Amino acid dipepetide frequency for bacterium J17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.74AlaAla: 7.74 ± 0.285
0.884AlaCys: 0.884 ± 0.074
4.178AlaAsp: 4.178 ± 0.162
6.67AlaGlu: 6.67 ± 0.257
3.62AlaPhe: 3.62 ± 0.16
5.632AlaGly: 5.632 ± 0.204
1.333AlaHis: 1.333 ± 0.088
5.273AlaIle: 5.273 ± 0.187
5.497AlaLys: 5.497 ± 0.244
9.169AlaLeu: 9.169 ± 0.247
1.826AlaMet: 1.826 ± 0.105
3.28AlaAsn: 3.28 ± 0.133
2.781AlaPro: 2.781 ± 0.124
2.762AlaGln: 2.762 ± 0.137
4.312AlaArg: 4.312 ± 0.186
6.574AlaSer: 6.574 ± 0.184
3.575AlaThr: 3.575 ± 0.153
6.394AlaVal: 6.394 ± 0.221
0.737AlaTrp: 0.737 ± 0.066
2.031AlaTyr: 2.031 ± 0.129
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.073
0.147CysCys: 0.147 ± 0.03
0.545CysAsp: 0.545 ± 0.067
0.679CysGlu: 0.679 ± 0.071
0.525CysPhe: 0.525 ± 0.055
0.756CysGly: 0.756 ± 0.084
0.263CysHis: 0.263 ± 0.039
0.532CysIle: 0.532 ± 0.073
0.449CysLys: 0.449 ± 0.051
1.224CysLeu: 1.224 ± 0.101
0.231CysMet: 0.231 ± 0.04
0.288CysAsn: 0.288 ± 0.039
0.468CysPro: 0.468 ± 0.058
0.25CysGln: 0.25 ± 0.038
0.538CysArg: 0.538 ± 0.055
1.057CysSer: 1.057 ± 0.09
0.365CysThr: 0.365 ± 0.06
0.622CysVal: 0.622 ± 0.072
0.064CysTrp: 0.064 ± 0.019
0.333CysTyr: 0.333 ± 0.044
0.0CysXaa: 0.0 ± 0.0
Asp
3.985AspAla: 3.985 ± 0.159
0.602AspCys: 0.602 ± 0.074
2.941AspAsp: 2.941 ± 0.164
3.985AspGlu: 3.985 ± 0.179
2.858AspPhe: 2.858 ± 0.139
4.472AspGly: 4.472 ± 0.224
0.801AspHis: 0.801 ± 0.066
3.204AspIle: 3.204 ± 0.145
2.678AspLys: 2.678 ± 0.137
5.606AspLeu: 5.606 ± 0.201
0.756AspMet: 0.756 ± 0.079
1.845AspAsn: 1.845 ± 0.116
2.371AspPro: 2.371 ± 0.123
1.64AspGln: 1.64 ± 0.104
2.742AspArg: 2.742 ± 0.152
4.588AspSer: 4.588 ± 0.189
2.377AspThr: 2.377 ± 0.116
3.793AspVal: 3.793 ± 0.161
0.679AspTrp: 0.679 ± 0.072
1.73AspTyr: 1.73 ± 0.123
0.0AspXaa: 0.0 ± 0.0
Glu
5.709GluAla: 5.709 ± 0.23
0.391GluCys: 0.391 ± 0.052
3.716GluAsp: 3.716 ± 0.172
6.023GluGlu: 6.023 ± 0.305
2.915GluPhe: 2.915 ± 0.177
4.248GluGly: 4.248 ± 0.169
1.39GluHis: 1.39 ± 0.106
5.504GluIle: 5.504 ± 0.206
4.786GluLys: 4.786 ± 0.214
8.285GluLeu: 8.285 ± 0.257
1.493GluMet: 1.493 ± 0.096
3.056GluAsn: 3.056 ± 0.143
2.275GluPro: 2.275 ± 0.135
3.127GluGln: 3.127 ± 0.165
4.152GluArg: 4.152 ± 0.182
5.28GluSer: 5.28 ± 0.194
3.428GluThr: 3.428 ± 0.175
5.382GluVal: 5.382 ± 0.235
0.442GluTrp: 0.442 ± 0.057
1.512GluTyr: 1.512 ± 0.099
0.0GluXaa: 0.0 ± 0.0
Phe
4.113PheAla: 4.113 ± 0.166
0.577PheCys: 0.577 ± 0.059
2.64PheAsp: 2.64 ± 0.154
2.986PheGlu: 2.986 ± 0.141
2.46PhePhe: 2.46 ± 0.171
3.389PheGly: 3.389 ± 0.152
0.756PheHis: 0.756 ± 0.071
2.332PheIle: 2.332 ± 0.118
1.884PheLys: 1.884 ± 0.104
5.177PheLeu: 5.177 ± 0.205
0.666PheMet: 0.666 ± 0.069
1.39PheAsn: 1.39 ± 0.105
1.762PhePro: 1.762 ± 0.098
1.346PheGln: 1.346 ± 0.087
2.243PheArg: 2.243 ± 0.133
4.344PheSer: 4.344 ± 0.203
1.993PheThr: 1.993 ± 0.111
3.537PheVal: 3.537 ± 0.176
0.436PheTrp: 0.436 ± 0.058
1.474PheTyr: 1.474 ± 0.109
0.0PheXaa: 0.0 ± 0.0
Gly
5.811GlyAla: 5.811 ± 0.233
0.788GlyCys: 0.788 ± 0.083
4.075GlyAsp: 4.075 ± 0.167
5.324GlyGlu: 5.324 ± 0.185
3.441GlyPhe: 3.441 ± 0.145
5.574GlyGly: 5.574 ± 0.264
1.205GlyHis: 1.205 ± 0.092
4.184GlyIle: 4.184 ± 0.208
4.242GlyLys: 4.242 ± 0.187
7.227GlyLeu: 7.227 ± 0.229
1.48GlyMet: 1.48 ± 0.095
2.351GlyAsn: 2.351 ± 0.166
1.961GlyPro: 1.961 ± 0.115
2.127GlyGln: 2.127 ± 0.121
3.71GlyArg: 3.71 ± 0.15
5.581GlySer: 5.581 ± 0.219
3.511GlyThr: 3.511 ± 0.197
5.702GlyVal: 5.702 ± 0.22
0.647GlyTrp: 0.647 ± 0.067
1.999GlyTyr: 1.999 ± 0.103
0.0GlyXaa: 0.0 ± 0.0
His
1.192HisAla: 1.192 ± 0.079
0.231HisCys: 0.231 ± 0.033
0.794HisAsp: 0.794 ± 0.071
0.833HisGlu: 0.833 ± 0.076
1.006HisPhe: 1.006 ± 0.078
1.128HisGly: 1.128 ± 0.086
0.378HisHis: 0.378 ± 0.049
0.903HisIle: 0.903 ± 0.077
0.762HisLys: 0.762 ± 0.073
1.916HisLeu: 1.916 ± 0.119
0.276HisMet: 0.276 ± 0.046
0.634HisAsn: 0.634 ± 0.072
0.903HisPro: 0.903 ± 0.08
0.487HisGln: 0.487 ± 0.062
1.121HisArg: 1.121 ± 0.091
1.461HisSer: 1.461 ± 0.104
0.692HisThr: 0.692 ± 0.078
1.128HisVal: 1.128 ± 0.087
0.231HisTrp: 0.231 ± 0.04
0.66HisTyr: 0.66 ± 0.065
0.0HisXaa: 0.0 ± 0.0
Ile
6.106IleAla: 6.106 ± 0.184
0.833IleCys: 0.833 ± 0.073
4.126IleAsp: 4.126 ± 0.169
4.581IleGlu: 4.581 ± 0.172
2.403IlePhe: 2.403 ± 0.112
4.165IleGly: 4.165 ± 0.224
0.935IleHis: 0.935 ± 0.089
3.505IleIle: 3.505 ± 0.164
2.819IleLys: 2.819 ± 0.156
5.465IleLeu: 5.465 ± 0.192
0.788IleMet: 0.788 ± 0.074
2.268IleAsn: 2.268 ± 0.121
2.736IlePro: 2.736 ± 0.164
1.653IleGln: 1.653 ± 0.099
2.864IleArg: 2.864 ± 0.137
5.401IleSer: 5.401 ± 0.173
2.851IleThr: 2.851 ± 0.138
4.581IleVal: 4.581 ± 0.173
0.551IleTrp: 0.551 ± 0.073
1.595IleTyr: 1.595 ± 0.11
0.0IleXaa: 0.0 ± 0.0
Lys
4.338LysAla: 4.338 ± 0.193
0.295LysCys: 0.295 ± 0.05
2.928LysAsp: 2.928 ± 0.152
4.594LysGlu: 4.594 ± 0.22
2.05LysPhe: 2.05 ± 0.113
3.146LysGly: 3.146 ± 0.171
0.942LysHis: 0.942 ± 0.08
3.921LysIle: 3.921 ± 0.172
3.729LysLys: 3.729 ± 0.155
5.715LysLeu: 5.715 ± 0.223
1.0LysMet: 1.0 ± 0.083
2.403LysAsn: 2.403 ± 0.127
2.377LysPro: 2.377 ± 0.149
2.063LysGln: 2.063 ± 0.123
3.216LysArg: 3.216 ± 0.135
4.421LysSer: 4.421 ± 0.207
2.845LysThr: 2.845 ± 0.126
3.774LysVal: 3.774 ± 0.158
0.384LysTrp: 0.384 ± 0.053
1.461LysTyr: 1.461 ± 0.11
0.0LysXaa: 0.0 ± 0.0
Leu
9.707LeuAla: 9.707 ± 0.283
1.166LeuCys: 1.166 ± 0.085
5.69LeuAsp: 5.69 ± 0.222
7.58LeuGlu: 7.58 ± 0.241
4.677LeuPhe: 4.677 ± 0.236
8.003LeuGly: 8.003 ± 0.23
1.583LeuHis: 1.583 ± 0.113
5.722LeuIle: 5.722 ± 0.234
6.016LeuLys: 6.016 ± 0.245
12.372LeuLeu: 12.372 ± 0.404
2.076LeuMet: 2.076 ± 0.128
3.774LeuAsn: 3.774 ± 0.16
4.959LeuPro: 4.959 ± 0.177
3.165LeuGln: 3.165 ± 0.129
6.202LeuArg: 6.202 ± 0.227
9.694LeuSer: 9.694 ± 0.268
4.671LeuThr: 4.671 ± 0.17
8.073LeuVal: 8.073 ± 0.277
0.865LeuTrp: 0.865 ± 0.073
2.134LeuTyr: 2.134 ± 0.139
0.0LeuXaa: 0.0 ± 0.0
Met
1.57MetAla: 1.57 ± 0.093
0.147MetCys: 0.147 ± 0.029
0.782MetAsp: 0.782 ± 0.068
1.121MetGlu: 1.121 ± 0.086
0.807MetPhe: 0.807 ± 0.075
1.281MetGly: 1.281 ± 0.094
0.301MetHis: 0.301 ± 0.047
1.166MetIle: 1.166 ± 0.087
1.076MetLys: 1.076 ± 0.077
2.262MetLeu: 2.262 ± 0.13
0.449MetMet: 0.449 ± 0.062
0.622MetAsn: 0.622 ± 0.069
1.0MetPro: 1.0 ± 0.083
0.628MetGln: 0.628 ± 0.062
0.993MetArg: 0.993 ± 0.078
1.659MetSer: 1.659 ± 0.105
0.961MetThr: 0.961 ± 0.078
1.23MetVal: 1.23 ± 0.104
0.109MetTrp: 0.109 ± 0.03
0.416MetTyr: 0.416 ± 0.052
0.0MetXaa: 0.0 ± 0.0
Asn
2.665AsnAla: 2.665 ± 0.145
0.487AsnCys: 0.487 ± 0.056
1.845AsnAsp: 1.845 ± 0.105
2.217AsnGlu: 2.217 ± 0.119
1.647AsnPhe: 1.647 ± 0.099
2.326AsnGly: 2.326 ± 0.142
0.532AsnHis: 0.532 ± 0.053
2.633AsnIle: 2.633 ± 0.135
1.711AsnLys: 1.711 ± 0.108
3.979AsnLeu: 3.979 ± 0.153
0.577AsnMet: 0.577 ± 0.069
1.672AsnAsn: 1.672 ± 0.121
2.082AsnPro: 2.082 ± 0.108
1.41AsnGln: 1.41 ± 0.091
1.852AsnArg: 1.852 ± 0.122
3.3AsnSer: 3.3 ± 0.167
1.743AsnThr: 1.743 ± 0.119
2.217AsnVal: 2.217 ± 0.129
0.525AsnTrp: 0.525 ± 0.062
1.147AsnTyr: 1.147 ± 0.088
0.0AsnXaa: 0.0 ± 0.0
Pro
3.434ProAla: 3.434 ± 0.175
0.359ProCys: 0.359 ± 0.045
2.095ProAsp: 2.095 ± 0.124
3.588ProGlu: 3.588 ± 0.144
1.775ProPhe: 1.775 ± 0.11
3.114ProGly: 3.114 ± 0.144
0.73ProHis: 0.73 ± 0.077
2.351ProIle: 2.351 ± 0.134
2.518ProLys: 2.518 ± 0.136
3.928ProLeu: 3.928 ± 0.181
0.794ProMet: 0.794 ± 0.074
1.672ProAsn: 1.672 ± 0.099
1.397ProPro: 1.397 ± 0.116
1.262ProGln: 1.262 ± 0.095
1.781ProArg: 1.781 ± 0.106
3.05ProSer: 3.05 ± 0.135
1.973ProThr: 1.973 ± 0.119
3.255ProVal: 3.255 ± 0.165
0.532ProTrp: 0.532 ± 0.057
1.0ProTyr: 1.0 ± 0.076
0.0ProXaa: 0.0 ± 0.0
Gln
2.902GlnAla: 2.902 ± 0.15
0.295GlnCys: 0.295 ± 0.048
1.467GlnAsp: 1.467 ± 0.105
2.319GlnGlu: 2.319 ± 0.124
1.41GlnPhe: 1.41 ± 0.085
2.243GlnGly: 2.243 ± 0.112
0.545GlnHis: 0.545 ± 0.066
2.076GlnIle: 2.076 ± 0.13
1.858GlnLys: 1.858 ± 0.111
3.697GlnLeu: 3.697 ± 0.156
0.673GlnMet: 0.673 ± 0.074
1.096GlnAsn: 1.096 ± 0.09
1.288GlnPro: 1.288 ± 0.08
1.346GlnGln: 1.346 ± 0.115
1.986GlnArg: 1.986 ± 0.124
2.678GlnSer: 2.678 ± 0.155
1.621GlnThr: 1.621 ± 0.104
2.486GlnVal: 2.486 ± 0.132
0.327GlnTrp: 0.327 ± 0.054
0.679GlnTyr: 0.679 ± 0.07
0.0GlnXaa: 0.0 ± 0.0
Arg
4.761ArgAla: 4.761 ± 0.226
0.519ArgCys: 0.519 ± 0.06
2.8ArgAsp: 2.8 ± 0.173
4.12ArgGlu: 4.12 ± 0.161
2.383ArgPhe: 2.383 ± 0.118
3.441ArgGly: 3.441 ± 0.142
0.993ArgHis: 0.993 ± 0.075
3.3ArgIle: 3.3 ± 0.152
3.088ArgLys: 3.088 ± 0.16
5.632ArgLeu: 5.632 ± 0.19
1.205ArgMet: 1.205 ± 0.091
1.948ArgAsn: 1.948 ± 0.126
2.23ArgPro: 2.23 ± 0.127
1.685ArgGln: 1.685 ± 0.099
3.46ArgArg: 3.46 ± 0.174
3.992ArgSer: 3.992 ± 0.156
2.416ArgThr: 2.416 ± 0.121
4.126ArgVal: 4.126 ± 0.164
0.596ArgTrp: 0.596 ± 0.067
1.589ArgTyr: 1.589 ± 0.094
0.0ArgXaa: 0.0 ± 0.0
Ser
6.798SerAla: 6.798 ± 0.207
0.718SerCys: 0.718 ± 0.08
4.504SerAsp: 4.504 ± 0.157
5.952SerGlu: 5.952 ± 0.221
4.126SerPhe: 4.126 ± 0.183
6.189SerGly: 6.189 ± 0.206
1.269SerHis: 1.269 ± 0.096
4.607SerIle: 4.607 ± 0.183
4.632SerLys: 4.632 ± 0.197
9.111SerLeu: 9.111 ± 0.288
1.563SerMet: 1.563 ± 0.112
2.838SerAsn: 2.838 ± 0.14
3.345SerPro: 3.345 ± 0.145
2.915SerGln: 2.915 ± 0.155
4.312SerArg: 4.312 ± 0.152
7.868SerSer: 7.868 ± 0.272
3.832SerThr: 3.832 ± 0.171
5.888SerVal: 5.888 ± 0.216
0.794SerTrp: 0.794 ± 0.074
2.409SerTyr: 2.409 ± 0.137
0.0SerXaa: 0.0 ± 0.0
Thr
4.005ThrAla: 4.005 ± 0.146
0.365ThrCys: 0.365 ± 0.043
2.275ThrAsp: 2.275 ± 0.124
3.05ThrGlu: 3.05 ± 0.155
2.076ThrPhe: 2.076 ± 0.123
3.421ThrGly: 3.421 ± 0.161
0.788ThrHis: 0.788 ± 0.072
3.018ThrIle: 3.018 ± 0.124
2.294ThrLys: 2.294 ± 0.118
4.914ThrLeu: 4.914 ± 0.16
0.846ThrMet: 0.846 ± 0.079
1.672ThrAsn: 1.672 ± 0.094
2.204ThrPro: 2.204 ± 0.143
1.294ThrGln: 1.294 ± 0.093
2.396ThrArg: 2.396 ± 0.115
3.729ThrSer: 3.729 ± 0.146
2.307ThrThr: 2.307 ± 0.141
3.447ThrVal: 3.447 ± 0.162
0.429ThrTrp: 0.429 ± 0.055
1.108ThrTyr: 1.108 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
6.273ValAla: 6.273 ± 0.234
0.871ValCys: 0.871 ± 0.075
4.376ValAsp: 4.376 ± 0.169
5.421ValGlu: 5.421 ± 0.222
3.357ValPhe: 3.357 ± 0.169
5.811ValGly: 5.811 ± 0.248
1.224ValHis: 1.224 ± 0.1
4.21ValIle: 4.21 ± 0.173
3.735ValLys: 3.735 ± 0.147
7.804ValLeu: 7.804 ± 0.275
1.326ValMet: 1.326 ± 0.101
2.531ValAsn: 2.531 ± 0.14
2.973ValPro: 2.973 ± 0.165
2.121ValGln: 2.121 ± 0.114
3.908ValArg: 3.908 ± 0.173
6.183ValSer: 6.183 ± 0.23
3.133ValThr: 3.133 ± 0.169
6.196ValVal: 6.196 ± 0.261
0.666ValTrp: 0.666 ± 0.068
1.711ValTyr: 1.711 ± 0.115
0.0ValXaa: 0.0 ± 0.0
Trp
0.73TrpAla: 0.73 ± 0.067
0.077TrpCys: 0.077 ± 0.021
0.455TrpAsp: 0.455 ± 0.05
0.5TrpGlu: 0.5 ± 0.057
0.532TrpPhe: 0.532 ± 0.072
0.686TrpGly: 0.686 ± 0.068
0.211TrpHis: 0.211 ± 0.043
0.557TrpIle: 0.557 ± 0.059
0.455TrpLys: 0.455 ± 0.056
1.237TrpLeu: 1.237 ± 0.104
0.167TrpMet: 0.167 ± 0.032
0.404TrpAsn: 0.404 ± 0.054
0.391TrpPro: 0.391 ± 0.051
0.352TrpGln: 0.352 ± 0.052
0.666TrpArg: 0.666 ± 0.073
0.762TrpSer: 0.762 ± 0.074
0.327TrpThr: 0.327 ± 0.043
0.596TrpVal: 0.596 ± 0.068
0.16TrpTrp: 0.16 ± 0.03
0.218TrpTyr: 0.218 ± 0.035
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.698TyrAla: 1.698 ± 0.108
0.314TyrCys: 0.314 ± 0.049
1.422TyrAsp: 1.422 ± 0.106
1.57TyrGlu: 1.57 ± 0.101
1.435TyrPhe: 1.435 ± 0.107
1.916TyrGly: 1.916 ± 0.108
0.557TyrHis: 0.557 ± 0.059
1.23TyrIle: 1.23 ± 0.095
1.371TyrLys: 1.371 ± 0.1
3.409TyrLeu: 3.409 ± 0.158
0.384TyrMet: 0.384 ± 0.049
0.82TyrAsn: 0.82 ± 0.069
1.108TyrPro: 1.108 ± 0.09
1.358TyrGln: 1.358 ± 0.087
1.832TyrArg: 1.832 ± 0.104
2.05TyrSer: 2.05 ± 0.14
1.019TyrThr: 1.019 ± 0.081
1.403TyrVal: 1.403 ± 0.104
0.308TyrTrp: 0.308 ± 0.047
0.903TyrTyr: 0.903 ± 0.088
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 527 proteins (156075 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski