Arhar cryptic virus-I

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5LY29|X5LY29_9VIRU Isoform of X5M5C8 Coat Protein OS=Arhar cryptic virus-I OX=1585924 GN=CP PE=4 SV=1
MM1 pKa = 7.31AAEE4 pKa = 5.26GPAQPSLTEE13 pKa = 4.42SGASSPNPPVVKK25 pKa = 9.82RR26 pKa = 11.84DD27 pKa = 3.52EE28 pKa = 5.39SKK30 pKa = 10.96DD31 pKa = 3.51QPKK34 pKa = 10.81ASIGSTFRR42 pKa = 11.84MKK44 pKa = 10.97NEE46 pKa = 3.63APVAQKK52 pKa = 10.88LVVALLDD59 pKa = 3.64QEE61 pKa = 4.8TVDD64 pKa = 4.76IPAAGNQNYY73 pKa = 9.95IIEE76 pKa = 4.4LKK78 pKa = 10.65EE79 pKa = 3.88KK80 pKa = 10.78GILQHH85 pKa = 6.81RR86 pKa = 11.84FPRR89 pKa = 11.84DD90 pKa = 3.28RR91 pKa = 11.84ISTVCEE97 pKa = 4.51PIPMNMQNLFTGALITIRR115 pKa = 11.84ASLTRR120 pKa = 11.84LLQLKK125 pKa = 7.37TQLGDD130 pKa = 3.13EE131 pKa = 4.64DD132 pKa = 5.0YY133 pKa = 8.17EE134 pKa = 4.32TRR136 pKa = 11.84LDD138 pKa = 3.78VVATNLAIGCNLVTFMKK155 pKa = 10.46LRR157 pKa = 11.84ALALTDD163 pKa = 3.21VEE165 pKa = 4.72TRR167 pKa = 11.84HH168 pKa = 5.98NADD171 pKa = 3.01GLRR174 pKa = 11.84RR175 pKa = 11.84PRR177 pKa = 11.84AIADD181 pKa = 3.56VPIPTAFAFAIQQLGIVNIADD202 pKa = 3.72LTHH205 pKa = 6.21EE206 pKa = 4.54LKK208 pKa = 10.55FVPCLPQPGYY218 pKa = 10.92QFGLPQTLTWNPNAYY233 pKa = 8.84QQAVEE238 pKa = 4.19YY239 pKa = 10.58ARR241 pKa = 11.84SLGMRR246 pKa = 11.84FSIVDD251 pKa = 3.5TTVKK255 pKa = 10.55EE256 pKa = 4.65GTAWWLFEE264 pKa = 3.61QSYY267 pKa = 10.36NEE269 pKa = 3.95NVFEE273 pKa = 4.75LMCPYY278 pKa = 10.23PEE280 pKa = 4.81SNFTEE285 pKa = 4.53MMALAHH291 pKa = 6.18SLYY294 pKa = 10.68LAGDD298 pKa = 3.54EE299 pKa = 4.71ADD301 pKa = 4.08PSNTLLNVEE310 pKa = 4.7DD311 pKa = 4.37MPHH314 pKa = 6.13GGNYY318 pKa = 9.76GIMMQFPHH326 pKa = 7.74LGIHH330 pKa = 6.43LSSFEE335 pKa = 4.26ALSEE339 pKa = 3.98AASDD343 pKa = 2.86IWSNVV348 pKa = 2.77

Molecular weight: 38.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5LY29|X5LY29_9VIRU Isoform of X5M5C8 Coat Protein OS=Arhar cryptic virus-I OX=1585924 GN=CP PE=4 SV=1
MM1 pKa = 7.33VDD3 pKa = 3.03KK4 pKa = 10.97RR5 pKa = 11.84KK6 pKa = 8.82NTDD9 pKa = 3.06SQFASPTTAKK19 pKa = 9.37TPKK22 pKa = 9.56IVTPGEE28 pKa = 3.89PSNNNPALSIVTHH41 pKa = 6.75RR42 pKa = 11.84GASQAEE48 pKa = 4.58SIRR51 pKa = 11.84PEE53 pKa = 4.12AEE55 pKa = 3.96LYY57 pKa = 9.71PVQTKK62 pKa = 10.51RR63 pKa = 11.84LVLEE67 pKa = 4.79GNPSYY72 pKa = 10.71YY73 pKa = 10.23RR74 pKa = 11.84KK75 pKa = 10.27LLDD78 pKa = 3.77RR79 pKa = 11.84KK80 pKa = 9.5EE81 pKa = 3.52IGYY84 pKa = 9.97RR85 pKa = 11.84RR86 pKa = 11.84TGAGKK91 pKa = 10.1VLTFRR96 pKa = 11.84SFDD99 pKa = 3.89LNVSYY104 pKa = 10.54FRR106 pKa = 11.84TAITDD111 pKa = 3.18VMSRR115 pKa = 11.84VLKK118 pKa = 10.31IRR120 pKa = 11.84YY121 pKa = 8.43QSNGSMRR128 pKa = 11.84QTEE131 pKa = 4.43IDD133 pKa = 3.53TEE135 pKa = 3.99IATFVPHH142 pKa = 6.6IVNVCMSALYY152 pKa = 10.57AKK154 pKa = 10.21LRR156 pKa = 11.84SLNKK160 pKa = 9.77SYY162 pKa = 10.73GRR164 pKa = 11.84HH165 pKa = 4.0GTRR168 pKa = 11.84YY169 pKa = 8.13ATAPVYY175 pKa = 10.44TKK177 pKa = 10.31DD178 pKa = 3.58IEE180 pKa = 4.51IPLPLALAIQEE191 pKa = 4.37LGSFQTQNLEE201 pKa = 4.08NNVIMIPTYY210 pKa = 10.15PEE212 pKa = 4.53GVQHH216 pKa = 6.93EE217 pKa = 4.72GRR219 pKa = 11.84TAAEE223 pKa = 4.22FPVAEE228 pKa = 4.24YY229 pKa = 10.73LSYY232 pKa = 11.41VPTLLDD238 pKa = 3.7LKK240 pKa = 10.73IPLKK244 pKa = 10.99SVDD247 pKa = 3.32ARR249 pKa = 11.84IKK251 pKa = 9.88TGSAWWTYY259 pKa = 8.79KK260 pKa = 10.92LEE262 pKa = 4.62VIHH265 pKa = 5.55YY266 pKa = 6.81TADD269 pKa = 4.97FICTIPRR276 pKa = 11.84SLYY279 pKa = 10.8SDD281 pKa = 3.13MTALLRR287 pKa = 11.84TLFLSGSLEE296 pKa = 3.95QPPEE300 pKa = 4.03DD301 pKa = 3.24LVVFPEE307 pKa = 4.2DD308 pKa = 3.06QATFGTLLNCLSAGFNARR326 pKa = 11.84TFLALIGGPKK336 pKa = 9.85EE337 pKa = 3.78EE338 pKa = 4.55WSFGTNN344 pKa = 2.74

Molecular weight: 38.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

1167

344

475

389.0

44.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.055 ± 0.628

0.943 ± 0.075

4.799 ± 0.403

6.512 ± 0.151

4.37 ± 0.234

4.97 ± 0.377

2.485 ± 0.555

6.17 ± 0.392

4.113 ± 0.362

10.026 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.373

4.37 ± 0.45

5.57 ± 0.785

3.599 ± 0.583

6.255 ± 0.841

7.112 ± 0.535

6.341 ± 1.099

5.998 ± 0.089

1.457 ± 0.327

4.456 ± 0.736
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski