Amino acid dipepetide frequency for Tribolium castaneum (Red flour beetle)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.528AlaAla: 4.528 ± 0.041	1.113AlaCys: 1.113 ± 0.032	2.689AlaAsp: 2.689 ± 0.019	3.707AlaGlu: 3.707 ± 0.042	2.372AlaPhe: 2.372 ± 0.023	3.041AlaGly: 3.041 ± 0.027	1.285AlaHis: 1.285 ± 0.014	3.395AlaIle: 3.395 ± 0.025	3.941AlaLys: 3.941 ± 0.032	5.665AlaLeu: 5.665 ± 0.038	1.299AlaMet: 1.299 ± 0.015	2.673AlaAsn: 2.673 ± 0.022	3.037AlaPro: 3.037 ± 0.031	2.387AlaGln: 2.387 ± 0.022	2.899AlaArg: 2.899 ± 0.029	4.322AlaSer: 4.322 ± 0.029	3.584AlaThr: 3.584 ± 0.022	3.994AlaVal: 3.994 ± 0.024	0.579AlaTrp: 0.579 ± 0.013	1.543AlaTyr: 1.543 ± 0.017	0.0AlaXaa: 0.0 ± 0.0
Cys	1.136CysAla: 1.136 ± 0.019	0.514CysCys: 0.514 ± 0.01	1.285CysAsp: 1.285 ± 0.019	1.275CysGlu: 1.275 ± 0.019	0.856CysPhe: 0.856 ± 0.013	1.424CysGly: 1.424 ± 0.047	0.525CysHis: 0.525 ± 0.009	1.14CysIle: 1.14 ± 0.034	1.303CysLys: 1.303 ± 0.021	1.949CysLeu: 1.949 ± 0.027	0.39CysMet: 0.39 ± 0.006	1.017CysAsn: 1.017 ± 0.02	1.145CysPro: 1.145 ± 0.045	0.831CysGln: 0.831 ± 0.025	1.122CysArg: 1.122 ± 0.049	1.579CysSer: 1.579 ± 0.039	1.121CysThr: 1.121 ± 0.026	1.439CysVal: 1.439 ± 0.038	0.229CysTrp: 0.229 ± 0.005	0.678CysTyr: 0.678 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	2.72AspAla: 2.72 ± 0.018	1.088AspCys: 1.088 ± 0.02	3.578AspAsp: 3.578 ± 0.033	4.217AspGlu: 4.217 ± 0.033	2.472AspPhe: 2.472 ± 0.025	2.916AspGly: 2.916 ± 0.027	1.122AspHis: 1.122 ± 0.015	3.223AspIle: 3.223 ± 0.022	3.551AspLys: 3.551 ± 0.042	5.146AspLeu: 5.146 ± 0.034	1.103AspMet: 1.103 ± 0.011	2.663AspAsn: 2.663 ± 0.023	2.474AspPro: 2.474 ± 0.047	1.712AspGln: 1.712 ± 0.016	2.368AspArg: 2.368 ± 0.023	4.119AspSer: 4.119 ± 0.032	2.598AspThr: 2.598 ± 0.018	3.505AspVal: 3.505 ± 0.026	0.635AspTrp: 0.635 ± 0.011	1.906AspTyr: 1.906 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	4.033GluAla: 4.033 ± 0.042	1.306GluCys: 1.306 ± 0.05	3.989GluAsp: 3.989 ± 0.037	6.745GluGlu: 6.745 ± 0.144	2.394GluPhe: 2.394 ± 0.017	3.103GluGly: 3.103 ± 0.037	1.424GluHis: 1.424 ± 0.018	4.488GluIle: 4.488 ± 0.059	5.84GluLys: 5.84 ± 0.101	5.785GluLeu: 5.785 ± 0.04	1.573GluMet: 1.573 ± 0.017	3.96GluAsn: 3.96 ± 0.033	2.763GluPro: 2.763 ± 0.038	2.609GluGln: 2.609 ± 0.028	3.528GluArg: 3.528 ± 0.029	4.401GluSer: 4.401 ± 0.036	4.058GluThr: 4.058 ± 0.054	4.186GluVal: 4.186 ± 0.052	0.65GluTrp: 0.65 ± 0.011	1.904GluTyr: 1.904 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.345PheAla: 2.345 ± 0.021	0.954PheCys: 0.954 ± 0.013	2.303PheAsp: 2.303 ± 0.022	2.519PheGlu: 2.519 ± 0.021	1.924PhePhe: 1.924 ± 0.026	2.545PheGly: 2.545 ± 0.027	0.986PheHis: 0.986 ± 0.012	2.437PheIle: 2.437 ± 0.023	2.562PheLys: 2.562 ± 0.019	4.175PheLeu: 4.175 ± 0.039	0.896PheMet: 0.896 ± 0.011	2.055PheAsn: 2.055 ± 0.019	1.786PhePro: 1.786 ± 0.015	1.584PheGln: 1.584 ± 0.016	2.034PheArg: 2.034 ± 0.018	3.127PheSer: 3.127 ± 0.025	2.436PheThr: 2.436 ± 0.018	2.846PheVal: 2.846 ± 0.025	0.524PheTrp: 0.524 ± 0.009	1.592PheTyr: 1.592 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	3.198GlyAla: 3.198 ± 0.03	1.056GlyCys: 1.056 ± 0.021	2.881GlyAsp: 2.881 ± 0.026	3.199GlyGlu: 3.199 ± 0.025	2.382GlyPhe: 2.382 ± 0.022	3.942GlyGly: 3.942 ± 0.061	1.398GlyHis: 1.398 ± 0.017	2.942GlyIle: 2.942 ± 0.023	3.526GlyLys: 3.526 ± 0.034	4.52GlyLeu: 4.52 ± 0.033	1.064GlyMet: 1.064 ± 0.013	2.492GlyAsn: 2.492 ± 0.026	2.448GlyPro: 2.448 ± 0.032	2.069GlyGln: 2.069 ± 0.025	2.815GlyArg: 2.815 ± 0.025	4.101GlySer: 4.101 ± 0.033	2.969GlyThr: 2.969 ± 0.026	3.443GlyVal: 3.443 ± 0.025	0.671GlyTrp: 0.671 ± 0.013	1.975GlyTyr: 1.975 ± 0.021	0.001GlyXaa: 0.001 ± 0.0
His	1.166HisAla: 1.166 ± 0.016	0.581HisCys: 0.581 ± 0.011	1.073HisAsp: 1.073 ± 0.013	1.328HisGlu: 1.328 ± 0.015	1.123HisPhe: 1.123 ± 0.014	1.242HisGly: 1.242 ± 0.015	0.878HisHis: 0.878 ± 0.016	1.382HisIle: 1.382 ± 0.013	1.517HisLys: 1.517 ± 0.016	2.483HisLeu: 2.483 ± 0.022	0.556HisMet: 0.556 ± 0.01	1.176HisAsn: 1.176 ± 0.013	1.245HisPro: 1.245 ± 0.016	1.044HisGln: 1.044 ± 0.013	1.365HisArg: 1.365 ± 0.018	1.804HisSer: 1.804 ± 0.02	1.298HisThr: 1.298 ± 0.014	1.505HisVal: 1.505 ± 0.017	0.282HisTrp: 0.282 ± 0.006	0.854HisTyr: 0.854 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.378IleAla: 3.378 ± 0.022	1.299IleCys: 1.299 ± 0.025	3.135IleAsp: 3.135 ± 0.024	3.779IleGlu: 3.779 ± 0.04	2.668IlePhe: 2.668 ± 0.032	2.728IleGly: 2.728 ± 0.023	1.333IleHis: 1.333 ± 0.015	3.578IleIle: 3.578 ± 0.034	4.057IleLys: 4.057 ± 0.037	5.593IleLeu: 5.593 ± 0.043	1.148IleMet: 1.148 ± 0.014	3.018IleAsn: 3.018 ± 0.031	2.946IlePro: 2.946 ± 0.027	2.306IleGln: 2.306 ± 0.018	2.806IleArg: 2.806 ± 0.02	4.341IleSer: 4.341 ± 0.028	3.584IleThr: 3.584 ± 0.032	3.738IleVal: 3.738 ± 0.032	0.589IleTrp: 0.589 ± 0.011	1.952IleTyr: 1.952 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.713LysAla: 3.713 ± 0.035	1.556LysCys: 1.556 ± 0.031	3.478LysAsp: 3.478 ± 0.037	5.142LysGlu: 5.142 ± 0.074	2.648LysPhe: 2.648 ± 0.023	2.941LysGly: 2.941 ± 0.023	1.674LysHis: 1.674 ± 0.019	4.593LysIle: 4.593 ± 0.035	6.264LysLys: 6.264 ± 0.098	6.317LysLeu: 6.317 ± 0.037	1.571LysMet: 1.571 ± 0.015	3.89LysAsn: 3.89 ± 0.034	3.712LysPro: 3.712 ± 0.081	2.874LysGln: 2.874 ± 0.027	3.87LysArg: 3.87 ± 0.031	5.114LysSer: 5.114 ± 0.049	4.259LysThr: 4.259 ± 0.039	4.073LysVal: 4.073 ± 0.041	0.763LysTrp: 0.763 ± 0.012	2.341LysTyr: 2.341 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	5.707LeuAla: 5.707 ± 0.036	1.862LeuCys: 1.862 ± 0.02	4.85LeuAsp: 4.85 ± 0.031	6.324LeuGlu: 6.324 ± 0.04	3.7LeuPhe: 3.7 ± 0.038	4.72LeuGly: 4.72 ± 0.043	2.324LeuHis: 2.324 ± 0.023	5.065LeuIle: 5.065 ± 0.045	6.839LeuLys: 6.839 ± 0.043	8.991LeuLeu: 8.991 ± 0.071	1.939LeuMet: 1.939 ± 0.019	4.642LeuAsn: 4.642 ± 0.032	4.686LeuPro: 4.686 ± 0.038	4.489LeuGln: 4.489 ± 0.033	4.929LeuArg: 4.929 ± 0.034	6.855LeuSer: 6.855 ± 0.042	5.242LeuThr: 5.242 ± 0.031	5.563LeuVal: 5.563 ± 0.04	0.939LeuTrp: 0.939 ± 0.014	2.759LeuTyr: 2.759 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	1.5MetAla: 1.5 ± 0.019	0.435MetCys: 0.435 ± 0.008	1.139MetAsp: 1.139 ± 0.013	1.419MetGlu: 1.419 ± 0.015	0.912MetPhe: 0.912 ± 0.014	1.173MetGly: 1.173 ± 0.012	0.458MetHis: 0.458 ± 0.008	1.061MetIle: 1.061 ± 0.012	1.495MetLys: 1.495 ± 0.016	1.831MetLeu: 1.831 ± 0.018	0.556MetMet: 0.556 ± 0.01	0.989MetAsn: 0.989 ± 0.011	0.947MetPro: 0.947 ± 0.014	0.89MetGln: 0.89 ± 0.013	1.063MetArg: 1.063 ± 0.014	1.697MetSer: 1.697 ± 0.017	1.192MetThr: 1.192 ± 0.012	1.275MetVal: 1.275 ± 0.014	0.234MetTrp: 0.234 ± 0.006	0.651MetTyr: 0.651 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.726AsnAla: 2.726 ± 0.032	1.148AsnCys: 1.148 ± 0.021	2.514AsnAsp: 2.514 ± 0.027	3.331AsnGlu: 3.331 ± 0.023	2.349AsnPhe: 2.349 ± 0.024	2.846AsnGly: 2.846 ± 0.03	1.184AsnHis: 1.184 ± 0.024	3.106AsnIle: 3.106 ± 0.027	3.431AsnLys: 3.431 ± 0.03	4.954AsnLeu: 4.954 ± 0.034	1.097AsnMet: 1.097 ± 0.013	3.11AsnAsn: 3.11 ± 0.034	2.5AsnPro: 2.5 ± 0.045	2.029AsnGln: 2.029 ± 0.019	2.406AsnArg: 2.406 ± 0.021	3.972AsnSer: 3.972 ± 0.032	2.703AsnThr: 2.703 ± 0.021	3.346AsnVal: 3.346 ± 0.024	0.552AsnTrp: 0.552 ± 0.008	1.906AsnTyr: 1.906 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	2.807ProAla: 2.807 ± 0.027	0.909ProCys: 0.909 ± 0.067	2.708ProAsp: 2.708 ± 0.021	3.903ProGlu: 3.903 ± 0.083	1.879ProPhe: 1.879 ± 0.021	2.792ProGly: 2.792 ± 0.053	1.192ProHis: 1.192 ± 0.014	2.678ProIle: 2.678 ± 0.03	3.62ProLys: 3.62 ± 0.044	4.235ProLeu: 4.235 ± 0.027	0.902ProMet: 0.902 ± 0.015	2.539ProAsn: 2.539 ± 0.034	4.606ProPro: 4.606 ± 0.072	2.379ProGln: 2.379 ± 0.029	2.444ProArg: 2.444 ± 0.022	4.216ProSer: 4.216 ± 0.057	3.176ProThr: 3.176 ± 0.028	3.529ProVal: 3.529 ± 0.037	0.501ProTrp: 0.501 ± 0.008	1.551ProTyr: 1.551 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	2.362GlnAla: 2.362 ± 0.02	0.884GlnCys: 0.884 ± 0.029	1.834GlnAsp: 1.834 ± 0.016	2.857GlnGlu: 2.857 ± 0.032	1.658GlnPhe: 1.658 ± 0.016	1.915GlnGly: 1.915 ± 0.02	1.089GlnHis: 1.089 ± 0.013	2.545GlnIle: 2.545 ± 0.018	2.909GlnLys: 2.909 ± 0.025	3.892GlnLeu: 3.892 ± 0.032	0.968GlnMet: 0.968 ± 0.011	2.359GlnAsn: 2.359 ± 0.02	2.094GlnPro: 2.094 ± 0.024	2.554GlnGln: 2.554 ± 0.049	2.262GlnArg: 2.262 ± 0.031	2.719GlnSer: 2.719 ± 0.021	2.365GlnThr: 2.365 ± 0.019	2.534GlnVal: 2.534 ± 0.019	0.47GlnTrp: 0.47 ± 0.008	1.28GlnTyr: 1.28 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	2.76ArgAla: 2.76 ± 0.022	1.04ArgCys: 1.04 ± 0.021	2.645ArgAsp: 2.645 ± 0.022	3.321ArgGlu: 3.321 ± 0.026	2.073ArgPhe: 2.073 ± 0.017	2.587ArgGly: 2.587 ± 0.026	1.446ArgHis: 1.446 ± 0.015	2.891ArgIle: 2.891 ± 0.022	4.153ArgLys: 4.153 ± 0.034	4.578ArgLeu: 4.578 ± 0.04	1.023ArgMet: 1.023 ± 0.013	2.768ArgAsn: 2.768 ± 0.021	2.62ArgPro: 2.62 ± 0.034	2.218ArgGln: 2.218 ± 0.023	3.872ArgArg: 3.872 ± 0.038	3.778ArgSer: 3.778 ± 0.032	2.647ArgThr: 2.647 ± 0.02	2.891ArgVal: 2.891 ± 0.022	0.584ArgTrp: 0.584 ± 0.01	1.59ArgTyr: 1.59 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	4.184SerAla: 4.184 ± 0.027	1.497SerCys: 1.497 ± 0.04	4.454SerAsp: 4.454 ± 0.033	5.025SerGlu: 5.025 ± 0.047	2.955SerPhe: 2.955 ± 0.025	4.503SerGly: 4.503 ± 0.032	1.693SerHis: 1.693 ± 0.015	3.765SerIle: 3.765 ± 0.024	4.727SerLys: 4.727 ± 0.039	6.863SerLeu: 6.863 ± 0.044	1.422SerMet: 1.422 ± 0.016	3.714SerAsn: 3.714 ± 0.028	4.576SerPro: 4.576 ± 0.066	3.03SerGln: 3.03 ± 0.024	3.833SerArg: 3.833 ± 0.034	7.317SerSer: 7.317 ± 0.063	4.553SerThr: 4.553 ± 0.037	4.84SerVal: 4.84 ± 0.029	0.799SerTrp: 0.799 ± 0.01	2.233SerTyr: 2.233 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	3.456ThrAla: 3.456 ± 0.026	1.296ThrCys: 1.296 ± 0.033	2.89ThrAsp: 2.89 ± 0.02	3.78ThrGlu: 3.78 ± 0.064	2.45ThrPhe: 2.45 ± 0.024	3.122ThrGly: 3.122 ± 0.027	1.285ThrHis: 1.285 ± 0.011	3.519ThrIle: 3.519 ± 0.029	3.801ThrLys: 3.801 ± 0.034	5.292ThrLeu: 5.292 ± 0.034	1.11ThrMet: 1.11 ± 0.012	2.861ThrAsn: 2.861 ± 0.022	3.647ThrPro: 3.647 ± 0.037	2.223ThrGln: 2.223 ± 0.024	2.589ThrArg: 2.589 ± 0.023	4.754ThrSer: 4.754 ± 0.031	4.186ThrThr: 4.186 ± 0.052	4.006ThrVal: 4.006 ± 0.051	0.61ThrTrp: 0.61 ± 0.01	1.726ThrTyr: 1.726 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	4.108ValAla: 4.108 ± 0.029	1.455ValCys: 1.455 ± 0.026	3.316ValAsp: 3.316 ± 0.026	4.312ValGlu: 4.312 ± 0.065	2.716ValPhe: 2.716 ± 0.025	3.1ValGly: 3.1 ± 0.023	1.425ValHis: 1.425 ± 0.016	3.757ValIle: 3.757 ± 0.023	4.359ValLys: 4.359 ± 0.034	6.067ValLeu: 6.067 ± 0.044	1.326ValMet: 1.326 ± 0.014	2.958ValAsn: 2.958 ± 0.027	3.447ValPro: 3.447 ± 0.043	2.561ValGln: 2.561 ± 0.021	3.021ValArg: 3.021 ± 0.023	4.612ValSer: 4.612 ± 0.027	4.158ValThr: 4.158 ± 0.056	4.618ValVal: 4.618 ± 0.029	0.696ValTrp: 0.696 ± 0.01	1.944ValTyr: 1.944 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	0.571TrpAla: 0.571 ± 0.01	0.233TrpCys: 0.233 ± 0.005	0.585TrpAsp: 0.585 ± 0.01	0.618TrpGlu: 0.618 ± 0.012	0.484TrpPhe: 0.484 ± 0.008	0.609TrpGly: 0.609 ± 0.011	0.256TrpHis: 0.256 ± 0.006	0.637TrpIle: 0.637 ± 0.011	0.797TrpLys: 0.797 ± 0.012	1.057TrpLeu: 1.057 ± 0.015	0.277TrpMet: 0.277 ± 0.007	0.581TrpAsn: 0.581 ± 0.01	0.44TrpPro: 0.44 ± 0.009	0.439TrpGln: 0.439 ± 0.008	0.634TrpArg: 0.634 ± 0.01	0.812TrpSer: 0.812 ± 0.012	0.642TrpThr: 0.642 ± 0.01	0.63TrpVal: 0.63 ± 0.014	0.18TrpTrp: 0.18 ± 0.006	0.392TrpTyr: 0.392 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.654TyrAla: 1.654 ± 0.019	0.733TyrCys: 0.733 ± 0.011	1.771TyrAsp: 1.771 ± 0.017	1.938TyrGlu: 1.938 ± 0.018	1.619TyrPhe: 1.619 ± 0.02	1.843TyrGly: 1.843 ± 0.021	0.924TyrHis: 0.924 ± 0.017	1.772TyrIle: 1.772 ± 0.019	2.101TyrLys: 2.101 ± 0.023	3.031TyrLeu: 3.031 ± 0.027	0.696TyrMet: 0.696 ± 0.011	1.742TyrAsn: 1.742 ± 0.018	1.446TyrPro: 1.446 ± 0.018	1.325TyrGln: 1.325 ± 0.015	1.627TyrArg: 1.627 ± 0.013	2.346TyrSer: 2.346 ± 0.022	1.831TyrThr: 1.831 ± 0.022	1.994TyrVal: 1.994 ± 0.02	0.386TyrTrp: 0.386 ± 0.008	1.351TyrTyr: 1.351 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.029XaaXaa: 0.029 ± 0.009

Statistics based on 18505 proteins (9078672 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---