Streptococcus phage M102AD

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A247D730|A0A247D730_9CAUD Putative endolysin OS=Streptococcus phage M102AD OX=1587907 PE=4 SV=1
MM1 pKa = 7.29FKK3 pKa = 10.45ISIGDD8 pKa = 3.91DD9 pKa = 3.73VICYY13 pKa = 9.69QGNLRR18 pKa = 11.84DD19 pKa = 4.24ALLDD23 pKa = 4.11TIDD26 pKa = 4.17FLTAQRR32 pKa = 11.84HH33 pKa = 4.61EE34 pKa = 4.55CVGAHH39 pKa = 5.72VVLKK43 pKa = 10.56DD44 pKa = 3.58YY45 pKa = 10.9TDD47 pKa = 3.4EE48 pKa = 4.61DD49 pKa = 4.79LIEE52 pKa = 4.95EE53 pKa = 4.27IQSLTEE59 pKa = 3.75SRR61 pKa = 11.84LTISVALKK69 pKa = 10.57LDD71 pKa = 4.22DD72 pKa = 3.97NVSVVQEE79 pKa = 3.98MLEE82 pKa = 3.95

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7YZ94|G7YZ94_9CAUD Uncharacterized protein OS=Streptococcus phage M102AD OX=1587907 PE=4 SV=1
MM1 pKa = 7.29SPVLIFLQYY10 pKa = 8.45YY11 pKa = 9.41LYY13 pKa = 11.01VIFLPFFSIKK23 pKa = 10.57SVIKK27 pKa = 9.45KK28 pKa = 9.44CKK30 pKa = 9.77SFFRR34 pKa = 11.84FYY36 pKa = 10.84FLKK39 pKa = 10.67KK40 pKa = 9.97FLRR43 pKa = 11.84PPGLFF48 pKa = 3.34

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

9550

48

1733

238.8

26.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.304 ± 0.469

0.555 ± 0.135

6.681 ± 0.511

6.817 ± 0.518

4.576 ± 0.263

5.476 ± 0.598

1.079 ± 0.121

6.387 ± 0.262

9.246 ± 0.641

8.471 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.153

5.738 ± 0.267

2.366 ± 0.206

4.251 ± 0.27

4.262 ± 0.301

6.775 ± 0.394

6.45 ± 0.326

5.916 ± 0.257

0.963 ± 0.106

3.717 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski