Pantoea sp. Aalb
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 881 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4VCP0|A0A6I4VCP0_9GAMM GTPase HflX OS=Pantoea sp. Aalb OX=2576762 GN=hflX PE=3 SV=1
MM1 pKa = 7.55 NDD3 pKa = 2.66 IEE5 pKa = 4.28 QRR7 pKa = 11.84 VKK9 pKa = 10.84 KK10 pKa = 10.27 IVAEE14 pKa = 3.84 QLGVKK19 pKa = 9.35 EE20 pKa = 4.68 DD21 pKa = 3.93 EE22 pKa = 4.89 VINTASFVEE31 pKa = 4.26 DD32 pKa = 4.48 LGADD36 pKa = 3.39 SLDD39 pKa = 3.64 TVEE42 pKa = 5.9 LVMALEE48 pKa = 4.53 EE49 pKa = 4.22 EE50 pKa = 4.81 FNTEE54 pKa = 3.82 IPDD57 pKa = 3.56 EE58 pKa = 4.17 EE59 pKa = 4.47 AEE61 pKa = 5.03 KK62 pKa = 9.69 ITTVQAAIDD71 pKa = 4.38 YY72 pKa = 10.65 INSHH76 pKa = 5.54 QNN78 pKa = 2.8
Molecular weight: 8.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.316
IPC2_protein 3.935
IPC_protein 3.834
Toseland 3.668
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.668
Grimsley 3.579
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.037
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.948
Patrickios 3.694
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A6I4VGS8|A0A6I4VGS8_9GAMM 4-hydroxythreonine-4-phosphate dehydrogenase OS=Pantoea sp. Aalb OX=2576762 GN=pdxA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSILKK11 pKa = 10.3 RR12 pKa = 11.84 NRR14 pKa = 11.84 LHH16 pKa = 6.9 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.14 NGRR28 pKa = 11.84 QILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.06 GRR39 pKa = 11.84 HH40 pKa = 5.47 RR41 pKa = 11.84 LTVCC45 pKa = 4.25
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
881
0
881
290127
15
1413
329.3
37.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.14 ± 0.069
1.278 ± 0.031
4.446 ± 0.049
5.228 ± 0.074
4.212 ± 0.057
6.147 ± 0.071
2.468 ± 0.035
10.448 ± 0.092
6.8 ± 0.068
10.478 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.037
6.284 ± 0.072
3.562 ± 0.041
3.835 ± 0.052
4.784 ± 0.062
6.217 ± 0.054
4.954 ± 0.042
5.533 ± 0.066
1.148 ± 0.033
3.551 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here