Amino acid dipepetide frequency for Streptomyces phage Wakanda

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.893AlaAla: 7.893 ± 0.746
0.789AlaCys: 0.789 ± 0.179
4.256AlaAsp: 4.256 ± 0.319
6.511AlaGlu: 6.511 ± 0.628
3.157AlaPhe: 3.157 ± 0.297
6.934AlaGly: 6.934 ± 0.639
1.494AlaHis: 1.494 ± 0.183
4.454AlaIle: 4.454 ± 0.347
5.243AlaLys: 5.243 ± 0.462
6.962AlaLeu: 6.962 ± 0.468
2.734AlaMet: 2.734 ± 0.371
3.467AlaAsn: 3.467 ± 0.419
3.834AlaPro: 3.834 ± 0.595
3.326AlaGln: 3.326 ± 0.402
4.51AlaArg: 4.51 ± 0.403
4.454AlaSer: 4.454 ± 0.44
5.497AlaThr: 5.497 ± 0.694
6.624AlaVal: 6.624 ± 0.358
1.832AlaTrp: 1.832 ± 0.183
3.016AlaTyr: 3.016 ± 0.277
0.0AlaXaa: 0.0 ± 0.0
Cys
0.733CysAla: 0.733 ± 0.171
0.141CysCys: 0.141 ± 0.061
0.789CysAsp: 0.789 ± 0.192
1.212CysGlu: 1.212 ± 0.223
0.564CysPhe: 0.564 ± 0.137
1.325CysGly: 1.325 ± 0.252
0.282CysHis: 0.282 ± 0.084
0.451CysIle: 0.451 ± 0.149
0.507CysLys: 0.507 ± 0.115
0.733CysLeu: 0.733 ± 0.181
0.31CysMet: 0.31 ± 0.089
0.338CysAsn: 0.338 ± 0.116
0.592CysPro: 0.592 ± 0.147
0.31CysGln: 0.31 ± 0.097
0.564CysArg: 0.564 ± 0.137
0.846CysSer: 0.846 ± 0.185
0.366CysThr: 0.366 ± 0.099
0.423CysVal: 0.423 ± 0.129
0.197CysTrp: 0.197 ± 0.068
0.423CysTyr: 0.423 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
5.271AspAla: 5.271 ± 0.338
1.156AspCys: 1.156 ± 0.234
3.383AspAsp: 3.383 ± 0.364
5.384AspGlu: 5.384 ± 0.453
2.875AspPhe: 2.875 ± 0.249
5.919AspGly: 5.919 ± 0.438
1.184AspHis: 1.184 ± 0.187
3.242AspIle: 3.242 ± 0.387
3.495AspLys: 3.495 ± 0.291
4.087AspLeu: 4.087 ± 0.354
1.945AspMet: 1.945 ± 0.242
2.706AspAsn: 2.706 ± 0.261
2.34AspPro: 2.34 ± 0.245
1.55AspGln: 1.55 ± 0.181
2.932AspArg: 2.932 ± 0.303
3.552AspSer: 3.552 ± 0.317
3.129AspThr: 3.129 ± 0.357
4.172AspVal: 4.172 ± 0.416
1.409AspTrp: 1.409 ± 0.214
3.101AspTyr: 3.101 ± 0.266
0.0AspXaa: 0.0 ± 0.0
Glu
6.032GluAla: 6.032 ± 0.47
0.62GluCys: 0.62 ± 0.158
4.595GluAsp: 4.595 ± 0.435
5.328GluGlu: 5.328 ± 0.612
3.383GluPhe: 3.383 ± 0.387
4.369GluGly: 4.369 ± 0.321
1.438GluHis: 1.438 ± 0.279
3.693GluIle: 3.693 ± 0.338
4.792GluLys: 4.792 ± 0.42
6.258GluLeu: 6.258 ± 0.485
1.776GluMet: 1.776 ± 0.282
2.706GluAsn: 2.706 ± 0.257
2.17GluPro: 2.17 ± 0.273
2.311GluGln: 2.311 ± 0.385
4.059GluArg: 4.059 ± 0.373
3.129GluSer: 3.129 ± 0.336
3.834GluThr: 3.834 ± 0.38
5.328GluVal: 5.328 ± 0.393
1.579GluTrp: 1.579 ± 0.218
3.298GluTyr: 3.298 ± 0.393
0.0GluXaa: 0.0 ± 0.0
Phe
3.072PheAla: 3.072 ± 0.366
0.507PheCys: 0.507 ± 0.127
3.129PheAsp: 3.129 ± 0.303
3.129PheGlu: 3.129 ± 0.34
1.381PhePhe: 1.381 ± 0.186
3.016PheGly: 3.016 ± 0.234
0.592PheHis: 0.592 ± 0.135
1.719PheIle: 1.719 ± 0.225
2.283PheLys: 2.283 ± 0.265
2.762PheLeu: 2.762 ± 0.36
1.015PheMet: 1.015 ± 0.177
1.55PheAsn: 1.55 ± 0.219
1.381PhePro: 1.381 ± 0.171
1.099PheGln: 1.099 ± 0.214
2.142PheArg: 2.142 ± 0.274
2.593PheSer: 2.593 ± 0.436
2.396PheThr: 2.396 ± 0.228
2.593PheVal: 2.593 ± 0.308
0.789PheTrp: 0.789 ± 0.128
1.325PheTyr: 1.325 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
5.638GlyAla: 5.638 ± 0.436
0.846GlyCys: 0.846 ± 0.161
5.074GlyAsp: 5.074 ± 0.404
3.946GlyGlu: 3.946 ± 0.421
3.185GlyPhe: 3.185 ± 0.369
5.976GlyGly: 5.976 ± 0.605
1.776GlyHis: 1.776 ± 0.215
4.707GlyIle: 4.707 ± 0.326
5.102GlyLys: 5.102 ± 0.503
6.089GlyLeu: 6.089 ± 0.441
2.368GlyMet: 2.368 ± 0.222
3.749GlyAsn: 3.749 ± 0.584
2.424GlyPro: 2.424 ± 0.324
2.03GlyGln: 2.03 ± 0.269
4.285GlyArg: 4.285 ± 0.335
4.679GlySer: 4.679 ± 0.508
4.989GlyThr: 4.989 ± 0.749
5.497GlyVal: 5.497 ± 0.395
2.086GlyTrp: 2.086 ± 0.272
3.721GlyTyr: 3.721 ± 0.33
0.0GlyXaa: 0.0 ± 0.0
His
1.522HisAla: 1.522 ± 0.245
0.197HisCys: 0.197 ± 0.076
1.297HisAsp: 1.297 ± 0.227
1.128HisGlu: 1.128 ± 0.215
0.564HisPhe: 0.564 ± 0.146
1.466HisGly: 1.466 ± 0.202
0.423HisHis: 0.423 ± 0.105
0.902HisIle: 0.902 ± 0.151
1.55HisLys: 1.55 ± 0.233
1.24HisLeu: 1.24 ± 0.195
0.733HisMet: 0.733 ± 0.14
0.677HisAsn: 0.677 ± 0.127
0.705HisPro: 0.705 ± 0.14
0.564HisGln: 0.564 ± 0.148
1.184HisArg: 1.184 ± 0.195
0.958HisSer: 0.958 ± 0.152
0.761HisThr: 0.761 ± 0.125
0.987HisVal: 0.987 ± 0.186
0.338HisTrp: 0.338 ± 0.101
0.93HisTyr: 0.93 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
4.51IleAla: 4.51 ± 0.391
0.761IleCys: 0.761 ± 0.149
3.89IleAsp: 3.89 ± 0.402
3.862IleGlu: 3.862 ± 0.393
1.099IlePhe: 1.099 ± 0.176
3.89IleGly: 3.89 ± 0.364
0.902IleHis: 0.902 ± 0.143
2.34IleIle: 2.34 ± 0.256
2.734IleLys: 2.734 ± 0.272
3.946IleLeu: 3.946 ± 0.319
1.353IleMet: 1.353 ± 0.181
1.889IleAsn: 1.889 ± 0.242
2.058IlePro: 2.058 ± 0.265
1.663IleGln: 1.663 ± 0.182
3.213IleArg: 3.213 ± 0.277
3.101IleSer: 3.101 ± 0.31
3.129IleThr: 3.129 ± 0.305
3.439IleVal: 3.439 ± 0.323
0.761IleTrp: 0.761 ± 0.147
1.691IleTyr: 1.691 ± 0.23
0.0IleXaa: 0.0 ± 0.0
Lys
6.23LysAla: 6.23 ± 0.572
0.451LysCys: 0.451 ± 0.122
3.016LysAsp: 3.016 ± 0.281
3.129LysGlu: 3.129 ± 0.36
2.17LysPhe: 2.17 ± 0.241
3.862LysGly: 3.862 ± 0.396
1.381LysHis: 1.381 ± 0.209
2.621LysIle: 2.621 ± 0.226
4.256LysLys: 4.256 ± 0.404
3.129LysLeu: 3.129 ± 0.252
1.973LysMet: 1.973 ± 0.211
2.791LysAsn: 2.791 ± 0.341
2.537LysPro: 2.537 ± 0.316
1.945LysGln: 1.945 ± 0.218
4.341LysArg: 4.341 ± 0.469
3.777LysSer: 3.777 ± 0.312
3.383LysThr: 3.383 ± 0.327
4.764LysVal: 4.764 ± 0.45
1.128LysTrp: 1.128 ± 0.189
2.509LysTyr: 2.509 ± 0.288
0.0LysXaa: 0.0 ± 0.0
Leu
6.991LeuAla: 6.991 ± 0.509
0.677LeuCys: 0.677 ± 0.163
5.497LeuAsp: 5.497 ± 0.37
5.468LeuGlu: 5.468 ± 0.379
2.903LeuPhe: 2.903 ± 0.323
5.299LeuGly: 5.299 ± 0.454
1.156LeuHis: 1.156 ± 0.188
3.834LeuIle: 3.834 ± 0.338
3.805LeuLys: 3.805 ± 0.286
4.707LeuLeu: 4.707 ± 0.422
2.199LeuMet: 2.199 ± 0.279
3.27LeuAsn: 3.27 ± 0.322
2.368LeuPro: 2.368 ± 0.231
2.03LeuGln: 2.03 ± 0.366
4.115LeuArg: 4.115 ± 0.354
4.369LeuSer: 4.369 ± 0.34
4.313LeuThr: 4.313 ± 0.501
5.609LeuVal: 5.609 ± 0.47
1.212LeuTrp: 1.212 ± 0.209
2.593LeuTyr: 2.593 ± 0.289
0.0LeuXaa: 0.0 ± 0.0
Met
3.016MetAla: 3.016 ± 0.35
0.254MetCys: 0.254 ± 0.087
1.128MetAsp: 1.128 ± 0.19
1.691MetGlu: 1.691 ± 0.212
0.817MetPhe: 0.817 ± 0.176
1.945MetGly: 1.945 ± 0.297
0.564MetHis: 0.564 ± 0.13
1.156MetIle: 1.156 ± 0.221
1.691MetLys: 1.691 ± 0.231
1.719MetLeu: 1.719 ± 0.25
0.733MetMet: 0.733 ± 0.165
1.015MetAsn: 1.015 ± 0.156
1.043MetPro: 1.043 ± 0.167
0.958MetGln: 0.958 ± 0.181
1.86MetArg: 1.86 ± 0.24
2.34MetSer: 2.34 ± 0.263
2.199MetThr: 2.199 ± 0.25
1.832MetVal: 1.832 ± 0.213
0.733MetTrp: 0.733 ± 0.132
0.817MetTyr: 0.817 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
3.524AsnAla: 3.524 ± 0.384
0.366AsnCys: 0.366 ± 0.094
2.481AsnAsp: 2.481 ± 0.26
2.96AsnGlu: 2.96 ± 0.358
1.522AsnPhe: 1.522 ± 0.233
4.285AsnGly: 4.285 ± 0.438
0.987AsnHis: 0.987 ± 0.16
2.001AsnIle: 2.001 ± 0.218
2.537AsnLys: 2.537 ± 0.283
2.932AsnLeu: 2.932 ± 0.354
1.071AsnMet: 1.071 ± 0.182
1.268AsnAsn: 1.268 ± 0.235
2.762AsnPro: 2.762 ± 0.307
1.015AsnGln: 1.015 ± 0.174
2.424AsnArg: 2.424 ± 0.264
2.424AsnSer: 2.424 ± 0.412
2.283AsnThr: 2.283 ± 0.335
2.875AsnVal: 2.875 ± 0.289
0.817AsnTrp: 0.817 ± 0.164
1.409AsnTyr: 1.409 ± 0.197
0.0AsnXaa: 0.0 ± 0.0
Pro
3.383ProAla: 3.383 ± 0.504
0.366ProCys: 0.366 ± 0.085
3.129ProAsp: 3.129 ± 0.344
2.847ProGlu: 2.847 ± 0.328
1.268ProPhe: 1.268 ± 0.159
3.242ProGly: 3.242 ± 0.351
0.479ProHis: 0.479 ± 0.099
1.889ProIle: 1.889 ± 0.279
2.396ProLys: 2.396 ± 0.299
2.283ProLeu: 2.283 ± 0.21
0.733ProMet: 0.733 ± 0.159
1.776ProAsn: 1.776 ± 0.264
1.409ProPro: 1.409 ± 0.213
1.156ProGln: 1.156 ± 0.218
2.199ProArg: 2.199 ± 0.301
2.734ProSer: 2.734 ± 0.351
2.481ProThr: 2.481 ± 0.359
3.072ProVal: 3.072 ± 0.309
0.395ProTrp: 0.395 ± 0.102
1.353ProTyr: 1.353 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
3.411GlnAla: 3.411 ± 0.446
0.366GlnCys: 0.366 ± 0.09
1.522GlnAsp: 1.522 ± 0.217
2.058GlnGlu: 2.058 ± 0.262
1.184GlnPhe: 1.184 ± 0.181
1.945GlnGly: 1.945 ± 0.241
0.395GlnHis: 0.395 ± 0.098
1.804GlnIle: 1.804 ± 0.207
1.889GlnLys: 1.889 ± 0.291
2.424GlnLeu: 2.424 ± 0.272
0.564GlnMet: 0.564 ± 0.164
1.297GlnAsn: 1.297 ± 0.185
1.099GlnPro: 1.099 ± 0.22
1.24GlnGln: 1.24 ± 0.227
1.973GlnArg: 1.973 ± 0.289
1.86GlnSer: 1.86 ± 0.209
1.494GlnThr: 1.494 ± 0.215
2.283GlnVal: 2.283 ± 0.278
0.761GlnTrp: 0.761 ± 0.131
0.874GlnTyr: 0.874 ± 0.151
0.0GlnXaa: 0.0 ± 0.0
Arg
5.215ArgAla: 5.215 ± 0.416
0.705ArgCys: 0.705 ± 0.151
3.298ArgAsp: 3.298 ± 0.384
4.595ArgGlu: 4.595 ± 0.517
2.17ArgPhe: 2.17 ± 0.228
3.975ArgGly: 3.975 ± 0.362
0.846ArgHis: 0.846 ± 0.165
3.721ArgIle: 3.721 ± 0.267
3.552ArgLys: 3.552 ± 0.433
4.397ArgLeu: 4.397 ± 0.348
1.635ArgMet: 1.635 ± 0.224
2.311ArgAsn: 2.311 ± 0.268
2.058ArgPro: 2.058 ± 0.251
1.635ArgGln: 1.635 ± 0.232
3.439ArgArg: 3.439 ± 0.429
2.452ArgSer: 2.452 ± 0.247
2.621ArgThr: 2.621 ± 0.259
4.285ArgVal: 4.285 ± 0.341
1.099ArgTrp: 1.099 ± 0.172
2.903ArgTyr: 2.903 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
4.933SerAla: 4.933 ± 0.481
0.789SerCys: 0.789 ± 0.157
3.946SerAsp: 3.946 ± 0.355
3.157SerGlu: 3.157 ± 0.29
2.875SerPhe: 2.875 ± 0.3
5.694SerGly: 5.694 ± 0.829
1.128SerHis: 1.128 ± 0.186
2.311SerIle: 2.311 ± 0.256
3.129SerLys: 3.129 ± 0.359
4.764SerLeu: 4.764 ± 0.375
1.494SerMet: 1.494 ± 0.236
2.678SerAsn: 2.678 ± 0.256
2.424SerPro: 2.424 ± 0.343
2.086SerGln: 2.086 ± 0.231
2.791SerArg: 2.791 ± 0.34
3.946SerSer: 3.946 ± 0.799
4.087SerThr: 4.087 ± 0.524
4.313SerVal: 4.313 ± 0.422
1.043SerTrp: 1.043 ± 0.175
2.34SerTyr: 2.34 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
5.609ThrAla: 5.609 ± 0.76
0.451ThrCys: 0.451 ± 0.116
3.749ThrAsp: 3.749 ± 0.429
3.89ThrGlu: 3.89 ± 0.343
2.311ThrPhe: 2.311 ± 0.29
5.187ThrGly: 5.187 ± 0.704
0.987ThrHis: 0.987 ± 0.189
2.875ThrIle: 2.875 ± 0.366
2.65ThrLys: 2.65 ± 0.263
4.933ThrLeu: 4.933 ± 0.404
1.438ThrMet: 1.438 ± 0.212
2.903ThrAsn: 2.903 ± 0.44
2.875ThrPro: 2.875 ± 0.401
1.804ThrGln: 1.804 ± 0.249
2.706ThrArg: 2.706 ± 0.284
3.411ThrSer: 3.411 ± 0.504
4.707ThrThr: 4.707 ± 0.748
5.017ThrVal: 5.017 ± 0.549
1.325ThrTrp: 1.325 ± 0.21
2.199ThrTyr: 2.199 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
5.638ValAla: 5.638 ± 0.408
0.874ValCys: 0.874 ± 0.17
4.566ValAsp: 4.566 ± 0.401
5.835ValGlu: 5.835 ± 0.516
2.903ValPhe: 2.903 ± 0.285
4.792ValGly: 4.792 ± 0.372
1.015ValHis: 1.015 ± 0.204
4.003ValIle: 4.003 ± 0.353
4.989ValLys: 4.989 ± 0.436
4.623ValLeu: 4.623 ± 0.394
1.635ValMet: 1.635 ± 0.218
2.96ValAsn: 2.96 ± 0.283
2.791ValPro: 2.791 ± 0.356
2.142ValGln: 2.142 ± 0.233
4.51ValArg: 4.51 ± 0.361
4.905ValSer: 4.905 ± 0.418
5.158ValThr: 5.158 ± 0.472
6.793ValVal: 6.793 ± 0.553
1.297ValTrp: 1.297 ± 0.248
3.242ValTyr: 3.242 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.55TrpAla: 1.55 ± 0.231
0.338TrpCys: 0.338 ± 0.101
1.381TrpAsp: 1.381 ± 0.204
1.635TrpGlu: 1.635 ± 0.232
0.648TrpPhe: 0.648 ± 0.163
1.466TrpGly: 1.466 ± 0.24
0.338TrpHis: 0.338 ± 0.105
0.761TrpIle: 0.761 ± 0.144
1.071TrpLys: 1.071 ± 0.174
1.579TrpLeu: 1.579 ± 0.272
0.677TrpMet: 0.677 ± 0.145
0.958TrpAsn: 0.958 ± 0.21
0.564TrpPro: 0.564 ± 0.139
0.564TrpGln: 0.564 ± 0.142
1.099TrpArg: 1.099 ± 0.163
1.466TrpSer: 1.466 ± 0.177
1.268TrpThr: 1.268 ± 0.321
1.297TrpVal: 1.297 ± 0.203
0.282TrpTrp: 0.282 ± 0.1
0.789TrpTyr: 0.789 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.819TyrAla: 2.819 ± 0.32
0.451TyrCys: 0.451 ± 0.109
2.706TyrAsp: 2.706 ± 0.238
2.932TyrGlu: 2.932 ± 0.326
1.494TyrPhe: 1.494 ± 0.23
3.58TyrGly: 3.58 ± 0.297
0.761TyrHis: 0.761 ± 0.133
1.748TyrIle: 1.748 ± 0.228
1.719TyrLys: 1.719 ± 0.231
2.847TyrLeu: 2.847 ± 0.273
1.099TyrMet: 1.099 ± 0.185
1.635TyrAsn: 1.635 ± 0.204
1.156TyrPro: 1.156 ± 0.168
1.015TyrGln: 1.015 ± 0.176
2.565TyrArg: 2.565 ± 0.254
2.932TyrSer: 2.932 ± 0.324
2.932TyrThr: 2.932 ± 0.3
3.495TyrVal: 3.495 ± 0.406
0.648TyrTrp: 0.648 ± 0.154
2.283TyrTyr: 2.283 ± 0.332
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 224 proteins (35477 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski