Amino acid dipepetide frequency for Streptococcus phage M102AD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.199AlaAla: 2.199 ± 0.704
0.314AlaCys: 0.314 ± 0.231
4.608AlaAsp: 4.608 ± 0.611
6.912AlaGlu: 6.912 ± 1.075
3.875AlaPhe: 3.875 ± 1.008
3.875AlaGly: 3.875 ± 0.947
0.838AlaHis: 0.838 ± 0.328
7.435AlaIle: 7.435 ± 1.019
9.425AlaLys: 9.425 ± 0.987
6.912AlaLeu: 6.912 ± 1.041
1.99AlaMet: 1.99 ± 0.52
3.77AlaAsn: 3.77 ± 0.599
2.723AlaPro: 2.723 ± 0.628
4.922AlaGln: 4.922 ± 0.758
3.246AlaArg: 3.246 ± 0.462
6.283AlaSer: 6.283 ± 0.923
5.027AlaThr: 5.027 ± 0.597
5.131AlaVal: 5.131 ± 0.868
0.314AlaTrp: 0.314 ± 0.161
3.246AlaTyr: 3.246 ± 0.707
0.0AlaXaa: 0.0 ± 0.0
Cys
0.419CysAla: 0.419 ± 0.226
0.0CysCys: 0.0 ± 0.0
0.419CysAsp: 0.419 ± 0.211
0.314CysGlu: 0.314 ± 0.186
0.209CysPhe: 0.209 ± 0.148
0.314CysGly: 0.314 ± 0.184
0.209CysHis: 0.209 ± 0.146
0.628CysIle: 0.628 ± 0.298
0.733CysLys: 0.733 ± 0.371
0.105CysLeu: 0.105 ± 0.105
0.0CysMet: 0.0 ± 0.0
0.419CysAsn: 0.419 ± 0.196
0.209CysPro: 0.209 ± 0.163
0.314CysGln: 0.314 ± 0.17
0.209CysArg: 0.209 ± 0.158
0.105CysSer: 0.105 ± 0.11
0.0CysThr: 0.0 ± 0.0
0.524CysVal: 0.524 ± 0.269
0.105CysTrp: 0.105 ± 0.102
0.314CysTyr: 0.314 ± 0.213
0.0CysXaa: 0.0 ± 0.0
Asp
3.77AspAla: 3.77 ± 0.774
0.733AspCys: 0.733 ± 0.29
6.493AspAsp: 6.493 ± 1.318
5.341AspGlu: 5.341 ± 1.052
4.084AspPhe: 4.084 ± 0.723
3.979AspGly: 3.979 ± 0.56
0.105AspHis: 0.105 ± 0.095
3.875AspIle: 3.875 ± 0.83
5.969AspLys: 5.969 ± 0.814
4.713AspLeu: 4.713 ± 1.063
0.733AspMet: 0.733 ± 0.221
4.608AspAsn: 4.608 ± 1.007
2.409AspPro: 2.409 ± 0.612
1.047AspGln: 1.047 ± 0.362
2.513AspArg: 2.513 ± 0.469
4.608AspSer: 4.608 ± 0.593
3.142AspThr: 3.142 ± 0.808
3.875AspVal: 3.875 ± 0.707
0.838AspTrp: 0.838 ± 0.271
3.979AspTyr: 3.979 ± 0.775
0.0AspXaa: 0.0 ± 0.0
Glu
7.331GluAla: 7.331 ± 1.199
0.628GluCys: 0.628 ± 0.229
2.618GluAsp: 2.618 ± 0.465
4.713GluGlu: 4.713 ± 1.046
2.723GluPhe: 2.723 ± 0.526
2.513GluGly: 2.513 ± 0.51
0.524GluHis: 0.524 ± 0.188
5.236GluIle: 5.236 ± 1.065
5.236GluLys: 5.236 ± 0.668
8.273GluLeu: 8.273 ± 1.203
1.885GluMet: 1.885 ± 0.375
4.608GluAsn: 4.608 ± 0.743
1.152GluPro: 1.152 ± 0.43
3.037GluGln: 3.037 ± 0.529
3.142GluArg: 3.142 ± 0.701
3.351GluSer: 3.351 ± 0.55
3.875GluThr: 3.875 ± 0.807
3.979GluVal: 3.979 ± 0.665
0.733GluTrp: 0.733 ± 0.227
2.618GluTyr: 2.618 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
3.875PheAla: 3.875 ± 0.687
0.209PheCys: 0.209 ± 0.175
4.294PheAsp: 4.294 ± 0.753
3.142PheGlu: 3.142 ± 0.565
2.199PhePhe: 2.199 ± 0.487
3.456PheGly: 3.456 ± 0.654
0.314PheHis: 0.314 ± 0.183
1.885PheIle: 1.885 ± 0.368
4.398PheLys: 4.398 ± 0.604
3.875PheLeu: 3.875 ± 1.118
1.466PheMet: 1.466 ± 0.356
3.037PheAsn: 3.037 ± 0.556
0.524PhePro: 0.524 ± 0.258
0.838PheGln: 0.838 ± 0.333
1.99PheArg: 1.99 ± 0.531
2.723PheSer: 2.723 ± 0.508
2.199PheThr: 2.199 ± 0.432
2.618PheVal: 2.618 ± 0.486
0.524PheTrp: 0.524 ± 0.271
2.094PheTyr: 2.094 ± 0.55
0.0PheXaa: 0.0 ± 0.0
Gly
4.294GlyAla: 4.294 ± 0.799
0.105GlyCys: 0.105 ± 0.116
2.828GlyAsp: 2.828 ± 0.604
1.885GlyGlu: 1.885 ± 0.379
2.723GlyPhe: 2.723 ± 0.476
3.665GlyGly: 3.665 ± 0.962
0.838GlyHis: 0.838 ± 0.29
3.037GlyIle: 3.037 ± 0.793
5.55GlyLys: 5.55 ± 1.398
4.922GlyLeu: 4.922 ± 0.712
1.257GlyMet: 1.257 ± 0.257
3.246GlyAsn: 3.246 ± 0.496
0.419GlyPro: 0.419 ± 0.19
2.409GlyGln: 2.409 ± 0.588
2.199GlyArg: 2.199 ± 0.475
3.875GlySer: 3.875 ± 0.82
5.131GlyThr: 5.131 ± 0.47
2.828GlyVal: 2.828 ± 0.68
0.628GlyTrp: 0.628 ± 0.334
2.932GlyTyr: 2.932 ± 0.657
0.0GlyXaa: 0.0 ± 0.0
His
0.943HisAla: 0.943 ± 0.324
0.105HisCys: 0.105 ± 0.105
0.733HisAsp: 0.733 ± 0.317
0.838HisGlu: 0.838 ± 0.269
0.419HisPhe: 0.419 ± 0.195
0.943HisGly: 0.943 ± 0.325
0.209HisHis: 0.209 ± 0.129
0.419HisIle: 0.419 ± 0.213
0.628HisLys: 0.628 ± 0.238
1.152HisLeu: 1.152 ± 0.329
0.105HisMet: 0.105 ± 0.106
0.524HisAsn: 0.524 ± 0.217
0.209HisPro: 0.209 ± 0.124
0.733HisGln: 0.733 ± 0.244
0.524HisArg: 0.524 ± 0.178
0.524HisSer: 0.524 ± 0.196
0.628HisThr: 0.628 ± 0.313
0.419HisVal: 0.419 ± 0.206
0.209HisTrp: 0.209 ± 0.126
0.524HisTyr: 0.524 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
4.817IleAla: 4.817 ± 0.662
0.524IleCys: 0.524 ± 0.238
5.027IleAsp: 5.027 ± 0.964
5.655IleGlu: 5.655 ± 0.973
2.723IlePhe: 2.723 ± 0.641
3.351IleGly: 3.351 ± 0.652
0.524IleHis: 0.524 ± 0.246
2.199IleIle: 2.199 ± 0.442
7.016IleLys: 7.016 ± 0.647
4.608IleLeu: 4.608 ± 0.702
1.152IleMet: 1.152 ± 0.311
3.979IleAsn: 3.979 ± 0.543
1.257IlePro: 1.257 ± 0.291
2.828IleGln: 2.828 ± 0.557
2.618IleArg: 2.618 ± 0.392
6.179IleSer: 6.179 ± 0.954
3.77IleThr: 3.77 ± 0.712
3.142IleVal: 3.142 ± 0.676
0.524IleTrp: 0.524 ± 0.266
1.99IleTyr: 1.99 ± 0.505
0.0IleXaa: 0.0 ± 0.0
Lys
10.472LysAla: 10.472 ± 1.913
0.314LysCys: 0.314 ± 0.206
5.655LysAsp: 5.655 ± 0.727
6.493LysGlu: 6.493 ± 1.031
2.723LysPhe: 2.723 ± 0.63
4.189LysGly: 4.189 ± 1.149
1.466LysHis: 1.466 ± 0.482
6.179LysIle: 6.179 ± 0.925
9.006LysLys: 9.006 ± 1.059
6.598LysLeu: 6.598 ± 0.612
2.723LysMet: 2.723 ± 0.562
6.179LysAsn: 6.179 ± 0.628
2.618LysPro: 2.618 ± 0.499
4.189LysGln: 4.189 ± 0.729
2.932LysArg: 2.932 ± 0.593
5.969LysSer: 5.969 ± 0.975
6.598LysThr: 6.598 ± 0.95
3.979LysVal: 3.979 ± 0.526
0.943LysTrp: 0.943 ± 0.317
3.246LysTyr: 3.246 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
7.331LeuAla: 7.331 ± 0.833
0.209LeuCys: 0.209 ± 0.165
5.864LeuAsp: 5.864 ± 0.914
7.54LeuGlu: 7.54 ± 1.166
1.99LeuPhe: 1.99 ± 0.366
4.294LeuGly: 4.294 ± 0.631
0.733LeuHis: 0.733 ± 0.284
5.131LeuIle: 5.131 ± 0.761
9.844LeuLys: 9.844 ± 0.985
6.598LeuLeu: 6.598 ± 1.033
1.99LeuMet: 1.99 ± 0.567
4.608LeuAsn: 4.608 ± 0.487
1.885LeuPro: 1.885 ± 0.338
3.77LeuGln: 3.77 ± 0.538
2.932LeuArg: 2.932 ± 0.653
5.341LeuSer: 5.341 ± 0.552
7.016LeuThr: 7.016 ± 1.035
4.608LeuVal: 4.608 ± 0.643
0.419LeuTrp: 0.419 ± 0.225
2.618LeuTyr: 2.618 ± 0.547
0.0LeuXaa: 0.0 ± 0.0
Met
1.99MetAla: 1.99 ± 0.495
0.209MetCys: 0.209 ± 0.171
1.152MetAsp: 1.152 ± 0.436
1.152MetGlu: 1.152 ± 0.434
0.943MetPhe: 0.943 ± 0.289
1.047MetGly: 1.047 ± 0.262
0.209MetHis: 0.209 ± 0.142
1.571MetIle: 1.571 ± 0.478
1.466MetLys: 1.466 ± 0.445
2.199MetLeu: 2.199 ± 0.498
0.105MetMet: 0.105 ± 0.111
0.733MetAsn: 0.733 ± 0.34
0.628MetPro: 0.628 ± 0.275
1.152MetGln: 1.152 ± 0.383
0.628MetArg: 0.628 ± 0.326
1.257MetSer: 1.257 ± 0.309
1.361MetThr: 1.361 ± 0.286
0.733MetVal: 0.733 ± 0.236
0.314MetTrp: 0.314 ± 0.157
0.838MetTyr: 0.838 ± 0.37
0.0MetXaa: 0.0 ± 0.0
Asn
5.236AsnAla: 5.236 ± 0.671
0.209AsnCys: 0.209 ± 0.143
3.351AsnAsp: 3.351 ± 0.663
2.409AsnGlu: 2.409 ± 0.575
4.503AsnPhe: 4.503 ± 0.663
3.77AsnGly: 3.77 ± 0.722
0.628AsnHis: 0.628 ± 0.214
3.351AsnIle: 3.351 ± 0.63
4.398AsnLys: 4.398 ± 0.659
4.608AsnLeu: 4.608 ± 0.665
0.943AsnMet: 0.943 ± 0.329
2.723AsnAsn: 2.723 ± 0.631
1.257AsnPro: 1.257 ± 0.443
2.618AsnGln: 2.618 ± 0.504
2.723AsnArg: 2.723 ± 0.49
4.084AsnSer: 4.084 ± 0.571
3.561AsnThr: 3.561 ± 0.705
3.456AsnVal: 3.456 ± 0.582
1.047AsnTrp: 1.047 ± 0.289
2.513AsnTyr: 2.513 ± 0.42
0.0AsnXaa: 0.0 ± 0.0
Pro
2.723ProAla: 2.723 ± 0.589
0.0ProCys: 0.0 ± 0.0
1.466ProAsp: 1.466 ± 0.402
1.78ProGlu: 1.78 ± 0.37
1.885ProPhe: 1.885 ± 0.456
1.047ProGly: 1.047 ± 0.334
0.105ProHis: 0.105 ± 0.086
1.047ProIle: 1.047 ± 0.293
1.78ProLys: 1.78 ± 0.383
1.885ProLeu: 1.885 ± 0.308
0.105ProMet: 0.105 ± 0.108
0.943ProAsn: 0.943 ± 0.348
0.524ProPro: 0.524 ± 0.25
1.361ProGln: 1.361 ± 0.408
1.152ProArg: 1.152 ± 0.355
1.571ProSer: 1.571 ± 0.524
1.257ProThr: 1.257 ± 0.273
1.78ProVal: 1.78 ± 0.573
0.524ProTrp: 0.524 ± 0.248
0.733ProTyr: 0.733 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
3.561GlnAla: 3.561 ± 0.525
0.105GlnCys: 0.105 ± 0.1
1.885GlnAsp: 1.885 ± 0.532
3.561GlnGlu: 3.561 ± 0.594
0.838GlnPhe: 0.838 ± 0.36
1.571GlnGly: 1.571 ± 0.353
0.314GlnHis: 0.314 ± 0.175
2.932GlnIle: 2.932 ± 0.587
5.131GlnLys: 5.131 ± 1.015
3.037GlnLeu: 3.037 ± 0.463
0.943GlnMet: 0.943 ± 0.232
3.456GlnAsn: 3.456 ± 0.486
0.943GlnPro: 0.943 ± 0.35
1.571GlnGln: 1.571 ± 0.541
2.828GlnArg: 2.828 ± 0.459
3.561GlnSer: 3.561 ± 0.73
3.142GlnThr: 3.142 ± 0.671
2.199GlnVal: 2.199 ± 0.431
0.314GlnTrp: 0.314 ± 0.178
0.628GlnTyr: 0.628 ± 0.328
0.0GlnXaa: 0.0 ± 0.0
Arg
3.77ArgAla: 3.77 ± 0.843
0.314ArgCys: 0.314 ± 0.173
2.409ArgAsp: 2.409 ± 0.499
3.665ArgGlu: 3.665 ± 0.577
2.199ArgPhe: 2.199 ± 0.499
1.885ArgGly: 1.885 ± 0.355
0.733ArgHis: 0.733 ± 0.273
3.142ArgIle: 3.142 ± 0.657
2.304ArgLys: 2.304 ± 0.634
4.189ArgLeu: 4.189 ± 0.767
0.733ArgMet: 0.733 ± 0.27
2.828ArgAsn: 2.828 ± 0.58
1.466ArgPro: 1.466 ± 0.416
1.361ArgGln: 1.361 ± 0.365
2.723ArgArg: 2.723 ± 0.498
2.513ArgSer: 2.513 ± 0.527
1.676ArgThr: 1.676 ± 0.3
2.828ArgVal: 2.828 ± 0.452
0.524ArgTrp: 0.524 ± 0.202
1.361ArgTyr: 1.361 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
6.912SerAla: 6.912 ± 1.227
0.314SerCys: 0.314 ± 0.168
6.493SerAsp: 6.493 ± 1.095
4.713SerGlu: 4.713 ± 0.778
3.875SerPhe: 3.875 ± 0.694
4.398SerGly: 4.398 ± 1.012
0.943SerHis: 0.943 ± 0.322
3.246SerIle: 3.246 ± 0.53
6.179SerLys: 6.179 ± 1.048
4.713SerLeu: 4.713 ± 0.732
1.152SerMet: 1.152 ± 0.336
3.246SerAsn: 3.246 ± 0.57
1.152SerPro: 1.152 ± 0.41
2.723SerGln: 2.723 ± 0.436
3.037SerArg: 3.037 ± 0.531
3.875SerSer: 3.875 ± 0.984
4.713SerThr: 4.713 ± 0.681
3.875SerVal: 3.875 ± 0.516
0.105SerTrp: 0.105 ± 0.105
2.094SerTyr: 2.094 ± 0.488
0.0SerXaa: 0.0 ± 0.0
Thr
6.283ThrAla: 6.283 ± 0.795
0.419ThrCys: 0.419 ± 0.19
5.131ThrAsp: 5.131 ± 0.667
3.456ThrGlu: 3.456 ± 0.493
2.723ThrPhe: 2.723 ± 0.513
3.875ThrGly: 3.875 ± 0.852
0.733ThrHis: 0.733 ± 0.273
4.713ThrIle: 4.713 ± 0.63
5.131ThrLys: 5.131 ± 0.746
6.598ThrLeu: 6.598 ± 1.113
0.524ThrMet: 0.524 ± 0.254
3.665ThrAsn: 3.665 ± 0.892
1.466ThrPro: 1.466 ± 0.333
3.351ThrGln: 3.351 ± 0.784
2.618ThrArg: 2.618 ± 0.419
3.561ThrSer: 3.561 ± 0.601
3.351ThrThr: 3.351 ± 0.752
4.713ThrVal: 4.713 ± 0.691
0.314ThrTrp: 0.314 ± 0.165
1.885ThrTyr: 1.885 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
3.875ValAla: 3.875 ± 0.578
0.524ValCys: 0.524 ± 0.244
4.084ValAsp: 4.084 ± 0.749
2.618ValGlu: 2.618 ± 0.442
2.409ValPhe: 2.409 ± 0.488
3.561ValGly: 3.561 ± 0.575
0.628ValHis: 0.628 ± 0.247
4.608ValIle: 4.608 ± 0.776
4.608ValLys: 4.608 ± 0.803
5.236ValLeu: 5.236 ± 0.666
1.047ValMet: 1.047 ± 0.25
2.828ValAsn: 2.828 ± 0.648
1.78ValPro: 1.78 ± 0.381
2.513ValGln: 2.513 ± 0.587
1.78ValArg: 1.78 ± 0.357
4.817ValSer: 4.817 ± 0.673
4.713ValThr: 4.713 ± 1.045
4.608ValVal: 4.608 ± 0.784
0.733ValTrp: 0.733 ± 0.253
2.199ValTyr: 2.199 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
0.314TrpAla: 0.314 ± 0.195
0.105TrpCys: 0.105 ± 0.114
0.105TrpAsp: 0.105 ± 0.086
0.628TrpGlu: 0.628 ± 0.262
0.419TrpPhe: 0.419 ± 0.194
0.628TrpGly: 0.628 ± 0.248
0.0TrpHis: 0.0 ± 0.0
0.733TrpIle: 0.733 ± 0.31
0.838TrpLys: 0.838 ± 0.322
1.257TrpLeu: 1.257 ± 0.419
0.314TrpMet: 0.314 ± 0.171
0.314TrpAsn: 0.314 ± 0.132
0.105TrpPro: 0.105 ± 0.111
0.105TrpGln: 0.105 ± 0.127
0.733TrpArg: 0.733 ± 0.237
0.733TrpSer: 0.733 ± 0.23
1.047TrpThr: 1.047 ± 0.356
0.733TrpVal: 0.733 ± 0.335
0.209TrpTrp: 0.209 ± 0.181
0.314TrpTyr: 0.314 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.932TyrAla: 2.932 ± 0.443
0.209TyrCys: 0.209 ± 0.152
2.199TyrAsp: 2.199 ± 0.473
1.361TyrGlu: 1.361 ± 0.376
2.304TyrPhe: 2.304 ± 0.458
2.409TyrGly: 2.409 ± 0.591
0.733TyrHis: 0.733 ± 0.281
2.513TyrIle: 2.513 ± 0.65
2.828TyrLys: 2.828 ± 0.493
3.246TyrLeu: 3.246 ± 0.621
0.419TyrMet: 0.419 ± 0.206
1.676TyrAsn: 1.676 ± 0.354
0.943TyrPro: 0.943 ± 0.375
1.676TyrGln: 1.676 ± 0.399
2.094TyrArg: 2.094 ± 0.404
2.828TyrSer: 2.828 ± 0.402
2.304TyrThr: 2.304 ± 0.291
3.142TyrVal: 3.142 ± 0.789
0.314TyrTrp: 0.314 ± 0.232
1.047TyrTyr: 1.047 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (9550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski