1.104HisGlu: 1.104 ± 0.178
0.915HisPhe: 0.915 ± 0.163
1.419HisGly: 1.419 ± 0.232
0.599HisHis: 0.599 ± 0.135
1.356HisIle: 1.356 ± 0.22
1.451HisLys: 1.451 ± 0.218
2.113HisLeu: 2.113 ± 0.264
0.505HisMet: 0.505 ± 0.137
1.041HisAsn: 1.041 ± 0.17
0.757HisPro: 0.757 ± 0.17
0.631HisGln: 0.631 ± 0.135
1.198HisArg: 1.198 ± 0.177
1.64HisSer: 1.64 ± 0.212
1.514HisThr: 1.514 ± 0.211
1.325HisVal: 1.325 ± 0.22
0.315HisTrp: 0.315 ± 0.094
0.694HisTyr: 0.694 ± 0.123
0.0HisXaa: 0.0 ± 0.0
Ile
4.163IleAla: 4.163 ± 0.399
0.631IleCys: 0.631 ± 0.173
5.204IleAsp: 5.204 ± 0.492
5.046IleGlu: 5.046 ± 0.421
2.239IlePhe: 2.239 ± 0.25
4.131IleGly: 4.131 ± 0.347
1.325IleHis: 1.325 ± 0.236
4.131IleIle: 4.131 ± 0.385
5.172IleLys: 5.172 ± 0.465
5.298IleLeu: 5.298 ± 0.454
1.829IleMet: 1.829 ± 0.224
3.942IleAsn: 3.942 ± 0.311
2.523IlePro: 2.523 ± 0.239
2.081IleGln: 2.081 ± 0.264
2.965IleArg: 2.965 ± 0.253
4.289IleSer: 4.289 ± 0.395
4.951IleThr: 4.951 ± 0.387
3.879IleVal: 3.879 ± 0.378
0.788IleTrp: 0.788 ± 0.148
2.271IleTyr: 2.271 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
5.519LysAla: 5.519 ± 0.649
0.757LysCys: 0.757 ± 0.177
4.352LysAsp: 4.352 ± 0.31
5.519LysGlu: 5.519 ± 0.495
2.775LysPhe: 2.775 ± 0.276
4.541LysGly: 4.541 ± 0.456
2.081LysHis: 2.081 ± 0.205
4.541LysIle: 4.541 ± 0.376
5.015LysLys: 5.015 ± 0.52
6.844LysLeu: 6.844 ± 0.521
2.428LysMet: 2.428 ± 0.29
3.627LysAsn: 3.627 ± 0.331
3.185LysPro: 3.185 ± 0.389
2.996LysGln: 2.996 ± 0.349
3.911LysArg: 3.911 ± 0.353
4.068LysSer: 4.068 ± 0.401
4.573LysThr: 4.573 ± 0.311
5.141LysVal: 5.141 ± 0.428
1.041LysTrp: 1.041 ± 0.164
2.428LysTyr: 2.428 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
5.456LeuAla: 5.456 ± 0.447
1.009LeuCys: 1.009 ± 0.201
5.235LeuAsp: 5.235 ± 0.362
6.56LeuGlu: 6.56 ± 0.474
3.217LeuPhe: 3.217 ± 0.321
4.951LeuGly: 4.951 ± 0.448
1.482LeuHis: 1.482 ± 0.199
5.235LeuIle: 5.235 ± 0.434
7.096LeuLys: 7.096 ± 0.609
6.528LeuLeu: 6.528 ± 0.501
2.239LeuMet: 2.239 ± 0.295
4.699LeuAsn: 4.699 ± 0.522
3.185LeuPro: 3.185 ± 0.317
3.154LeuGln: 3.154 ± 0.364
3.501LeuArg: 3.501 ± 0.368
6.244LeuSer: 6.244 ± 0.473
5.74LeuThr: 5.74 ± 0.379
5.424LeuVal: 5.424 ± 0.428
0.505LeuTrp: 0.505 ± 0.128
2.555LeuTyr: 2.555 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
2.239MetAla: 2.239 ± 0.281
0.378MetCys: 0.378 ± 0.102
1.577MetAsp: 1.577 ± 0.253
1.262MetGlu: 1.262 ± 0.217
0.883MetPhe: 0.883 ± 0.156
1.829MetGly: 1.829 ± 0.263
0.378MetHis: 0.378 ± 0.108
1.987MetIle: 1.987 ± 0.221
2.239MetLys: 2.239 ± 0.301
2.113MetLeu: 2.113 ± 0.334
0.442MetMet: 0.442 ± 0.121
1.672MetAsn: 1.672 ± 0.242
1.041MetPro: 1.041 ± 0.185
0.946MetGln: 0.946 ± 0.184
1.135MetArg: 1.135 ± 0.2
2.365MetSer: 2.365 ± 0.317
1.829MetThr: 1.829 ± 0.239
1.577MetVal: 1.577 ± 0.236
0.189MetTrp: 0.189 ± 0.068
0.978MetTyr: 0.978 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.595AsnAla: 3.595 ± 0.345
0.505AsnCys: 0.505 ± 0.131
3.406AsnAsp: 3.406 ± 0.348
2.681AsnGlu: 2.681 ± 0.304
2.113AsnPhe: 2.113 ± 0.25
4.068AsnGly: 4.068 ± 0.385
1.198AsnHis: 1.198 ± 0.204
3.501AsnIle: 3.501 ± 0.299
4.447AsnLys: 4.447 ± 0.433
4.131AsnLeu: 4.131 ± 0.305
1.514AsnMet: 1.514 ± 0.259
3.059AsnAsn: 3.059 ± 0.419
2.302AsnPro: 2.302 ± 0.293
1.703AsnGln: 1.703 ± 0.251
2.586AsnArg: 2.586 ± 0.248
3.816AsnSer: 3.816 ± 0.358
3.185AsnThr: 3.185 ± 0.385
3.658AsnVal: 3.658 ± 0.319
0.662AsnTrp: 0.662 ± 0.136
1.987AsnTyr: 1.987 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
1.64ProAla: 1.64 ± 0.231
0.347ProCys: 0.347 ± 0.104
2.523ProAsp: 2.523 ± 0.291
2.933ProGlu: 2.933 ± 0.348
1.545ProPhe: 1.545 ± 0.219
0.0ProGly: 0.0 ± 0.0
0.725ProHis: 0.725 ± 0.166
2.081ProIle: 2.081 ± 0.226
1.987ProLys: 1.987 ± 0.259
2.87ProLeu: 2.87 ± 0.321
0.946ProMet: 0.946 ± 0.148
2.113ProAsn: 2.113 ± 0.301
0.883ProPro: 0.883 ± 0.198
1.009ProGln: 1.009 ± 0.196
1.356ProArg: 1.356 ± 0.185
2.523ProSer: 2.523 ± 0.294
2.744ProThr: 2.744 ± 0.363
2.901ProVal: 2.901 ± 0.403
0.315ProTrp: 0.315 ± 0.096
0.852ProTyr: 0.852 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
2.775GlnAla: 2.775 ± 0.324
0.505GlnCys: 0.505 ± 0.128
2.018GlnAsp: 2.018 ± 0.226
2.081GlnGlu: 2.081 ± 0.313
1.545GlnPhe: 1.545 ± 0.213
1.703GlnGly: 1.703 ± 0.253
0.757GlnHis: 0.757 ± 0.159
2.05GlnIle: 2.05 ± 0.274
2.397GlnLys: 2.397 ± 0.332
3.564GlnLeu: 3.564 ± 0.31
1.356GlnMet: 1.356 ± 0.212
1.325GlnAsn: 1.325 ± 0.225
1.167GlnPro: 1.167 ± 0.195
1.167GlnGln: 1.167 ± 0.186
1.135GlnArg: 1.135 ± 0.161
1.766GlnSer: 1.766 ± 0.236
1.64GlnThr: 1.64 ± 0.239
3.185GlnVal: 3.185 ± 0.332
0.536GlnTrp: 0.536 ± 0.118
1.545GlnTyr: 1.545 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
2.113ArgAla: 2.113 ± 0.27
0.631ArgCys: 0.631 ± 0.112
2.586ArgAsp: 2.586 ± 0.315
3.091ArgGlu: 3.091 ± 0.317
1.861ArgPhe: 1.861 ± 0.262
2.491ArgGly: 2.491 ± 0.338
1.009ArgHis: 1.009 ± 0.167
2.555ArgIle: 2.555 ± 0.218
4.037ArgLys: 4.037 ± 0.412
3.911ArgLeu: 3.911 ± 0.364
1.262ArgMet: 1.262 ± 0.215
1.987ArgAsn: 1.987 ± 0.244
1.167ArgPro: 1.167 ± 0.165
1.325ArgGln: 1.325 ± 0.228
1.892ArgArg: 1.892 ± 0.294
2.807ArgSer: 2.807 ± 0.324
1.892ArgThr: 1.892 ± 0.278
2.807ArgVal: 2.807 ± 0.226
0.536ArgTrp: 0.536 ± 0.12
1.955ArgTyr: 1.955 ± 0.246
0.0ArgXaa: 0.0 ± 0.0
Ser
4.541SerAla: 4.541 ± 0.429
1.009SerCys: 1.009 ± 0.179
3.942SerAsp: 3.942 ± 0.361
3.469SerGlu: 3.469 ± 0.376
2.618SerPhe: 2.618 ± 0.282
5.898SerGly: 5.898 ± 0.476
1.482SerHis: 1.482 ± 0.198
4.541SerIle: 4.541 ± 0.353
4.478SerLys: 4.478 ± 0.359
5.708SerLeu: 5.708 ± 0.498
1.672SerMet: 1.672 ± 0.199
3.658SerAsn: 3.658 ± 0.338
2.334SerPro: 2.334 ± 0.336
2.712SerGln: 2.712 ± 0.265
2.712SerArg: 2.712 ± 0.332
4.163SerSer: 4.163 ± 0.627
3.532SerThr: 3.532 ± 0.38
4.447SerVal: 4.447 ± 0.393
0.757SerTrp: 0.757 ± 0.166
2.744SerTyr: 2.744 ± 0.377
0.0SerXaa: 0.0 ± 0.0
Thr
4.573ThrAla: 4.573 ± 0.392
0.473ThrCys: 0.473 ± 0.137
4.51ThrAsp: 4.51 ± 0.378
3.595ThrGlu: 3.595 ± 0.31
2.365ThrPhe: 2.365 ± 0.266
5.298ThrGly: 5.298 ± 0.498
1.482ThrHis: 1.482 ± 0.193
4.258ThrIle: 4.258 ± 0.394
4.1ThrLys: 4.1 ± 0.396
5.109ThrLeu: 5.109 ± 0.347
1.262ThrMet: 1.262 ± 0.186
3.185ThrAsn: 3.185 ± 0.357
2.901ThrPro: 2.901 ± 0.331
1.987ThrGln: 1.987 ± 0.232
2.712ThrArg: 2.712 ± 0.263
4.51ThrSer: 4.51 ± 0.509
3.879ThrThr: 3.879 ± 0.446
4.258ThrVal: 4.258 ± 0.436
0.725ThrTrp: 0.725 ± 0.165
2.302ThrTyr: 2.302 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
4.636ValAla: 4.636 ± 0.483
1.009ValCys: 1.009 ± 0.192
4.195ValAsp: 4.195 ± 0.4
4.573ValGlu: 4.573 ± 0.392
2.901ValPhe: 2.901 ± 0.307
4.131ValGly: 4.131 ± 0.436
1.672ValHis: 1.672 ± 0.228
4.605ValIle: 4.605 ± 0.359
5.141ValLys: 5.141 ± 0.4
4.888ValLeu: 4.888 ± 0.38
1.577ValMet: 1.577 ± 0.21
3.343ValAsn: 3.343 ± 0.384
1.987ValPro: 1.987 ± 0.323
2.397ValGln: 2.397 ± 0.294
2.428ValArg: 2.428 ± 0.286
4.541ValSer: 4.541 ± 0.46
4.352ValThr: 4.352 ± 0.417
5.046ValVal: 5.046 ± 0.48
1.009ValTrp: 1.009 ± 0.201
2.145ValTyr: 2.145 ± 0.252
0.0ValXaa: 0.0 ± 0.0
Trp
0.915TrpAla: 0.915 ± 0.165
0.221TrpCys: 0.221 ± 0.077
0.946TrpAsp: 0.946 ± 0.187
0.82TrpGlu: 0.82 ± 0.179
0.505TrpPhe: 0.505 ± 0.121
0.568TrpGly: 0.568 ± 0.134
0.378TrpHis: 0.378 ± 0.122
1.041TrpIle: 1.041 ± 0.172
0.883TrpLys: 0.883 ± 0.151
0.473TrpLeu: 0.473 ± 0.138
0.662TrpMet: 0.662 ± 0.159
0.946TrpAsn: 0.946 ± 0.184
0.0TrpPro: 0.0 ± 0.0
0.505TrpGln: 0.505 ± 0.127
0.536TrpArg: 0.536 ± 0.161
0.662TrpSer: 0.662 ± 0.132
0.757TrpThr: 0.757 ± 0.159
0.883TrpVal: 0.883 ± 0.178
0.189TrpTrp: 0.189 ± 0.083
0.378TrpTyr: 0.378 ± 0.103
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.807TyrAla: 2.807 ± 0.371
0.852TyrCys: 0.852 ± 0.167
2.491TyrAsp: 2.491 ± 0.252
1.735TyrGlu: 1.735 ± 0.224
1.577TyrPhe: 1.577 ± 0.229
2.334TyrGly: 2.334 ± 0.264
1.041TyrHis: 1.041 ± 0.194
2.334TyrIle: 2.334 ± 0.265
2.618TyrLys: 2.618 ± 0.286
2.838TyrLeu: 2.838 ± 0.347
0.915TyrMet: 0.915 ± 0.182
2.145TyrAsn: 2.145 ± 0.319
1.262TyrPro: 1.262 ± 0.184
1.23TyrGln: 1.23 ± 0.191
1.482TyrArg: 1.482 ± 0.192
2.555TyrSer: 2.555 ± 0.25
2.365TyrThr: 2.365 ± 0.276
2.239TyrVal: 2.239 ± 0.261
0.505TyrTrp: 0.505 ± 0.114
1.545TyrTyr: 1.545 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 149 proteins (31709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski