Amino acid dipepetide frequency for Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.593AlaAla: 5.593 ± 0.04	1.385AlaCys: 1.385 ± 0.017	2.866AlaAsp: 2.866 ± 0.02	4.413AlaGlu: 4.413 ± 0.032	2.566AlaPhe: 2.566 ± 0.016	4.169AlaGly: 4.169 ± 0.037	1.449AlaHis: 1.449 ± 0.015	2.985AlaIle: 2.985 ± 0.017	3.551AlaLys: 3.551 ± 0.026	6.436AlaLeu: 6.436 ± 0.038	1.515AlaMet: 1.515 ± 0.012	2.225AlaAsn: 2.225 ± 0.015	3.404AlaPro: 3.404 ± 0.038	2.85AlaGln: 2.85 ± 0.023	3.153AlaArg: 3.153 ± 0.032	5.203AlaSer: 5.203 ± 0.032	3.442AlaThr: 3.442 ± 0.022	4.652AlaVal: 4.652 ± 0.027	0.751AlaTrp: 0.751 ± 0.013	1.539AlaTyr: 1.539 ± 0.014	0.008AlaXaa: 0.008 ± 0.001
Cys	1.285CysAla: 1.285 ± 0.016	0.674CysCys: 0.674 ± 0.017	1.05CysAsp: 1.05 ± 0.015	1.297CysGlu: 1.297 ± 0.015	0.908CysPhe: 0.908 ± 0.012	1.757CysGly: 1.757 ± 0.031	0.67CysHis: 0.67 ± 0.011	1.155CysIle: 1.155 ± 0.015	1.344CysLys: 1.344 ± 0.014	2.268CysLeu: 2.268 ± 0.023	0.472CysMet: 0.472 ± 0.007	0.906CysAsn: 0.906 ± 0.011	1.377CysPro: 1.377 ± 0.023	1.116CysGln: 1.116 ± 0.015	1.277CysArg: 1.277 ± 0.018	2.173CysSer: 2.173 ± 0.023	1.262CysThr: 1.262 ± 0.014	1.509CysVal: 1.509 ± 0.029	0.347CysTrp: 0.347 ± 0.011	0.636CysTyr: 0.636 ± 0.008	0.002CysXaa: 0.002 ± 0.0
Asp	2.684AspAla: 2.684 ± 0.016	1.216AspCys: 1.216 ± 0.018	2.622AspAsp: 2.622 ± 0.024	3.457AspGlu: 3.457 ± 0.023	2.247AspPhe: 2.247 ± 0.016	3.122AspGly: 3.122 ± 0.026	1.17AspHis: 1.17 ± 0.012	2.891AspIle: 2.891 ± 0.023	2.734AspLys: 2.734 ± 0.02	4.906AspLeu: 4.906 ± 0.029	1.172AspMet: 1.172 ± 0.012	1.888AspAsn: 1.888 ± 0.017	2.612AspPro: 2.612 ± 0.022	1.805AspGln: 1.805 ± 0.014	2.425AspArg: 2.425 ± 0.019	4.044AspSer: 4.044 ± 0.026	2.532AspThr: 2.532 ± 0.021	3.097AspVal: 3.097 ± 0.027	0.695AspTrp: 0.695 ± 0.01	1.557AspTyr: 1.557 ± 0.015	0.002AspXaa: 0.002 ± 0.001
Glu	4.534GluAla: 4.534 ± 0.032	1.392GluCys: 1.392 ± 0.021	4.223GluAsp: 4.223 ± 0.025	7.571GluGlu: 7.571 ± 0.062	2.143GluPhe: 2.143 ± 0.015	3.967GluGly: 3.967 ± 0.031	1.504GluHis: 1.504 ± 0.016	3.587GluIle: 3.587 ± 0.023	5.592GluLys: 5.592 ± 0.044	6.204GluLeu: 6.204 ± 0.037	1.807GluMet: 1.807 ± 0.016	3.37GluAsn: 3.37 ± 0.023	2.67GluPro: 2.67 ± 0.029	3.133GluGln: 3.133 ± 0.023	3.949GluArg: 3.949 ± 0.03	4.471GluSer: 4.471 ± 0.027	3.577GluThr: 3.577 ± 0.024	4.091GluVal: 4.091 ± 0.026	0.73GluTrp: 0.73 ± 0.012	1.729GluTyr: 1.729 ± 0.018	0.005GluXaa: 0.005 ± 0.001
Phe	2.054PheAla: 2.054 ± 0.016	1.001PheCys: 1.001 ± 0.01	1.765PheAsp: 1.765 ± 0.015	2.009PheGlu: 2.009 ± 0.014	1.745PhePhe: 1.745 ± 0.019	2.181PheGly: 2.181 ± 0.017	1.063PheHis: 1.063 ± 0.01	2.029PheIle: 2.029 ± 0.018	2.049PheLys: 2.049 ± 0.017	4.14PheLeu: 4.14 ± 0.034	0.799PheMet: 0.799 ± 0.009	1.572PheAsn: 1.572 ± 0.015	1.942PhePro: 1.942 ± 0.02	1.826PheGln: 1.826 ± 0.015	1.863PheArg: 1.863 ± 0.016	3.541PheSer: 3.541 ± 0.027	2.233PheThr: 2.233 ± 0.016	2.22PheVal: 2.22 ± 0.017	0.507PheTrp: 0.507 ± 0.007	1.287PheTyr: 1.287 ± 0.014	0.003PheXaa: 0.003 ± 0.0
Gly	3.864GlyAla: 3.864 ± 0.033	1.311GlyCys: 1.311 ± 0.019	2.989GlyAsp: 2.989 ± 0.024	4.116GlyGlu: 4.116 ± 0.037	2.408GlyPhe: 2.408 ± 0.018	4.286GlyGly: 4.286 ± 0.043	1.544GlyHis: 1.544 ± 0.016	3.045GlyIle: 3.045 ± 0.022	4.222GlyLys: 4.222 ± 0.032	5.315GlyLeu: 5.315 ± 0.034	1.343GlyMet: 1.343 ± 0.015	2.704GlyAsn: 2.704 ± 0.021	3.178GlyPro: 3.178 ± 0.057	2.595GlyGln: 2.595 ± 0.023	3.435GlyArg: 3.435 ± 0.033	5.259GlySer: 5.259 ± 0.034	3.502GlyThr: 3.502 ± 0.028	3.51GlyVal: 3.51 ± 0.029	0.798GlyTrp: 0.798 ± 0.011	1.838GlyTyr: 1.838 ± 0.02	0.009GlyXaa: 0.009 ± 0.001
His	1.323HisAla: 1.323 ± 0.012	0.763HisCys: 0.763 ± 0.009	0.932HisAsp: 0.932 ± 0.009	1.353HisGlu: 1.353 ± 0.014	1.097HisPhe: 1.097 ± 0.012	1.527HisGly: 1.527 ± 0.016	0.826HisHis: 0.826 ± 0.012	1.295HisIle: 1.295 ± 0.012	1.421HisLys: 1.421 ± 0.013	2.878HisLeu: 2.878 ± 0.024	0.59HisMet: 0.59 ± 0.007	1.007HisAsn: 1.007 ± 0.012	1.611HisPro: 1.611 ± 0.023	1.33HisGln: 1.33 ± 0.017	1.58HisArg: 1.58 ± 0.021	2.214HisSer: 2.214 ± 0.02	1.653HisThr: 1.653 ± 0.03	1.461HisVal: 1.461 ± 0.013	0.358HisTrp: 0.358 ± 0.006	0.851HisTyr: 0.851 ± 0.009	0.002HisXaa: 0.002 ± 0.0
Ile	2.883IleAla: 2.883 ± 0.018	1.272IleCys: 1.272 ± 0.014	2.278IleAsp: 2.278 ± 0.018	2.887IleGlu: 2.887 ± 0.025	2.104IlePhe: 2.104 ± 0.019	2.511IleGly: 2.511 ± 0.019	1.498IleHis: 1.498 ± 0.017	2.745IleIle: 2.745 ± 0.023	3.077IleLys: 3.077 ± 0.021	4.934IleLeu: 4.934 ± 0.029	1.114IleMet: 1.114 ± 0.012	2.141IleAsn: 2.141 ± 0.017	2.829IlePro: 2.829 ± 0.02	2.435IleGln: 2.435 ± 0.017	2.551IleArg: 2.551 ± 0.016	4.09IleSer: 4.09 ± 0.03	2.883IleThr: 2.883 ± 0.023	2.869IleVal: 2.869 ± 0.02	0.566IleTrp: 0.566 ± 0.01	1.586IleTyr: 1.586 ± 0.015	0.003IleXaa: 0.003 ± 0.0
Lys	4.04LysAla: 4.04 ± 0.026	1.334LysCys: 1.334 ± 0.017	3.341LysAsp: 3.341 ± 0.026	5.47LysGlu: 5.47 ± 0.039	1.84LysPhe: 1.84 ± 0.017	3.616LysGly: 3.616 ± 0.036	1.575LysHis: 1.575 ± 0.015	3.143LysIle: 3.143 ± 0.022	5.106LysLys: 5.106 ± 0.038	5.71LysLeu: 5.71 ± 0.033	1.575LysMet: 1.575 ± 0.016	2.755LysAsn: 2.755 ± 0.02	3.055LysPro: 3.055 ± 0.028	2.848LysGln: 2.848 ± 0.019	3.503LysArg: 3.503 ± 0.023	4.387LysSer: 4.387 ± 0.03	3.321LysThr: 3.321 ± 0.021	3.634LysVal: 3.634 ± 0.027	0.721LysTrp: 0.721 ± 0.011	1.825LysTyr: 1.825 ± 0.016	0.006LysXaa: 0.006 ± 0.001
Leu	6.299LeuAla: 6.299 ± 0.031	2.255LeuCys: 2.255 ± 0.022	4.764LeuAsp: 4.764 ± 0.027	6.836LeuGlu: 6.836 ± 0.041	3.521LeuPhe: 3.521 ± 0.026	5.418LeuGly: 5.418 ± 0.035	2.763LeuHis: 2.763 ± 0.022	4.279LeuIle: 4.279 ± 0.026	6.212LeuLys: 6.212 ± 0.033	10.0LeuLeu: 10.0 ± 0.056	2.012LeuMet: 2.012 ± 0.018	3.777LeuAsn: 3.777 ± 0.023	5.45LeuPro: 5.45 ± 0.034	5.57LeuGln: 5.57 ± 0.034	5.26LeuArg: 5.26 ± 0.029	7.914LeuSer: 7.914 ± 0.036	5.077LeuThr: 5.077 ± 0.026	5.478LeuVal: 5.478 ± 0.03	1.126LeuTrp: 1.126 ± 0.014	2.68LeuTyr: 2.68 ± 0.021	0.009LeuXaa: 0.009 ± 0.001
Met	1.753MetAla: 1.753 ± 0.016	0.453MetCys: 0.453 ± 0.007	1.287MetAsp: 1.287 ± 0.011	1.933MetGlu: 1.933 ± 0.015	0.784MetPhe: 0.784 ± 0.009	1.314MetGly: 1.314 ± 0.016	0.542MetHis: 0.542 ± 0.008	1.041MetIle: 1.041 ± 0.012	1.599MetLys: 1.599 ± 0.013	2.061MetLeu: 2.061 ± 0.017	0.583MetMet: 0.583 ± 0.008	1.007MetAsn: 1.007 ± 0.011	1.058MetPro: 1.058 ± 0.017	1.008MetGln: 1.008 ± 0.01	1.05MetArg: 1.05 ± 0.01	1.628MetSer: 1.628 ± 0.013	1.156MetThr: 1.156 ± 0.011	1.406MetVal: 1.406 ± 0.013	0.248MetTrp: 0.248 ± 0.004	0.648MetTyr: 0.648 ± 0.009	0.001MetXaa: 0.001 ± 0.0
Asn	2.216AsnAla: 2.216 ± 0.018	0.983AsnCys: 0.983 ± 0.01	1.711AsnAsp: 1.711 ± 0.017	2.505AsnGlu: 2.505 ± 0.017	1.578AsnPhe: 1.578 ± 0.014	2.68AsnGly: 2.68 ± 0.021	1.031AsnHis: 1.031 ± 0.012	2.512AsnIle: 2.512 ± 0.02	2.658AsnLys: 2.658 ± 0.02	4.036AsnLeu: 4.036 ± 0.027	1.025AsnMet: 1.025 ± 0.01	1.831AsnAsn: 1.831 ± 0.015	2.247AsnPro: 2.247 ± 0.016	1.769AsnGln: 1.769 ± 0.014	2.043AsnArg: 2.043 ± 0.016	3.4AsnSer: 3.4 ± 0.023	2.243AsnThr: 2.243 ± 0.017	2.472AsnVal: 2.472 ± 0.018	0.512AsnTrp: 0.512 ± 0.007	1.31AsnTyr: 1.31 ± 0.013	0.004AsnXaa: 0.004 ± 0.0
Pro	4.045ProAla: 4.045 ± 0.035	1.158ProCys: 1.158 ± 0.024	2.559ProAsp: 2.559 ± 0.023	3.806ProGlu: 3.806 ± 0.032	1.98ProPhe: 1.98 ± 0.017	4.276ProGly: 4.276 ± 0.071	1.41ProHis: 1.41 ± 0.019	2.016ProIle: 2.016 ± 0.02	2.694ProLys: 2.694 ± 0.04	4.85ProLeu: 4.85 ± 0.032	1.0ProMet: 1.0 ± 0.011	1.88ProAsn: 1.88 ± 0.016	4.793ProPro: 4.793 ± 0.068	2.452ProGln: 2.452 ± 0.023	2.782ProArg: 2.782 ± 0.041	5.054ProSer: 5.054 ± 0.04	2.764ProThr: 2.764 ± 0.028	3.693ProVal: 3.693 ± 0.03	0.669ProTrp: 0.669 ± 0.012	1.483ProTyr: 1.483 ± 0.018	0.009ProXaa: 0.009 ± 0.001
Gln	3.055GlnAla: 3.055 ± 0.021	1.017GlnCys: 1.017 ± 0.013	2.188GlnAsp: 2.188 ± 0.017	3.677GlnGlu: 3.677 ± 0.027	1.48GlnPhe: 1.48 ± 0.014	2.678GlnGly: 2.678 ± 0.03	1.329GlnHis: 1.329 ± 0.014	2.177GlnIle: 2.177 ± 0.018	3.055GlnLys: 3.055 ± 0.021	4.659GlnLeu: 4.659 ± 0.03	1.132GlnMet: 1.132 ± 0.011	2.048GlnAsn: 2.048 ± 0.016	2.444GlnPro: 2.444 ± 0.024	2.992GlnGln: 2.992 ± 0.036	2.822GlnArg: 2.822 ± 0.025	3.196GlnSer: 3.196 ± 0.024	2.429GlnThr: 2.429 ± 0.017	2.674GlnVal: 2.674 ± 0.018	0.541GlnTrp: 0.541 ± 0.012	1.235GlnTyr: 1.235 ± 0.012	0.006GlnXaa: 0.006 ± 0.001
Arg	3.382ArgAla: 3.382 ± 0.034	1.17ArgCys: 1.17 ± 0.017	2.559ArgAsp: 2.559 ± 0.018	3.778ArgGlu: 3.778 ± 0.022	1.845ArgPhe: 1.845 ± 0.016	3.278ArgGly: 3.278 ± 0.039	1.507ArgHis: 1.507 ± 0.016	2.625ArgIle: 2.625 ± 0.018	3.802ArgLys: 3.802 ± 0.024	4.841ArgLeu: 4.841 ± 0.029	1.205ArgMet: 1.205 ± 0.012	2.202ArgAsn: 2.202 ± 0.016	2.844ArgPro: 2.844 ± 0.037	2.496ArgGln: 2.496 ± 0.023	3.821ArgArg: 3.821 ± 0.037	4.126ArgSer: 4.126 ± 0.039	2.785ArgThr: 2.785 ± 0.023	3.027ArgVal: 3.027 ± 0.033	0.712ArgTrp: 0.712 ± 0.01	1.531ArgTyr: 1.531 ± 0.016	0.007ArgXaa: 0.007 ± 0.001
Ser	5.151SerAla: 5.151 ± 0.033	2.011SerCys: 2.011 ± 0.017	4.066SerAsp: 4.066 ± 0.032	5.103SerGlu: 5.103 ± 0.034	3.154SerPhe: 3.154 ± 0.024	5.075SerGly: 5.075 ± 0.03	2.136SerHis: 2.136 ± 0.02	3.544SerIle: 3.544 ± 0.022	4.394SerLys: 4.394 ± 0.025	8.106SerLeu: 8.106 ± 0.037	1.66SerMet: 1.66 ± 0.015	3.023SerAsn: 3.023 ± 0.022	5.414SerPro: 5.414 ± 0.053	3.81SerGln: 3.81 ± 0.026	4.198SerArg: 4.198 ± 0.033	8.92SerSer: 8.92 ± 0.057	4.581SerThr: 4.581 ± 0.032	5.035SerVal: 5.035 ± 0.032	1.091SerTrp: 1.091 ± 0.014	2.217SerTyr: 2.217 ± 0.015	0.006SerXaa: 0.006 ± 0.001
Thr	3.745ThrAla: 3.745 ± 0.025	1.384ThrCys: 1.384 ± 0.017	2.676ThrAsp: 2.676 ± 0.018	3.698ThrGlu: 3.698 ± 0.02	2.14ThrPhe: 2.14 ± 0.018	3.657ThrGly: 3.657 ± 0.027	1.389ThrHis: 1.389 ± 0.023	2.708ThrIle: 2.708 ± 0.02	2.906ThrLys: 2.906 ± 0.022	5.197ThrLeu: 5.197 ± 0.027	1.161ThrMet: 1.161 ± 0.012	2.038ThrAsn: 2.038 ± 0.016	3.183ThrPro: 3.183 ± 0.033	2.191ThrGln: 2.191 ± 0.018	2.481ThrArg: 2.481 ± 0.022	4.73ThrSer: 4.73 ± 0.038	3.105ThrThr: 3.105 ± 0.042	4.017ThrVal: 4.017 ± 0.031	0.707ThrTrp: 0.707 ± 0.012	1.524ThrTyr: 1.524 ± 0.014	0.006ThrXaa: 0.006 ± 0.001
Val	3.99ValAla: 3.99 ± 0.029	1.678ValCys: 1.678 ± 0.028	2.972ValAsp: 2.972 ± 0.026	3.801ValGlu: 3.801 ± 0.023	2.461ValPhe: 2.461 ± 0.02	3.28ValGly: 3.28 ± 0.024	1.585ValHis: 1.585 ± 0.012	3.228ValIle: 3.228 ± 0.02	3.672ValLys: 3.672 ± 0.029	6.053ValLeu: 6.053 ± 0.026	1.407ValMet: 1.407 ± 0.013	2.521ValAsn: 2.521 ± 0.019	3.46ValPro: 3.46 ± 0.037	2.785ValGln: 2.785 ± 0.02	2.962ValArg: 2.962 ± 0.034	5.079ValSer: 5.079 ± 0.032	3.818ValThr: 3.818 ± 0.033	4.02ValVal: 4.02 ± 0.035	0.72ValTrp: 0.72 ± 0.01	1.727ValTyr: 1.727 ± 0.015	0.005ValXaa: 0.005 ± 0.001
Trp	0.786TrpAla: 0.786 ± 0.015	0.256TrpCys: 0.256 ± 0.008	0.647TrpAsp: 0.647 ± 0.01	0.814TrpGlu: 0.814 ± 0.011	0.465TrpPhe: 0.465 ± 0.007	0.75TrpGly: 0.75 ± 0.017	0.308TrpHis: 0.308 ± 0.007	0.656TrpIle: 0.656 ± 0.008	0.945TrpLys: 0.945 ± 0.01	1.284TrpLeu: 1.284 ± 0.014	0.319TrpMet: 0.319 ± 0.006	0.586TrpAsn: 0.586 ± 0.008	0.461TrpPro: 0.461 ± 0.008	0.554TrpGln: 0.554 ± 0.01	0.719TrpArg: 0.719 ± 0.009	0.909TrpSer: 0.909 ± 0.015	0.666TrpThr: 0.666 ± 0.01	0.72TrpVal: 0.72 ± 0.009	0.2TrpTrp: 0.2 ± 0.005	0.356TrpTyr: 0.356 ± 0.007	0.002TrpXaa: 0.002 ± 0.0
Tyr	1.466TyrAla: 1.466 ± 0.013	0.77TyrCys: 0.77 ± 0.011	1.383TyrAsp: 1.383 ± 0.013	1.722TyrGlu: 1.722 ± 0.015	1.357TyrPhe: 1.357 ± 0.013	1.722TyrGly: 1.722 ± 0.018	0.773TyrHis: 0.773 ± 0.01	1.59TyrIle: 1.59 ± 0.014	1.766TyrLys: 1.766 ± 0.019	2.813TyrLeu: 2.813 ± 0.018	0.66TyrMet: 0.66 ± 0.008	1.285TyrAsn: 1.285 ± 0.013	1.331TyrPro: 1.331 ± 0.015	1.268TyrGln: 1.268 ± 0.012	1.66TyrArg: 1.66 ± 0.013	2.348TyrSer: 2.348 ± 0.018	1.606TyrThr: 1.606 ± 0.014	1.634TyrVal: 1.634 ± 0.014	0.402TyrTrp: 0.402 ± 0.008	1.047TyrTyr: 1.047 ± 0.011	0.002TyrXaa: 0.002 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.001	0.003XaaCys: 0.003 ± 0.001	0.002XaaAsp: 0.002 ± 0.001	0.005XaaGlu: 0.005 ± 0.001	0.004XaaPhe: 0.004 ± 0.001	0.007XaaGly: 0.007 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.003XaaIle: 0.003 ± 0.0	0.005XaaLys: 0.005 ± 0.001	0.009XaaLeu: 0.009 ± 0.001	0.009XaaMet: 0.009 ± 0.001	0.002XaaAsn: 0.002 ± 0.0	0.008XaaPro: 0.008 ± 0.001	0.003XaaGln: 0.003 ± 0.001	0.007XaaArg: 0.007 ± 0.001	0.008XaaSer: 0.008 ± 0.001	0.004XaaThr: 0.004 ± 0.001	0.005XaaVal: 0.005 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.002XaaTyr: 0.002 ± 0.0	0.566XaaXaa: 0.566 ± 0.03

Statistics based on 20509 proteins (10850553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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