Streptomyces phage Wakanda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wilnyevirus; unclassified Wilnyevirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R1G2|A0A6G8R1G2_9CAUD Holin OS=Streptomyces phage Wakanda OX=2713267 GN=47 PE=4 SV=1
MM1 pKa = 6.78VTTPSCSTKK10 pKa = 10.19MGGCSLSSQEE20 pKa = 4.49SPKK23 pKa = 10.48EE24 pKa = 4.02SPSVNEE30 pKa = 4.19TPSAFDD36 pKa = 3.57PEE38 pKa = 4.32NFNFVVLVQLMRR50 pKa = 11.84LYY52 pKa = 10.81DD53 pKa = 3.46VGMALLSCYY62 pKa = 10.21DD63 pKa = 3.7EE64 pKa = 4.55QKK66 pKa = 11.05AATLAQMHH74 pKa = 6.04EE75 pKa = 4.57AGLSFTPNPAFAVMEE90 pKa = 5.16DD91 pKa = 4.27DD92 pKa = 5.09DD93 pKa = 4.15EE94 pKa = 4.74TMASPEE100 pKa = 4.2TTPEE104 pKa = 3.98DD105 pKa = 3.45LL106 pKa = 5.04

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R1L8|A0A6G8R1L8_9CAUD Uncharacterized protein OS=Streptomyces phage Wakanda OX=2713267 GN=104 PE=4 SV=1
MM1 pKa = 7.76SMKK4 pKa = 9.72TLAKK8 pKa = 10.25SIVASALFRR17 pKa = 11.84RR18 pKa = 11.84LLAKK22 pKa = 9.32WRR24 pKa = 11.84ARR26 pKa = 11.84RR27 pKa = 11.84VRR29 pKa = 3.39

Molecular weight: 3.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

224

0

224

35477

29

2088

158.4

17.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.699 ± 0.324

1.111 ± 0.12

6.283 ± 0.144

7.069 ± 0.308

3.848 ± 0.144

7.816 ± 0.222

1.812 ± 0.101

5.023 ± 0.138

5.939 ± 0.281

7.241 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.164

4.194 ± 0.159

3.859 ± 0.203

3.044 ± 0.176

5.691 ± 0.188

5.993 ± 0.268

6.103 ± 0.35

7.47 ± 0.244

1.973 ± 0.101

4.082 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski