Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7FRB3|K7FRB3_PELSI Solute carrier family 11 member 2 OS=Pelodiscus sinensis OX=13735 GN=SLC11A2 PE=3 SV=1
MM1 pKa = 8.37DD2 pKa = 5.04DD3 pKa = 3.3TTNNQFSYY11 pKa = 9.39VTHH14 pKa = 7.13EE15 pKa = 5.05DD16 pKa = 2.66ICNCFNGDD24 pKa = 3.43TLLAIQAPCGTQLEE38 pKa = 4.63VPIPEE43 pKa = 4.4MGQNGQKK50 pKa = 10.21KK51 pKa = 8.37YY52 pKa = 10.6QISLKK57 pKa = 10.33SSSGPIQVLLINKK70 pKa = 7.64EE71 pKa = 4.42SSSSTPVVFPVPPPDD86 pKa = 6.27DD87 pKa = 3.32IAQPPSQLATPVTPPKK103 pKa = 9.34PTATTEE109 pKa = 4.13NTSEE113 pKa = 3.84QHH115 pKa = 6.05SVNEE119 pKa = 4.35GQHH122 pKa = 5.97LPRR125 pKa = 11.84TTDD128 pKa = 3.24TPSDD132 pKa = 3.23NSAQEE137 pKa = 3.91NTTPEE142 pKa = 4.2TPYY145 pKa = 11.53SNLPDD150 pKa = 3.53SLLYY154 pKa = 7.89TTLATDD160 pKa = 4.12TTQTTVNSNDD170 pKa = 3.51YY171 pKa = 10.43QALLPLDD178 pKa = 4.05VNCILKK184 pKa = 10.4PNSLDD189 pKa = 3.35MAKK192 pKa = 9.27MDD194 pKa = 4.58EE195 pKa = 4.24PTGTISSDD203 pKa = 3.37IIDD206 pKa = 4.36EE207 pKa = 4.39LMSSDD212 pKa = 3.97VFPLLRR218 pKa = 11.84LSPTPGDD225 pKa = 3.86DD226 pKa = 3.93YY227 pKa = 11.79NFNLDD232 pKa = 4.1DD233 pKa = 4.45SEE235 pKa = 4.88GVCDD239 pKa = 5.38LFDD242 pKa = 3.89VQILNYY248 pKa = 10.41

Molecular weight: 26.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7F0I4|K7F0I4_PELSI Reverse transcriptase domain-containing protein OS=Pelodiscus sinensis OX=13735 PE=4 SV=1
MM1 pKa = 7.66PGSPARR7 pKa = 11.84PGQWGLVGGAGGLGGRR23 pKa = 11.84MPGSPARR30 pKa = 11.84PGQWGLVGGAGGLGGRR46 pKa = 11.84MPGSPARR53 pKa = 11.84PGQWGLVGGAGGLGGRR69 pKa = 11.84MPGSPARR76 pKa = 11.84PGQWGLVGGAGGLGGRR92 pKa = 11.84MPGSPARR99 pKa = 11.84PGQWGLVGGAGGLGGRR115 pKa = 11.84MPGSPARR122 pKa = 11.84PGQWGLVGGAGGLGGRR138 pKa = 11.84MPGSPARR145 pKa = 11.84PGQWGLVGGAGGLGGRR161 pKa = 11.84MPGSPARR168 pKa = 11.84PGQWGLVGGAGGLGGRR184 pKa = 11.84MPGSPARR191 pKa = 11.84PGQWGLVGGAGGLGGRR207 pKa = 11.84MPGSPARR214 pKa = 11.84PGQWGLVGGAGGLGGRR230 pKa = 11.84MPGSPARR237 pKa = 11.84PGQWGLVGGAGGLGGRR253 pKa = 11.84MPGSPARR260 pKa = 11.84PGQWGLVGGAGGLGGRR276 pKa = 11.84MPGSPARR283 pKa = 11.84PGQGAGQGCGAGPRR297 pKa = 11.84LHH299 pKa = 6.87GGGAVSHH306 pKa = 6.26LRR308 pKa = 11.84GAGSPHH314 pKa = 5.63QGVWASFSSRR324 pKa = 11.84SSS326 pKa = 3.04

Molecular weight: 30.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18111

2398

20509

10850553

20

34915

529.1

59.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.415 ± 0.016

2.349 ± 0.016

4.888 ± 0.011

7.025 ± 0.022

3.783 ± 0.011

6.127 ± 0.026

2.607 ± 0.01

4.925 ± 0.012

6.281 ± 0.02

9.67 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.008

4.077 ± 0.012

5.537 ± 0.026

4.683 ± 0.017

5.354 ± 0.016

8.269 ± 0.023

5.464 ± 0.017

6.122 ± 0.018

1.24 ± 0.006

2.861 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski