Neurachne minor latent virus
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1TXG5|A0A2S1TXG5_9VIRU Fusion protein OS=Neurachne minor latent virus OX=2175246 PE=4 SV=1
MM1 pKa = 7.57 PVDD4 pKa = 3.66 PLRR7 pKa = 11.84 TIEE10 pKa = 4.3 EE11 pKa = 4.11 TDD13 pKa = 3.29 EE14 pKa = 4.31 LATEE18 pKa = 4.25 EE19 pKa = 4.4 SNRR22 pKa = 11.84 IADD25 pKa = 3.9 LMNVFNEE32 pKa = 4.36 SALTRR37 pKa = 11.84 AQCTRR42 pKa = 11.84 NDD44 pKa = 4.11 IIACHH49 pKa = 4.6 QTVDD53 pKa = 4.36 EE54 pKa = 4.48 LDD56 pKa = 3.22 KK57 pKa = 11.11 CIRR60 pKa = 11.84 AVKK63 pKa = 10.21 SYY65 pKa = 10.93 VEE67 pKa = 3.9 QRR69 pKa = 11.84 TFDD72 pKa = 4.24 DD73 pKa = 4.2 LWTQAHH79 pKa = 6.12 NAGSVASAQVLTFRR93 pKa = 11.84 DD94 pKa = 3.49 LFRR97 pKa = 11.84 FKK99 pKa = 11.01 KK100 pKa = 10.53 FITSPAGQNVVRR112 pKa = 11.84 NVQAKK117 pKa = 10.13 KK118 pKa = 9.76 KK119 pKa = 8.77 LQRR122 pKa = 11.84 AGRR125 pKa = 11.84 TKK127 pKa = 10.76 HH128 pKa = 6.17 EE129 pKa = 4.41 ADD131 pKa = 3.2 QVALTRR137 pKa = 11.84 LFTTQYY143 pKa = 8.9 EE144 pKa = 4.29 VRR146 pKa = 11.84 ARR148 pKa = 11.84 GVTKK152 pKa = 10.72 DD153 pKa = 3.56 NGEE156 pKa = 4.27 LDD158 pKa = 3.11 IKK160 pKa = 10.18 AAKK163 pKa = 9.44 IRR165 pKa = 11.84 ARR167 pKa = 11.84 LEE169 pKa = 3.81 KK170 pKa = 10.24 INRR173 pKa = 11.84 KK174 pKa = 9.52 KK175 pKa = 10.83 EE176 pKa = 3.75 EE177 pKa = 4.7 NIQKK181 pKa = 9.64 WDD183 pKa = 3.54 DD184 pKa = 3.63 RR185 pKa = 11.84 CPVVAGYY192 pKa = 10.17 QEE194 pKa = 3.91 LTIAQVRR201 pKa = 11.84 EE202 pKa = 4.47 EE203 pKa = 3.93 AWKK206 pKa = 9.5 MYY208 pKa = 9.86 LQQCAAEE215 pKa = 4.64 HH216 pKa = 6.98 RR217 pKa = 11.84 DD218 pKa = 3.82 PPPKK222 pKa = 9.53 STRR225 pKa = 11.84 NLEE228 pKa = 3.91 AVAEE232 pKa = 4.21 KK233 pKa = 10.37 FGAEE237 pKa = 4.07 VKK239 pKa = 10.18 EE240 pKa = 4.1 DD241 pKa = 4.02 HH242 pKa = 6.77 LVQYY246 pKa = 7.38 CTNEE250 pKa = 3.78 RR251 pKa = 11.84 VRR253 pKa = 11.84 AALISFGEE261 pKa = 3.92 EE262 pKa = 3.63 HH263 pKa = 6.78 IKK265 pKa = 10.8 NLEE268 pKa = 3.98 KK269 pKa = 10.79 EE270 pKa = 4.25 GTRR273 pKa = 11.84 RR274 pKa = 11.84 NVEE277 pKa = 3.82 TFRR280 pKa = 11.84 NLVVIANKK288 pKa = 10.77 GEE290 pKa = 4.16 VPEE293 pKa = 4.71 IPAAGASPVDD303 pKa = 4.01 DD304 pKa = 3.86 EE305 pKa = 4.37 DD306 pKa = 3.73 TRR308 pKa = 11.84 RR309 pKa = 11.84 EE310 pKa = 4.03 DD311 pKa = 3.62 TPDD314 pKa = 3.46 PEE316 pKa = 4.29 YY317 pKa = 11.49 VEE319 pKa = 4.9 DD320 pKa = 3.94 QEE322 pKa = 4.3 TGRR325 pKa = 11.84 RR326 pKa = 11.84 DD327 pKa = 3.44 EE328 pKa = 4.39 EE329 pKa = 4.14 QSSEE333 pKa = 4.29 TEE335 pKa = 3.76 GDD337 pKa = 3.43 SGKK340 pKa = 10.39 KK341 pKa = 10.15 AEE343 pKa = 4.3 AAGTSRR349 pKa = 11.84 PRR351 pKa = 11.84 KK352 pKa = 9.08 RR353 pKa = 11.84 GRR355 pKa = 11.84 PTASTRR361 pKa = 11.84 RR362 pKa = 11.84 EE363 pKa = 3.97 RR364 pKa = 11.84 EE365 pKa = 3.69 AEE367 pKa = 3.79 AARR370 pKa = 11.84 EE371 pKa = 3.93 KK372 pKa = 11.06 GKK374 pKa = 10.63 SKK376 pKa = 10.43 KK377 pKa = 9.02 QRR379 pKa = 11.84 HH380 pKa = 5.89 SNDD383 pKa = 2.73
Molecular weight: 43.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.338
IPC2_protein 6.326
IPC_protein 6.427
Toseland 6.547
ProMoST 6.561
Dawson 6.766
Bjellqvist 6.707
Wikipedia 6.751
Rodwell 6.751
Grimsley 6.634
Solomon 6.781
Lehninger 6.766
Nozaki 7.0
DTASelect 7.146
Thurlkill 7.161
EMBOSS 7.161
Sillero 7.146
Patrickios 4.838
IPC_peptide 6.795
IPC2_peptide 6.81
IPC2.peptide.svr19 6.791
Protein with the highest isoelectric point:
>tr|A0A2S1TXG5|A0A2S1TXG5_9VIRU Fusion protein OS=Neurachne minor latent virus OX=2175246 PE=4 SV=1
MM1 pKa = 7.57 PVDD4 pKa = 3.66 PLRR7 pKa = 11.84 TIEE10 pKa = 4.3 EE11 pKa = 4.11 TDD13 pKa = 3.29 EE14 pKa = 4.31 LATEE18 pKa = 4.25 EE19 pKa = 4.4 SNRR22 pKa = 11.84 IADD25 pKa = 3.9 LMNVFNEE32 pKa = 4.36 SALTRR37 pKa = 11.84 AQCTRR42 pKa = 11.84 NDD44 pKa = 4.11 IIACHH49 pKa = 4.6 QTVDD53 pKa = 4.36 EE54 pKa = 4.48 LDD56 pKa = 3.22 KK57 pKa = 11.11 CIRR60 pKa = 11.84 AVKK63 pKa = 10.21 SYY65 pKa = 10.93 VEE67 pKa = 3.9 QRR69 pKa = 11.84 TFDD72 pKa = 4.24 DD73 pKa = 4.2 LWTQAHH79 pKa = 6.12 NAGSVASAQVLTFRR93 pKa = 11.84 DD94 pKa = 3.49 LFRR97 pKa = 11.84 FKK99 pKa = 11.01 KK100 pKa = 10.53 FITSPAGQNVVRR112 pKa = 11.84 NVQAKK117 pKa = 10.13 KK118 pKa = 9.76 KK119 pKa = 8.77 LQRR122 pKa = 11.84 AGRR125 pKa = 11.84 TKK127 pKa = 10.76 HH128 pKa = 6.17 EE129 pKa = 4.41 ADD131 pKa = 3.2 QVALTRR137 pKa = 11.84 LFTTQYY143 pKa = 8.9 EE144 pKa = 4.29 VRR146 pKa = 11.84 ARR148 pKa = 11.84 GVTKK152 pKa = 10.72 DD153 pKa = 3.56 NGEE156 pKa = 4.27 LDD158 pKa = 3.11 IKK160 pKa = 10.18 AAKK163 pKa = 9.44 IRR165 pKa = 11.84 ARR167 pKa = 11.84 LEE169 pKa = 3.81 KK170 pKa = 10.24 INRR173 pKa = 11.84 KK174 pKa = 9.52 KK175 pKa = 10.83 EE176 pKa = 3.75 EE177 pKa = 4.7 NIQKK181 pKa = 9.64 WDD183 pKa = 3.54 DD184 pKa = 3.63 RR185 pKa = 11.84 CPVVAGYY192 pKa = 10.17 QEE194 pKa = 3.91 LTIAQVRR201 pKa = 11.84 EE202 pKa = 4.47 EE203 pKa = 3.93 AWKK206 pKa = 9.5 MYY208 pKa = 9.86 LQQCAAEE215 pKa = 4.64 HH216 pKa = 6.98 RR217 pKa = 11.84 DD218 pKa = 3.82 PPPKK222 pKa = 9.53 STRR225 pKa = 11.84 NLEE228 pKa = 3.91 AVAEE232 pKa = 4.21 KK233 pKa = 10.37 FGAEE237 pKa = 4.07 VKK239 pKa = 10.18 EE240 pKa = 4.1 DD241 pKa = 4.02 HH242 pKa = 6.77 LVQYY246 pKa = 7.38 CTNEE250 pKa = 3.78 RR251 pKa = 11.84 VRR253 pKa = 11.84 AALISFGEE261 pKa = 3.92 EE262 pKa = 3.63 HH263 pKa = 6.78 IKK265 pKa = 10.8 NLEE268 pKa = 3.98 KK269 pKa = 10.79 EE270 pKa = 4.25 GTRR273 pKa = 11.84 RR274 pKa = 11.84 NVEE277 pKa = 4.01 TFVTSWSSQTKK288 pKa = 10.11 VKK290 pKa = 10.3 FLRR293 pKa = 11.84 YY294 pKa = 8.34 PLPEE298 pKa = 3.78 RR299 pKa = 11.84 RR300 pKa = 11.84 RR301 pKa = 11.84 LMTRR305 pKa = 11.84 IPVGKK310 pKa = 9.91 IPPTLSMLRR319 pKa = 11.84 TRR321 pKa = 11.84 RR322 pKa = 11.84 LADD325 pKa = 3.26 VMRR328 pKa = 11.84 SSLLRR333 pKa = 11.84 RR334 pKa = 11.84 KK335 pKa = 8.34 ATAGRR340 pKa = 11.84 RR341 pKa = 11.84 LKK343 pKa = 10.66 PLVPRR348 pKa = 11.84 GLEE351 pKa = 4.22 SVGDD355 pKa = 4.33 PLPLLAGNGRR365 pKa = 11.84 LKK367 pKa = 11.05 LLGRR371 pKa = 11.84 RR372 pKa = 11.84 GRR374 pKa = 11.84 VRR376 pKa = 11.84 NNAIQMTKK384 pKa = 8.7 SHH386 pKa = 6.52 WEE388 pKa = 3.53 AGVRR392 pKa = 11.84 HH393 pKa = 6.68 IIGGGEE399 pKa = 4.4 LINWRR404 pKa = 11.84 ADD406 pKa = 3.19 NNKK409 pKa = 9.39 YY410 pKa = 10.43 RR411 pKa = 11.84 GGGCLYY417 pKa = 10.81 DD418 pKa = 4.85 ALLLLANADD427 pKa = 3.79 DD428 pKa = 3.72 VTPFQEE434 pKa = 5.26 LGDD437 pKa = 3.8 HH438 pKa = 5.74 FQLRR442 pKa = 11.84 EE443 pKa = 3.74 ARR445 pKa = 11.84 DD446 pKa = 3.73 ILKK449 pKa = 10.28 LPCGLKK455 pKa = 10.53 VPDD458 pKa = 3.91 GRR460 pKa = 11.84 GACTMKK466 pKa = 10.4 HH467 pKa = 5.82 YY468 pKa = 11.11 NDD470 pKa = 5.02 DD471 pKa = 3.67 ATAGPLLRR479 pKa = 11.84 AFGIKK484 pKa = 9.78 GKK486 pKa = 10.58 YY487 pKa = 8.0 GLKK490 pKa = 9.43 TVVEE494 pKa = 4.32 DD495 pKa = 4.93 FVWEE499 pKa = 4.44 VYY501 pKa = 11.26 DD502 pKa = 3.52 MAGNGLLGIDD512 pKa = 4.03 QLPALLARR520 pKa = 11.84 VGYY523 pKa = 9.88 RR524 pKa = 11.84 SKK526 pKa = 11.18 LHH528 pKa = 6.68 DD529 pKa = 4.21 RR530 pKa = 11.84 PAAMKK535 pKa = 10.01 KK536 pKa = 9.92 ISEE539 pKa = 4.3 LKK541 pKa = 8.84 PLGRR545 pKa = 11.84 AVMMLDD551 pKa = 2.99 ATEE554 pKa = 4.14 QAFSSPIFNVISDD567 pKa = 4.19 IVTKK571 pKa = 10.9 LHH573 pKa = 7.27 DD574 pKa = 5.09 DD575 pKa = 3.72 KK576 pKa = 11.88 SSGWRR581 pKa = 11.84 NYY583 pKa = 9.56 LVRR586 pKa = 11.84 ASSGWIDD593 pKa = 3.18 LWDD596 pKa = 3.87 EE597 pKa = 4.12 VRR599 pKa = 11.84 DD600 pKa = 3.74 SQVIVEE606 pKa = 4.56 LDD608 pKa = 2.8 WSKK611 pKa = 10.98 FDD613 pKa = 4.72 RR614 pKa = 11.84 EE615 pKa = 4.24 RR616 pKa = 11.84 PRR618 pKa = 11.84 QDD620 pKa = 2.43 IQFFIDD626 pKa = 4.5 IICSCFSPSSLRR638 pKa = 11.84 EE639 pKa = 3.96 EE640 pKa = 4.55 LLLAAYY646 pKa = 9.62 KK647 pKa = 11.01 KK648 pKa = 10.09 MMEE651 pKa = 4.01 NALVNRR657 pKa = 11.84 VMLLDD662 pKa = 3.93 NGSIVTMDD670 pKa = 3.23 GMVPSGSLWTGICDD684 pKa = 3.33 TALNILYY691 pKa = 10.23 INAALIKK698 pKa = 10.53 LGFGRR703 pKa = 11.84 NSFNPKK709 pKa = 9.91 CAGDD713 pKa = 4.0 DD714 pKa = 3.86 NITLFTRR721 pKa = 11.84 RR722 pKa = 11.84 YY723 pKa = 7.77 STLEE727 pKa = 3.59 LTRR730 pKa = 11.84 LRR732 pKa = 11.84 DD733 pKa = 3.48 ALNEE737 pKa = 3.84 MFRR740 pKa = 11.84 AGIEE744 pKa = 3.7 EE745 pKa = 4.4 DD746 pKa = 3.67 EE747 pKa = 5.18 FIIHH751 pKa = 6.06 YY752 pKa = 9.84 PPFFVSRR759 pKa = 11.84 EE760 pKa = 3.93 QAQFPPNVDD769 pKa = 3.08 IEE771 pKa = 4.43 KK772 pKa = 8.82 GTSKK776 pKa = 10.6 IIDD779 pKa = 3.44 QAKK782 pKa = 9.11 WIPLTDD788 pKa = 3.59 EE789 pKa = 5.3 LIVDD793 pKa = 4.32 RR794 pKa = 11.84 FRR796 pKa = 11.84 GLSHH800 pKa = 6.71 RR801 pKa = 11.84 WRR803 pKa = 11.84 YY804 pKa = 9.05 NFHH807 pKa = 7.21 AKK809 pKa = 10.05 PKK811 pKa = 9.49 FLANFFLPDD820 pKa = 3.47 GRR822 pKa = 11.84 PIRR825 pKa = 11.84 PAADD829 pKa = 3.19 SLEE832 pKa = 4.0 RR833 pKa = 11.84 LLWPEE838 pKa = 4.33 GVHH841 pKa = 6.97 KK842 pKa = 11.07 SLDD845 pKa = 3.78 DD846 pKa = 3.92 YY847 pKa = 11.89 IMAVLAMIVDD857 pKa = 4.02 NPFNHH862 pKa = 6.7 HH863 pKa = 6.07 NVNHH867 pKa = 5.35 MMVRR871 pKa = 11.84 YY872 pKa = 9.97 IIAHH876 pKa = 5.08 QLRR879 pKa = 11.84 EE880 pKa = 3.71 QAYY883 pKa = 9.93 DD884 pKa = 3.41 VDD886 pKa = 4.35 EE887 pKa = 4.82 KK888 pKa = 11.57 LVMDD892 pKa = 4.7 LCAIRR897 pKa = 11.84 PKK899 pKa = 10.64 NDD901 pKa = 2.95 EE902 pKa = 4.06 AVPYY906 pKa = 9.81 PGVAFWRR913 pKa = 11.84 RR914 pKa = 11.84 GSSTTRR920 pKa = 11.84 FEE922 pKa = 4.54 DD923 pKa = 4.0 EE924 pKa = 3.72 PAFRR928 pKa = 11.84 HH929 pKa = 6.52 LFDD932 pKa = 4.57 EE933 pKa = 4.42 LQKK936 pKa = 10.91 FISSVSTLYY945 pKa = 9.75 TRR947 pKa = 11.84 KK948 pKa = 8.22 TSGGIDD954 pKa = 2.67 SWRR957 pKa = 11.84 FMEE960 pKa = 4.36 ILRR963 pKa = 11.84 GEE965 pKa = 4.14 HH966 pKa = 6.82 SIGAGQFGNDD976 pKa = 2.48 IRR978 pKa = 11.84 EE979 pKa = 4.01 WCKK982 pKa = 10.36 FLGKK986 pKa = 10.29 NPLTKK991 pKa = 10.15 SLKK994 pKa = 7.79 AARR997 pKa = 11.84 RR998 pKa = 11.84 FRR1000 pKa = 11.84 VQEE1003 pKa = 4.08 KK1004 pKa = 10.92 NPVADD1009 pKa = 5.18 DD1010 pKa = 3.58 DD1011 pKa = 4.85 TINKK1015 pKa = 9.61 AKK1017 pKa = 10.35 EE1018 pKa = 3.57 AFAWCLNFSKK1028 pKa = 9.45 EE1029 pKa = 4.24 TSFVGPVHH1037 pKa = 5.87 YY1038 pKa = 10.47 ASEE1041 pKa = 4.35 VSKK1044 pKa = 11.11 LLLEE1048 pKa = 4.53 RR1049 pKa = 11.84 AGG1051 pKa = 3.54
Molecular weight: 119.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.375
IPC2_protein 8.287
IPC_protein 8.141
Toseland 8.697
ProMoST 8.756
Dawson 9.136
Bjellqvist 9.107
Wikipedia 9.224
Rodwell 9.311
Grimsley 9.004
Solomon 9.18
Lehninger 9.151
Nozaki 9.297
DTASelect 8.946
Thurlkill 9.092
EMBOSS 9.297
Sillero 9.282
Patrickios 4.965
IPC_peptide 9.18
IPC2_peptide 7.79
IPC2.peptide.svr19 7.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1434
383
1051
717.0
81.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.717 ± 0.906
1.534 ± 0.017
6.555 ± 0.015
7.81 ± 1.929
3.975 ± 0.715
5.509 ± 0.562
2.092 ± 0.139
5.091 ± 0.616
6.555 ± 0.533
8.647 ± 1.797
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.883 ± 0.577
4.463 ± 0.013
4.184 ± 0.277
3.626 ± 0.7
9.135 ± 0.549
4.881 ± 0.369
5.37 ± 0.881
6.346 ± 0.232
1.534 ± 0.394
2.092 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here